Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20549
(717 letters)
Database: fruitfly
53,049 sequences; 24,988,368 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
X81999-1|CAA57525.1| 449|Drosophila melanogaster oligosaccharyl... 100 2e-21
BT010050-1|AAQ22519.1| 449|Drosophila melanogaster LD21941p pro... 100 2e-21
AE014298-1289|AAF46453.1| 449|Drosophila melanogaster CG9022-PA... 100 2e-21
X81207-1|CAA57079.1| 330|Drosophila melanogaster oligosaccharyl... 75 1e-13
>X81999-1|CAA57525.1| 449|Drosophila melanogaster
oligosaccharyltransferase subunit protein.
Length = 449
Score = 100 bits (239), Expect = 2e-21
Identities = 45/60 (75%), Positives = 55/60 (91%)
Frame = +2
Query: 77 DHETLVLIDNLNIKETHSQFFKSLQERGYGLTFKLADDANLVLSKYGEYLYKNLIVFAPS 256
D TLVL+DNL I+ETHS FFKSLQ+RG+ LT+KLADD++L+LSKYGEYLYKN+I+FAPS
Sbjct: 23 DANTLVLLDNLAIRETHSIFFKSLQDRGFKLTYKLADDSSLLLSKYGEYLYKNVIIFAPS 82
Score = 99.5 bits (237), Expect = 4e-21
Identities = 47/84 (55%), Positives = 57/84 (67%)
Frame = +1
Query: 256 VLEFGGQVDSEAITKFIDDXXXXXXXXXXXXXDVYREIASECGFEMDEESAAVIDHFNYD 435
V EFGG V EA+ +F+DD D RE ASECGFE+DEE+AAVIDH +YD
Sbjct: 83 VEEFGGDVSVEALAQFVDDGGNVLVAGSEKSGDALREFASECGFELDEENAAVIDHLHYD 142
Query: 436 VTDEGDHTRIVVSPKNLIKAPTIV 507
V+D G+HT I+ S KNLI+A TIV
Sbjct: 143 VSDAGEHTTILTSAKNLIQADTIV 166
Score = 67.3 bits (157), Expect = 2e-11
Identities = 28/47 (59%), Positives = 38/47 (80%)
Frame = +3
Query: 507 REQNTQPLLFEGTGLIVDKDNSLVLPILSADSTAYSYNPKSQVKEYP 647
R+ + PLL+ GTGLI DK+N LVL +L+A+STAYSYNP++ V +YP
Sbjct: 171 RQADAAPLLYRGTGLIADKENPLVLKLLTAESTAYSYNPEASVSDYP 217
Score = 46.8 bits (106), Expect = 3e-05
Identities = 21/24 (87%), Positives = 23/24 (95%)
Frame = +2
Query: 644 PHAVGRKTVLIAALQARNNARIVF 715
PHAVGR T+LIAALQARNNAR+VF
Sbjct: 217 PHAVGRGTLLIAALQARNNARVVF 240
>BT010050-1|AAQ22519.1| 449|Drosophila melanogaster LD21941p
protein.
Length = 449
Score = 100 bits (239), Expect = 2e-21
Identities = 45/60 (75%), Positives = 55/60 (91%)
Frame = +2
Query: 77 DHETLVLIDNLNIKETHSQFFKSLQERGYGLTFKLADDANLVLSKYGEYLYKNLIVFAPS 256
D TLVL+DNL I+ETHS FFKSLQ+RG+ LT+KLADD++L+LSKYGEYLYKN+I+FAPS
Sbjct: 23 DANTLVLLDNLAIRETHSIFFKSLQDRGFKLTYKLADDSSLLLSKYGEYLYKNVIIFAPS 82
Score = 97.5 bits (232), Expect = 2e-20
Identities = 46/84 (54%), Positives = 56/84 (66%)
Frame = +1
Query: 256 VLEFGGQVDSEAITKFIDDXXXXXXXXXXXXXDVYREIASECGFEMDEESAAVIDHFNYD 435
V EFGG V E + +F+DD D RE ASECGFE+DEE+AAVIDH +YD
Sbjct: 83 VEEFGGDVSVERLAQFVDDGGNVLVAGSEKSGDALREFASECGFELDEENAAVIDHLHYD 142
Query: 436 VTDEGDHTRIVVSPKNLIKAPTIV 507
V+D G+HT I+ S KNLI+A TIV
Sbjct: 143 VSDAGEHTTILTSAKNLIQADTIV 166
Score = 67.3 bits (157), Expect = 2e-11
Identities = 28/47 (59%), Positives = 38/47 (80%)
Frame = +3
Query: 507 REQNTQPLLFEGTGLIVDKDNSLVLPILSADSTAYSYNPKSQVKEYP 647
R+ + PLL+ GTGLI DK+N LVL +L+A+STAYSYNP++ V +YP
Sbjct: 171 RQADAAPLLYRGTGLIADKENPLVLKLLTAESTAYSYNPEASVSDYP 217
Score = 46.8 bits (106), Expect = 3e-05
Identities = 21/24 (87%), Positives = 23/24 (95%)
Frame = +2
Query: 644 PHAVGRKTVLIAALQARNNARIVF 715
PHAVGR T+LIAALQARNNAR+VF
Sbjct: 217 PHAVGRGTLLIAALQARNNARVVF 240
>AE014298-1289|AAF46453.1| 449|Drosophila melanogaster CG9022-PA
protein.
