SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20518
         (407 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g63840.1 68418.m08014 alpha-glucosidase, putative similar to ...    58   3e-09
At1g19430.1 68414.m02421 dehydration-responsive protein-related ...    29   1.2  
At1g08010.1 68414.m00874 zinc finger (GATA type) family protein ...    29   1.2  
At2g42250.1 68415.m05230 cytochrome P450 family protein similar ...    29   1.6  
At5g07530.1 68418.m00862 glycine-rich protein (GRP17) olesin; gl...    28   2.8  
At5g02050.1 68418.m00126 mitochondrial glycoprotein family prote...    28   2.8  
At5g01030.2 68418.m00006 expressed protein                             28   2.8  
At5g01030.1 68418.m00005 expressed protein                             28   2.8  
At1g03790.1 68414.m00360 zinc finger (CCCH-type) family protein ...    28   2.8  
At5g55430.1 68418.m06905 hypothetical protein                          27   3.7  
At3g29075.1 68416.m03637 glycine-rich protein                          27   3.7  
At3g22440.1 68416.m02836 hydroxyproline-rich glycoprotein family...    27   3.7  
At5g60820.1 68418.m07630 zinc finger (C3HC4-type RING finger) fa...    27   4.8  
At5g20420.1 68418.m02428 SNF2 domain-containing protein / helica...    27   4.8  
At5g04420.1 68418.m00435 kelch repeat-containing protein low sim...    27   4.8  
At4g04635.1 68417.m00678 hypothetical protein                          27   4.8  
At3g23900.1 68416.m03003 RNA recognition motif (RRM)-containing ...    27   4.8  
At5g60640.2 68418.m07611 thioredoxin family protein similar to p...    27   6.4  
At5g60640.1 68418.m07610 thioredoxin family protein similar to p...    27   6.4  
At5g55310.1 68418.m06893 DNA topoisomerase I, putative similar t...    27   6.4  
At4g30935.1 68417.m04392 WRKY family transcription factor contai...    27   6.4  
At4g21190.1 68417.m03064 pentatricopeptide (PPR) repeat-containi...    27   6.4  
At1g16760.1 68414.m02013 protein kinase family protein contains ...    27   6.4  
At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nic...    26   8.5  
At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase fa...    26   8.5  
At5g14120.1 68418.m01652 nodulin family protein similar to nodul...    26   8.5  
At1g20760.1 68414.m02600 calcium-binding EF hand family protein ...    26   8.5  

>At5g63840.1 68418.m08014 alpha-glucosidase, putative similar to
           alpha-glucosidase GI:2648032 from [Solanum tuberosum]
          Length = 921

 Score = 57.6 bits (133), Expect = 3e-09
 Identities = 31/70 (44%), Positives = 39/70 (55%), Gaps = 5/70 (7%)
 Frame = +2

Query: 200 GRNLE*DLPVPSRLQTSGHEAVSLDVVFPDADHVYGIPEHTDGLALKTT-----TSGDPY 364
           G N E      +  + SG +++S DV F D+  VYGIPEH    ALK T        +PY
Sbjct: 193 GDNWEEKFRTHTDSRPSGPQSISFDVSFYDSSFVYGIPEHATSFALKPTKGPGVEESEPY 252

Query: 365 RLYNLDVFAY 394
           RL+NLDVF Y
Sbjct: 253 RLFNLDVFEY 262



 Score = 27.5 bits (58), Expect = 3.7
 Identities = 18/72 (25%), Positives = 31/72 (43%), Gaps = 5/72 (6%)
 Frame = +3

Query: 81  NGEIAVVLNENSQLLVEPLRARREKVEDEDGDGANQLDD-----EEGTWSETFQSHHDSK 245
           +G  AVV ++  ++ V      R +V   +  G    +      E   W E F++H DS+
Sbjct: 148 DGYEAVVRHDPFEVYVREKSGDRRRVVSLNSHGLFDFEQLGRKTEGDNWEEKFRTHTDSR 207

