BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20570 (724 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 123 3e-29 SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 119 4e-28 SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 27 2.7 SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 3.6 SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pom... 27 3.6 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 27 3.6 SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 4.7 SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 26 4.7 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 26 4.7 SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 26 4.7 SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 26 6.3 SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 26 6.3 SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regula... 25 8.3 SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 25 8.3 SPBC17G9.11c |pyr1||pyruvate carboxylase|Schizosaccharomyces pom... 25 8.3 SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 8.3 SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr ... 25 8.3 SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 8.3 >SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 123 bits (296), Expect = 3e-29 Identities = 58/86 (67%), Positives = 71/86 (82%), Gaps = 2/86 (2%) Frame = +1 Query: 256 ERNLLSVAYKNVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDV 429 ERNLLSVAYKN++GARR+SWR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DV Sbjct: 42 ERNLLSVAYKNIIGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDV 101 Query: 430 LGLLDKHLIPKASNPESKVFYLKMKG 507 L +L+KHLIP A+ ESKVFY KMKG Sbjct: 102 LSVLEKHLIPAATTGESKVFYYKMKG 127 Score = 91.5 bits (217), Expect = 1e-19 Identities = 42/70 (60%), Positives = 52/70 (74%) Frame = +3 Query: 510 YYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNS 689 YYRYLAE GE + S +AY+ A +I+ A++ PT P+RLGLALNFSVFYYEIL+S Sbjct: 129 YYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDS 188 Query: 690 PDKACQLAKQ 719 P+ AC LAKQ Sbjct: 189 PESACHLAKQ 198 Score = 37.9 bits (84), Expect = 0.001 Identities = 20/39 (51%), Positives = 26/39 (66%) Frame = +2 Query: 134 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELS 250 MS +E V AKLAEQAERY++M MK+V + +LS Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLS 39 >SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 119 bits (287), Expect = 4e-28 Identities = 56/86 (65%), Positives = 71/86 (82%), Gaps = 2/86 (2%) Frame = +1 Query: 256 ERNLLSVAYKNVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDV 429 ERNLLSVAYKNV+GARR+SWR++SSIEQK E G+ + ++ KEYR K+E+EL IC D+ Sbjct: 43 ERNLLSVAYKNVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDI 102 Query: 430 LGLLDKHLIPKASNPESKVFYLKMKG 507 L +L+KHLIP A++ ESKVFY KMKG Sbjct: 103 LTVLEKHLIPNAASAESKVFYYKMKG 128 Score = 105 bits (252), Expect = 6e-24 Identities = 49/71 (69%), Positives = 56/71 (78%) Frame = +3 Query: 510 YYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNS 689 YYRYLAE A GE R + S + Y+ A EI+ A++ PTHPIRLGLALNFSVFYYEILNS Sbjct: 130 YYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNS 189 Query: 690 PDKACQLAKQA 722 PD+AC LAKQA Sbjct: 190 PDRACYLAKQA 200 Score = 39.1 bits (87), Expect = 6e-04 Identities = 19/38 (50%), Positives = 24/38 (63%) Frame = +2 Query: 137 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELS 250 + +E+ V AKLAEQAERY+ M MK V T EL+ Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELT 40 >SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 1207 Score = 27.1 bits (57), Expect = 2.7 Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 2/51 (3%) Frame = +3 Query: 516 RYLAEVATG-ETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 662 RY+ ++ E R ED+++A + ++ + A +QP H I L LA+N S Sbjct: 1049 RYIVALSCELERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099 >SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr 2|||Manual Length = 463 Score = 26.6 bits (56), Expect = 3.6 Identities = 12/39 (30%), Positives = 20/39 (51%) Frame = +1 Query: 283 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 399 KN+ S+ R +SS + K +E + M +Y K+E Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388 >SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 26.6 bits (56), Expect = 3.6 Identities = 25/91 (27%), Positives = 41/91 (45%), Gaps = 9/91 (9%) Frame = +1 Query: 316 RVISSIEQKTEGSERKQQMAKEYRVKVEKE-LREICYDVLGL--LDKHL---IPKASNPE 477 +V+SS + E S + + R E E L E+ +V L +D+ +P+ NP Sbjct: 263 KVLSSSSEDDESSSPEDLLKPIIRSTEEMENLNELEQEVQDLDPIDEGFEEKVPRFRNPS 322 Query: 478 SKVFYLKMKGV---TTGTLQKWPQEKPDILL 561 K FY K+ + + L+ EK D L+ Sbjct: 323 KKAFYEKLHSLRNRSYSKLESLTSEKSDTLI 353 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 26.6 bits (56), Expect = 3.6 Identities = 16/62 (25%), Positives = 26/62 (41%) Frame = +3 Query: 486 VLPQNERSYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSV 665 +LP NE Y+ + +G S SQ+ D +EI K + I + +SV Sbjct: 187 LLPLNEYPEYKLFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSV 246 Query: 666 FY 671 + Sbjct: 247 IF 248 >SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 26.2 bits (55), Expect = 4.7 Identities = 16/41 (39%), Positives = 23/41 (56%) Frame = -2 Query: 261 PLPLLSSTPVSVTSFIAAAMSSYRSACSANLARCTSSSLST 139 PL S T + +SFI + +SS S++ A TSSS +T Sbjct: 65 PLSSSSFTSPASSSFITSLVSSSSQQSSSSSASLTSSSSAT 105 Score = 25.8 bits (54), Expect = 6.3 Identities = 19/56 (33%), Positives = 31/56 (55%), Gaps = 1/56 (1%) Frame = -2 Query: 306 PSGTYDILISN*KEVPLPLLSSTPVSVTSFIAAAMSSY-RSACSANLARCTSSSLS 142 PS + +L S+ SS+P+S +SF + A SS+ S S++ + +SSS S Sbjct: 42 PSSSSSMLSSSSATAISSSSSSSPLSSSSFTSPASSSFITSLVSSSSQQSSSSSAS 97 >SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 26.2 bits (55), Expect = 4.7 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = +1 Query: 337 QKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 462 +K + + KEY K +++LR+ D+ LL KH I + Sbjct: 328 EKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 26.2 bits (55), Expect = 4.7 Identities = 19/64 (29%), Positives = 30/64 (46%), Gaps = 1/64 (1%) Frame = +1 Query: 340 KTEGSERKQQMAKEYRVKVEKELREI-CYDVLGLLDKHLIPKASNPESKVFYLKMKGVTT 516 K +G RK ++ R E LR C+ + +L + PES+ +YL++K Sbjct: 1476 KLQGRARKTELHGFIRANAEMVLRSPECF--IKILKDCCVLGHFTPESEHYYLELKESLP 1533 Query: 517 GTLQ 528 G LQ Sbjct: 1534 GVLQ 1537 >SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 1462 Score = 26.2 bits (55), Expect = 4.7 Identities = 12/32 (37%), Positives = 14/32 (43%) Frame = -1 Query: 166 TLHQFFLVDGHRGRWKRTNSLMPELLKPAVLE 71 TL FL DG RWK + P V+E Sbjct: 52 TLKDEFLADGLESRWKTNGEKSSSIRYPLVME 83 >SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 487 Score = 25.8 bits (54), Expect = 6.3 Identities = 12/45 (26%), Positives = 22/45 (48%) Frame = +1 Query: 271 SVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 405 S +Y + G S W+ I ++ K+ G +R ++ Y +KE Sbjct: 61 SFSYPFLKGKSDSPWQAIQLLDFKSSGQQRAAYYSERYHSFRDKE 105 >SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 25.8 bits (54), Expect = 6.3 Identities = 14/51 (27%), Positives = 23/51 (45%) Frame = +3 Query: 45 EIKKK*CLHSKTAGFNNSGISELVLFHRPRCPSTRKNWCNVPNWPNKLSDM 197 ++K+ + S F N S+ + F +PR K N WPN+ S + Sbjct: 50 QLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETSHL 100 >SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regulator Prp45|Schizosaccharomyces pombe|chr 3|||Manual Length = 557 Score = 25.4 bits (53), Expect = 8.3 Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 3/50 (6%) Frame = +1 Query: 271 SVAYKNVVGARRSSWRVISSIEQKTEGSE---RKQQMAKEYRVKVEKELR 411 SV+ + +R S+ S E + E SE R+Q++ +E R + EK+LR Sbjct: 358 SVSSEERSRSRAGSFSHHSESENEDEDSEAFRRRQELRRERRRQAEKDLR 407 >SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizosaccharomyces pombe|chr 2|||Manual Length = 1706 Score = 25.4 bits (53), Expect = 8.3 Identities = 10/21 (47%), Positives = 12/21 (57%) Frame = -2 Query: 549 WFLLWPLLQGTCSNSFHFEVK 487 W LLW L GT S S+H + Sbjct: 1393 WLLLWDLRFGTLSCSWHMPAR 1413 >SPBC17G9.11c |pyr1||pyruvate carboxylase|Schizosaccharomyces pombe|chr 2|||Manual Length = 1185 Score = 25.4 bits (53), Expect = 8.3 Identities = 14/47 (29%), Positives = 23/47 (48%) Frame = +3 Query: 492 PQNERSYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHP 632 P N R+ R + GE RH VED + A + +++ + P +P Sbjct: 1071 PLNPRTGQREVYFELNGENRHVTVEDKKAAIE---TVTRPRADPGNP 1114 >SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 85 Score = 25.4 bits (53), Expect = 8.3 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = -3 Query: 626 CGLHFRLADFKSILVCFL*ILYNRMSGFSCGHFC 525 C L+F L + K+ L+ I+Y + GFS H C Sbjct: 46 CLLNFSLRENKNYLI----IVYLPIEGFSANHMC 75 >SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 25.4 bits (53), Expect = 8.3 Identities = 11/32 (34%), Positives = 16/32 (50%) Frame = +3 Query: 489 LPQNERSYYRYLAEVATGETRHSVVEDSQKAY 584 L + E Y R+L E A + V+ED +Y Sbjct: 242 LEEEEHGYERFLLESAQSKEARKVLEDLSSSY 273 >SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosaccharomyces pombe|chr 2|||Manual Length = 421 Score = 25.4 bits (53), Expect = 8.3 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%) Frame = +1 Query: 355 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 477 E++Q A +YR+KVE+ +I V + L+ L + SNPE Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,079,607 Number of Sequences: 5004 Number of extensions: 63850 Number of successful extensions: 218 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 192 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 216 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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