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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20590
         (640 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ...    55   3e-08
At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical...    54   1e-07
At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ...    49   3e-06
At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ...    34   0.092
At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-...    34   0.092
At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ...    31   0.65 

>At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852 from
           [Arabidopsis thaliana]; identical to cDNA small
           ubiquitin-like modifier 2 (SUMO)  GI:22652843; contains
           Pfam profile PF00240: Ubiquitin family
          Length = 103

 Score = 55.2 bits (127), Expect = 3e-08
 Identities = 25/42 (59%), Positives = 31/42 (73%)
 Frame = +1

Query: 121 DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMHAYCDR 246
           D+K  +  HINLKV GQD   V F+IK+ T L+KLM+AYCDR
Sbjct: 8   DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDR 49



 Score = 41.5 bits (93), Expect = 5e-04
 Identities = 17/36 (47%), Positives = 22/36 (61%)
 Frame = +3

Query: 267 VRFRFDGQPINGNDTPTSLEMEEGDTIEVYQQQTGG 374
           + F FDG+ +    TP  LEME+GD I+    QTGG
Sbjct: 57  IAFLFDGRRLRAEQTPDELEMEDGDEIDAMLHQTGG 92


>At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to
           Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis
           thaliana]; identical to cDNA SMT3 protein GI:1707371
          Length = 100

 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 26/43 (60%), Positives = 32/43 (74%), Gaps = 1/43 (2%)
 Frame = +1

Query: 121 DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMHAYCDR 246
           D+K G+   HINLKV GQD   V F+IK+ T L+KLM+AYCDR
Sbjct: 8   DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDR 50



 Score = 41.5 bits (93), Expect = 5e-04
 Identities = 17/39 (43%), Positives = 23/39 (58%)
 Frame = +3

Query: 258 MQVVRFRFDGQPINGNDTPTSLEMEEGDTIEVYQQQTGG 374
           M  + F FDG+ +    TP  L+ME+GD I+    QTGG
Sbjct: 55  MNSIAFLFDGRRLRAEQTPDELDMEDGDEIDAMLHQTGG 93


>At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO)
           similar to SP|O13351 Ubiquitin-like protein smt3/pmt3
           {Schizosaccharomyces pombe}; identical to cDNA small
           ubiquitin-like modifier 3 (SUMO) GI:22652845
          Length = 111

 Score = 48.8 bits (111), Expect = 3e-06
 Identities = 22/41 (53%), Positives = 27/41 (65%)
 Frame = +1

Query: 136 ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMHAYCDRAGLQ 258
           +  H+ LKV  QD   V FK KK  PL+KLM+ YCDR GL+
Sbjct: 14  QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLK 54



 Score = 36.7 bits (81), Expect = 0.013
 Identities = 15/35 (42%), Positives = 23/35 (65%)
 Frame = +3

Query: 273 FRFDGQPINGNDTPTSLEMEEGDTIEVYQQQTGGV 377
           F F+G  I G +TP  L+ME+GD I+  +  +GG+
Sbjct: 60  FIFNGARIGGLETPDELDMEDGDVIDACRAMSGGL 94


>At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852
           [Arabidopsis thaliana]; contains INTERPRO:IPR000626
           ubiquitin domain; contains Pfam profile PF00240:
           Ubiquitin family; contains Pfam profile PF00240:
           Ubiquitin family;  identical to cDNA small
           ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847
          Length = 108

 Score = 33.9 bits (74), Expect = 0.092
 Identities = 17/51 (33%), Positives = 25/51 (49%)
 Frame = +1

Query: 103 INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMHAYCDRAGL 255
           ++ K    +   +  + LKV  Q  A   +KI  H  L+KLM AYC +  L
Sbjct: 13  VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNL 63



 Score = 32.3 bits (70), Expect = 0.28
 Identities = 15/36 (41%), Positives = 20/36 (55%)
 Frame = +3

Query: 267 VRFRFDGQPINGNDTPTSLEMEEGDTIEVYQQQTGG 374
           VRF ++G+ I    TP  L MEE D I +  +  GG
Sbjct: 68  VRFVYNGREIKARQTPAQLHMEEEDEICMVMELGGG 103


>At1g68185.1 68414.m07789 ubiquitin-related similar to
           ubiquitin-like protein smt3/pmt3 SP:O13351 from [Fission
           yeast]
          Length = 215

 Score = 33.9 bits (74), Expect = 0.092
 Identities = 15/32 (46%), Positives = 22/32 (68%)
 Frame = +3

Query: 273 FRFDGQPINGNDTPTSLEMEEGDTIEVYQQQT 368
           F FDG  I+ + TP+ L ME+ D IEV+ ++T
Sbjct: 184 FIFDGDKIDPSTTPSELGMEDHDMIEVHTKKT 215


>At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351
           Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces
           pombe}
          Length = 114

 Score = 31.1 bits (67), Expect = 0.65
 Identities = 15/35 (42%), Positives = 18/35 (51%)
 Frame = +3

Query: 270 RFRFDGQPINGNDTPTSLEMEEGDTIEVYQQQTGG 374
           RF FDG  I    TP  LE ++GD I+    Q  G
Sbjct: 70  RFLFDGSRIREYHTPDELERKDGDEIDAMLCQQSG 104


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,784,319
Number of Sequences: 28952
Number of extensions: 249896
Number of successful extensions: 429
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 415
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 429
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1314848736
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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