Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20536
(577 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 156 2e-39
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 156 2e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 156 2e-39
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 64 1e-11
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 54 2e-08
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 44 2e-05
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 27 1.5
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 2.0
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 27 2.6
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 3.4
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 6.0
SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 25 6.0
SPCC965.13 |||membrane transporter|Schizosaccharomyces pombe|chr... 25 6.0
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 25 7.9
SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 25 7.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 156 bits (379), Expect = 2e-39
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +3
Query: 255 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 434
EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEV
Sbjct: 228 EAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEV 287
Query: 435 KSVEMHHEALQEAVPGDNVGFNVK 506
KSVEMHHE+L +PGDNVGFNVK
Sbjct: 288 KSVEMHHESLDAGLPGDNVGFNVK 311
Score = 119 bits (287), Expect = 3e-28
Identities = 59/112 (52%), Positives = 77/112 (68%), Gaps = 1/112 (0%)
Frame = +1
Query: 1 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEP 180
VKQLIV VNKMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP
Sbjct: 145 VKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEP 202
Query: 181 STKMPWFKGWQVERKEGKADGKCLMKLSMPSCHLPA-PLTSPCVFPCKTYTK 333
+T MPW++GWQ E K G GK L++ ++ S PA P P P + K
Sbjct: 203 TTNMPWYQGWQKETKAGVVKGKTLLE-AIDSIEPPARPTDKPLRLPLQDVYK 253
Score = 41.9 bits (94), Expect = 6e-05
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = +2
Query: 494 FQRKEVSVKELRRGYVAGDSKNNPPKG 574
F K VSVK++RRG V GDSKN+PP G
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMG 334
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 156 bits (379), Expect = 2e-39
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +3
Query: 255 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 434
EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEV
Sbjct: 228 EAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEV 287
Query: 435 KSVEMHHEALQEAVPGDNVGFNVK 506
KSVEMHHE+L +PGDNVGFNVK
Sbjct: 288 KSVEMHHESLDAGLPGDNVGFNVK 311
Score = 119 bits (287), Expect = 3e-28
Identities = 59/112 (52%), Positives = 77/112 (68%), Gaps = 1/112 (0%)
Frame = +1
Query: 1 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEP 180
VKQLIV VNKMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP
Sbjct: 145 VKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEP 202
Query: 181 STKMPWFKGWQVERKEGKADGKCLMKLSMPSCHLPA-PLTSPCVFPCKTYTK 333
+T MPW++GWQ E K G GK L++ ++ S PA P P P + K
Sbjct: 203 TTNMPWYQGWQKETKAGVVKGKTLLE-AIDSIEPPARPTDKPLRLPLQDVYK 253
Score = 41.9 bits (94), Expect = 6e-05
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = +2
Query: 494 FQRKEVSVKELRRGYVAGDSKNNPPKG 574
F K VSVK++RRG V GDSKN+PP G
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMG 334
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 156 bits (379), Expect = 2e-39
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +3
Query: 255 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 434
EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEV
Sbjct: 228 EAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEV 287
Query: 435 KSVEMHHEALQEAVPGDNVGFNVK 506
KSVEMHHE+L +PGDNVGFNVK
Sbjct: 288 KSVEMHHESLDAGLPGDNVGFNVK 311
Score = 119 bits (287), Expect = 3e-28
Identities = 59/112 (52%), Positives = 77/112 (68%), Gaps = 1/112 (0%)
Frame = +1
Query: 1 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEP 180
VKQLIV VNKMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP
Sbjct: 145 VKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEP 202
Query: 181 STKMPWFKGWQVERKEGKADGKCLMKLSMPSCHLPA-PLTSPCVFPCKTYTK 333
+T MPW++GWQ E K G GK L++ ++ S PA P P P + K
Sbjct: 203 TTNMPWYQGWQKETKAGVVKGKTLLE-AIDSIEPPARPTDKPLRLPLQDVYK 253
Score = 41.9 bits (94), Expect = 6e-05
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = +2
Query: 494 FQRKEVSVKELRRGYVAGDSKNNPPKG 574
F K VSVK++RRG V GDSKN+PP G
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMG 334
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 64.5 bits (150), Expect = 1e-11
Identities = 38/105 (36%), Positives = 55/105 (52%), Gaps = 3/105 (2%)
Frame = +3
Query: 255 EALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPANIT 425
EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G +V +++
