BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20523 (592 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g17470.1 68414.m02143 developmentally regulated GTP-binding p... 108 3e-24 At1g72660.1 68414.m08403 developmentally regulated GTP-binding p... 107 7e-24 At4g39520.1 68417.m05588 GTP-binding protein, putative similar t... 103 9e-23 At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative 29 2.3 At5g43060.1 68418.m05256 cysteine proteinase, putative / thiol p... 28 4.1 At4g31630.1 68417.m04493 transcriptional factor B3 family protei... 28 5.4 At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-... 28 5.4 At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-... 28 5.4 At1g67170.1 68414.m07641 expressed protein similar to enterophil... 28 5.4 At5g61600.1 68418.m07729 ethylene-responsive element-binding fam... 27 7.1 At5g24450.1 68418.m02882 transcription factor-related low simila... 27 7.1 At3g19250.1 68416.m02442 hypothetical protein 27 7.1 At5g58940.1 68418.m07383 protein kinase family protein contains ... 27 9.4 At4g27040.1 68417.m03888 expressed protein 27 9.4 >At1g17470.1 68414.m02143 developmentally regulated GTP-binding protein (DRG1) identical to developmentally regulated GTP binding protein (DRG1) [Arabidopsis thaliana] GI:2345150 Length = 399 Score = 108 bits (259), Expect = 3e-24 Identities = 60/112 (53%), Positives = 66/112 (58%) Frame = +3 Query: 255 ARTQKNKATEYHLGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVALIGFPSVG 434 ARTQKNKATEYHLG LKAK+AK R+QLLEP K GEGF+V K G RVALIGFPSVG Sbjct: 15 ARTQKNKATEYHLGQLKAKIAKLRTQLLEPPKGASGGGEGFEVTKYGHGRVALIGFPSVG 74 Query: 435 KXXXXXXXXXXXXEAASXXXXXXXXXXXXXNIAVPTYNC*DLPGIIEGAAQG 590 K EAAS + DLPGIIEGA++G Sbjct: 75 KSTLLTMLTGTHSEAASYEFTTLTCIPGVIHYNDTKIQLLDLPGIIEGASEG 126 >At1g72660.1 68414.m08403 developmentally regulated GTP-binding protein, putative very strong similarity to developmentally regulated GTP binding protein (DRG1) [Arabidopsis thaliana] GI:2345150 Length = 399 Score = 107 bits (256), Expect = 7e-24 Identities = 59/112 (52%), Positives = 66/112 (58%) Frame = +3 Query: 255 ARTQKNKATEYHLGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVALIGFPSVG 434 ARTQKNKATEYHLG LKAK+AK R+QLLEP K G+GF+V K G RVALIGFPSVG Sbjct: 15 ARTQKNKATEYHLGQLKAKIAKLRTQLLEPPKGSSGGGDGFEVTKYGHGRVALIGFPSVG 74 Query: 435 KXXXXXXXXXXXXEAASXXXXXXXXXXXXXNIAVPTYNC*DLPGIIEGAAQG 590 K EAAS + DLPGIIEGA++G Sbjct: 75 KSTLLTMLTGTHSEAASYEFTTLTCIPGVIHYNDTKIQLLDLPGIIEGASEG 126 >At4g39520.1 68417.m05588 GTP-binding protein, putative similar to SP|Q9Y295 Developmentally regulated GTP-binding protein 1 (DRG 1) {Homo sapiens}; contains Pfam profiles PF02824: TGS domain, PF01018: GTP1/OBG family Length = 369 Score = 103 bits (247), Expect = 9e-23 Identities = 59/114 (51%), Positives = 63/114 (55%), Gaps = 2/114 (1%) Frame = +3 Query: 255 ARTQKNKATEYHLGLLKAKLAKYRSQLLEPSKK--GGDKGEGFDVLKSGDARVALIGFPS 428 A+TQKNKAT +HLGLLKAKLAK R LL P K GG GEGFDV KSGD+RV L+GFPS Sbjct: 16 AKTQKNKATSHHLGLLKAKLAKLRRDLLAPPTKGGGGGAGEGFDVTKSGDSRVGLVGFPS 75 Query: 429 VGKXXXXXXXXXXXXEAASXXXXXXXXXXXXXNIAVPTYNC*DLPGIIEGAAQG 590 VGK E AS DLPGIIEGA G Sbjct: 76 VGKSTLLNKLTGTFSEVASYEFTTLTCIPGVITYRGAKIQLLDLPGIIEGAKDG 129 >At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative Length = 953 Score = 29.1 bits (62), Expect = 2.3 Identities = 14/47 (29%), Positives = 22/47 (46%) Frame = +3 Query: 273 KATEYHLGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVAL 413 +A L ++ K KY LL K DK E + +SG+ ++ L Sbjct: 781 RAASAELEIVTKKFPKYNCGLLHGRMKSDDKEEALNKFRSGETQILL 827 >At5g43060.1 68418.m05256 cysteine proteinase, putative / thiol protease, putative similar to cysteine proteinase RD21A precursor (thiol protease) GI:435619, SP:P43297 from [Arabidopsis thaliana] Length = 463 Score = 28.3 bits (60), Expect = 4.1 Identities = 14/35 (40%), Positives = 19/35 (54%) Frame = -1 Query: 418 PIKATRASPDFSTSKPSPLSPPFFEGSSNCDLYFA 314 PIK + P+ S PSP+ PP + CD YF+ Sbjct: 352 PIKKGQNPPNPGPSPPSPIKPP-----TTCDKYFS 381 >At4g31630.1 68417.m04493 transcriptional factor B3 family protein similar to reproductive meristem gene 1 from [Brassica oleracea var. botrytis] GI:3170424, [Arabidopsis thaliana] GI:13604227; contains Pfam profile PF02362: B3 DNA binding domain Length = 512 Score = 27.9 bits (59), Expect = 5.4 Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 1/34 (2%) Frame = -1 Query: 457 RVDKRVDLPTEGKPIKATRASP-DFSTSKPSPLS 359 RV+KR + EG P ++TRA P + +P +S Sbjct: 435 RVEKRAQVTEEGGPSRSTRADPGNLQQKQPCSIS 468 >At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-coat protein, putative / beta-COP, putative similar to Coatomer beta subunit (Beta-coat protein) (Beta-COP) from {Rattus norvegicus} SP|P23514, {Mus musculus} SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam profile: PF01602 Adaptin N terminal region Length = 948 Score = 27.9 bits (59), Expect = 5.4 Identities = 15/48 (31%), Positives = 26/48 (54%) Frame = -2 Query: 447 KGLTYPQKESLSKQLEHHQTLVHRNLHLCLHLFLKVPATVIYILLIWL 304 K + Q L K E+ Q L+ +H C F +V +TV+++L+ +L Sbjct: 358 KEVVKTQSGELEKNGEYRQMLIQA-IHACAVKFPEVASTVVHLLMDFL 404 >At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-coat protein, putative / beta-COP, putative similar to Coatomer beta subunit (Beta-coat protein) (Beta-COP) from {Rattus norvegicus} SP|P23514, {Mus musculus} SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam profile: PF01602 Adaptin N terminal region Length = 971 Score = 27.9 bits (59), Expect = 5.4 Identities = 15/48 (31%), Positives = 26/48 (54%) Frame = -2 Query: 447 KGLTYPQKESLSKQLEHHQTLVHRNLHLCLHLFLKVPATVIYILLIWL 304 K + Q L K E+ Q L+ +H C F +V +TV+++L+ +L Sbjct: 381 KEVVKTQSGELEKNGEYRQMLIQA-IHACAVKFPEVASTVVHLLMDFL 427 >At1g67170.1 68414.m07641 expressed protein similar to enterophilin-2L (GI:12718845) [Cavia porcellus]; similar to Hyaluronan mediated motility receptor (Intracellular hyaluronic acid binding protein) (Receptor for hyaluronan-mediated motility) (CD168 antigen) (Swiss-Prot:O75330) [Homo sapiens] Length = 359 Score = 27.9 bits (59), Expect = 5.4 Identities = 12/44 (27%), Positives = 27/44 (61%), Gaps = 2/44 (4%) Frame = +1 Query: 151 AEKHYQLIDCVVNSCQITR--RIMGILEKISEIEKEMREHRKIK 276 A+ Q++ + S + R R+MG+ EK++++E E+++ +K Sbjct: 93 AQHEIQMLHAQIGSMKSEREQRMMGLAEKVAKMETELQKSEAVK 136 >At5g61600.1 68418.m07729 ethylene-responsive element-binding family protein contains similarity to ethylene responsive element binding factor 5 (AtERF5) (Swiss-Prot:O80341) [Arabidopsis thaliana]; contains Pfam PF00847: AP2 domain Length = 241 Score = 27.5 bits (58), Expect = 7.1 Identities = 9/33 (27%), Positives = 21/33 (63%) Frame = +3 Query: 258 RTQKNKATEYHLGLLKAKLAKYRSQLLEPSKKG 356 +T+K + ++ G+ + KY +++ +P+KKG Sbjct: 78 KTEKEEEERHYRGVRRRPWGKYAAEIRDPNKKG 110 >At5g24450.1 68418.m02882 transcription factor-related low similarity to transcription factor IIIC63 [Homo sapiens] GI:5281316 Length = 545 Score = 27.5 bits (58), Expect = 7.1 Identities = 12/38 (31%), Positives = 20/38 (52%) Frame = +1 Query: 433 VSQPFYRPSLTHIQKQQVMNSQR*HDTWSYRISRCQHT 546 + + F R T IQK + SQR H + + +C++T Sbjct: 414 IQEEFERSEKTRIQKDALQPSQRNHQETTKDMKKCKNT 451 >At3g19250.1 68416.m02442 hypothetical protein Length = 398 Score = 27.5 bits (58), Expect = 7.1 Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Frame = +2 Query: 440 NPFIDPHSHTFRSSKL*IHN-ADMIPGVIEYRGANIQLLRFA 562 NPF P SH+F+ ++L + AD + I + ++LL A Sbjct: 204 NPFASPESHSFQDTQLCFYQLADKLDTRIRKSKSRVRLLHHA 245 >At5g58940.1 68418.m07383 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 466 Score = 27.1 bits (57), Expect = 9.4 Identities = 17/39 (43%), Positives = 22/39 (56%), Gaps = 1/39 (2%) Frame = +1 Query: 148 VAEKHYQLIDCVVNSCQITRRIM-GILEKISEIEKEMRE 261 VAEK +L V + TR M GI EK+ I +EM+E Sbjct: 388 VAEKMLRLASECVTPTRATRPAMKGIAEKLWAIRREMKE 426 >At4g27040.1 68417.m03888 expressed protein Length = 250 Score = 27.1 bits (57), Expect = 9.4 Identities = 13/48 (27%), Positives = 25/48 (52%) Frame = +1 Query: 148 VAEKHYQLIDCVVNSCQITRRIMGILEKISEIEKEMREHRKIKRLNIT 291 + + +Y+L ++ C +TR G L + E+ +R+ RK R +T Sbjct: 92 IGDFYYELGVQIIEVCMLTRSHNGGLISLQELCNHLRQRRKKDREAVT 139 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,085,693 Number of Sequences: 28952 Number of extensions: 204938 Number of successful extensions: 622 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 584 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 614 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1171109464 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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