Query: 246 PRGTKQFRSTLS 281
           P G +     +S
Sbjct: 208 PSGPQSISFDVS 219


>At1g19430.1 68414.m02421 dehydration-responsive protein-related low
           similarity to early-responsive to dehydration stress
           ERD3 protein [Arabidopsis thaliana] GI:15320410;
           contains Pfam profile PF03141: Putative
           methyltransferase
          Length = 724

 Score = 29.1 bits (62), Expect = 1.2
 Identities = 13/36 (36%), Positives = 20/36 (55%)
 Frame = +3

Query: 150 EKVEDEDGDGANQLDDEEGTWSETFQSHHDSKPRGT 257
           E+ E +D D +N+ D EEGT S+  +   D    G+
Sbjct: 143 EEAESDDSDQSNKEDGEEGTESDGNEGESDGNGDGS 178


>At1g08010.1 68414.m00874 zinc finger (GATA type) family protein
           similar to PIR:T05288 from [Arabidopsis thaliana]
          Length = 303

 Score = 29.1 bits (62), Expect = 1.2
 Identities = 13/32 (40%), Positives = 19/32 (59%)
 Frame = -1

Query: 179 PITVFVLYLFPSRP*RFHEQLRVLVENDCYFS 84
           P T+ + YLFPS P +  +      E++CYFS
Sbjct: 157 PTTLRLSYLFPSEPRKPEKSTPGKPESECYFS 188


>At2g42250.1 68415.m05230 cytochrome P450 family protein similar to
           cytochrome P450 93A1 (SP:Q42798) [Glycine max]
          Length = 514

 Score = 28.7 bits (61), Expect = 1.6
 Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
 Frame = +3

Query: 54  PLKLEFLDQNGEIAVVLNENSQLLVEPLRARRE-KVEDEDGDGANQLD 194
           PLK+     NG+  V + E   LLVE +   RE K + +DG   + LD
Sbjct: 233 PLKVMDFSGNGKKLVAVMEKYDLLVERIMKEREAKAKKKDGTRKDILD 280


>At5g07530.1 68418.m00862 glycine-rich protein (GRP17) olesin;
           glycine-rich protein 17 (GRP17) PMID:11431566; function:
           pollen recognition (PMID:10655594)
          Length = 543

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 8/18 (44%), Positives = 15/18 (83%)
 Frame = +1

Query: 169 TVMGLISWMMRKELGVRP 222
           T +GLI W++++ +GV+P
Sbjct: 130 TALGLIMWLVKRRMGVKP 147


>At5g02050.1 68418.m00126 mitochondrial glycoprotein family protein
           / MAM33 family protein low similarity to SUAPRGA1
           [Emericella nidulans] GI:6562379; contains Pfam profile
           PF02330: Mitochondrial glycoprotein
          Length = 267

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 16/56 (28%), Positives = 31/56 (55%), Gaps = 5/56 (8%)
 Frame = +3

Query: 51  EPLKLEFLDQNGEIAVVL-----NENSQLLVEPLRARREKVEDEDGDGANQLDDEE 203
           E    E +D +GE  V+L     +E  Q+ V+ + +  ++ E+E+ +  N+ DDE+
Sbjct: 101 EGFPFEIIDNSGERTVLLTRKFEDETIQVEVDSVASYDDEDEEEEAEPNNEEDDED 156


>At5g01030.2 68418.m00006 expressed protein 
          Length = 744

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 21/54 (38%), Positives = 26/54 (48%)
 Frame = +3

Query: 147 REKVEDEDGDGANQLDDEEGTWSETFQSHHDSKPRGTKQFRSTLSSLMRITSTV 308
           +E VE  DGD     D E G  SE  +  +    R  KQ RSTLS   +I+  V
Sbjct: 227 KECVEKLDGDEKIIGDSEAGLTSEKQEFSNIFLLRSRKQSRSTLSGEPQISREV 280


>At5g01030.1 68418.m00005 expressed protein 
          Length = 744

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 21/54 (38%), Positives = 26/54 (48%)
 Frame = +3

Query: 147 REKVEDEDGDGANQLDDEEGTWSETFQSHHDSKPRGTKQFRSTLSSLMRITSTV 308
           +E VE  DGD     D E G  SE  +  +    R  KQ RSTLS   +I+  V
Sbjct: 227 KECVEKLDGDEKIIGDSEAGLTSEKQEFSNIFLLRSRKQSRSTLSGEPQISREV 280


