Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20534
(686 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g29130.1 68417.m04169 hexokinase 1 (HXK1) identical to hexoki... 77 8e-15
At2g19860.1 68415.m02322 hexokinase 2 (HXK2) identical to hexoki... 77 1e-14
At3g20040.1 68416.m02535 hexokinase, putative similar to hexokin... 72 4e-13
At1g50460.1 68414.m05656 hexokinase, putative similar to hexokin... 71 5e-13
At1g47840.1 68414.m05325 hexokinase, putative similar to hexokin... 71 1e-12
At4g37840.1 68417.m05353 hexokinase, putative similar to hexokin... 53 2e-07
At4g02070.1 68417.m00277 DNA mismatch repair protein MSH6-1 (MSH... 29 2.2
At3g46270.1 68416.m05008 receptor protein kinase-related contain... 29 2.9
At2g28240.1 68415.m03428 hydroxyproline-rich glycoprotein family... 29 2.9
At5g35100.1 68418.m04153 peptidyl-prolyl cis-trans isomerase cyc... 27 8.8
>At4g29130.1 68417.m04169 hexokinase 1 (HXK1) identical to
hexokinase 1 [Arabidopsis thaliana] Swiss-Prot:Q42525
Length = 496
Score = 77.4 bits (182), Expect = 8e-15
Identities = 42/91 (46%), Positives = 53/91 (58%), Gaps = 5/91 (5%)
Frame = +1
Query: 10 AIPKHIMTGTGIALFDHIAECLANFMKE-----HGVYEERLALGFTFSFPLKQLGLTKGV 174
+IP H+MTG LF+ IAE LA F+ H + LGFTFSFP+KQ L+ G
Sbjct: 130 SIPPHLMTGGSDELFNFIAEALAKFVATECEDFHLPEGRQRELGFTFSFPVKQTSLSSGS 189
Query: 175 LQRWTKGFSCSGVVGEDVVQGLKDAIAEEGM 267
L +WTKGFS VG+DVV L A+ G+
Sbjct: 190 LIKWTKGFSIEEAVGQDVVGALNKALERVGL 220
Score = 54.0 bits (124), Expect = 9e-08
Identities = 27/65 (41%), Positives = 38/65 (58%), Gaps = 1/65 (1%)
Frame = +3
Query: 279 ICAILNDTTGTLMSCAWKNHNCKIGLIVGTGSNACYVEKTANCELFDG-EPDKEELLINT 455
I A++NDT GTL + N + +I+GTG+NA YVE+ + G P E++IN
Sbjct: 224 IAALVNDTVGTLAGGRYYNPDVVAAVILGTGTNAAYVERATAIPKWHGLLPKSGEMVINM 283
Query: 456 EWGAF 470
EWG F
Sbjct: 284 EWGNF 288
Score = 54.0 bits (124), Expect = 9e-08
Identities = 27/58 (46%), Positives = 34/58 (58%), Gaps = 1/58 (1%)
Frame = +2
Query: 470 WG*-RQPRLRADRVDREVDVTSINPGKQIQEKMISGMYLGELVRLALVKFTRMGLLFG 640
WG R L D +D S+NPG+QI EK+ISGMYLGE++R L+K FG
Sbjct: 285 WGNFRSSHLPLTEFDHTLDFESLNPGEQILEKIISGMYLGEILRRVLLKMAEDAAFFG 342
>At2g19860.1 68415.m02322 hexokinase 2 (HXK2) identical to
hexokinase 2 [Arabidopsis thaliana] Swiss-Prot:P93834
Length = 502
Score = 76.6 bits (180), Expect = 1e-14
Identities = 44/98 (44%), Positives = 54/98 (55%), Gaps = 5/98 (5%)
Frame = +1
Query: 10 AIPKHIMTGTGIALFDHIAECLANFMKEHGVY-----EERLALGFTFSFPLKQLGLTKGV 174
+IP H+MTG LFD I + LA F+ G + LGFTFSFP+KQL L+ G
