Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20556
(693 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 217 1e-57
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 117 1e-27
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 114 1e-26
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 106 3e-24
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 30 0.36
SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 29 0.84
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.9
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.6
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.4
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 4.5
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.9
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 217 bits (530), Expect = 1e-57
Identities = 94/134 (70%), Positives = 112/134 (83%)
Frame = +3
Query: 60 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGQVR 239
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GG+
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 240 APRHPVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 419
VDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 420 RKESESCDCLQGFQ 461
R+E+E+CD LQGFQ
Sbjct: 121 RREAEACDALQGFQ 134
Score = 107 bits (256), Expect = 2e-24
Identities = 49/63 (77%), Positives = 56/63 (88%)
Frame = +2
Query: 500 GHPLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL 679
G L+SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL
Sbjct: 148 GTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEAL 207
Query: 680 YDI 688
I
Sbjct: 208 SSI 210
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 117 bits (282), Expect = 1e-27
Identities = 55/135 (40%), Positives = 80/135 (59%), Gaps = 2/135 (1%)
Frame = +3
Query: 60 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGQ 233
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E G+
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 234 VRAPRHPVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 413
VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 414 VVRKESESCDCLQGF 458
+R+ +++C LQGF
Sbjct: 121 KIRRIADNCSGLQGF 135
Score = 64.5 bits (150), Expect = 1e-11
Identities = 27/65 (41%), Positives = 43/65 (66%)
Frame = +2
Query: 497 YGHPLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEA 676
+G L+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+
Sbjct: 149 FGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNES 208
Query: 677 LYDIC 691
YDIC
Sbjct: 209 CYDIC 213
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 114 bits (274), Expect = 1e-26
Identities = 60/141 (42%), Positives = 86/141 (60%), Gaps = 8/141 (5%)
Frame = +3
Query: 60 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASG---- 227
MRE++ + GQ G QIG WE+ EHGI P G +S++ + N Y N+ G
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVH--KNNSYLNDGFGTFFS 58
Query: 228 --GQVR-APRHP-VDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAEL 395
GQ + PR VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E+
Sbjct: 59 ETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118
Query: 396 VDSVLDVVRKESESCDCLQGF 458
+DSVL+ +R+ +++C LQGF
Sbjct: 119 IDSVLERIRRMADNCSGLQGF 139
Score = 68.1 bits (159), Expect = 1e-12
Identities = 29/64 (45%), Positives = 44/64 (68%)
Frame = +2
Query: 500 GHPLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL 679
G L+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA
Sbjct: 154 GALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEAC 213
Query: 680 YDIC 691
YDIC
Sbjct: 214 YDIC 217
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 106 bits (255), Expect = 3e-24
Identities = 47/134 (35%), Positives = 84/134 (62%), Gaps = 2/134 (1%)
Frame = +3
Query: 63 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGQVRA 242
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 243 PRHPVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 416
+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 417 VRKESESCDCLQGF 458
+ +E++ D L+GF
Sbjct: 122 IDREADGSDSLEGF 135
Score = 58.0 bits (134), Expect = 1e-09
Identities = 28/64 (43%), Positives = 40/64 (62%), Gaps = 1/64 (1%)
Frame = +2
Query: 500 GHPLISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEA 676
G L+ ++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN A
Sbjct: 150 GSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAA 209
Query: 677 LYDI 688
L I
Sbjct: 210 LAHI 213
>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 29.9 bits (64), Expect = 0.36
Identities = 24/69 (34%), Positives = 38/69 (55%), Gaps = 11/69 (15%)
Frame = +2
Query: 494 RYGHPLISKIREEYP-DRIMNTYSVVP--SPKVSDTVVE---PYN--ATLSVHQL---VE 640
+Y H + SK++ EYP D+++ SV+ PK VVE P N A L+ H + ++
Sbjct: 392 QYAHVVASKLQREYPRDKVLYYSSVLTEFDPKGIQEVVESLRPNNFFAILAAHSIEKGLD 451
Query: 641 NTDETYCID 667
N ++ Y ID
Sbjct: 452 NKEKFYGID 460
>SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 682
Score = 28.7 bits (61), Expect = 0.84
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +1
Query: 460 NLHIPSVAAPGPVWAPSHLKDP*RVPRQNHEHILSSPLAQS 582
N+H PS A + +++P +P+QNHEH SPL +S
Sbjct: 623 NIHHPSANLLDASAALNPVQNPLLMPQQNHEH---SPLVRS 660
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 1.9
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +3
Query: 363 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 452
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +3
Query: 99 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 200
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 294 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 395
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 4.5
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +1
Query: 43 KITYTK*GKSFIYRPANVV 99
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 7.9
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +3
Query: 315 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 446
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -3
Query: 352 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 257
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,788,308
Number of Sequences: 5004
Number of extensions: 57549
Number of successful extensions: 176
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -