BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20567 (415 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g19730.1 68415.m02305 60S ribosomal protein L28 (RPL28A) 53 9e-08 At4g29410.1 68417.m04200 60S ribosomal protein L28 (RPL28C) unkn... 50 8e-07 At2g46240.1 68415.m05750 IQ domain-containing protein / BAG doma... 29 0.94 At4g17230.1 68417.m02591 scarecrow-like transcription factor 13 ... 29 1.6 At1g06420.1 68414.m00679 expressed protein ; expression supporte... 29 1.6 At1g69523.1 68414.m07991 UbiE/COQ5 methyltransferase family prot... 28 2.9 At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nic... 27 5.0 At4g38150.1 68417.m05386 pentatricopeptide (PPR) repeat-containi... 27 6.6 At2g29605.1 68415.m03595 hypothetical protein 27 6.6 At3g44730.1 68416.m04814 kinesin motor protein-related similar t... 26 8.7 >At2g19730.1 68415.m02305 60S ribosomal protein L28 (RPL28A) Length = 143 Score = 52.8 bits (121), Expect = 9e-08 Identities = 29/79 (36%), Positives = 45/79 (56%), Gaps = 7/79 (8%) Frame = +2 Query: 2 NNAFLVK---KRNIKKPFSKEPNNVTNLHSFRYNGLIHKKAVGVVENPDRKGFTVVYKKA 172 NN FLVK + N K FSKE NN+TN+HS++++GL +KK V + + + K Sbjct: 16 NNCFLVKQFGRGNSKVQFSKETNNLTNVHSYKHSGLANKKTVTIQAADKDQAVVLATTKT 75 Query: 173 KATRKPA----KNLIRRPF 217 K KP K+++++ F Sbjct: 76 KKQNKPKLSVNKSILKKEF 94 Score = 27.1 bits (57), Expect = 5.0 Identities = 13/23 (56%), Positives = 15/23 (65%) Frame = +1 Query: 268 NHYRTDLCKATLRRASAILRSQR 336 N+YR DL KA L R SAI + R Sbjct: 108 NYYRPDLKKAALARLSAISKGLR 130 >At4g29410.1 68417.m04200 60S ribosomal protein L28 (RPL28C) unknown protein chromosome II BAC F6F22 - Arabidopsis thaliana,PID:g3687251 Length = 143 Score = 49.6 bits (113), Expect = 8e-07 Identities = 28/79 (35%), Positives = 45/79 (56%), Gaps = 7/79 (8%) Frame = +2 Query: 2 NNAFLVK---KRNIKKPFSKEPNNVTNLHSFRYNGLIHKKAVGVVENPDRKGFTVVYKKA 172 NN FLVK + N K FSKE NN+ N++S++++GL +KK V + +G + K Sbjct: 16 NNCFLVKQFGRGNAKVQFSKESNNLVNINSYKHSGLANKKTVTIQAAGKDQGVVLGTTKT 75 Query: 173 KATRKPA----KNLIRRPF 217 K KP K+++++ F Sbjct: 76 KRQNKPKLSVNKSILKKEF 94 Score = 27.1 bits (57), Expect = 5.0 Identities = 13/23 (56%), Positives = 15/23 (65%) Frame = +1 Query: 268 NHYRTDLCKATLRRASAILRSQR 336 N+YR DL KA L R SAI + R Sbjct: 108 NYYRPDLKKAALARLSAISKGLR 130 >At2g46240.1 68415.m05750 IQ domain-containing protein / BAG domain-containing protein contains Pfam profiles PF00612: IQ calmodulin-binding motif, PF02179: BAG (Apoptosis regulator Bcl-2 protein) domain Length = 1043 Score = 29.5 bits (63), Expect = 0.94 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = +3 Query: 279 HRPMQGYPSSCFSHPPLPEAHQ 344 H P Y SC+ HPP P +Q Sbjct: 73 HPPHASYAPSCYVHPPFPVGYQ 94 >At4g17230.1 68417.m02591 scarecrow-like transcription factor 13 (SCL13) Length = 287 Score = 28.7 bits (61), Expect = 1.6 Identities = 13/35 (37%), Positives = 21/35 (60%) Frame = +1 Query: 25 AQYQKAVQQGAEQCD*PPLLQVQRLDSQESRWCRG 129 +QY +Q+ A+ PPLL+V +D +S + RG Sbjct: 91 SQYMFLIQELAKHPGGPPLLRVTGVDDSQSTYARG 125 >At1g06420.1 68414.m00679 expressed protein ; expression supported by MPSS Length = 221 Score = 28.7 bits (61), Expect = 1.6 Identities = 16/50 (32%), Positives = 22/50 (44%) Frame = +2 Query: 110 KAVGVVENPDRKGFTVVYKKAKATRKPAKNLIRRPFKAGARRSLYKVKSC 259 K G+V N D G V + K + P K RR FK+ + +V C Sbjct: 110 KVSGIVSNKDEDG---VDSREKKKKNPVKENTRRVFKSKGENAAKEVTQC 156 >At1g69523.1 68414.m07991 UbiE/COQ5 methyltransferase family protein low similarity to SP|Q05197 Phosphatidylethanolamine N-methyltransferase (EC 2.1.1.17) [Rhodopseudomonas sphaeroides] {Rhodobacter sphaeroides}; contains Pfam profile PF01209: methlytransferase, UbiE/COQ5 family Length = 300 Score = 27.9 bits (59), Expect = 2.9 Identities = 14/46 (30%), Positives = 20/46 (43%) Frame = +2 Query: 236 SLYKVKSC*RLTTTAQTYARLPFVVLQPSSAPRGPSKQKRLRQPQP 373 S ++ C R +PF+ + PS A KRLR P+P Sbjct: 43 SSFRFCPCGRRHFIGAAMTSMPFLPISPSHASTSTEDLKRLRPPKP 88 >At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nicotiana tabacum, EMBL:AB009883 Length = 1008 Score = 27.1 bits (57), Expect = 5.0 Identities = 18/57 (31%), Positives = 25/57 (43%) Frame = +2 Query: 20 KKRNIKKPFSKEPNNVTNLHSFRYNGLIHKKAVGVVENPDRKGFTVVYKKAKATRKP 190 K+ + ++ KEP+ T R N KKA V E RK KK + +KP Sbjct: 770 KQSDGEEETQKEPSESTKKERKRKNPESKKKAEAVEEEETRKESVESTKKERKRKKP 826 >At4g38150.1 68417.m05386 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 302 Score = 26.6 bits (56), Expect = 6.6 Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 1/29 (3%) Frame = +3 Query: 270 PLPHRPMQGYPSS-CFSHPPLPEAHQSKK 353 PLP+RP++G SS PP +AH K Sbjct: 48 PLPNRPLRGERSSNSHREPPARQAHNLGK 76 >At2g29605.1 68415.m03595 hypothetical protein Length = 401 Score = 26.6 bits (56), Expect = 6.6 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = -1 Query: 148 SLPVRVLHDTNGFLVNQAVVPEGVEVSHI 62 ++ + L DTNG VNQ +P G + +HI Sbjct: 121 NMGMSALDDTNGLTVNQLPIPNG-QQNHI 148 >At3g44730.1 68416.m04814 kinesin motor protein-related similar to 4 other kinesin-like proteins of A. thaliana: F02P16.12 (PID:g2191180), katA (D11371), katB (D21137), and katC (D21138); contains non-consensus AT-AC splice sites at intron 10 Length = 1087 Score = 26.2 bits (55), Expect = 8.7 Identities = 14/49 (28%), Positives = 24/49 (48%) Frame = +2 Query: 242 YKVKSC*RLTTTAQTYARLPFVVLQPSSAPRGPSKQKRLRQPQPSRNNL 388 Y+ +SC +T Q + P + ++PR P+ + P PSR +L Sbjct: 789 YETRSC---STGKQRKSGFPSALRNREASPRMPNLAEERLNPSPSRRSL 834 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,772,189 Number of Sequences: 28952 Number of extensions: 180558 Number of successful extensions: 467 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 459 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 467 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 625471056 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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