Sbjct: 130 SIPPHLMTGKSHELFDFIVDVLAKFVATEGEDFHLPPGRQRELGFTFSFPVKQLSLSSGT 189
Query: 175 LQRWTKGFSCSGVVGEDVVQGLKDAIAEEGMYRLTYAL 288
L WTKGFS V +DVV L A+ G+ L AL
Sbjct: 190 LINWTKGFSIDDTVDKDVVGELVKAMERVGLDMLVAAL 227
Score = 56.8 bits (131), Expect = 1e-08
Identities = 28/58 (48%), Positives = 35/58 (60%), Gaps = 1/58 (1%)
Frame = +2
Query: 470 WG*-RQPRLRADRVDREVDVTSINPGKQIQEKMISGMYLGELVRLALVKFTRMGLLFG 640
WG R L D +DV S+NPG+QI EK+ISGMYLGE++R L+K FG
Sbjct: 285 WGNFRSSHLPLTEYDHSLDVDSLNPGEQILEKIISGMYLGEILRRVLLKMAEEAAFFG 342
Score = 54.4 bits (125), Expect = 7e-08
Identities = 26/65 (40%), Positives = 39/65 (60%), Gaps = 1/65 (1%)
Frame = +3
Query: 279 ICAILNDTTGTLMSCAWKNHNCKIGLIVGTGSNACYVEKTANCELFDG-EPDKEELLINT 455
+ A++NDT GTL + N + + +I+GTG+NA YVE+ + G P E++IN
Sbjct: 224 VAALVNDTIGTLAGGRYTNPDVVVAVILGTGTNAAYVERAHAIPKWHGLLPKSGEMVINM 283
Query: 456 EWGAF 470
EWG F
Sbjct: 284 EWGNF 288
>At3g20040.1 68416.m02535 hexokinase, putative similar to hexokinase
1 [Spinacia oleracea] Swiss-Prot:Q9SEK3
Length = 488
Score = 71.7 bits (168), Expect = 4e-13
Identities = 35/92 (38%), Positives = 55/92 (59%), Gaps = 5/92 (5%)
Frame = +1
Query: 7 YAIPKHIMTGTGIALFDHIAECLANFMKEHG-----VYEERLALGFTFSFPLKQLGLTKG 171
++IP +M T LFD +A L F+++ G + L FTFSFP+KQ ++ G
Sbjct: 129 HSIPTSLMNSTSEVLFDFLASSLQRFIEKEGNDFSLSQPLKRELAFTFSFPVKQTSISSG 188
Query: 172 VLQRWTKGFSCSGVVGEDVVQGLKDAIAEEGM 267
VL +WTKGF+ S + GED+ + L+ A+ + G+
Sbjct: 189 VLIKWTKGFAISEMAGEDIAECLQGALNKRGL 220
Score = 47.6 bits (108), Expect = 8e-06
Identities = 23/69 (33%), Positives = 39/69 (56%), Gaps = 1/69 (1%)
Frame = +3
Query: 267 VQIDICAILNDTTGTLMSCAWKNHNCKIGLIVGTGSNACYVEKT-ANCELFDGEPDKEEL 443
+ I + A++NDT G L + + + ++ GTGSNACY+E+T A + + +
Sbjct: 220 LDIRVAALVNDTVGALSFGHFHDPDTIAAVVFGTGSNACYLERTDAIIKCQNPRTTSGSM 279
Query: 444 LINTEWGAF 470
++N EWG F
Sbjct: 280 VVNMEWGNF 288
Score = 44.0 bits (99), Expect = 9e-05
Identities = 23/60 (38%), Positives = 33/60 (55%)
Frame = +2
Query: 461 GGVWG*RQPRLRADRVDREVDVTSINPGKQIQEKMISGMYLGELVRLALVKFTRMGLLFG 640
G W R PR D E+D S+N EKMI GMYLG++VR +++ ++ +FG
Sbjct: 286 GNFWSSRLPRTS---YDLELDAESMNSNDMGFEKMIGGMYLGDIVRRVILRMSQESDIFG 342
>At1g50460.1 68414.m05656 hexokinase, putative similar to hexokinase
1 [Spinacia oleracea] Swiss-Prot:Q9SEK3
Length = 498
Score = 71.3 bits (167), Expect = 5e-13
Identities = 36/91 (39%), Positives = 55/91 (60%), Gaps = 4/91 (4%)
Frame = +1
Query: 7 YAIPKHIMTGTGIALFDHIAECLANFMK--EHGVYEE--RLALGFTFSFPLKQLGLTKGV 174
+ IP H+M T LF+ +A L F++ E+G + R L FTFSFP+K ++ GV
Sbjct: 129 HPIPSHLMNSTSEVLFNFLAFSLERFIEKEENGSDSQGVRRELAFTFSFPVKHTSISSGV 188
