BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20578
(703 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY568009-1|AAS73299.1| 300|Apis mellifera ADP/ATP translocase p... 190 1e-50
AY332626-1|AAQ24500.1| 300|Apis mellifera ADP/ATP translocase p... 190 1e-50
DQ026037-1|AAY87896.1| 431|Apis mellifera nicotinic acetylcholi... 23 2.8
>AY568009-1|AAS73299.1| 300|Apis mellifera ADP/ATP translocase
protein.
Length = 300
Score = 190 bits (463), Expect = 1e-50
Identities = 95/151 (62%), Positives = 113/151 (74%), Gaps = 2/151 (1%)
Frame = +2
Query: 257 SAAVSKTAVAPIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNFAN 436
+AA+SKT VAPIERVKLLLQVQH+SKQI+ +QRYKG++D FVRIPKEQG LS+WRGN AN
Sbjct: 20 AAAISKTTVAPIERVKLLLQVQHISKQISEEQRYKGMIDCFVRIPKEQGFLSYWRGNLAN 79
Query: 437 VIRYFPTQALNFAFKDKYKQVFLGVLTRRRSSGVTSLVIWXXXXXXXXXLCASC--TPST 610
VIRYFPTQALNFAFKDKYKQVFLG + +++ + + C P
Sbjct: 80 VIRYFPTQALNFAFKDKYKQVFLGGVD--KNTQFLRYFVGNLASGGAAGATSLCFVYPLD 137
Query: 611 SHVTRLAADVGKGDGQREFSGLGNCISKIFK 703
TRLAADVGK G+REF+GLGNC++KIFK
Sbjct: 138 FARTRLAADVGKAGGEREFTGLGNCLTKIFK 168
Score = 44.8 bits (101), Expect = 8e-07
Identities = 21/35 (60%), Positives = 21/35 (60%)
Frame = +1
Query: 511 VDKKTQFWRYFXXXXXXXXXXXXTSLCFVYPLDFA 615
VDK TQF RYF TSLCFVYPLDFA
Sbjct: 105 VDKNTQFLRYFVGNLASGGAAGATSLCFVYPLDFA 139
Score = 38.3 bits (85), Expect = 7e-05
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 201 MSNLADPVAFAKDFLAGG 254
MS LADPVAFAKDFLAGG
Sbjct: 1 MSGLADPVAFAKDFLAGG 18
Score = 27.5 bits (58), Expect = 0.13
Identities = 14/53 (26%), Positives = 30/53 (56%)
Frame = +2
Query: 287 PIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNFANVIR 445
P + V+ + +Q S + ++ YK + + I K +G +F++G F+N++R
Sbjct: 232 PFDTVRRRMMMQ--SGRAKSEILYKSTLHCWATIYKTEGGNAFFKGAFSNILR 282
Score = 22.2 bits (45), Expect = 4.9
Identities = 18/76 (23%), Positives = 32/76 (42%)
Frame = +2
Query: 257 SAAVSKTAVAPIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNFAN 436
+ A S V P++ + L V K ++ + G+ + +I K G+ +RG +
Sbjct: 125 AGATSLCFVYPLDFARTRLAAD-VGKA-GGEREFTGLGNCLTKIFKADGITGLYRGFGVS 182
Query: 437 VIRYFPTQALNFAFKD 484
V +A F F D
Sbjct: 183 VQGIIIYRAAYFGFYD 198
>AY332626-1|AAQ24500.1| 300|Apis mellifera ADP/ATP translocase
protein.
Length = 300
Score = 190 bits (463), Expect = 1e-50
Identities = 95/151 (62%), Positives = 113/151 (74%), Gaps = 2/151 (1%)
Frame = +2
Query: 257 SAAVSKTAVAPIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNFAN 436
+AA+SKT VAPIERVKLLLQVQH+SKQI+ +QRYKG++D FVRIPKEQG LS+WRGN AN
Sbjct: 20 AAAISKTTVAPIERVKLLLQVQHISKQISEEQRYKGMIDCFVRIPKEQGFLSYWRGNLAN 79
Query: 437 VIRYFPTQALNFAFKDKYKQVFLGVLTRRRSSGVTSLVIWXXXXXXXXXLCASC--TPST 610
VIRYFPTQALNFAFKDKYKQVFLG + +++ + + C P
Sbjct: 80 VIRYFPTQALNFAFKDKYKQVFLGGVD--KNTQFLRYFVGNLASGGAAGATSLCFVYPLD 137
Query: 611 SHVTRLAADVGKGDGQREFSGLGNCISKIFK 703
TRLAADVGK G+REF+GLGNC++KIFK
Sbjct: 138 FARTRLAADVGKAGGEREFTGLGNCLTKIFK 168
Score = 44.8 bits (101), Expect = 8e-07
Identities = 21/35 (60%), Positives = 21/35 (60%)
Frame = +1
Query: 511 VDKKTQFWRYFXXXXXXXXXXXXTSLCFVYPLDFA 615
VDK TQF RYF TSLCFVYPLDFA
Sbjct: 105 VDKNTQFLRYFVGNLASGGAAGATSLCFVYPLDFA 139
Score = 38.3 bits (85), Expect = 7e-05
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 201 MSNLADPVAFAKDFLAGG 254
MS LADPVAFAKDFLAGG
Sbjct: 1 MSGLADPVAFAKDFLAGG 18
Score = 27.5 bits (58), Expect = 0.13
Identities = 14/53 (26%), Positives = 30/53 (56%)
Frame = +2
Query: 287 PIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNFANVIR 445
P + V+ + +Q S + ++ YK + + I K +G +F++G F+N++R
Sbjct: 232 PFDTVRRRMMMQ--SGRAKSEILYKSTLHCWATIYKTEGGNAFFKGAFSNILR 282
Score = 22.2 bits (45), Expect = 4.9
Identities = 18/76 (23%), Positives = 32/76 (42%)
Frame = +2
Query: 257 SAAVSKTAVAPIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNFAN 436
+ A S V P++ + L V K ++ + G+ + +I K G+ +RG +
Sbjct: 125 AGATSLCFVYPLDFARTRLAAD-VGKA-GGEREFTGLGNCLTKIFKADGITGLYRGFGVS 182
Query: 437 VIRYFPTQALNFAFKD 484
V +A F F D
Sbjct: 183 VQGIIIYRAAYFGFYD 198
>DQ026037-1|AAY87896.1| 431|Apis mellifera nicotinic acetylcholine
receptor alpha9subunit protein.
Length = 431
Score = 23.0 bits (47), Expect = 2.8
Identities = 8/15 (53%), Positives = 9/15 (60%)
Frame = +2
Query: 593 SCTPSTSHVTRLAAD 637
SC PS HV + A D
Sbjct: 158 SCVPSVKHVAKCATD 172
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 188,905
Number of Sequences: 438
Number of extensions: 3850
Number of successful extensions: 17
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 21561255
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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