Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20576
(718 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31450.1 68415.m03842 endonuclease-related similar to endonuc... 110 1e-24
At1g05900.2 68414.m00619 endonuclease-related similar to endonuc... 109 1e-24
At1g05900.1 68414.m00618 endonuclease-related similar to endonuc... 80 1e-15
At3g10010.1 68416.m01201 HhH-GPD base excision DNA repair family... 40 0.001
At3g47830.1 68416.m05212 HhH-GPD base excision DNA repair protei... 36 0.027
At2g36490.1 68415.m04479 HhH-GPD base excision DNA repair family... 33 0.25
At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEME... 31 0.76
At1g52420.1 68414.m05917 glycosyl transferase family 1 protein c... 31 1.0
At1g35040.1 68414.m04341 hypothetical protein 29 2.3
At1g63130.1 68414.m07134 pentatricopeptide (PPR) repeat-containi... 28 5.4
At4g34060.1 68417.m04833 expressed protein similar to DEMETER pr... 28 7.1
At1g34910.1 68414.m04338 hypothetical protein 27 9.4
>At2g31450.1 68415.m03842 endonuclease-related similar to
endonuclease III [Homo sapiens] GI:1753174; contains
Pfam profile PF00633: Helix-hairpin-helix motif
Length = 379
Score = 110 bits (264), Expect = 1e-24
Identities = 48/73 (65%), Positives = 55/73 (75%), Gaps = 5/73 (6%)
Frame = +1
Query: 508 VTGIGVDTHVHRICNRIGWVKKP-----TKTPEETRLALQSWLPFEQWSEVNPLLVGFGQ 672
V GI VDTHVHRICNR+GWV +P T +PEETR+ALQ WLP E+W +NPLLVGFGQ
Sbjct: 278 VQGICVDTHVHRICNRLGWVSRPGTKQKTTSPEETRVALQQWLPKEEWVAINPLLVGFGQ 337
Query: 673 TICQPIKPNCGEC 711
IC PI+P C C
Sbjct: 338 MICTPIRPRCEAC 350
Score = 81.8 bits (193), Expect = 4e-16
Identities = 37/83 (44%), Positives = 52/83 (62%), Gaps = 1/83 (1%)
Frame = +2
Query: 260 AMQRLRARGLTVDNVLSMSDEE-LGQLIYPVGFWKTKVKYIKKTTGILKEQYNGDIPDSV 436
A+ RL GL + +DE + +LIYPVGF+ K Y+KK I +Y+GDIP S+
Sbjct: 194 AIHRLHQNGLLTPEAVDKADESTIKELIYPVGFYTRKATYMKKIARICLVKYDGDIPSSL 253
Query: 437 EKLCKLNGVGPKMAHICMKVAWN 505
+ L L G+GPKMAH+ + +AWN
Sbjct: 254 DDLLSLPGIGPKMAHLILHIAWN 276
Score = 28.3 bits (60), Expect = 5.4
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = +3
Query: 198 RYQCLVSLMLSSQTKDQVN 254
R+ L+ +LSSQTKDQVN
Sbjct: 173 RFAVLLGALLSSQTKDQVN 191
>At1g05900.2 68414.m00619 endonuclease-related similar to
endonuclease III [Homo sapiens] GI:1753174; contains
Pfam profile PF00633: Helix-hairpin-helix motif
Length = 386
Score = 109 bits (263), Expect = 1e-24
Identities = 48/73 (65%), Positives = 56/73 (76%), Gaps = 5/73 (6%)
Frame = +1
Query: 508 VTGIGVDTHVHRICNRIGWVKKP-----TKTPEETRLALQSWLPFEQWSEVNPLLVGFGQ 672
V GI VDTHVHRICNR+GWV KP T +PEETR+ALQ WLP +W +N LLVGFGQ
Sbjct: 285 VQGICVDTHVHRICNRLGWVSKPGTKQKTSSPEETRVALQQWLPKGEWVAINFLLVGFGQ 344
Query: 673 TICQPIKPNCGEC 711
TIC P++P+CG C
Sbjct: 345 TICTPLRPHCGTC 357
