BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20543 (453 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 139 2e-34 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 98 6e-22 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 91 1e-19 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 83 2e-17 SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 30 0.14 SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom... 28 0.58 SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr ... 28 0.77 SPBC2D10.06 |rep1|rec16|MBF transcription factor complex subunit... 27 1.8 SPBC36.11 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 27 1.8 SPAC26H5.11 |||spore wall assembly protein |Schizosaccharomyces ... 26 3.1 SPAPB1E7.08c |||membrane transporter|Schizosaccharomyces pombe|c... 25 4.1 SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 5.4 SPAC6B12.13 |||protein phosphatase inhibitor |Schizosaccharomyce... 25 7.2 SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|... 24 9.5 SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protei... 24 9.5 SPBC902.06 |mto2||MT organizer Mto2|Schizosaccharomyces pombe|ch... 24 9.5 SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe... 24 9.5 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 139 bits (336), Expect = 2e-34 Identities = 63/84 (75%), Positives = 73/84 (86%) Frame = +3 Query: 3 GGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNAVLSIHQLVENTDETY 182 GGTGSGMGTLL+SKIREEYPDR+M T+SV P+PK SDTVVEPYNA LS+HQLVEN+DET+ Sbjct: 141 GGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETF 200 Query: 183 CIDNEALYDICYRTLKVPNPTYGD 254 CIDNEAL I TLK+ +P+Y D Sbjct: 201 CIDNEALSSIFANTLKIKSPSYDD 224 Score = 101 bits (243), Expect = 4e-23 Identities = 46/71 (64%), Positives = 56/71 (78%) Frame = +2 Query: 239 PNVRRLNHLVSLTMSGVTTCLRFPGQLNADLRNWSVNMVPFPRLHFFMPGFAPLTSRGSQ 418 P+ LNHLVS M+GVTT RFPG+LN+DLR +VNMVPFPRLHFFM GFAPL + GS Sbjct: 220 PSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSS 279 Query: 419 QYRALTVPALT 451 ++A++VP LT Sbjct: 280 SFQAVSVPELT 290 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 97.9 bits (233), Expect = 6e-22 Identities = 42/82 (51%), Positives = 59/82 (71%) Frame = +3 Query: 3 GGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNAVLSIHQLVENTDETY 182 GGTGSG+G LL+ ++ EY + +SV P+P+VS +VVEPYN+VL+ H ++N+D T+ Sbjct: 147 GGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTF 206 Query: 183 CIDNEALYDICYRTLKVPNPTY 248 +DNEA YDIC R L + PTY Sbjct: 207 MVDNEACYDICRRNLDIERPTY 228 Score = 60.9 bits (141), Expect = 9e-11 Identities = 25/71 (35%), Positives = 41/71 (57%) Frame = +2 Query: 239 PNVRRLNHLVSLTMSGVTTCLRFPGQLNADLRNWSVNMVPFPRLHFFMPGFAPLTSRGSQ 418 P LN L++ +S +T LRF G LN DL + N+VP+PR+HF + ++P+ S Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKA 285 Query: 419 QYRALTVPALT 451 + + +V +T Sbjct: 286 FHESNSVQEIT 296 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 90.6 bits (215), Expect = 1e-19 Identities = 39/82 (47%), Positives = 56/82 (68%) Frame = +3 Query: 3 GGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNAVLSIHQLVENTDETY 