BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20539 (684 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 27 1.9 SPAC5H10.11 |gmh1||alpha-1,2-galactosyltransferase Gmh1|Schizosa... 27 3.3 SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 7.7 >SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 3699 Score = 27.5 bits (58), Expect = 1.9 Identities = 20/59 (33%), Positives = 28/59 (47%), Gaps = 1/59 (1%) Frame = -3 Query: 511 EATSISFGTAVGAGFNCTSTIATIAKPES-RADVLLEAFSFALLSCNFLHERYAGV*SP 338 E TS++ T + F C +A I +S R VLL F A ++ N E+ A V P Sbjct: 689 EVTSVNC-TGIVLSF-CCDNLAKIGSGQSTRVSVLLRLFKLAFMTVNVFPEKNAEVLRP 745 >SPAC5H10.11 |gmh1||alpha-1,2-galactosyltransferase Gmh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 26.6 bits (56), Expect = 3.3 Identities = 12/42 (28%), Positives = 20/42 (47%) Frame = +3 Query: 204 LVSQYWWKQLPLRFTRRRYPSSNWCPKLSKHKCVMSGETPIQ 329 +V W K + T R+YPS+ W L + +M+ +Q Sbjct: 132 VVPPVWGKMPAILQTMRKYPSAKWIWWLDQDALIMNKNLSLQ 173 >SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 669 Score = 25.4 bits (53), Expect = 7.7 Identities = 12/30 (40%), Positives = 15/30 (50%) Frame = +2 Query: 410 QNICPTFRFRYGGYGRSTIKSSPHCSTKGD 499 QN PTF YG Y + +P+ ST D Sbjct: 144 QNAVPTFHPEYGRYMLESTPGAPYGSTLKD 173 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,960,350 Number of Sequences: 5004 Number of extensions: 60892 Number of successful extensions: 147 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 140 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 147 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 315915086 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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