Length = 449
Score = 100 bits (239), Expect = 2e-21
Identities = 45/60 (75%), Positives = 55/60 (91%)
Frame = +2
Query: 77 DHETLVLIDNLNIKETHSQFFKSLQERGYGLTFKLADDANLVLSKYGEYLYKNLIVFAPS 256
D TLVL+DNL I+ETHS FFKSLQ+RG+ LT+KLADD++L+LSKYGEYLYKN+I+FAPS
Sbjct: 23 DANTLVLLDNLAIRETHSIFFKSLQDRGFKLTYKLADDSSLLLSKYGEYLYKNVIIFAPS 82
Score = 97.5 bits (232), Expect = 2e-20
Identities = 46/84 (54%), Positives = 56/84 (66%)
Frame = +1
Query: 256 VLEFGGQVDSEAITKFIDDXXXXXXXXXXXXXDVYREIASECGFEMDEESAAVIDHFNYD 435
V EFGG V E + +F+DD D RE ASECGFE+DEE+AAVIDH +YD
Sbjct: 83 VEEFGGDVSVERLAQFVDDGGNVLVAGSEKSGDALREFASECGFELDEENAAVIDHLHYD 142
Query: 436 VTDEGDHTRIVVSPKNLIKAPTIV 507
V+D G+HT I+ S KNLI+A TIV
Sbjct: 143 VSDAGEHTTILTSAKNLIQADTIV 166
Score = 67.3 bits (157), Expect = 2e-11
Identities = 28/47 (59%), Positives = 38/47 (80%)
Frame = +3
Query: 507 REQNTQPLLFEGTGLIVDKDNSLVLPILSADSTAYSYNPKSQVKEYP 647
R+ + PLL+ GTGLI DK+N LVL +L+A+STAYSYNP++ V +YP
Sbjct: 171 RQADAAPLLYRGTGLIADKENPLVLKLLTAESTAYSYNPEASVSDYP 217
Score = 46.8 bits (106), Expect = 3e-05
Identities = 21/24 (87%), Positives = 23/24 (95%)
Frame = +2
Query: 644 PHAVGRKTVLIAALQARNNARIVF 715
PHAVGR T+LIAALQARNNAR+VF
Sbjct: 217 PHAVGRGTLLIAALQARNNARVVF 240
>X81207-1|CAA57079.1| 330|Drosophila melanogaster
oligosaccharyltransferase 48kDasubunit protein.
Length = 330
Score = 74.9 bits (176), Expect = 1e-13
Identities = 34/48 (70%), Positives = 40/48 (83%)
Frame = +1
Query: 364 EIASECGFEMDEESAAVIDHFNYDVTDEGDHTRIVVSPKNLIKAPTIV 507
E ASECGFE+DEE+AAVIDH +YDV+D GD T I+ S KNLI+A TIV
Sbjct: 1 EFASECGFELDEENAAVIDHLHYDVSDAGDDTTILTSAKNLIQADTIV 48
Score = 66.1 bits (154), Expect = 4e-11
Identities = 28/45 (62%), Positives = 37/45 (82%)
Frame = +3
Query: 513 QNTQPLLFEGTGLIVDKDNSLVLPILSADSTAYSYNPKSQVKEYP 647
Q +PLL+ GTGLI DK+N LVL +L+A+STAYSYNP++ V +YP
Sbjct: 54 QADRPLLYRGTGLIADKENPLVLKLLTAESTAYSYNPEASVSDYP 98
Score = 46.8 bits (106), Expect = 3e-05
Identities = 21/24 (87%), Positives = 23/24 (95%)
Frame = +2
Query: 644 PHAVGRKTVLIAALQARNNARIVF 715
PHAVGR TV+IAALQARNNAR+VF
Sbjct: 98 PHAVGRGTVVIAALQARNNARVVF 121
Database: fruitfly
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 24,988,368
Number of sequences in database: 53,049
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 31,108,146
Number of Sequences: 53049
Number of extensions: 645841
Number of successful extensions: 1661
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 1589
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1661
length of database: 24,988,368
effective HSP length: 83
effective length of database: 20,585,301
effective search space used: 3190721655
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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