BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20564 (655 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC29A10.03c |rlf2|SPBC365.19c|chromatin remodeling complex sub... 32 0.063 SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces... 30 0.25 SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain pro... 30 0.25 SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 30 0.34 SPAC17G6.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 29 0.44 SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces ... 29 0.44 SPBC13E7.07 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 29 0.59 SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit ... 29 0.77 SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.77 SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 29 0.77 SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 28 1.4 SPACUNK12.02c |cmk1||calcium/calmodulin-dependent protein kinase... 27 1.8 SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 27 1.8 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 27 2.4 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 26 4.1 SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 26 4.1 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 5.5 SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyc... 25 7.2 SPCC188.12 |spn6|SPCC584.09|septin Spn6|Schizosaccharomyces pomb... 25 7.2 SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam... 25 7.2 SPBC577.05c |rec27|mug41|meiotic recombination protein Rec27|Sch... 25 7.2 SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo... 25 9.5 SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 25 9.5 >SPBC29A10.03c |rlf2|SPBC365.19c|chromatin remodeling complex subunit Rlf2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 544 Score = 32.3 bits (70), Expect = 0.063 Identities = 16/74 (21%), Positives = 39/74 (52%) Frame = +1 Query: 67 KKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEKLQQAADRRLLIEAEQREKLRNHNIK 246 +K +++ R EE++R+ A ++ ++ + QEK + ++ + ++ EKL I+ Sbjct: 77 EKKLQKQRAQEERIRQKEAERLKREKERQQREQEKKLREQEKIAAKKMKELEKLEKERIR 136 Query: 247 LAESARPRPLKSRR 288 L E R + + ++ Sbjct: 137 LQEQQRRKEERDQK 150 Score = 31.1 bits (67), Expect = 0.15 Identities = 20/87 (22%), Positives = 44/87 (50%) Frame = +1 Query: 46 DKRDENLKKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEKLQQAADRRLLIEAEQREK 225 +K+ + + ER+R+ E + K Q++ Q+ + QEK+ AA + +E ++E+ Sbjct: 77 EKKLQKQRAQEERIRQKEAERLKREKERQQREQEKKLREQEKI--AAKKMKELEKLEKER 134 Query: 226 LRNHNIKLAESARPRPLKSRRSLKTLR 306 +R + + R + L+ + + LR Sbjct: 135 IRLQEQQRRKEERDQKLREKEEAQRLR 161 Score = 29.5 bits (63), Expect = 0.44 Identities = 26/84 (30%), Positives = 41/84 (48%) Frame = +1 Query: 4 EVYKAIEDKMTTAADKRDENLKKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEKLQQA 183 E K + ++ AA K E K ER+R E+Q RK +E+ Q+L +E+ Q+ Sbjct: 108 EQEKKLREQEKIAAKKMKELEKLEKERIRLQEQQRRK-----EERDQKLRE--KEEAQRL 160 Query: 184 ADRRLLIEAEQREKLRNHNIKLAE 255 ++L + Q+ KL N K E Sbjct: 161 RQEQILNKERQQLKLNNFFTKGVE 184 >SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces pombe|chr 1|||Manual Length = 324 Score = 30.3 bits (65), Expect = 0.25 Identities = 20/84 (23%), Positives = 38/84 (45%), Gaps = 1/84 (1%) Frame = +1 Query: 13 KAIEDKMTTAADKRDENLKKMIERLREHEEQVRKVRAGNQEKFQQ-LESAIQEKLQQAAD 189 K ++ ++R +KKM E+ E++ ++ ++KF + LE A + AD Sbjct: 134 KKSRNRQKERLERRKAEMKKMSEQAELESEKMADLKNEEKKKFSKILEEAGLVAVDIPAD 193 Query: 190 RRLLIEAEQREKLRNHNIKLAESA 261 L + + +HN+KL A Sbjct: 194 GNCLFASISHQLNYHHNVKLNSQA 217 Score = 27.9 bits (59), Expect = 1.4 Identities = 16/60 (26%), Positives = 31/60 (51%) Frame = +1 Query: 49 KRDENLKKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEKLQQAADRRLLIEAEQREKL 228 K +E L K E +E + ++ +R +E ++ + A+Q+K+ Q A +R+KL Sbjct: 24 KMEELLSKQREECKELQSKITNLRKQLKEGNKKQKRALQQKISQMEADLSQKHATERQKL 83 >SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 240 Score = 30.3 bits (65), Expect = 0.25 Identities = 25/104 (24%), Positives = 50/104 (48%), Gaps = 8/104 (7%) Frame = +1 Query: 22 EDKMTTAADKRDENLKKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEKLQQAADRRLL 201 E + A +++ N K + + E + +RK++ +Q + + L+ Q+K + A R+ + Sbjct: 61 EKERILALEEKKTNYKILQKSNDETAQAMRKMQ--DQARLRDLQKIRQQKAEDAEQRKKI 118 Query: 202 IEAEQREKLR------NHNIKLAESARP--RPLKSRRSLKTLRT 309 + +R+K R N N + E+A P +P + S RT Sbjct: 119 LAEIERDKKRRAAERENKNSSVKETAAPIKQPKNANSSSTCTRT 162 >SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 287 Score = 29.9 bits (64), Expect = 0.34 Identities = 19/48 (39%), Positives = 30/48 (62%), Gaps = 1/48 (2%) Frame = +1 Query: 4 EVYKAIED-KMTTAADKRDENLKKMIERLREHEEQVRKVRAGNQEKFQ 144 E+ KA+E+ K+T DENLKK I+ L E+ +K+ G QE+++ Sbjct: 35 ELVKALENVKLTKFTGDGDENLKKNIKVLVPVNEKPQKL-DGKQEEYE 81 >SPAC17G6.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 29.5 bits (63), Expect = 0.44 Identities = 19/74 (25%), Positives = 37/74 (50%), Gaps = 3/74 (4%) Frame = +1 Query: 52 RDENLKKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEKLQQAADRRL---LIEAEQRE 222 +D +L+ +E L + E +++ + + +E A QE LQQ R+ L E EQ Sbjct: 19 QDHSLQMALEELNQQIEVIQRQEEQLALRKKAIEDARQEVLQQIQHRKFRQYLHEREQEA 78 Query: 223 KLRNHNIKLAESAR 264 +L+ + ++ + R Sbjct: 79 RLQEYYLEQLQERR 92 >SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 29.5 bits (63), Expect = 0.44 Identities = 19/68 (27%), Positives = 37/68 (54%) Frame = +1 Query: 49 KRDENLKKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEKLQQAADRRLLIEAEQREKL 228 ++DE K+ ++ R+ EQV + +EK+ +LE + +LQ A R ++A+ + Sbjct: 436 EKDEASKERLKAARKEAEQVEEETRPIREKY-ELEKSRGSELQDAKRRLDELKAKAEDAE 494 Query: 229 RNHNIKLA 252 R ++ LA Sbjct: 495 RRNDFTLA 502 >SPBC13E7.07 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 29.1 bits (62), Expect = 0.59 Identities = 14/36 (38%), Positives = 22/36 (61%) Frame = +1 Query: 163 QEKLQQAADRRLLIEAEQREKLRNHNIKLAESARPR 270 Q+K +A + + L + EQR +L H +L E+ RPR Sbjct: 207 QQKKLRAKEMQELADEEQRRRLAAHRKELHEANRPR 242 >SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit Bdp1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 507 Score = 28.7 bits (61), Expect = 0.77 Identities = 25/85 (29%), Positives = 47/85 (55%), Gaps = 1/85 (1%) Frame = +1 Query: 22 EDKMTTAADKRDENLKKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEKLQQAADRRLL 201 E++ A + +KK I+ + E+ + KV +E ++L+ I+E ++ +RR Sbjct: 409 EERRNPARVNQALKIKKPID-MEEYSKVSGKVFRPVEEMEKELQK-IRENFEE--ERRRA 464 Query: 