BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20588
(699 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g10840.1 68414.m01246 eukaryotic translation initiation facto... 76 2e-14
At1g10840.2 68414.m01245 eukaryotic translation initiation facto... 66 2e-11
At5g05780.1 68418.m00636 26S proteasome non-ATPase regulatory su... 32 0.42
At3g11270.1 68416.m01370 26S proteasome non-ATPase regulatory su... 32 0.42
At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2) (... 29 2.2
At5g22355.1 68418.m02608 DC1 domain-containing protein contains ... 29 3.9
At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1) (P... 29 3.9
At5g44960.1 68418.m05514 F-box family protein contains F-box dom... 28 5.2
At1g18160.1 68414.m02256 protein kinase family protein contains ... 28 5.2
At1g14750.1 68414.m01763 cyclin, putative (SDS) identical to cyc... 28 5.2
At1g11330.1 68414.m01301 S-locus lectin protein kinase family pr... 27 9.0
>At1g10840.1 68414.m01246 eukaryotic translation initiation factor 3
subunit 3 / eIF-3 gamma / eIF3h (TIF3H1) identical to
SP|Q9C5Z2 Eukaryotic translation initiation factor 3
subunit 3 (eIF-3 gamma) (eIF3 p38 subunit) (eIF3h)
{Arabidopsis thaliana}; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 337
Score = 76.2 bits (179), Expect = 2e-14
Identities = 42/79 (53%), Positives = 52/79 (65%), Gaps = 4/79 (5%)
Frame = +3
Query: 30 IQYVQCDGLAVMKIVKHCHEESCSNMEVAQGALLGLVVENRLEITNCFPFPKHDD----T 197
++ VQ +GLAV+KI+KHC E S + + G LLGL V + LE+TNCFPFP DD
Sbjct: 22 LRVVQIEGLAVLKIIKHCKEFSPT---LVTGQLLGLDVGSVLEVTNCFPFPVRDDDEEIE 78
Query: 198 MDEEEYQLDMMRRLRRVNV 254
D YQL+MMR LR VNV
Sbjct: 79 ADGANYQLEMMRCLREVNV 97
Score = 66.5 bits (155), Expect = 2e-11
Identities = 29/81 (35%), Positives = 51/81 (62%)
Frame = +2
Query: 266 VGWYQSADVGNFLSLSLLESQYHYQTSIEESVVVIYDTKKSARGFLTLKAYRLTPQAIAM 445
VGWYQS +G++ ++ L+E+ +YQ +I+ V +IYD K+ G L LKA +L+ + +
Sbjct: 102 VGWYQSTVLGSYQTVELIETFMNYQENIKRCVCIIYDPSKADLGVLALKALKLSDSFMEL 161
Query: 446 YKEGDYTPEALRNLKIGYENL 508
Y+ G++T E LR + ++
Sbjct: 162 YRGGNFTGEKLREKNFSWMDI 182
>At1g10840.2 68414.m01245 eukaryotic translation initiation factor 3
subunit 3 / eIF-3 gamma / eIF3h (TIF3H1) identical to
SP|Q9C5Z2 Eukaryotic translation initiation factor 3
subunit 3 (eIF-3 gamma) (eIF3 p38 subunit) (eIF3h)
{Arabidopsis thaliana}; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 250
Score = 66.5 bits (155), Expect = 2e-11
Identities = 29/81 (35%), Positives = 51/81 (62%)
Frame = +2
Query: 266 VGWYQSADVGNFLSLSLLESQYHYQTSIEESVVVIYDTKKSARGFLTLKAYRLTPQAIAM 445
VGWYQS +G++ ++ L+E+ +YQ +I+ V +IYD K+ G L LKA +L+ + +
Sbjct: 15 VGWYQSTVLGSYQTVELIETFMNYQENIKRCVCIIYDPSKADLGVLALKALKLSDSFMEL 74
Query: 446 YKEGDYTPEALRNLKIGYENL 508
Y+ G++T E LR + ++
Sbjct: 75 YRGGNFTGEKLREKNFSWMDI 95
>At5g05780.1 68418.m00636 26S proteasome non-ATPase regulatory
subunit 7, putative / 26S proteasome regulatory subunit
S12, putative / MOV34 protein, putative contains
similarity to 26s proteasome regulatory subunit s12
(proteasome subunit p40) (mov34 protein) SP:P26516 from
[Mus musculus]; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 308
Score = 31.9 bits (69), Expect = 0.42
Identities = 18/66 (27%), Positives = 31/66 (46%)
Frame = +3
Query: 27 TIQYVQCDGLAVMKIVKHCHEESCSNMEVAQGALLGLVVENRLEITNCFPFPKHDDTMDE 206
TI+ V L ++ IV H + + + + G LLG +++TN + P +D D
Sbjct: 13 TIEKVVVHPLVLLSIVDHYNRVAKDSSKRVVGVLLGSSSRGVVDVTNSYAVPFEEDDKDP 72
Query: 207 EEYQLD 224
+ LD
Sbjct: 73 SIWFLD 78
>At3g11270.1 68416.m01370 26S proteasome non-ATPase regulatory
subunit 7, putative / 26S proteasome regulatory subunit
S12, putative / MOV34 protein, putative contains
similarity to 26S proteasome regulatory subunit S12
(MOV34) SP:P26516 from [Mus musculus]
Length = 310
Score = 31.9 bits (69), Expect = 0.42
Identities = 18/66 (27%), Positives = 30/66 (45%)
Frame = +3