Query: 175 LQRWTKGFSCSGVVGEDVVQGLKDAIAEEGM 267
L +WTKGF S +VG+D+ + L+ A+ G+
Sbjct: 189 LIKWTKGFEISEMVGQDIAECLQGALNRRGL 219
Score = 47.2 bits (107), Expect = 1e-05
Identities = 22/69 (31%), Positives = 38/69 (55%), Gaps = 1/69 (1%)
Frame = +3
Query: 267 VQIDICAILNDTTGTLMSCAWKNHNCKIGLIVGTGSNACYVEKTANCELFDG-EPDKEEL 443
+ + + A++NDT G L + + + + ++ GTGSNACY+E+T G +
Sbjct: 219 LDMHVAALVNDTVGALSLGYYHDPDTVVAVVFGTGSNACYLERTDAIIKCQGLLTTSGSM 278
Query: 444 LINTEWGAF 470
++N EWG F
Sbjct: 279 VVNMEWGNF 287
Score = 39.9 bits (89), Expect = 0.002
Identities = 23/60 (38%), Positives = 32/60 (53%)
Frame = +2
Query: 461 GGVWG*RQPRLRADRVDREVDVTSINPGKQIQEKMISGMYLGELVRLALVKFTRMGLLFG 640
G W PR D +D +D S N EKMISGMYLG++VR +++ + +FG
Sbjct: 285 GNFWSSHLPRTSYD-ID--LDAESSNANDMGFEKMISGMYLGDIVRRVILRMSEDSDIFG 341
>At1g47840.1 68414.m05325 hexokinase, putative similar to hexokinase
1 [Arabidopsis thaliana] Swiss-Prot:Q42525
Length = 493
Score = 70.5 bits (165), Expect = 1e-12
Identities = 39/92 (42%), Positives = 55/92 (59%), Gaps = 6/92 (6%)
Frame = +1
Query: 10 AIPKHIMTGTGIALFDHIAECLANFMKEHG----VYEE--RLALGFTFSFPLKQLGLTKG 171
+I + +M GT LF IA LANF+ + + EE + LGFTFSFP+KQ + G
Sbjct: 133 SISQKLMIGTSEELFGFIASKLANFVAKEKPGRFLLEEGRKRELGFTFSFPVKQTSIDSG 192
Query: 172 VLQRWTKGFSCSGVVGEDVVQGLKDAIAEEGM 267
L +WTKGF SG+ G++VV L +A+ G+
Sbjct: 193 TLSKWTKGFKVSGMEGKNVVACLNEAMEAHGL 224
Score = 56.4 bits (130), Expect = 2e-08
Identities = 24/64 (37%), Positives = 37/64 (57%)
Frame = +3
Query: 279 ICAILNDTTGTLMSCAWKNHNCKIGLIVGTGSNACYVEKTANCELFDGEPDKEELLINTE 458
+ A++ND GTL + + + +G+I+GTG+NACYVE+ + +INTE
Sbjct: 228 VSALVNDGVGTLAGARYWDEDVMVGVILGTGTNACYVEQKHAIPKLRSKSSSGTTIINTE 287
Query: 459 WGAF 470
WG F
Sbjct: 288 WGGF 291
Score = 55.2 bits (127), Expect = 4e-08
Identities = 28/57 (49%), Positives = 34/57 (59%)
Frame = +2
Query: 470 WG*RQPRLRADRVDREVDVTSINPGKQIQEKMISGMYLGELVRLALVKFTRMGLLFG 640
WG L D E+D TS+NPG+ + EKMISGMYLGE+VR L+ LFG
Sbjct: 288 WGGFSKILPQTIFDLEMDETSLNPGEHLYEKMISGMYLGEIVRRVLLHMCETSDLFG 344
>At4g37840.1 68417.m05353 hexokinase, putative similar to hexokinase
1 [Spinacia oleracea] Swiss-Prot:Q9SEK3
Length = 493
Score = 53.2 bits (122), Expect = 2e-07
Identities = 27/58 (46%), Positives = 34/58 (58%), Gaps = 1/58 (1%)
Frame = +2
Query: 470 WG*-RQPRLRADRVDREVDVTSINPGKQIQEKMISGMYLGELVRLALVKFTRMGLLFG 640
WG R L D +D S+NPG +I EKM+SG YLGE+VR L+K + LFG
Sbjct: 285 WGDFRSCHLPITEFDASLDAESLNPGHRIFEKMVSGRYLGEIVRRVLLKMSEESALFG 342
Score = 36.3 bits (80), Expect = 0.019