>At1g03790.1 68414.m00360 zinc finger (CCCH-type) family protein
           contains Pfam domain, PF00642: Zinc finger
           C-x8-C-x5-C-x3-H type (and similar)
          Length = 393

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 14/39 (35%), Positives = 21/39 (53%)
 Frame = +1

Query: 163 TKTVMGLISWMMRKELGVRPSSPITTPNLGARSSFARRC 279
           ++ V  L S  + + L    SSP+TTP   A ++FA  C
Sbjct: 272 SELVNSLDSMSLAEALQASSSSPVTTPVSAAAAAFASSC 310


>At5g55430.1 68418.m06905 hypothetical protein 
          Length = 149

 Score = 27.5 bits (58), Expect = 3.7
 Identities = 14/40 (35%), Positives = 23/40 (57%)
 Frame = +3

Query: 96  VVLNENSQLLVEPLRARREKVEDEDGDGANQLDDEEGTWS 215
           V L+++  L  E      E+VE+E+ +   + +DEEG WS
Sbjct: 10  VPLSQDDDLEEEEDEKEEEEVEEEEEEEEEE-EDEEGEWS 48


>At3g29075.1 68416.m03637 glycine-rich protein 
          Length = 294

 Score = 27.5 bits (58), Expect = 3.7
 Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 2/36 (5%)
 Frame = +3

Query: 138 RARREKVEDEDGDGANQLDDE--EGTWSETFQSHHD 239
           +  +EK +D+  DG N  DDE  +    E ++ HHD
Sbjct: 163 KKEKEKKKDKKKDGNNSEDDEFKKKKKKEQYKEHHD 198


>At3g22440.1 68416.m02836 hydroxyproline-rich glycoprotein family
           protein identical to hydroxyproline-rich glycoprotein
           [Arabidopsis thaliana] gi|9293881|dbj|BAB01784
          Length = 532

 Score = 27.5 bits (58), Expect = 3.7
 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
 Frame = +3

Query: 72  LDQNGEI-AVVLNENSQLLVEPLRARREKVEDEDGDGANQLDDEEGTWS 215
           +D + EI A  + E ++  +E L   R+   D   D +  +DDEEG  S
Sbjct: 81  IDHSVEIVAGKVGERARAALESLEKARDGCGDGSNDDSGDVDDEEGLLS 129


>At5g60820.1 68418.m07630 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam profile: PF00097 zinc
           finger, C3HC4 type (RING finger)
          Length = 419

 Score = 27.1 bits (57), Expect = 4.8
 Identities = 12/26 (46%), Positives = 15/26 (57%)
 Frame = +2

Query: 281 FPDADHVYGIPEHTDGLALKTTTSGD 358
           F D+D V  + EH DG+ L    SGD
Sbjct: 135 FVDSDLVVNLNEHVDGMRLLGIESGD 160


>At5g20420.1 68418.m02428 SNF2 domain-containing protein / helicase
           domain-containing protein low similarity to SP|Q9U7E0
           Transcriptional regulator ATRX homolog {Caenorhabditis
           elegans}; contains PFam profiles PF00271: Helicase
           conserved C-terminal domain, PF00176: SNF2 family
           N-terminal domain
          Length = 1261

 Score = 27.1 bits (57), Expect = 4.8
 Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 3/46 (6%)
 Frame = -2

Query: 397 YVCEDVQVVQPVRVAGRGRLQGQTVRVLRYTVDV---IRIREDNVE 269
           YV ED++  Q +R+  R       +  LR  +DV    RI ED++E
Sbjct: 48  YVLEDIRPFQRLRLRSRKAALSDCICFLRPDIDVCVLYRIHEDDLE 93


>At5g04420.1 68418.m00435 kelch repeat-containing protein low
           similarity to rngB protein, Dictyostelium discoideum,
           PIR:S68824; contains Pfam profile PF01344: Kelch motif
          Length = 514

 Score = 27.1 bits (57), Expect = 4.8
 Identities = 19/67 (28%), Positives = 29/67 (43%)
 Frame = +3