202 IE-AEQREKLRNHNIKLAESARPRP 273 IE AEQR+ + NH ++ ++A P P Sbjct: 465 IEVAEQRQLIVNHELEQEKNA-PSP 488 >SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1079 Score = 28.7 bits (61), Expect = 0.77 Identities = 20/105 (19%), Positives = 50/105 (47%), Gaps = 3/105 (2%) Frame = +1 Query: 1 AEVYKAIEDKMTTAADKRDEN---LKKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEK 171 AE + + K + ++ N L+KM+E R EE+ +++R + + + E + E Sbjct: 226 AENDSSAKGKQAAGSKRKGPNVTALQKMLEEKRAREEEEQRIRE-EEARIAEEEKRLAE- 283 Query: 172 LQQAADRRLLIEAEQREKLRNHNIKLAESARPRPLKSRRSLKTLR 306 +++A ++ +++E+ + +K + K +++L R Sbjct: 284 VEEARKEEARLKKKEKERKKKEEMKAQGKYLSKKQKEQQALAQRR 328 >SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 28.7 bits (61), Expect = 0.77 Identities = 21/74 (28%), Positives = 33/74 (44%) Frame = +1 Query: 7 VYKAIEDKMTTAADKRDENLKKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEKLQQAA 186 +Y+A+ +K A K E + K E RE E + + FQ + +EK+ Q Sbjct: 343 IYEALYEKFLKAC-KDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEKIIQEK 401 Query: 187 DRRLLIEAEQREKL 228 R + E R+KL Sbjct: 402 SRVDKMIDEYRQKL 415 >SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 27.9 bits (59), Expect = 1.4 Identities = 13/25 (52%), Positives = 18/25 (72%) Frame = +1 Query: 97 EEQVRKVRAGNQEKFQQLESAIQEK 171 EE+ R ++A NQ +QLE AIQE+ Sbjct: 374 EEERRALKADNQTLQKQLEKAIQER 398 >SPACUNK12.02c |cmk1||calcium/calmodulin-dependent protein kinase Cmk1|Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 27.5 bits (58), Expect = 1.8 Identities = 17/45 (37%), Positives = 23/45 (51%), Gaps = 1/45 (2%) Frame = +3 Query: 240 Y*TGGVRSAAT-AKVEEITKDIENKLTTAELNREKEIQKKLDFVK 371 Y G V T A V E NK+ A++ +K ++KK DFVK Sbjct: 31 YRVGRVLGGGTYATVREAVHIETNKMYAAKIMNKKMMEKKQDFVK 75 >SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 406 Score = 27.5 bits (58), Expect = 1.8 Identities = 22/72 (30%), Positives = 32/72 (44%), Gaps = 3/72 (4%) Frame = +1 Query: 100 EQVRKVRAGNQEKFQQLESAIQEKLQQAADRRLLIEAEQREKLRNHNIKLAESAR---PR 270 EQ+RK + + + E Q KLQ+ + R E EQR + ++A S R R Sbjct: 26 EQLRKAQIREKAREITEERNRQRKLQRERELRQKYEEEQRRQQAMEAKRIAASTRQTSER 85 Query: 271 PLKSRRSLKTLR 306 P S K +R Sbjct: 86 PPLSAEEAKRIR 97 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 27.1 bits (57), Expect = 2.4 Identities = 17/50 (34%), Positives = 26/50 (52%) Frame = -2 Query: 636 LLHYSHHVRFTLSVVLLIRNGVFHGFSVNVSNLTVDVGGRVTESNLTVVT 487 L +Y + VR S V I+ HG ++ S L + +G VT +L +VT Sbjct: 485 LSNYENPVRRVRSFVAEIQTFDIHGPILSGSTLVLHLGRTVTSVSLKIVT 534 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 26.2 bits (55), Expect = 4.1 Identities = 19/75 (25%), Positives = 37/75 (49%), Gaps = 4/75 (5%) Frame = +1 Query: 13 KAIEDKMTTAADKRDENLKKMIERL--REHEEQVRKV--RAGNQEKFQQLESAIQEKLQQ 180 KA + A + +EN K+ E RE EE+ ++ +E ++ + +EK ++ Sbjct: 584 KAKREAEEKAKREAEENAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKR 643 Query: 181 AADRRLLIEAEQREK 225 A+ + EAE++ K Sbjct: 644 EAEEKAKREAEEKAK 658 Score = 25.0 bits (52), Expect = 9.5 Identities = 19/70 (27%), Positives = 35/70 (50%), Gaps = 2/70 (2%) Frame = +1 Query: 22 EDKMTTAADKRDENLKKMIERLREHEEQVRKVRAGN--QEKFQQLESAIQEKLQQAADRR 195 +D + A +R + K E R+ EE+ R N +E +Q + +EK ++ A+ + Sbjct: 526 