BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20590 (640 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ... 55 3e-08 At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical... 54 1e-07 At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ... 49 3e-06 At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ... 34 0.092 At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-... 34 0.092 At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ... 31 0.65 >At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) similar to ubiquitin-like protein SMT3 SP:P55852 from [Arabidopsis thaliana]; identical to cDNA small ubiquitin-like modifier 2 (SUMO) GI:22652843; contains Pfam profile PF00240: Ubiquitin family Length = 103 Score = 55.2 bits (127), Expect = 3e-08 Identities = 25/42 (59%), Positives = 31/42 (73%) Frame = +1 Query: 121 DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMHAYCDR 246 D+K + HINLKV GQD V F+IK+ T L+KLM+AYCDR Sbjct: 8 DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDR 49 Score = 41.5 bits (93), Expect = 5e-04 Identities = 17/36 (47%), Positives = 22/36 (61%) Frame = +3 Query: 267 VRFRFDGQPINGNDTPTSLEMEEGDTIEVYQQQTGG 374 + F FDG+ + TP LEME+GD I+ QTGG Sbjct: 57 IAFLFDGRRLRAEQTPDELEMEDGDEIDAMLHQTGG 92 >At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis thaliana]; identical to cDNA SMT3 protein GI:1707371 Length = 100 Score = 53.6 bits (123), Expect = 1e-07 Identities = 26/43 (60%), Positives = 32/43 (74%), Gaps = 1/43 (2%) Frame = +1 Query: 121 DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMHAYCDR 246 D+K G+ HINLKV GQD V F+IK+ T L+KLM+AYCDR Sbjct: 8 DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDR 50 Score = 41.5 bits (93), Expect = 5e-04 Identities = 17/39 (43%), Positives = 23/39 (58%) Frame = +3 Query: 258 MQVVRFRFDGQPINGNDTPTSLEMEEGDTIEVYQQQTGG 374 M + F FDG+ + TP L+ME+GD I+ QTGG Sbjct: 55 MNSIAFLFDGRRLRAEQTPDELDMEDGDEIDAMLHQTGG 93 >At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) similar to SP|O13351 Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces pombe}; identical to cDNA small ubiquitin-like modifier 3 (SUMO) GI:22652845 Length = 111 Score = 48.8 bits (111), Expect = 3e-06 Identities = 22/41 (53%), Positives = 27/41 (65%) Frame = +1 Query: 136 ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMHAYCDRAGLQ 258 + H+ LKV QD V FK KK PL+KLM+ YCDR GL+ Sbjct: 14 QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLK 54 Score = 36.7 bits (81), Expect = 0.013 Identities = 15/35 (42%), Positives = 23/35 (65%) Frame = +3 Query: 273 FRFDGQPINGNDTPTSLEMEEGDTIEVYQQQTGGV 377 F F+G I G +TP L+ME+GD I+ + +GG+ Sbjct: 60 FIFNGARIGGLETPDELDMEDGDVIDACRAMSGGL 94 >At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) similar to ubiquitin-like protein SMT3 SP:P55852 [Arabidopsis thaliana]; contains INTERPRO:IPR000626 ubiquitin domain; contains Pfam profile PF00240: Ubiquitin family; contains Pfam profile PF00240: Ubiquitin family; identical to cDNA small ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847 Length = 108 Score = 33.9 bits (74), Expect = 0.092 Identities = 17/51 (33%), Positives = 25/51 (49%) Frame = +1 Query: 103 INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMHAYCDRAGL 255 ++ K + + + LKV Q A +KI H L+KLM AYC + L Sbjct: 13 VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNL 63 Score = 32.3 bits (70), Expect = 0.28 Identities = 15/36 (41%), Positives = 20/36 (55%) Frame = +3 Query: 267 VRFRFDGQPINGNDTPTSLEMEEGDTIEVYQQQTGG 374 VRF ++G+ I TP L MEE D I + + GG Sbjct: 68 VRFVYNGREIKARQTPAQLHMEEEDEICMVMELGGG 103 >At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-like protein smt3/pmt3 SP:O13351 from [Fission yeast] Length = 215 Score = 33.9 bits (74), Expect = 0.092 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +3 Query: 273 FRFDGQPINGNDTPTSLEMEEGDTIEVYQQQT 368 F FDG I+ + TP+ L ME+ D IEV+ ++T Sbjct: 184 FIFDGDKIDPSTTPSELGMEDHDMIEVHTKKT 215 >At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces pombe} Length = 114 Score = 31.1 bits (67), Expect = 0.65 Identities = 15/35 (42%), Positives = 18/35 (51%) Frame = +3 Query: 270 RFRFDGQPINGNDTPTSLEMEEGDTIEVYQQQTGG 374 RF FDG I TP LE ++GD I+ Q G Sbjct: 70 RFLFDGSRIREYHTPDELERKDGDEIDAMLCQQSG 104 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,784,319 Number of Sequences: 28952 Number of extensions: 249896 Number of successful extensions: 429 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 415 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 429 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1314848736 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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