Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20546
(649 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B) 117 6e-27
At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) riboso... 113 7e-26
At3g24080.1 68416.m03024 KRR1 family protein contains Pfam PF051... 30 1.5
At1g21740.1 68414.m02721 expressed protein contains Pfam domains... 30 1.5
At1g67280.1 68414.m07657 lactoylglutathione lyase, putative / gl... 29 3.5
At1g21390.1 68414.m02676 expressed protein 28 4.7
At2g16390.1 68415.m01876 SNF2 domain-containing protein / helica... 28 6.1
At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP... 27 8.1
>At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B)
Length = 249
Score = 117 bits (282), Expect = 6e-27
Identities = 63/105 (60%), Positives = 75/105 (71%), Gaps = 5/105 (4%)
Frame = +2
Query: 257 KGHSCYRP--RRDGERKRKSVRGCIVDANLSVLALVIVRKGAQEIPGLTDGNVPRRLGPK 430
+G C+R RR GER+RKSVRGCIV +LSVL LVIV+KG ++PGLTD PR GPK
Sbjct: 79 RGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKKGVSDLPGLTDTEKPRMRGPK 138
Query: 431 RASKIRKLFNLSKEDDVRRYV--VKRVLQPRKEKKMLN-PDIRHL 556
RASKIRKLFNL KEDDVR+YV +R +K KK+ P I+ L
Sbjct: 139 RASKIRKLFNLGKEDDVRKYVNTYRRTFTNKKGKKVSKAPKIQRL 183
Score = 112 bits (270), Expect = 2e-25
Identities = 50/81 (61%), Positives = 61/81 (75%)
Frame = +3
Query: 24 MKLNVSYPATGCQKLFEVVDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVAGGNDKQG 203
MK NV+ P TGCQK E+ D+ KLR F++KR+ EV D LG+E+KGYV ++ GG DKQG
Sbjct: 1 MKFNVANPTTGCQKKLEIDDDQKLRAFFDKRLSQEVSGDALGEEFKGYVFKIMGGCDKQG 60
Query: 204 FPMKQGVLTNSRVRLLMQRAT 266
FPMKQGVLT RVRLL+ R T
Sbjct: 61 FPMKQGVLTPGRVRLLLHRGT 81
Score = 30.7 bits (66), Expect = 0.87
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = +1
Query: 526 ENAKPRHKAPKIQRLVTPVVLQ 591
+ K KAPKIQRLVTP+ LQ
Sbjct: 169 KKGKKVSKAPKIQRLVTPLTLQ 190
>At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) ribosomal
protein S6, Arabidopsis thaliana, PID:g2662469
Length = 250
Score = 113 bits (273), Expect = 7e-26
Identities = 51/81 (62%), Positives = 61/81 (75%)
Frame = +3
Query: 24 MKLNVSYPATGCQKLFEVVDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVAGGNDKQG 203
MK NV+ P TGCQK E+ D+ KLR FY+KR+ EV D LG+E+KGYV ++ GG DKQG
Sbjct: 1 MKFNVANPTTGCQKKLEIDDDQKLRAFYDKRISQEVSGDALGEEFKGYVFKIKGGCDKQG 60
Query: 204 FPMKQGVLTNSRVRLLMQRAT 266
FPMKQGVLT RVRLL+ R T
Sbjct: 61 FPMKQGVLTPGRVRLLLHRGT 81
Score = 113 bits (272), Expect = 1e-25
Identities = 62/105 (59%), Positives = 74/105 (70%), Gaps = 5/105 (4%)
Frame = +2
Query: 257 KGHSCYRP--RRDGERKRKSVRGCIVDANLSVLALVIVRKGAQEIPGLTDGNVPRRLGPK 430
+G C+R RR GER+RKSVRGCIV +LSVL LVIV+KG ++PGLTD PR GPK
Sbjct: 79 RGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKKGENDLPGLTDTEKPRMRGPK 138
Query: 431 RASKIRKLFNLSKEDDVRRYV--VKRVLQPRKEKKMLN-PDIRHL 556
RASKIRKLFNL KEDDVR YV +R +K K++ P I+ L
Sbjct: 139 RASKIRKLFNLKKEDDVRTYVNTYRRKFTNKKGKEVSKAPKIQRL 183
Score = 31.5 bits (68), Expect = 0.50
Identities = 17/26 (65%), Positives = 20/26 (76%)
Frame = +1
Query: 514 KEGKENAKPRHKAPKIQRLVTPVVLQ 591
K+GKE +K APKIQRLVTP+ LQ
Sbjct: 169 KKGKEVSK----APKIQRLVTPLTLQ 190
>At3g24080.1 68416.m03024 KRR1 family protein contains Pfam PF05178:
