Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20579
(697 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 128 6e-31
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 128 6e-31
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 70 4e-13
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 69 5e-13
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.9
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 7.8
SPBC19C7.09c |uve1|uvde|endonuclease Uve1 |Schizosaccharomyces p... 25 7.8
SPBC1683.13c |||transcription factor |Schizosaccharomyces pombe|... 25 7.8
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 128 bits (310), Expect = 6e-31
Identities = 54/93 (58%), Positives = 72/93 (77%)
Frame = +1
Query: 256 VVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQ 435
V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQ
Sbjct: 78 VIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQ 137
Query: 436 GFLIFHXXXXXXXXXXXXLLMERLSLTTARSLN 534
GFL+FH LL+ERL++ + N
Sbjct: 138 GFLVFHSFGGGTGSGLGALLLERLNMEYGKKSN 170
Score = 116 bits (279), Expect = 3e-27
Identities = 49/62 (79%), Positives = 58/62 (93%)
Frame = +3
Query: 510 VDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLD 689
++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLD
Sbjct: 163 MEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLD 222
Query: 690 IE 695
IE
Sbjct: 223 IE 224
Score = 110 bits (265), Expect = 2e-25
Identities = 54/80 (67%), Positives = 61/80 (76%), Gaps = 5/80 (6%)
Frame = +2
Query: 38 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 202
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 203 TGAGKHVPRAVFVDLEPMLL 262
TG GK VPR+++VDLEP ++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVI 79
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 128 bits (310), Expect = 6e-31
Identities = 56/86 (65%), Positives = 69/86 (80%)
Frame = +1
Query: 256 VVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQ 435
V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQ
Sbjct: 74 VIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQ 133
Query: 436 GFLIFHXXXXXXXXXXXXLLMERLSL 513
GFL+FH LL+ERL++
Sbjct: 134 GFLVFHSFGGGTGSGFGALLLERLAM 159
Score = 118 bits (283), Expect = 1e-27
Identities = 50/75 (66%), Positives = 60/75 (80%)
Frame = +2
Query: 38 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 217
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 218 HVPRAVFVDLEPMLL 262
+VPR+++VDLEP ++
Sbjct: 61 YVPRSIYVDLEPNVI 75
Score = 111 bits (266), Expect = 1e-25
Identities = 47/62 (75%), Positives = 57/62 (91%)
Frame = +3
Query: 510 VDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLD 689
++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLD
Sbjct: 159 MEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLD 218
Query: 690 IE 695
IE
Sbjct: 219 IE 220
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 69.7 bits (163), Expect = 4e-13
Identities = 31/83 (37%), Positives = 48/83 (57%)
Frame = +1
Query: 259 VDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQG 438
+D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQG
Sbjct: 73 MDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQG 132
Query: 439 FLIFHXXXXXXXXXXXXLLMERL 507
F + H LL+ ++
Sbjct: 133 FQLTHSLGGGTGSGMGTLLLSKI 155
Score = 60.1 bits (139), Expect = 3e-10
Identities = 30/72 (41%), Positives = 38/72 (52%)
Frame = +2
Query: 38 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 217
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 218 HVPRAVFVDLEP 253
+VPRAV VDLEP
Sbjct: 59 YVPRAVLVDLEP 70
Score = 54.8 bits (126), Expect = 1e-08
Identities = 25/61 (40%), Positives = 37/61 (60%)
Frame = +3
Query: 513 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 692
+Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I L I
Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKI 217
Query: 693 E 695
+
Sbjct: 218 K 218
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 69.3 bits (162), Expect = 5e-13
Identities = 34/95 (35%), Positives = 54/95 (56%)
Frame = +2
Query: 41 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 220
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 221 VPRAVFVDLEPMLLMRSALAHTDSCFIQNNLLLVR 325
+PRA+ +DLEP ++ S + N+L+ +
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITK 95
Score = 47.6 bits (108), Expect = 2e-06
Identities = 27/87 (31%), Positives = 45/87 (51%), Gaps = 2/87 (2%)
Frame = +1
Query: 256 VVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVLDRIRKLADQCTG 429
VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D I + AD
Sbjct: 73 VVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDS 131
Query: 430 LQGFLIFHXXXXXXXXXXXXLLMERLS 510
L+GF + H L+ERL+
Sbjct: 132 LEGFSLLHSIAGGTGSGLGSFLLERLN 158
Score = 36.7 bits (81), Expect = 0.003
Identities = 20/58 (34%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
Frame = +3
Query: 516 YGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNL 686
Y KK ++++P Q VS VV+PYNS+L ++D ++DN A+ I L
Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRL 218
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 5.9
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 117 STASSLMARCPQTRPSGVETILSTLSSARPELAST 221
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 136 WPDAHRQDHRGWRRFFQHFLQR 201
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 7.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 27 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 149
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPBC19C7.09c |uve1|uvde|endonuclease Uve1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 599
Score = 25.4 bits (53), Expect = 7.8
Identities = 16/59 (27%), Positives = 26/59 (44%), Gaps = 4/59 (6%)
Frame = +3
Query: 447 LPLLRWRYRLWVHFLIDGASLVDYGKKSK----LEFAIYPAPQVSTAVVEPYNSILTTH 611
+ L+ W + +HF+ + L + +K LEFA +V + YN LT H
Sbjct: 297 IKLVEWNHNFGIHFMRVSSDLFPFASHAKYGYTLEFAQSHLEEVG-KLANKYNHRLTMH 354
>SPBC1683.13c |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 618
Score = 25.4 bits (53), Expect = 7.8
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -3
Query: 248 QDRRRQHGVRACQLRSR*RKC*KNRLHPRWSCL 150
QD++ Q R R R RK +RLHP + C+
Sbjct: 7 QDKKNQEIFRISCQRCRQRKIKCDRLHPCFQCV 39
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,907,702
Number of Sequences: 5004
Number of extensions: 61412
Number of successful extensions: 194
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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