Sbjct: 236 EAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLK 295
Query: 426 TEVKSVEMHHEALQEAVPGDNVGFNVKKCPSRNCVVVMLLVTPKT 560
T V +EM + L AV GDN G ++ M++ P T
Sbjct: 296 TTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMIVAQPGT 340
Score = 28.3 bits (60), Expect = 0.85
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = +1
Query: 1 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 99
VKQ++V +NK+D EP E E+++ +S Y
Sbjct: 169 VKQIVVYINKVDMVEPDMIELVEMEMRELLSEY 201
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 53.6 bits (123), Expect = 2e-08
Identities = 29/72 (40%), Positives = 44/72 (61%), Gaps = 3/72 (4%)
Frame = +1
Query: 1 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY-IKKIGYNPAAVAFVPISGWHGDNML- 174
+ +++V VNK+D +SE RF+EIK VS + IK +G+ + V FVPIS G N++
Sbjct: 315 ISEIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQ 372
Query: 175 -EPSTKMPWFKG 207
+ S W+KG
Sbjct: 373 KDSSDLYKWYKG 384
Score = 45.2 bits (102), Expect = 7e-06
Identities = 29/79 (36%), Positives = 42/79 (53%), Gaps = 1/79 (1%)
Frame = +3
Query: 258 ALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVK 437
ALD ++PP +P KPLRL + DVY+ TV GRVE G ++ ++ + VK
Sbjct: 390 ALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVK 448
Query: 438 SVEMHHEALQE-AVPGDNV 491
+V + + AV GD V
Sbjct: 449 NVIRNSDPSSTWAVAGDTV 467
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 43.6 bits (98), Expect = 2e-05
Identities = 25/85 (29%), Positives = 44/85 (51%), Gaps = 1/85 (1%)
Frame = +3
Query: 255 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 434
E LD++ R + P +P+ YK +GT+ G++E G +K + V+ P N T EV
Sbjct: 453 EYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEV 510
Query: 435 KSV-EMHHEALQEAVPGDNVGFNVK 506
++ + E + ++ GD V V+
Sbjct: 511 TAIYDEADEEISSSICGDQVRLRVR 535
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 27.5 bits (58), Expect = 1.5
Identities = 15/63 (23%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = +2
Query: 53 TVSPDLRKSRRKYPHTSRRLATTQLL---SLSCPFLDGTETTCWSLQPKCLGSRDGRWSV 223
+ +PD+ ++PHT +R +T + L LSC + +E + P G +D + +
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRCLSCEYSTLSEASDSMSNPLFFGDQDNGYVI 204
Query: 224 KKA 232
++
Sbjct: 205 SQS 207
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 2.0
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -1
Query: 433 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 317
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 26.6 bits (56), Expect = 2.6
Identities = 19/72 (26%), Positives = 31/72 (43%)
Frame = +3
Query: 255 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 434
E + I PP + PLR L D + G + + R+ G +K G V+ EV
Sbjct: 228 EIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRIMEGFIKKGGKVMSVNTGRKYEV 287
Query: 435 KSVEMHHEALQE 470
+ V + + + E
Sbjct: 288 QQVGIMYPDMTE 299
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 26.2 bits (55), Expect = 3.4
Identities = 12/32 (37%), Positives = 16/32 (50%), Gaps = 1/32 (3%)
Frame = -2
Query: 309 DAGACQ-WGGQVAGWHRELHEAFSVSFAFFTL 217
D G C WG +G RE + +F FFT+
Sbjct: 287 DEGQCNPWGNSASGT-REFSKVIGAAFTFFTI 317
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 6.0
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 382 NTPVSTLPTGTVPIPPIL 329
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 25.4 bits (53), Expect = 6.0
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = +1
Query: 31 MDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 201
+D +P + F IKK+V Y+ ++ + P + G N LEP TK PW+
Sbjct: 107 LDLKKPLLPQILFGNIKKDV--YLDQV-HRPRHYRGSGSAPLFG-NFLEPLTKTPWY 159
>SPCC965.13 |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 537
Score = 25.4 bits (53), Expect = 6.0
Identities = 10/29 (34%), Positives = 19/29 (65%)
Frame = -3
Query: 536 NHDAIP*RTLLYVETYIVSRYSFLESFVV 450
NH A P + L+ + ++ + Y+FLE +V+
Sbjct: 75 NHPAHPHKWPLWKKLFVATVYTFLEVYVI 103
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 25.0 bits (52), Expect = 7.9
Identities = 13/38 (34%), Positives = 16/38 (42%)
Frame = -1
Query: 469 SWRASWCISTDLTSVVMLAGAKTTMVPGFNTPVSTLPT 356
SW SW +ST L +V + G T ST T
Sbjct: 97 SWNPSWMVSTILVGLVSFMTSDEITTGGIVTSESTRRT 134
>SPBC11B10.05c |rsp1||random septum position protein
Rsp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 494
Score = 25.0 bits (52), Expect = 7.9
Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +1
Query: 82 KEVS---SYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 207
KEVS SYI +P +G+ +N+++P + P FKG
Sbjct: 159 KEVSTSKSYISSGYLHPKTSPIFKKNGYATENVVDPISSSPRFKG 203
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,748,290
Number of Sequences: 5004
Number of extensions: 61444
Number of successful extensions: 236
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 246098644
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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