Identities = 16/63 (25%), Positives = 35/63 (55%), Gaps = 1/63 (1%)
Frame = +3
Query: 285 AILNDTTGTLMSCAWKNHNCKIGLIVGTGSNACYVEKTANCELFDGE-PDKEELLINTEW 461
A++++T G L + + + + +G G+NA Y+E+ + + +E++++TEW
Sbjct: 226 ALVDNTIGELAGGRYYHKDTVAAVSLGMGTNAAYIEQAQEISRWKSAIREPQEIVVSTEW 285
Query: 462 GAF 470
G F
Sbjct: 286 GDF 288
>At4g02070.1 68417.m00277 DNA mismatch repair protein MSH6-1
(MSH6-1) (AGAA.3) identical to SP|O04716 DNA mismatch
repair protein MSH6-1 (AtMsh6-1) cress] {Arabidopsis
thaliana}
Length = 1324
Score = 29.5 bits (63), Expect = 2.2
Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 4/72 (5%)
Frame = -1
Query: 299 IIQNSAYVNLYIPSSAIASLRPCTTSSPTTPEQEKPLVHLCN----TPFVRPSCFSGKLN 132
++ + + SA +S P + SP+ ++ P + N +P P + KLN
Sbjct: 14 LVNQQRQITSFFGKSASSSSSPSPSPSPSLSNKKTPKSNNPNPKSPSPSPSPPKKTPKLN 73
Query: 131 VNPSANLSS*TP 96
NPS+NL + +P
Sbjct: 74 PNPSSNLPARSP 85
>At3g46270.1 68416.m05008 receptor protein kinase-related contains
weak similarity to light repressible receptor protein
kinase (GI:1321686) [Arabidopsis thaliana]
Length = 470
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/50 (28%), Positives = 24/50 (48%)
Frame = -1
Query: 392 LDVTSVAASTNDESYFAVVVFPCAGHQCTRCIIQNSAYVNLYIPSSAIAS 243
+D+T D YF+ V+F A + C+++ S N +I S + S
Sbjct: 128 VDITESLLDDEDTFYFSEVIFAPASENISVCLLRTSPSDNPFISSIEVYS 177
>At2g28240.1 68415.m03428 hydroxyproline-rich glycoprotein family
protein
Length = 660
Score = 29.1 bits (62), Expect = 2.9
Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = -1
Query: 263 PSSAIASLRPCTTSSPTTPEQ-EKPLVHLCNTPFVRPSCFSGKLNVNPSANLSS 105
P ++ A RP T++ TP PL H CNTP +PS S + V + + S
Sbjct: 341 PMNSTAPPRPSVTAAEATPPNLSAPLPH-CNTP--QPSPISQQAAVESNTQMQS 391
>At5g35100.1 68418.m04153 peptidyl-prolyl cis-trans isomerase
cyclophilin-type family protein contains Pfam domain,
PF00160: peptidyl-prolyl cis-trans isomerase,
cyclophilin-type
Length = 290
Score = 27.5 bits (58), Expect = 8.8
Identities = 18/57 (31%), Positives = 29/57 (50%)
Frame = -1
Query: 260 SSAIASLRPCTTSSPTTPEQEKPLVHLCNTPFVRPSCFSGKLNVNPSANLSS*TPCS 90
S+ IA++ P + P +P+ + + + F+ S +PSA LSS TPCS
Sbjct: 29 STTIATVTPPPPAKPPSPD-----ITITDRVFLDFSLCPTYFRSDPSATLSSTTPCS 80
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,750,375
Number of Sequences: 28952
Number of extensions: 338185
Number of successful extensions: 928
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 886
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 925
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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