Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20522
         (637 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC23G7.05 |sui1|psu1|translation initiation factor eIF1|Schizo...    68   1e-12
SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizos...    28   0.98 
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    28   0.98 
SPBC16C6.05 |||translation initiation factor |Schizosaccharomyce...    27   1.7  
SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr 1...    26   5.2  
SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit L2|Sc...    25   9.1  
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar...    25   9.1  

>SPBC23G7.05 |sui1|psu1|translation initiation factor
           eIF1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 109

 Score = 68.1 bits (159), Expect = 1e-12
 Identities = 29/39 (74%), Positives = 35/39 (89%)
 Frame = +1

Query: 262 VHVRIQQRNGRKTLTTVQGLSSEYDLKKIVRACKKEFAC 378
           +H+RIQQRNGRKTLTTVQGL  E+D K+I+RA KK+FAC
Sbjct: 27  IHIRIQQRNGRKTLTTVQGLPREFDQKRILRALKKDFAC 65



 Score = 47.2 bits (107), Expect = 2e-06
 Identities = 19/43 (44%), Positives = 32/43 (74%)
 Frame = +3

Query: 381 GTVVEHPEYGEVLQLQGDQRENICQWLTKSGLVKPEQLKVHGF 509
           GT+V+  + GEV+QLQGDQR  + ++LT+   ++ + +K+HGF
Sbjct: 67  GTIVKDDDLGEVIQLQGDQRIKVMEFLTQQLGLQKKNIKIHGF 109



 Score = 26.2 bits (55), Expect = 4.0
 Identities = 10/13 (76%), Positives = 11/13 (84%)
 Frame = +2

Query: 185 SIQNLNTFDPFAD 223
           +IQN NT DPFAD
Sbjct: 3   AIQNFNTVDPFAD 15


>SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 790

 Score = 28.3 bits (60), Expect = 0.98
 Identities = 12/32 (37%), Positives = 21/32 (65%)
 Frame = -1

Query: 244 VLRAFDSIGEWVECVEILDGHTIERRVSLFQR 149
           +LR   S+  W+  ++  +G T+E+RV LF+R
Sbjct: 25  LLRDPYSLKSWLRYIKTHEGSTLEKRVLLFER 56


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 28.3 bits (60), Expect = 0.98
 Identities = 16/39 (41%), Positives = 19/39 (48%)
 Frame = -1

Query: 424 SCSTSPYSGCSTTVPCTRTPSCMPARSSSGHIPRKGLAP 308
           SCS  PY   S T    RTPS +   SS    P+  L+P
Sbjct: 205 SCSPEPYLPLSPTRSPARTPSPIRLYSSDALRPQSPLSP 243


>SPBC16C6.05 |||translation initiation factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 190

 Score = 27.5 bits (58), Expect = 1.7
 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
 Frame = +1

Query: 244 RRSSGLVHVRIQQRNGRKTLTTVQGLSS-EYDLKKIVRACKKEFA 375
           +R +  V ++  +R  RK +TTVQGL +   + KK  +    +FA
Sbjct: 93  KRMASKVLIKTIERTKRKRVTTVQGLDAFGIETKKAAKMLANKFA 137


>SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 455

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 6/64 (9%)
 Frame = +3

Query: 300 ADYGARPFLGI*PEEDRAGMQEGVRVQGTVVEH-PEYGEVL-----QLQGDQRENICQWL 461
           A   A P   +     RAG +  V V G++VEH P + ++L     +L GD  + I   +
Sbjct: 372 ARMSAVPIASLYLSTGRAGKKSDVGVDGSLVEHYPHFVDMLREALRELIGDNEKLISIGI 431

Query: 462 TKSG 473
            K G
Sbjct: 432 AKDG 435


>SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit
           L2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 318

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 17/46 (36%), Positives = 24/46 (52%)
 Frame = +1

Query: 160 KRPYVQSYVHPESQHIRPIRRCYQKLGGRRSSGLVHVRIQQRNGRK 297
           KRP V  Y+  + +  RP+    +K GGR  +G + VR  Q  G K
Sbjct: 25  KRP-VSDYLW-KGKPFRPLTVAKRKKGGRNETGRITVR-HQGGGHK 67


>SPAC823.11 |||sphingosine-1-phosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 411

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 11/25 (44%), Positives = 14/25 (56%)
 Frame = +3

Query: 198 STHSTHSPMLSKARRTTFKWFSPRP 272
           S+ ST  P+LS      F WF P+P
Sbjct: 229 SSSSTSVPILSVVLALFFIWFHPQP 253


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,541,134
Number of Sequences: 5004
Number of extensions: 49359
Number of successful extensions: 138
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)