182 GGTGSG G LL+ ++ EY + +SV P+P+VS +VVEPYN+VL+ H ++ D T+ Sbjct: 143 GGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTF 202 Query: 183 CIDNEALYDICYRTLKVPNPTY 248 +DNE+ YDIC R L + P+Y Sbjct: 203 MVDNESCYDICRRNLDIERPSY 224 Score = 62.1 bits (144), Expect = 4e-11 Identities = 26/71 (36%), Positives = 42/71 (59%) Frame = +2 Query: 239 PNVRRLNHLVSLTMSGVTTCLRFPGQLNADLRNWSVNMVPFPRLHFFMPGFAPLTSRGSQ 418 P+ LN L++ +S +T LRF G LN DL + N+VP+PR+HF + +AP+ S Sbjct: 222 PSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKA 281 Query: 419 QYRALTVPALT 451 + + +V +T Sbjct: 282 FHESNSVQEIT 292 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 83.0 bits (196), Expect = 2e-17 Identities = 38/83 (45%), Positives = 54/83 (65%), Gaps = 1/83 (1%) Frame = +3 Query: 3 GGTGSGMGTLLISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNAVLSIHQLVENTDET 179 GGTGSG+G+ L+ ++ + YP +I+ TYSV P S VSD VV+PYN++L++ +L N D Sbjct: 143 GGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSV 202 Query: 180 YCIDNEALYDICYRTLKVPNPTY 248 +DN AL I L NPT+ Sbjct: 203 VVLDNAALAHIAADRLHTQNPTF 225 Score = 57.6 bits (133), Expect = 8e-10 Identities = 27/78 (34%), Positives = 45/78 (57%), Gaps = 1/78 (1%) Frame = +2 Query: 203 LRYLLQDSQGTE-PNVRRLNHLVSLTMSGVTTCLRFPGQLNADLRNWSVNMVPFPRLHFF 379 L ++ D T+ P + N LVS MS TT LR+PG +N DL + +++P PR HF Sbjct: 210 LAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFL 269 Query: 380 MPGFAPLTSRGSQQYRAL 433 + + P T++ ++ +A+ Sbjct: 270 LTSYTPFTNQQVEEAKAI 287 >SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1327 Score = 30.3 bits (65), Expect = 0.14 Identities = 22/79 (27%), Positives = 41/79 (51%), Gaps = 1/79 (1%) Frame = +2 Query: 41 KDPRRVPRQNHEHILSSPLAQSIRHCRRTIQRSSLHPSTSREYRRNLLHRQRGPLRY-LL 217 K P + ++N + L+S S+ H + + +L S E RR+L + R + +L Sbjct: 210 KPPPKASQKNEKVSLTSIKNTSLSHFSKFNFKYALPIHMSLENRRSLENLFRNMNTWDIL 269 Query: 218 QDSQGTEPNVRRLNHLVSL 274 +D++ EP+ +N L+SL Sbjct: 270 EDTKNLEPDTSIVNDLISL 288 >SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1021 Score = 28.3 bits (60), Expect = 0.58 Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 6/57 (10%) Frame = +3 Query: 84 SVVPSPKVSDTVVE------PYNAVLSIHQLVENTDETYCIDNEALYDICYRTLKVP 236 S+ P+P+ DTV+ PYNA+ +Q V+ T + A I Y K+P Sbjct: 924 SLTPNPQQQDTVINAVPTFAPYNAMTKFNQKVKVMPGTGKVGKAARESIAYFMKKLP 980 >SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 391 Score = 27.9 bits (59), Expect = 0.77 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +2 Query: 239 PNVRRLNHLVSLTMSGVTTCLRFPGQLN 322 P+ RL HLV + G++ C +PG+ N Sbjct: 326 PHKIRLTHLVDAPLLGMSVCPLYPGEEN 353 >SPBC2D10.06 |rep1|rec16|MBF transcription factor complex subunit Rep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 472 Score = 26.6 bits (56), Expect = 1.8 Identities = 14/59 (23%), Positives = 25/59 (42%) Frame = -1 Query: 450 VSAGTVSARYCWLPRDVSGAKPGMKKCRRGKGTMLTDQLRRSAFSCPGNRRHVVTPDMV 274 ++ G + R C + A+ K+ +GK T + + P RRH+ D+V Sbjct: 350 IAYGGIPMRVCHSDSTLCDARIAAKQALKGKRQYDTSTSKAELSTPPSKRRHIDDADLV 408 >SPBC36.11 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 343 Score = 26.6 bits (56), Expect = 1.8 