BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20575 (719 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g40820.1 68418.m04956 FAT domain-containing protein / phospha... 30 1.8 At1g13330.1 68414.m01547 expressed protein similar to nuclear re... 29 2.3 At1g70530.1 68414.m08117 protein kinase family protein contains ... 29 4.1 At4g15890.1 68417.m02415 expressed protein 28 5.4 At4g14310.2 68417.m02205 peroxisomal membrane protein-related co... 28 7.2 At2g18630.1 68415.m02169 expressed protein unusual splice site a... 28 7.2 At3g10690.1 68416.m01286 DNA gyrase subunit A family protein sim... 27 9.5 At1g64960.1 68414.m07363 expressed protein 27 9.5 At1g61320.1 68414.m06911 hypothetical protein 27 9.5 >At5g40820.1 68418.m04956 FAT domain-containing protein / phosphatidylinositol 3- and 4-kinase family protein similar to Atr protein [Xenopus laevis] GI:11385422; contains Pfam profiles PF00454 Phosphatidylinositol 3- and 4-kinase, PF02259 FAT domain, PF02260 FAT C domain Length = 2702 Score = 29.9 bits (64), Expect = 1.8 Identities = 19/66 (28%), Positives = 33/66 (50%) Frame = +3 Query: 33 LIMHKIKFELPDNSAESTKYLITLLIKEIHPSIGEIRKKLQVKHIEEQELIKIREVIALC 212 L++H +L D S S KY+I+ + + P + K+ + H+ E++KI E + L Sbjct: 1160 LVLHFFTRKLADVSPSSIKYVISQIFAALIPF---LEKEKEGPHVYLDEVVKILEELVLK 1216 Query: 213 CNQIKK 230 I K Sbjct: 1217 NRDIVK 1222 >At1g13330.1 68414.m01547 expressed protein similar to nuclear receptor coactivator GT198 (GI:16506273) {Rattus norvegicus}; similar to TBP-1 interacting protein (GI:7328534) [Homo sapiens] Length = 226 Score = 29.5 bits (63), Expect = 2.3 Identities = 21/65 (32%), Positives = 35/65 (53%) Frame = +3 Query: 51 KFELPDNSAESTKYLITLLIKEIHPSIGEIRKKLQVKHIEEQELIKIREVIALCCNQIKK 230 K + D +E L ++EI ++RK +VK +EE+ L+K+RE I L + KK Sbjct: 99 KKTISDVESEIKSLQSNLTLEEIQEKDAKLRK--EVKEMEEK-LVKLREGITLVRPEDKK 155 Query: 231 CVVNL 245 V ++ Sbjct: 156 AVEDM 160 >At1g70530.1 68414.m08117 protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain Length = 646 Score = 28.7 bits (61), Expect = 4.1 Identities = 17/52 (32%), Positives = 27/52 (51%) Frame = +2 Query: 437 WIDHTFEVLSKLSEVVYKNDYKENLGCVSSGNLNCCRY*AIFILQLMSYCYL 592 W+DH F ++ +S+V +KN K LGC +G + Y I L Y ++ Sbjct: 360 WVDHFFNEVNLISQVDHKNLVK-LLGCSITGPESLLVYEYIANQSLHDYLFV 410 >At4g15890.1 68417.m02415 expressed protein Length = 1314 Score = 28.3 bits (60), Expect = 5.4 Identities = 15/62 (24%), Positives = 33/62 (53%) Frame = +1 Query: 520 ELWKSELLQILSDLHLAIDELLLSAMTLCKYCLPSDQHIVKARCQVVLRETKALMTELIS 699 EL KS+ + ++L +A+ + + + + +P ++ C+VV R+T L++ L+ Sbjct: 891 ELEKSDCAALRNNLVVAMTDFCVHYTAMIECYIPKITKRLRDPCEVVRRQTFILLSRLLQ 950 Query: 700 GD 705 D Sbjct: 951 RD 952 >At4g14310.2 68417.m02205 peroxisomal membrane protein-related contains weak similarity to Peroxisomal membrane protein 2 (22 kDa peroxisomal membrane protein) (Swiss-Prot:P42925) [Mus musculus] Length = 965 Score = 27.9 bits (59), Expect = 7.2 Identities = 8/38 (21%), Positives = 20/38 (52%) Frame = -2 Query: 211 HSAITSRILINSCSSMCLTCNFFRISPILGCISLIKSV 98 + S ++N C+S+ + + ++ + G + +KSV Sbjct: 546 YDVANSEFMVNECNSLSIWVRLYEVTGVFGFVHYVKSV 583 >At2g18630.1 68415.m02169 expressed protein unusual splice site at second intron; GA instead of conserved GT at donor site; similar to At14a GI:11994571 and GI:11994573 [Arabidopsis thaliana] Length = 393 Score = 27.9 bits (59), Expect = 7.2 Identities = 16/49 (32%), Positives = 30/49 (61%) Frame = +3 Query: 12 KEFRNNELIMHKIKFELPDNSAESTKYLITLLIKEIHPSIGEIRKKLQV 158 KE N +++ K++ E+ ++ + ++ IT KE+ +I EI+KKL V Sbjct: 299 KEMDNISILVRKVEVEI-ESLLKKAEFAITEE-KEVRLAIDEIKKKLDV 345 >At3g10690.1 68416.m01286 DNA gyrase subunit A family protein similar to SP|P94605 DNA gyrase subunit A (EC 5.99.1.3). {Clostridium acetobutylicum}; contains Pfam profiles PF00521: DNA gyrase/topoisomerase IV A subunit, PF03989: DNA gyrase C-terminal domain beta-propeller Length = 950 Score = 27.5 bits (58), Expect = 9.5 Identities = 13/37 (35%), Positives = 18/37 (48%) Frame = -1 Query: 398 LYCH*TNYCFFFQDVGLLYLICLCTIPISLRDIGGFP 288 L CH ++ FF D G++Y IP R+ G P Sbjct: 644 LVCHAHDHVLFFSDRGIVYSTRAYKIPECSRNAAGTP 680 >At1g64960.1 68414.m07363 expressed protein Length = 1168 Score = 27.5 bits (58), Expect = 9.5 Identities = 9/27 (33%), Positives = 19/27 (70%) Frame = -2 Query: 130 ILGCISLIKSVIKYFVDSAELSGSSNF 50 +L + ++ ++ K+FV+S E+ +SNF Sbjct: 891 VLNKVKMLTAIFKFFVESTEMGLASNF 917 >At1g61320.1 68414.m06911 hypothetical protein Length = 459 Score = 27.5 bits (58), Expect = 9.5 Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 1/49 (2%) Frame = +3 Query: 51 KFELPDNSAESTKYLITLLIKEIHPSIGEIRKKL-QVKHIEEQELIKIR 194 KFE+PD+S + K L TL + + I H+E ELI+ R Sbjct: 158 KFEIPDSSPKGLKLLKTLSLMRTQVMVKTIDAIFNNCIHLESLELIECR 206 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,013,290 Number of Sequences: 28952 Number of extensions: 267749 Number of successful extensions: 675 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 668 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 675 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1565336320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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