Query: 27 TIQYVQCDGLAVMKIVKHCHEESCSNMEVAQGALLGLVVENRLEITNCFPFPKHDDTMDE 206
TI+ V L ++ IV H + + + G LLG +++TN + P +D D
Sbjct: 13 TIEKVIVHPLVLLSIVDHYNRVAKDTSKRVVGVLLGSSSRGTVDVTNSYAVPFEEDDKDT 72
Query: 207 EEYQLD 224
+ LD
Sbjct: 73 SIWFLD 78
>At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2)
(PRCGA) identical to SP|O24633 Proteasome subunit beta
type 2-2 (EC 3.4.25.1) (20S proteasome alpha subunit D2)
{Arabidopsis thaliana}, cDNA proteasome subunit prcga
GI:2511571
Length = 199
Score = 29.5 bits (63), Expect = 2.2
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 5/55 (9%)
Frame = +2
Query: 221 GHDEEASKSQCYHFHVGWYQSAD-----VGNFLSLSLLESQYHYQTSIEESVVVI 370
G+D+EA S Y ++ D G++ SLS ++ Y S+EE++ ++
Sbjct: 105 GYDKEAGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELV 159
>At5g22355.1 68418.m02608 DC1 domain-containing protein contains
Pfam PF03107: DC1 domain; similar to ULI3 [Arabidopsis
thaliana] GI:17225050
Length = 664
Score = 28.7 bits (61), Expect = 3.9
Identities = 19/61 (31%), Positives = 27/61 (44%), Gaps = 1/61 (1%)
Frame = +1
Query: 262 SCWMVPECRCRQFSKPFVIRV-TISLSDLY*GECCCDLRYKEISQRILDFEGLSFDTSGY 438
S W V + RC S+P + L + + CCD YKEI +L + FD +
Sbjct: 477 SDWWVLDVRCGSRSEPVIHDGHRHPLYYEHKKDHCCDACYKEIDGYLLSCDTCDFDLDLH 536
Query: 439 C 441
C
Sbjct: 537 C 537
>At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1)
(PRGB) identical to GB:CAA74026 from [Arabidopsis
thaliana] ( FEBS Lett. (1997) 416 (3), 281-285);
identical to cDNA proteasome subunit prgb GI:2511589
Length = 204
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/55 (25%), Positives = 28/55 (50%), Gaps = 5/55 (9%)
Frame = +2
Query: 221 GHDEEASKSQCYHFHVGWYQSAD-----VGNFLSLSLLESQYHYQTSIEESVVVI 370
G+D+E+ S Y ++ D G++ SLS ++ Y S+EE++ ++
Sbjct: 105 GYDDESGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELV 159
>At5g44960.1 68418.m05514 F-box family protein contains F-box domain
Pfam:PF00646
Length = 416
Score = 28.3 bits (60), Expect = 5.2
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 5/43 (11%)
Frame = -1
Query: 693 LDRFLIQFINTFHETAKLSF-----ENRCSTQIQKLGSFLFRY 580
LD F +QF N HE + F E+ C +++QK F+ RY
Sbjct: 47 LDLFSLQFTNPHHEEGLIKFMDRFMESNCRSRLQK---FMIRY 86
>At1g18160.1 68414.m02256 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 992
Score = 28.3 bits (60), Expect = 5.2
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +3
Query: 192 DTMDEEEYQLDMMRRLRRVNVIIFMLDGTR 281
+ ++E ++ MMRRLR N+++FM TR
Sbjct: 752 EALEEFRSEVRMMRRLRHPNIVLFMGAVTR 781
>At1g14750.1 68414.m01763 cyclin, putative (SDS) identical to
cyclin-like protein [Arabidopsis thaliana] GI:20302467;
low similarity to SP|P30278 G2/mitotic-specific cyclin 2
(B-like cyclin) (CycMs2 {Medicago sativa}; contains Pfam
profiles PF00134: Cyclin, N-terminal domain, PF02984:
Cyclin, C-terminal domain
Length = 578
Score = 28.3 bits (60), Expect = 5.2
Identities = 15/40 (37%), Positives = 25/40 (62%)
Frame = -2
Query: 338 DSDIVTLITKGLENCLHLHSGTIQHENDNIDSSKPPHHVQ 219
+SD+V++I+ G+E C S T +N+ I+ SKP V+
Sbjct: 185 ESDVVSVIS-GVEYCSKFGSVTGGADNEEIEISKPSSFVE 223
>At1g11330.1 68414.m01301 S-locus lectin protein kinase family
protein contains Pfam domains, PF00954: S-locus
glycoprotein family, PF00069: Protein kinase domain, and
PF01453: Lectin (probable mannose binding)
Length = 840
Score = 27.5 bits (58), Expect = 9.0
Identities = 8/17 (47%), Positives = 15/17 (88%)
Frame = -2
Query: 431 EVSNDRPSKSRILWLIS 381
EV+NDRP+ S ++W+++
Sbjct: 778 EVANDRPNVSNVIWMLT 794
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,224,917
Number of Sequences: 28952
Number of extensions: 365830
Number of successful extensions: 985
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 956
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 983
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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