KDAIKLAIQQRIQE-KAEAEAKRKAEEKARLEAEENAKREAEEQAKREAEEKAKREAEEK 584 Query: 196 LLIEAEQREK 225 EAE++ K Sbjct: 585 AKREAEEKAK 594 >SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 1462 Score = 26.2 bits (55), Expect = 4.1 Identities = 16/34 (47%), Positives = 19/34 (55%) Frame = -3 Query: 323 GSGQFVLNVFSDLLDFSGRGRADSASLILWLRSF 222 GS +LN F DF G+ R D A L L L+SF Sbjct: 607 GSNSHILNAFISAFDFKGK-RIDEA-LRLLLQSF 638 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 25.8 bits (54), Expect = 5.5 Identities = 21/81 (25%), Positives = 40/81 (49%) Frame = +1 Query: 64 LKKMIERLREHEEQVRKVRAGNQEKFQQLESAIQEKLQQAADRRLLIEAEQREKLRNHNI 243 L + E+L + E+ K++ E +++++ E +QA+++ L E E+ EKL + Sbjct: 210 LLTVTEKLDKKEKDYEKIK----EDVSSIKASLAE--EQASNKSLRGEQERLEKLLVSSN 263 Query: 244 KLAESARPRPLKSRRSLKTLR 306 K + R R KTL+ Sbjct: 264 KTVSTLRQTENSLRAECKTLQ 284 >SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyces pombe|chr 3|||Manual Length = 1088 Score = 25.4 bits (53), Expect = 7.2 Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 2/39 (5%) Frame = +2 Query: 440 LLRLERGPPTHASHAHVTTVRFDSVTRPPTS--TVRFDT 550 + +LE P HAS +H +++ SV PTS +FDT Sbjct: 422 MTKLETKTPKHASSSHKSSIM--SVNSDPTSPKVSKFDT 458 >SPCC188.12 |spn6|SPCC584.09|septin Spn6|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 25.4 bits (53), Expect = 7.2 Identities = 11/38 (28%), Positives = 21/38 (55%) Frame = +1 Query: 10 YKAIEDKMTTAADKRDENLKKMIERLREHEEQVRKVRA 123 ++A E+KM D + L + ER+R E Q+ +++ Sbjct: 335 FQAKEEKMIETRDALNSELSEYHERIRALETQIESLKS 372 >SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy |Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 7.2 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = +3 Query: 294 KDIENKLTTAELNREKEIQKKLDFV 368 K I+N +TT E + ++ +Q +DFV Sbjct: 269 KRIKNLITTTEDSLQQSVQSNIDFV 293 >SPBC577.05c |rec27|mug41|meiotic recombination protein Rec27|Schizosaccharomyces pombe|chr 2|||Manual Length = 134 Score = 25.4 bits (53), Expect = 7.2 Identities = 13/48 (27%), Positives = 28/48 (58%), Gaps = 3/48 (6%) Frame = +1 Query: 130 QEKFQQLESAIQEKLQQAADRRLLIEAEQRE---KLRNHNIKLAESAR 264 Q+K ++ E +++Q + +L IEA +++ ++RN ++ AE R Sbjct: 72 QQKIKEFEIQKANQIKQLNEEKLSIEARKQQLEIEIRNQLLQYAEKLR 119 >SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizosaccharomyces pombe|chr 2|||Manual Length = 778 Score = 25.0 bits (52), Expect = 9.5 Identities = 18/53 (33%), Positives = 26/53 (49%), Gaps = 1/53 (1%) Frame = +3 Query: 252 GVRSAATAKVEEITKDIENKLTTAELNREKEIQKKLDF-VKXXERRAELVRQN 407 G ++ T + DI L + L +EK+I KKL F K E + + RQN Sbjct: 513 GAKNIQTGSIFWTFDDIIG-LKDSRLKKEKKIPKKLPFWKKYLELQLTMFRQN 564 >SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1194 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/29 (44%), Positives = 19/29 (65%) Frame = +1 Query: 19 IEDKMTTAADKRDENLKKMIERLREHEEQ 105 I D+ ++K DE L+ + ERLRE EE+ Sbjct: 178 IMDETIQKSEKIDELLQYIEERLRELEEE 206 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,865,918 Number of Sequences: 5004 Number of extensions: 27378 Number of successful extensions: 153 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 132 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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