Query: 12  LVNTQGHKVIITSEPLKLEFLDQNGEIAVVLNENSQLLVEPLRARREKVEDEDGDGANQL 191
           L + +G  +   S   KLE      + A+   ++ +  VE LR R+    DE+ DG  Q 
Sbjct: 444 LQSVEGQLISERSRCFKLEAQIAELQKALESGQSIEAEVEMLR-RQRSASDEEEDGTVQR 502

Query: 192 DDEEGTW 212
               G W
Sbjct: 503 QGSAGVW 509


>At4g04635.1 68417.m00678 hypothetical protein
          Length = 284

 Score = 27.1 bits (57), Expect = 4.8
 Identities = 12/46 (26%), Positives = 25/46 (54%)
 Frame = +3

Query: 150 EKVEDEDGDGANQLDDEEGTWSETFQSHHDSKPRGTKQFRSTLSSL 287
           E+ E+E+G+G  + ++EE    E  + H   KP G +   +  +++
Sbjct: 228 EEGEEEEGEGEEEEEEEEEGEEEEGEEHVAYKPIGDRAMEALRNNI 273


>At3g23900.1 68416.m03003 RNA recognition motif (RRM)-containing
           protein 
          Length = 987

 Score = 27.1 bits (57), Expect = 4.8
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = +2

Query: 5   SDSRQHAGA*SHHNIRTSQTRIPGPEWRNSSRSQRELS 118
           S+SR++  +  HH+  + ++R   P+ R S +   ELS
Sbjct: 574 SESRRYGRSDEHHSSSSRRSRSVSPKKRKSGQEDSELS 611


>At5g60640.2 68418.m07611 thioredoxin family protein similar to
           protein disulfide isomerase GI:5902592 from [Volvox
           carteri f. nagariensis], GI:2708314 from Chlamydomonas
           reinhardtii; contains Pfam profile: PF00085 Thioredoxin
          Length = 536

 Score = 26.6 bits (56), Expect = 6.4
 Identities = 13/30 (43%), Positives = 16/30 (53%)
 Frame = +3

Query: 159 EDEDGDGANQLDDEEGTWSETFQSHHDSKP 248
           E+ED D  N  DDEEG +S+      D  P
Sbjct: 67  EEEDPDMYNDDDDEEGDFSDLGNPDSDPLP 96


>At5g60640.1 68418.m07610 thioredoxin family protein similar to
           protein disulfide isomerase GI:5902592 from [Volvox
           carteri f. nagariensis], GI:2708314 from Chlamydomonas
           reinhardtii; contains Pfam profile: PF00085 Thioredoxin
          Length = 597

 Score = 26.6 bits (56), Expect = 6.4
 Identities = 13/30 (43%), Positives = 16/30 (53%)
 Frame = +3

Query: 159 EDEDGDGANQLDDEEGTWSETFQSHHDSKP 248
           E+ED D  N  DDEEG +S+      D  P
Sbjct: 67  EEEDPDMYNDDDDEEGDFSDLGNPDSDPLP 96


>At5g55310.1 68418.m06893 DNA topoisomerase I, putative similar to
           Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis
           thaliana]
          Length = 917

 Score = 26.6 bits (56), Expect = 6.4
 Identities = 19/79 (24%), Positives = 38/79 (48%), Gaps = 3/79 (3%)
 Frame = +3

Query: 48  SEPLKLEFLDQNGEIAVVLNENSQLL--VEPLRARREKVEDEDGDGANQLDDEEGTWSET 221
           SEP +   ++Q   I+   +   + L   +PLRA +  V++++ DG + +       S++
Sbjct: 258 SEPARPVKVEQGSHISATQDAKGKNLDASKPLRANQATVKEDNSDGDDHVPIASRMKSDS 317

Query: 222 FQSHHDS-KPRGTKQFRST 275
             +   S KP  +K   S+
Sbjct: 318 SNNKSSSAKPSSSKMIASS 336


>At4g30935.1 68417.m04392 WRKY family transcription factor contains
           Pfam profile: PF03106 WRKY DNA -binding domain
          Length = 466

 Score = 26.6 bits (56), Expect = 6.4
 Identities = 15/38 (39%), Positives = 19/38 (50%)
 Frame = +3