Score = 80.2 bits (189), Expect = 1e-15
Identities = 37/83 (44%), Positives = 53/83 (63%), Gaps = 1/83 (1%)
Frame = +2
Query: 260 AMQRLRARGLTVDNVLSMSDEE-LGQLIYPVGFWKTKVKYIKKTTGILKEQYNGDIPDSV 436
A++RL GL + +DE + +LIYPVGF+ K +KK I +Y+GDIP ++
Sbjct: 201 AVERLHQNGLLTPEAIDKADESTIKELIYPVGFYTRKATNVKKVAKICLMEYDGDIPRTL 260
Query: 437 EKLCKLNGVGPKMAHICMKVAWN 505
E+L L GVGPK+AH+ + VAWN
Sbjct: 261 EELLSLPGVGPKIAHLVLHVAWN 283
>At1g05900.1 68414.m00618 endonuclease-related similar to
endonuclease III [Homo sapiens] GI:1753174; contains
Pfam profile PF00633: Helix-hairpin-helix motif
Length = 314
Score = 80.2 bits (189), Expect = 1e-15
Identities = 37/83 (44%), Positives = 53/83 (63%), Gaps = 1/83 (1%)
Frame = +2
Query: 260 AMQRLRARGLTVDNVLSMSDEE-LGQLIYPVGFWKTKVKYIKKTTGILKEQYNGDIPDSV 436
A++RL GL + +DE + +LIYPVGF+ K +KK I +Y+GDIP ++
Sbjct: 201 AVERLHQNGLLTPEAIDKADESTIKELIYPVGFYTRKATNVKKVAKICLMEYDGDIPRTL 260
Query: 437 EKLCKLNGVGPKMAHICMKVAWN 505
E+L L GVGPK+AH+ + VAWN
Sbjct: 261 EELLSLPGVGPKIAHLVLHVAWN 283
Score = 48.0 bits (109), Expect = 6e-06
Identities = 19/23 (82%), Positives = 20/23 (86%)
Frame = +1
Query: 508 VTGIGVDTHVHRICNRIGWVKKP 576
V GI VDTHVHRICNR+GWV KP
Sbjct: 285 VQGICVDTHVHRICNRLGWVSKP 307
>At3g10010.1 68416.m01201 HhH-GPD base excision DNA repair family
protein similar to DEMETER protein [Arabidopsis thaliana]
GI:21743571; contains Pfam profile PF00730: HhH-GPD
superfamily base excision DNA repair protein
Length = 1309
Score = 40.3 bits (90), Expect = 0.001
Identities = 22/63 (34%), Positives = 34/63 (53%)
Frame = +1
Query: 523 VDTHVHRICNRIGWVKKPTKTPEETRLALQSWLPFEQWSEVNPLLVGFGQTICQPIKPNC 702
VDT+V RI R+GWV P+E ++ L + E++ ++ FG+ C +KPNC
Sbjct: 902 VDTNVGRIAVRLGWVPLQP-LPDELQMHLL------ELYELHYHMITFGKVFCTKVKPNC 954
Query: 703 GEC 711
C
Sbjct: 955 NAC 957
>At3g47830.1 68416.m05212 HhH-GPD base excision DNA repair
protein-related
Length = 293
Score = 35.9 bits (79), Expect = 0.027
Identities = 23/59 (38%), Positives = 30/59 (50%)
Frame = +1
Query: 523 VDTHVHRICNRIGWVKKPTKTPEETRLALQSWLPFEQWSEVNPLLVGFGQTICQPIKPN 699
VDTHV I +GWV K T +T + L +P E ++N LL G+ IC K N
Sbjct: 213 VDTHVFEIAKALGWVPK-TADRNKTYVHLNRKIPDELKFDLNCLLYTHGK-ICSNCKKN 269
>At2g36490.1 68415.m04479 HhH-GPD base excision DNA repair family
protein (ROS1) similar to DEMETER protein [Arabidopsis
thaliana] GI:21743571; contains Pfam profile PF00730:
HhH-GPD superfamily base excision DNA repair protein
Length = 1393
Score = 32.7 bits (71), Expect = 0.25
Identities = 25/80 (31%), Positives = 34/80 (42%), Gaps = 17/80 (21%)
Frame = +1
Query: 523 VDTHVHRICNRIGWVKKPTKTPEETRLALQSWLPFEQ------WS-----------EVNP 651
VDT+V RI R+GWV PE +L L P + W E++
Sbjct: 970 VDTNVGRIAVRLGWVPLQP-LPESLQLHLLEMYPMLESIQKYLWPRLCKLDQKTLYELHY 1028