Krr1 family
Length = 638
Score = 29.9 bits (64), Expect = 1.5
Identities = 13/45 (28%), Positives = 28/45 (62%)
Frame = +2
Query: 392 LTDGNVPRRLGPKRASKIRKLFNLSKEDDVRRYVVKRVLQPRKEK 526
+ +G+V ++ ++A + K + KEDDVR+ +KR+ +K++
Sbjct: 288 VVEGSVRKKDNARKAQRKNKDERMKKEDDVRKEELKRLKNVKKKE 332
>At1g21740.1 68414.m02721 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 953
Score = 29.9 bits (64), Expect = 1.5
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = +3
Query: 9 GLTAVMKLNVSYPATGCQKLF-EVVDEHKLRIFYEKRMGAEVEADQLGDE 155
GLT + + +KL+ EV DE KLR+ YE++ + D LG E
Sbjct: 585 GLTGNLSATLEQLYAWEKKLYKEVKDEEKLRVVYEEKCRTLKKLDSLGAE 634
>At1g67280.1 68414.m07657 lactoylglutathione lyase, putative /
glyoxalase I, putative similar to putative
lactoylglutathione lyase SP:Q39366, GI:2494843 from
[Brassica oleracea]
Length = 350
Score = 28.7 bits (61), Expect = 3.5
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +3
Query: 45 PATGCQKLFEVVDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVAGGNDKQGFPM 212
P CQ + V D + FYEK G E+ + E+K Y + + G + FP+
Sbjct: 216 PEPLCQVMLRVGDLDRAIKFYEKAFGMELLRTRDNPEYK-YTIAMMGYGPEDKFPV 270
>At1g21390.1 68414.m02676 expressed protein
Length = 248
Score = 28.3 bits (60), Expect = 4.7
Identities = 29/110 (26%), Positives = 45/110 (40%), Gaps = 1/110 (0%)
Frame = +2
Query: 161 GLCTSCRWRQRQARIPDETGRPDKQPCSSSDAKGHSCYRPRRD-GERKRKSVRGCIVDAN 337
G+C+ WR + P RP+ S D+K + R +R+ E K C
Sbjct: 28 GVCSPTLWRTSPPKSPPPFHRPEDYWSLSPDSKAQAIARGQRELMEMVSKMPESCY---E 84
Query: 338 LSVLALVIVRKGAQEIPGLTDGNVPRRLGPKRASKIRKLFNLSKEDDVRR 487
LS+ LV V+ + + D +P+R R SK+ + K D R
Sbjct: 85 LSLKDLVEVKVNQENERKVFD-ELPKR--ANRQSKVVRKTKSDKRVDPNR 131
>At2g16390.1 68415.m01876 SNF2 domain-containing protein / helicase
domain-containing protein low similarity to RAD54
[Drosophila melanogaster] GI:1765914; contains Pfam
profiles PF00271: Helicase conserved C-terminal domain,
PF00176: SNF2 family N-terminal domain
Length = 888
Score = 27.9 bits (59), Expect = 6.1
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +2
Query: 380 EIPGLTDGNVPRRLGPKRASKIRKL 454
E+PGL D V L PK+ ++++KL
Sbjct: 620 ELPGLADFTVVLNLSPKQLNEVKKL 644
>At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP2)
identical to fatty acid multifunctional protein (AtMFP2)
GB:AF123254 [gi:4337027] (Arabidopsis thaliana) (fatty
acid beta-oxidation); contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 725
Score = 27.5 bits (58), Expect = 8.1
Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 3/67 (4%)
Frame = +2
Query: 230 KQPCSSSDAKGHSCYRPRRDGERKRKSVRGCIV--DANLSVLALVIVRKGAQEIPGLTD- 400
K P DA R G K V +V D ++ + ++G ++PG+TD
Sbjct: 246 KHPLMCLDAIEVGIVSGPRAGLEKEAEVASQVVKLDTTKGLIHVFFSQRGTAKVPGVTDR 305
Query: 401 GNVPRRL 421
G VPR++
Sbjct: 306 GLVPRKI 312
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,080,015
Number of Sequences: 28952
Number of extensions: 303611
Number of successful extensions: 881
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 857
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 881
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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