BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20536 (577 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 174 6e-46 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 174 6e-46 AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 174 6e-46 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 162 2e-42 EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-a... 51 7e-09 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 6.6 AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 6.6 AB208108-1|BAE72140.1| 92|Apis mellifera Broad complex zinc fi... 21 6.6 AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase pro... 21 8.7 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 174 bits (423), Expect = 6e-46 Identities = 78/86 (90%), Positives = 82/86 (95%) Frame = +1 Query: 1 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEP 180 VKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE Sbjct: 72 VKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEV 131 Query: 181 STKMPWFKGWQVERKEGKADGKCLMK 258 S+KMPWFKGW VERKEGK +GKCL++ Sbjct: 132 SSKMPWFKGWTVERKEGKVEGKCLIE 157 Score = 33.5 bits (73), Expect = 0.002 Identities = 14/15 (93%), Positives = 14/15 (93%) Frame = +3 Query: 255 EALDAILPPARPTDK 299 EALDAILPP RPTDK Sbjct: 157 EALDAILPPTRPTDK 171 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 174 bits (423), Expect = 6e-46 Identities = 78/86 (90%), Positives = 82/86 (95%) Frame = +1 Query: 1 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEP 180 VKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE Sbjct: 88 VKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEV 147 Query: 181 STKMPWFKGWQVERKEGKADGKCLMK 258 S+KMPWFKGW VERKEGK +GKCL++ Sbjct: 148 SSKMPWFKGWTVERKEGKVEGKCLIE 173 Score = 165 bits (400), Expect = 4e-43 Identities = 77/84 (91%), Positives = 79/84 (94%) Frame = +3 Query: 255 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 434 EALDAILPP RPTDK LRLPLQDVYKIGGIGTVPVGRVETGVLKPG +V FAPA +TTEV Sbjct: 173 EALDAILPPTRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEV 232 Query: 435 KSVEMHHEALQEAVPGDNVGFNVK 506 KSVEMHHEALQEAVPGDNVGFNVK Sbjct: 233 KSVEMHHEALQEAVPGDNVGFNVK 256 Score = 41.1 bits (92), Expect = 8e-06 Identities = 19/22 (86%), Positives = 19/22 (86%) Frame = +2 Query: 494 FQRKEVSVKELRRGYVAGDSKN 559 F K VSVKELRRGYVAGDSKN Sbjct: 253 FNVKNVSVKELRRGYVAGDSKN 274 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 174 bits (423), Expect = 6e-46 Identities = 78/86 (90%), Positives = 82/86 (95%) Frame = +1 Query: 1 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEP 180 VKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE Sbjct: 145 VKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEV 204 Query: 181 STKMPWFKGWQVERKEGKADGKCLMK 258 S+KMPWFKGW VERKEGK +GKCL++ Sbjct: 205 SSKMPWFKGWTVERKEGKVEGKCLIE 230 Score = 165 bits (400), Expect = 4e-43 Identities = 77/84 (91%), Positives = 79/84 (94%) Frame = +3 Query: 255 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 434 EALDAILPP RPTDK LRLPLQDVYKIGGIGTVPVGRVETGVLKPG +V FAPA +TTEV Sbjct: 230 EALDAILPPTRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEV 289 Query: 435 KSVEMHHEALQEAVPGDNVGFNVK 506 KSVEMHHEALQEAVPGDNVGFNVK Sbjct: 290 KSVEMHHEALQEAVPGDNVGFNVK 313 Score = 55.2 bits (127), Expect = 4e-10 Identities = 25/28 (89%), Positives = 25/28 (89%) Frame = +2 Query: 494 FQRKEVSVKELRRGYVAGDSKNNPPKGA 577 F K VSVKELRRGYVAGDSKNNPPKGA Sbjct: 310 