BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20549 (717 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC338.15 |||dolichyl-di-phosphooligosaccharide-protein glycotr... 35 0.013 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 3.5 >SPCC338.15 |||dolichyl-di-phosphooligosaccharide-protein glycotransferase subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 437 Score = 34.7 bits (76), Expect = 0.013 Identities = 17/48 (35%), Positives = 26/48 (54%) Frame = +2 Query: 125 HSQFFKSLQERGYGLTFKLADDANLVLSKYGEYLYKNLIVFAPSCSSL 268 +S F +SL +R + + D + L +YGE LY NLI+ + SL Sbjct: 35 YSTFLQSLTDRDFDVKTSYIKDESAKLFEYGERLYDNLILLSSQSKSL 82 Score = 27.9 bits (59), Expect = 1.5 Identities = 13/38 (34%), Positives = 22/38 (57%) Frame = +3 Query: 525 PLLFEGTGLIVDKDNSLVLPILSADSTAYSYNPKSQVK 638 P+L++G G + N PIL + T+Y YN K++ + Sbjct: 166 PILYKGIGHYLGP-NPQTQPILRGNPTSYIYNTKTEAE 202 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 26.6 bits (56), Expect = 3.5 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = -2 Query: 338 LPAIRRFPPSSMNLVMASLSTCPPNSST 255 +P++ + PPSS+ A ST PP+ S+ Sbjct: 1498 IPSVAQQPPSSVAPATAPSSTLPPSQSS 1525 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,879,573 Number of Sequences: 5004 Number of extensions: 58399 Number of successful extensions: 195 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 182 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 195 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 335201398 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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