Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20515
(554 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC336.10c |tif512||translation initiation factor|Schizosacchar... 126 3e-30
SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizos... 126 3e-30
SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 27 1.9
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 2.5
SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces po... 27 2.5
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 26 4.3
SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosa... 25 5.7
SPAC1751.01c |gti1||gluconate transporter inducer Gti1|Schizosac... 25 7.5
SPBC16E9.17c |rem1||meiosis-specific cyclin Rem1|Schizosaccharom... 25 7.5
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 25 7.5
SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 25 7.5
SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosacchar... 25 9.9
SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|ch... 25 9.9
>SPBC336.10c |tif512||translation initiation
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 157
Score = 126 bits (303), Expect = 3e-30
Identities = 53/81 (65%), Positives = 70/81 (86%)
Frame = +1
Query: 256 STSKTGKHGHAKVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLM 435
STSKTGKHGHAKVH+V +DIFNG+KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM
Sbjct: 46 STSKTGKHGHAKVHIVALDIFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLM 104
Query: 436 ADNGDLREDLKIPDGDLGTQL 498
+G ++D+++P+G+LG ++
Sbjct: 105 TTDGTTKDDVRLPEGELGNEI 125
Score = 69.3 bits (162), Expect = 4e-13
Identities = 30/44 (68%), Positives = 36/44 (81%)
Frame = +2
Query: 122 MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVE 253
M + E FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+
Sbjct: 1 MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVD 44
>SPAC26H5.10c |tif51||translation initiation factor
eIF5A|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 126 bits (303), Expect = 3e-30
Identities = 53/81 (65%), Positives = 70/81 (86%)
Frame = +1
Query: 256 STSKTGKHGHAKVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLM 435
STSKTGKHGHAKVH+V +DIFNG+KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM
Sbjct: 46 STSKTGKHGHAKVHIVALDIFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLM 104
Query: 436 ADNGDLREDLKIPDGDLGTQL 498
+G ++D+++P+G+LG ++
Sbjct: 105 TTDGTTKDDVRLPEGELGNEI 125
Score = 69.3 bits (162), Expect = 4e-13
Identities = 30/44 (68%), Positives = 36/44 (81%)
Frame = +2
Query: 122 MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVE 253
M + E FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+
Sbjct: 1 MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVD 44
>SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 981
Score = 27.1 bits (57), Expect = 1.9
Identities = 15/49 (30%), Positives = 22/49 (44%)
Frame = +1
Query: 247 C*KSTSKTGKHGHAKVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDY 393
C T K A L+ D F+G ++ IC S N+ + V ED+
Sbjct: 869 CMTLTQILEKEADAYAPLLAYDKFDGDEFACICESMKNVKLDFVTLEDW 917
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 26.6 bits (56), Expect = 2.5
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +1
Query: 358 NMDVPHVKREDYQLTDISDDGYL 426
NM +P+++ E Q+ D + D YL
Sbjct: 968 NMSIPYIRGEYVQMIDANQDNYL 990
>SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 594
Score = 26.6 bits (56), Expect = 2.5
Identities = 15/43 (34%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +1
Query: 265 KTGKHGHAKVHLVGIDIFNGK-KYEDICPSTHNMDVPHVKRED 390
K G+H A +++ + N K +D CPST D+ H+ R D
Sbjct: 164 KIGQHLSAMGYVIPKEWTNTMVKLQDRCPSTSLKDIDHLFRVD 206
>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 713
Score = 25.8 bits (54), Expect = 4.3
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = -1
Query: 530 QQFLAAVESVRNCVPRSPSGILRSSRRSPLSA 435
+ +LAA+ V + +P SPS ++S +P A
Sbjct: 238 KHYLAAIRLVNSGIPDSPSEAIKSIHNNPRHA 269
>SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase
Ubp7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 875
Score = 25.4 bits (53), Expect = 5.7
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +1
Query: 376 VKREDYQLTDISDDGYL 426
+KREDY+LT ++ GYL
Sbjct: 91 IKREDYELTLCANCGYL 107
>SPAC1751.01c |gti1||gluconate transporter inducer
Gti1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 25.0 bits (52), Expect = 7.5
Identities = 14/44 (31%), Positives = 22/44 (50%)
Frame = +2
Query: 167 ASATFPMQCSALRKNGFVMLKGRPCKIVESPHPKPESTATLKFT 298
+SA+ PM+ S R + L G +I +P P + T+ FT
Sbjct: 666 SSASLPMRTSGNRFCHYSSLNGNHNRISARTNPYPINNQTMLFT 709
>SPBC16E9.17c |rem1||meiosis-specific cyclin
Rem1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 402
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -3
Query: 357 VCGGTDIFILFTIEDINPNQVNFSVAVLSG 268
+CG T +FI E+I+P V VL G
Sbjct: 219 LCGATALFIACKYEEIHPPTVKDLEIVLEG 248
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 25.0 bits (52), Expect = 7.5
Identities = 10/28 (35%), Positives = 18/28 (64%)
Frame = -1
Query: 512 VESVRNCVPRSPSGILRSSRRSPLSAIR 429
+ S R C P +PS ++ +++ S +SA R
Sbjct: 328 ISSTRLCSPENPSLLVNTAKTSKMSARR 355
>SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 814
Score = 25.0 bits (52), Expect = 7.5
Identities = 10/22 (45%), Positives = 13/22 (59%), Gaps = 4/22 (18%)
Frame = -2
Query: 259 WTFNNL----AWTTLQHNETVF 206
WT N+ WTTL+HN +F
Sbjct: 184 WTSENIDDTQKWTTLEHNGVIF 205
>SPAC57A7.04c |pabp||mRNA export shuttling protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 653
Score = 24.6 bits (51), Expect = 9.9
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +2
Query: 197 ALRKNGFVMLKGRPCKIVES 256
AL + + ++KGRPC+I+ S
Sbjct: 137 ALDELNYTLIKGRPCRIMWS 156
>SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 701
Score = 24.6 bits (51), Expect = 9.9
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -1
Query: 314 ISIPTK*TLAWPCFPVLDVDFQQSC 240
++IPTK T W C P+ + + SC
Sbjct: 4 LNIPTKNTFHWSCDPLFNELKRLSC 28
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,405,483
Number of Sequences: 5004
Number of extensions: 51260
Number of successful extensions: 166
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 231978230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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