FNVKNVSVKELRRGYVAGDSKNNPPKGA 337 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 162 bits (394), Expect = 2e-42 Identities = 72/86 (83%), Positives = 79/86 (91%) Frame = +1 Query: 1 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEP 180 VKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWHGDNMLEP Sbjct: 145 VKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEP 204 Query: 181 STKMPWFKGWQVERKEGKADGKCLMK 258 S K PW+KGW+VERK+G ADGK L++ Sbjct: 205 SPKTPWYKGWKVERKDGNADGKTLIE 230 Score = 160 bits (388), Expect = 1e-41 Identities = 74/84 (88%), Positives = 79/84 (94%) Frame = +3 Query: 255 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 434 EALDAILPP+RPTDK LRLPLQDVYKIGGIGTVPVGRVETG+LKPG +V FAPA +TTEV Sbjct: 230 EALDAILPPSRPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEV 289 Query: 435 KSVEMHHEALQEAVPGDNVGFNVK 506 KSVEMHHEAL EA+PGDNVGFNVK Sbjct: 290 KSVEMHHEALTEALPGDNVGFNVK 313 Score = 51.2 bits (117), Expect = 7e-09 Identities = 22/28 (78%), Positives = 24/28 (85%) Frame = +2 Query: 494 FQRKEVSVKELRRGYVAGDSKNNPPKGA 577 F K +SVKELRRGYVAGDSKN PP+GA Sbjct: 310 FNVKNISVKELRRGYVAGDSKNQPPRGA 337 Score = 21.8 bits (44), Expect = 5.0 Identities = 13/36 (36%), Positives = 18/36 (50%) Frame = -1 Query: 487 LSPGTASWRASWCISTDLTSVVMLAGAKTTMVPGFN 380 L PG A ++T++ SV M A T +PG N Sbjct: 272 LKPGMLVTFAPAALTTEVKSVEMHHEALTEALPGDN 307 >EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-alpha protein. Length = 119 Score = 51.2 bits (117), Expect = 7e-09 Identities = 22/24 (91%), Positives = 23/24 (95%) Frame = +3 Query: 435 KSVEMHHEALQEAVPGDNVGFNVK 506 KSVEMHHEAL EA+PGDNVGFNVK Sbjct: 1 KSVEMHHEALTEALPGDNVGFNVK 24 Score = 51.2 bits (117), Expect = 7e-09 Identities = 22/28 (78%), Positives = 24/28 (85%) Frame = +2 Query: 494 FQRKEVSVKELRRGYVAGDSKNNPPKGA 577 F K +SVKELRRGYVAGDSKN PP+GA Sbjct: 21 FNVKNISVKELRRGYVAGDSKNQPPRGA 48 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 21.4 bits (43), Expect = 6.6 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = -2 Query: 189 FG*RLQHVVSVPSRNGHESDSSWV 118 FG L H++ V +N + + WV Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58 >AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein. Length = 580 Score = 21.4 bits (43), Expect = 6.6 Identities = 7/20 (35%), Positives = 13/20 (65%) Frame = +2 Query: 32 WIPLNHHTVSPDLRKSRRKY 91 W+P+N + S +L +R+Y Sbjct: 443 WLPVNENYKSLNLAAQKREY 462 >AB208108-1|BAE72140.1| 92|Apis mellifera Broad complex zinc finger domain-Z3 isoform protein. Length = 92 Score = 21.4 bits (43), Expect = 6.6 Identities = 11/38 (28%), Positives = 16/38 (42%) Frame = -3 Query: 560 CFWSHQQHNHDAIP*RTLLYVETYIVSRYSFLESFVVH 447 C++S ++H D LYV + RY S H Sbjct: 17 CYYSLKRHFQDKHEQSDTLYVCEFCNRRYRTKNSLTTH 54 >AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase protein. Length = 588 Score = 21.0 bits (42), Expect = 8.7 Identities = 8/18 (44%), Positives = 12/18 (66%) Frame = -2 Query: 84 LLDFLKSGLTVWWFSGIH 31 L F++SG+T W S I+ Sbjct: 56 LSHFIESGITAIWLSPIN 73 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 185,353 Number of Sequences: 438 Number of extensions: 4171 Number of successful extensions: 21 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 21 length of database: 146,343 effective HSP length: 55 effective length of database: 122,253 effective search space used: 16626408 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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