Identities = 11/47 (23%), Positives = 22/47 (46%) Frame = -2 Query: 305 TAGTSSRRTWSATPGGSVAVRWVRYLESPVADIVKGLVVYAVSFVCI 165 +A T + S+ P G+ + P+ ++ GL+V + F C+ Sbjct: 73 SASTPPSASASSLPNGAQKHNHTGVIAGPIVGVLGGLIVLVIIFYCL 119 >SPAC26H5.11 |||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 965 Score = 25.8 bits (54), Expect = 3.1 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = +2 Query: 131 QRSSLHPSTSREYRRNLLHRQRG 199 Q SSLHPS+SR + + H RG Sbjct: 201 QLSSLHPSSSRRHLISTPHVNRG 223 >SPAPB1E7.08c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 554 Score = 25.4 bits (53), Expect = 4.1 Identities = 15/43 (34%), Positives = 21/43 (48%) Frame = +3 Query: 84 SVVPSPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNEALYDI 212 SV SPK + Y AV +IH++ +T + E LY I Sbjct: 272 SVYESPKFYLAKGDDYKAVETIHKIARINGKTCTLTVEELYAI 314 >SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 682 Score = 25.0 bits (52), Expect = 5.4 Identities = 11/23 (47%), Positives = 17/23 (73%) Frame = +2 Query: 38 LKDPRRVPRQNHEHILSSPLAQS 106 +++P +P+QNHEH SPL +S Sbjct: 641 VQNPLLMPQQNHEH---SPLVRS 660 >SPAC6B12.13 |||protein phosphatase inhibitor |Schizosaccharomyces pombe|chr 1|||Manual Length = 104 Score = 24.6 bits (51), Expect = 7.2 Identities = 14/42 (33%), Positives = 19/42 (45%) Frame = +2 Query: 83 LSSPLAQSIRHCRRTIQRSSLHPSTSREYRRNLLHRQRGPLR 208 L+ PL TI+ + S S E N+LH Q P+R Sbjct: 3 LTRPLGDISSSATVTIESTEESASISHEESENVLHLQPEPVR 44 >SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 24.2 bits (50), Expect = 9.5 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = -1 Query: 405 DVSGAKPGMKKCRRGKGTMLTDQLRRSAFSC 313 D + K G+K KGT+ + RSAF+C Sbjct: 181 DSATVKKGLKSGTLFKGTLRILENHRSAFAC 211 >SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1462 Score = 24.2 bits (50), Expect = 9.5 Identities = 8/20 (40%), Positives = 14/20 (70%) Frame = +3 Query: 129 YNAVLSIHQLVENTDETYCI 188 +N L+++ +V N DET+ I Sbjct: 1419 FNVCLAVNTIVNNVDETFSI 1438 >SPBC902.06 |mto2||MT organizer Mto2|Schizosaccharomyces pombe|chr 2|||Manual Length = 397 Score = 24.2 bits (50), Expect = 9.5 Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 1/45 (2%) Frame = +2 Query: 104 SIRHCRRTIQRSSLHPS-TSREYRRNLLHRQRGPLRYLLQDSQGT 235 S RH + +SSLHPS TS ++ ++ P Q T Sbjct: 350 STRHVSNSTNKSSLHPSPTSLRVAHPIIPQRASPASQSFPSLQDT 394 >SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe|chr 2|||Manual Length = 675 Score = 24.2 bits (50), Expect = 9.5 Identities = 14/49 (28%), Positives = 24/49 (48%), Gaps = 3/49 (6%) Frame = +3 Query: 63 DRIMNTYSVVPSPKVSDTVVEPYNAVLSIHQLVENTDETY---CIDNEA 200 D+ S+V T++EP + + H+L E +TY +D+EA Sbjct: 62 DKFEEVESLVYEFSTPSTLIEPNYLLTAWHELWEELQDTYMTPILDSEA 110 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,720,915 Number of Sequences: 5004 Number of extensions: 35847 Number of successful extensions: 134 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 128 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 133 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 168258430 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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