Query: 652 LLVGFGQTICQPIKPNCGEC 711
++ FG+ C KPNC C
Sbjct: 1029 QMITFGKVFCTKSKPNCNAC 1048
>At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEMETER
protein [Arabidopsis thaliana] GI:21743571; contains Pfam
profile PF00730: HhH-GPD superfamily base excision DNA
repair protein
Length = 1729
Score = 31.1 bits (67), Expect = 0.76
Identities = 25/80 (31%), Positives = 34/80 (42%), Gaps = 17/80 (21%)
Frame = +1
Query: 523 VDTHVHRICNRIGWVKKPTKTPEETRLALQSWLPFEQ------WS-----------EVNP 651
VDT+V RI R+GWV PE +L L P + W E++
Sbjct: 1303 VDTNVGRIAVRMGWVPLQP-LPESLQLHLLELYPVLESIQKFLWPRLCKLDQRTLYELHY 1361
Query: 652 LLVGFGQTICQPIKPNCGEC 711
L+ FG+ C +PNC C
Sbjct: 1362 QLITFGKVFCTKSRPNCNAC 1381
>At1g52420.1 68414.m05917 glycosyl transferase family 1 protein
contains Pfam profile: PF00534 Glycosyl transferases
group 1
Length = 670
Score = 30.7 bits (66), Expect = 1.0
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +2
Query: 266 QRLRARGLTVDNVLSMSDEELGQLIYPVGFWKTKVKYIKKTTGILKEQYN 415
+++++ LT+DN L +EL L+ VG KV Y+K+ L N
Sbjct: 493 RQMKSVSLTLDNGLRREKQELKVLLGSVGSKSNKVGYVKEMLSFLSNSGN 542
>At1g35040.1 68414.m04341 hypothetical protein
Length = 106
Score = 29.5 bits (63), Expect = 2.3
Identities = 12/21 (57%), Positives = 16/21 (76%), Gaps = 1/21 (4%)
Frame = +1
Query: 610 QSWLPFEQWSEV-NPLLVGFG 669
QSW+PF WS+V + LL+ FG
Sbjct: 80 QSWIPFNSWSQVKDGLLLTFG 100
>At1g63130.1 68414.m07134 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 630
Score = 28.3 bits (60), Expect = 5.4
Identities = 18/60 (30%), Positives = 26/60 (43%)
Frame = -3
Query: 716 FRHSPQLGFIG*HIVWPNPTSRGLTSLHCSKGNQLCRARRVSSGVLVGFLTHPILLHILC 537
FR Q G +G + + + C Q+ + VS GVL +T+ ILL LC
Sbjct: 419 FREMSQRGLVGNTVTYTTLIHGFFQARECDNA-QIVFKQMVSDGVLPDIMTYSILLDGLC 477
>At4g34060.1 68417.m04833 expressed protein similar to DEMETER
protein [Arabidopsis thaliana] GI:21743571; expression
supported by MPSS
Length = 1073
Score = 27.9 bits (59), Expect = 7.1
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = +1
Query: 619 LPFEQWSEVNPLLVGFGQTICQPIKPNCGEC 711
LP E E++ ++ FG+ C PNC C
Sbjct: 687 LPQETLYELHYQMITFGKVFCTKTIPNCNAC 717
>At1g34910.1 68414.m04338 hypothetical protein
Length = 136
Score = 27.5 bits (58), Expect = 9.4
Identities = 11/20 (55%), Positives = 15/20 (75%), Gaps = 1/20 (5%)
Frame = +1
Query: 613 SWLPFEQWSEV-NPLLVGFG 669
SW+PF WS+V + LL+ FG
Sbjct: 82 SWIPFNSWSQVKDGLLLTFG 101
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,483,278
Number of Sequences: 28952
Number of extensions: 330270
Number of successful extensions: 922
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 875
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 919
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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