Query: 144 RREKVEDEDGDGANQLDDEEGTWSETFQSHHDSKPRGT 257
           + EKVE E  DG +Q  DEE +     +  HD   R T
Sbjct: 17  KSEKVEPEK-DGLSQFRDEEKSLGADMEDLHDETVRET 53


>At4g21190.1 68417.m03064 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 307

 Score = 26.6 bits (56), Expect = 6.4
 Identities = 9/23 (39%), Positives = 14/23 (60%)
 Frame = +3

Query: 183 NQLDDEEGTWSETFQSHHDSKPR 251
           N+LD+ E  W++ F  H +  PR
Sbjct: 140 NRLDEAEELWNKLFMEHLEGTPR 162


>At1g16760.1 68414.m02013 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 758

 Score = 26.6 bits (56), Expect = 6.4
 Identities = 18/50 (36%), Positives = 21/50 (42%)
 Frame = +2

Query: 218 DLPVPSRLQTSGHEAVSLDVVFPDADHVYGIPEHTDGLALKTTTSGDPYR 367
           DL V      S  E   LDVV  D D V  I E+    A++    G P R
Sbjct: 77  DLFVSFHCYCSRKEIHCLDVVLEDVDKVKAIVEYVTVSAIENLVLGAPSR 126


>At5g40340.1 68418.m04894 PWWP domain-containing protein KED,
           Nicotiana tabacum, EMBL:AB009883
          Length = 1008

 Score = 26.2 bits (55), Expect = 8.5
 Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 9/84 (10%)
 Frame = +3

Query: 9   ILVNTQGHK---VIITSEPLKLEFLDQNGEIA-VVLNENSQLL-----VEPLRARREKVE 161
           + V+  G K   V++  +    + L+++G I   V+N  S LL     VE      E+ E
Sbjct: 40  VRVSDNGEKKSDVVVDVDEKNEKNLNESGVIEDCVMNGVSSLLKLKEDVEEEEEEEEEEE 99

Query: 162 DEDGDGANQLDDEEGTWSETFQSH 233
           +E+ DG ++ ++EE    E  + H
Sbjct: 100 EEEEDGEDEEEEEEEEEEEEEEEH 123


>At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein similar to flavanone 3-hydroxylase
           [Persea americana][GI:727410]; contains PF03171
           2OG-Fe(II) oxygenase superfamily domain
          Length = 341

 Score = 26.2 bits (55), Expect = 8.5
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = +2

Query: 254 HEAVSLDVVFPDADHVYGIPEHTDGLAL 337
           H AV+     P+ +  YG+P HTD  AL
Sbjct: 191 HMAVNYYPPCPEPELTYGLPAHTDPNAL 218


>At5g14120.1 68418.m01652 nodulin family protein similar to
           nodulin-like protein [Arabidopsis thaliana] GI:3329368,
           nodule-specific protein Nlj70 [Lotus japonicus]
           GI:3329366
          Length = 579

 Score = 26.2 bits (55), Expect = 8.5
 Identities = 11/33 (33%), Positives = 20/33 (60%)
 Frame = -1

Query: 212 PSSFLIIQLISPITVFVLYLFPSRP*RFHEQLR 114
           P+S +++  ++P  V V  +F  RP   H+Q+R
Sbjct: 174 PASLILMVAVTPAVVVVCLMFFIRPVGGHKQIR 206


>At1g20760.1 68414.m02600 calcium-binding EF hand family protein
           contains INTERPRO:IPR002048 calcium-binding EF-hand
           domain
          Length = 1019

 Score = 26.2 bits (55), Expect = 8.5
 Identities = 12/32 (37%), Positives = 18/32 (56%), Gaps = 2/32 (6%)
 Frame = +3

Query: 156 VEDEDG--DGANQLDDEEGTWSETFQSHHDSK 245
           V+D  G  D    ++D  G +SET   HH+S+
Sbjct: 732 VDDGSGPPDSPTHVEDNYGPFSETSDRHHESE 763


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,354,951
Number of Sequences: 28952
Number of extensions: 157203
Number of successful extensions: 743
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 711
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 736
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 605614832
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -