Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20509
(692 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32940.1 68415.m04038 PAZ domain-containing protein / piwi do... 37 0.011
At1g69440.1 68414.m07979 PAZ domain-containing protein / piwi do... 35 0.059
At2g27880.1 68415.m03380 argonaute protein, putative / AGO, puta... 34 0.10
At1g28640.1 68414.m03527 GDSL-motif lipase, putative strong simi... 31 0.55
At5g63570.1 68418.m07979 glutamate-1-semialdehyde 2,1-aminomutas... 30 1.7
At5g15070.1 68418.m01766 expressed protein 30 1.7
At1g04445.1 68414.m00436 zinc finger (C2H2 type) family protein ... 30 1.7
At3g48730.1 68416.m05321 glutamate-1-semialdehyde 2,1-aminomutas... 29 2.2
At5g35970.1 68418.m04332 DNA-binding protein, putative similar t... 29 3.9
At1g03530.1 68414.m00334 expressed protein similar to hypothetic... 29 3.9
At3g01310.1 68416.m00042 expressed protein similar to unknown pr... 28 5.1
At4g15200.1 68417.m02329 formin homology 2 domain-containing pro... 27 8.9
At4g09290.1 68417.m01537 hypothetical protein 27 8.9
At2g13790.1 68415.m01522 leucine-rich repeat family protein / pr... 27 8.9
>At2g32940.1 68415.m04038 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|O04379 Argonaute
protein (AGO1) {Arabidopsis thaliana}, SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 878
Score = 37.1 bits (82), Expect = 0.011
Identities = 25/64 (39%), Positives = 32/64 (50%), Gaps = 5/64 (7%)
Frame = +3
Query: 327 GTSGTPLDLLANYFTVET-TPKWGLYQYHVDISPEE----DSTGVRKALMRVHSKTLGGY 491
GT+G P++L N+F V P YQY V I+ E D TG+ + LM KT
Sbjct: 33 GTTGNPIELCTNHFNVSVRQPDVVFYQYTVSITTENGDAVDGTGISRKLMDQLFKTYSSD 92
Query: 492 LFDG 503
L DG
Sbjct: 93 L-DG 95
>At1g69440.1 68414.m07979 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 990
Score = 34.7 bits (76), Expect = 0.059
Identities = 24/87 (27%), Positives = 43/87 (49%), Gaps = 5/87 (5%)
Frame = +3
Query: 258 GGRVLPETISILRTRPEAVTSKKGTSGTPLDLLANYFTVETTPKWGLYQYHVDISPE--- 428
G + E ++ RP+ G G+ + LLAN+F V+ +Y Y+V+ISP+
Sbjct: 139 GSIAIEEAALVVAKRPDF----GGQDGSVIYLLANHFLVKFDSSQRIYHYNVEISPQPSK 194
Query: 429 EDSTGVRKALMRVHSKTLGGYL--FDG 503
E + +++ L+ + G + FDG
Sbjct: 195 EIARMIKQKLVETDRNSFSGVVPAFDG 221
>At2g27880.1 68415.m03380 argonaute protein, putative / AGO,
putative similar to SP|O04379 Argonaute protein (AGO1)
{Arabidopsis thaliana}; contains Pfam profiles PF02170:
PAZ domain, PF02171: Piwi domain
Length = 997
Score = 33.9 bits (74), Expect = 0.10
Identities = 23/69 (33%), Positives = 36/69 (52%), Gaps = 4/69 (5%)
Frame = +3
Query: 273 PETISILRTRPEAVTSK----KGTSGTPLDLLANYFTVETTPKWGLYQYHVDISPEEDST 440
PE S+ +AVT +GT G + + AN+F V+ + LY Y V I+PE S
Sbjct: 132 PEMTSLPPASSKAVTFPVRPGRGTLGKKVMVRANHFLVQVADR-DLYHYDVSINPEVISK 190
Query: 441 GVRKALMRV 467
V + +M++
Sbjct: 191 TVNRNVMKL 199
>At1g28640.1 68414.m03527 GDSL-motif lipase, putative strong
similarity to lipase GB:AAA93262 GI:1145627 [Arabidopsis
thaliana]
Length = 390
Score = 31.5 bits (68), Expect = 0.55
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = +2
Query: 158 ISMCNNACKN*NWDPIHLEVVMHHP*LAVALDGGWKSPS 274
+S C N + NWD HL H V L+G + SP+
Sbjct: 333 VSCCQNPSEYVNWDGYHLTEATHQKMAQVILNGTYASPA 371
>At5g63570.1 68418.m07979 glutamate-1-semialdehyde 2,1-aminomutase 1
(GSA 1) / glutamate-1-semialdehyde aminotransferase 1
(GSA-AT 1) identical to GSA 1 [SP|P42799]
Length = 474
Score = 29.9 bits (64), Expect = 1.7
Identities = 23/81 (28%), Positives = 39/81 (48%), Gaps = 1/81 (1%)
Frame = +3
Query: 255 GGGRVLPETISILRTRPEAVTSKKGT-SGTPLDLLANYFTVETTPKWGLYQYHVDISPEE 431
GG R + E ++ P + GT SG PL + A T++ + G Y+Y +D +E
Sbjct: 326 GGRRDIMEMVA-----PAGPMYQAGTLSGNPLAMTAGIHTLKRLKQAGTYEY-LDKITKE 379
Query: 432 DSTGVRKALMRVHSKTLGGYL 494
+ G+ +A + GGY+
Sbjct: 380 LTNGILEAGKKTGHPMCGGYI 400
>At5g15070.1 68418.m01766 expressed protein
Length = 1049
Score = 29.9 bits (64), Expect = 1.7
Identities = 19/52 (36%), Positives = 28/52 (53%)
Frame = -2
Query: 328 PFLDVTASGRVRKMEIVSGRTLPPPVESHGQLRMMHHHLQMNGVPVLIFACV 173
P V A +RK +V+ L P H + R ++ HL+M G+PV +ACV
Sbjct: 75 PLEKVQAYSSLRKPFLVN--ELDPQYLLHDR-RKVYEHLEMYGIPVPRYACV 123
>At1g04445.1 68414.m00436 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 172
Score = 29.9 bits (64), Expect = 1.7
Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
Frame = +3
Query: 237 WPWLSTGGGRVLPETISILRTRPE--AVTSKKGTSGTPLDL 353
+P S+GG R LP + + RPE AV G + +P+DL
Sbjct: 128 FPETSSGGTRTLPLLCQLDQWRPEDSAVAENGGANSSPIDL 168
>At3g48730.1 68416.m05321 glutamate-1-semialdehyde 2,1-aminomutase 2
(GSA 2) / glutamate-1-semialdehyde aminotransferase 2
(GSA-AT 2) identical to GSA2 [SP|Q42522]
Length = 472
Score = 29.5 bits (63), Expect = 2.2
Identities = 23/81 (28%), Positives = 39/81 (48%), Gaps = 1/81 (1%)
Frame = +3
Query: 255 GGGRVLPETISILRTRPEAVTSKKGT-SGTPLDLLANYFTVETTPKWGLYQYHVDISPEE 431
GG R + E ++ P + GT SG PL + A T++ + G Y+Y +D +E
Sbjct: 324 GGRRDIMEMVA-----PAGPMYQAGTLSGNPLAMTAGIHTLKRLSQPGTYEY-LDKITKE 377
Query: 432 DSTGVRKALMRVHSKTLGGYL 494
+ G+ +A + GGY+
Sbjct: 378 LTNGILEAGKKTGHAMCGGYI 398
>At5g35970.1 68418.m04332 DNA-binding protein, putative similar to
SWISS-PROT:Q60560 DNA-binding protein SMUBP-2
(Immunoglobulin MU binding protein 2, SMUBP-2)
[Mesocricetus auratus]
Length = 961
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/50 (28%), Positives = 19/50 (38%), Gaps = 1/50 (2%)
Frame = -1
Query: 215 PPDEWGPSSNFCMRCCTSISPGC-SCVVGVTRCEARPACAVGVGTPALTG 69
PP P C+R C S G +C G C++GV + G
Sbjct: 352 PPTTLSPGDMVCIRVCDSRGAGATACTQGFVHNLGEDGCSIGVALESRHG 401
>At1g03530.1 68414.m00334 expressed protein similar to hypothetical
protein GB:O14360
Length = 797
Score = 28.7 bits (61), Expect = 3.9
Identities = 15/41 (36%), Positives = 18/41 (43%), Gaps = 1/41 (2%)
Frame = -1
Query: 305 WTCAQDGNSFRKDSSTPRRE-PRPTTDDASPPPDEWGPSSN 186
WT Q +S + S P+ PRP D P W P SN
Sbjct: 539 WTKNQGSSSLSSNRSDPQMGGPRPQMDGFPPNNAAWRPQSN 579
>At3g01310.1 68416.m00042 expressed protein similar to unknown
protein GB:BAA24863 [Homo sapiens], unknown protein
GB:BAA20831 [Homo sapiens], unknown protein GB:AAB42264
[Caenorhabditis elegans]
Length = 1056
Score = 28.3 bits (60), Expect = 5.1
Identities = 18/52 (34%), Positives = 27/52 (51%)
Frame = -2
Query: 328 PFLDVTASGRVRKMEIVSGRTLPPPVESHGQLRMMHHHLQMNGVPVLIFACV 173
P A +RK +V+ L P H + R ++ HL+M G+PV +ACV
Sbjct: 82 PLEKAQAYAALRKPFLVN--ELDPQYLLHDR-RKVYEHLEMYGIPVPRYACV 130
>At4g15200.1 68417.m02329 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 600
Score = 27.5 bits (58), Expect = 8.9
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -1
Query: 266 SSTPRREPRPTTDDASPPPDEWGPS 192
+S+P P T D++SP P E PS
Sbjct: 102 ASSPNEPPAETPDESSPSPSEETPS 126
>At4g09290.1 68417.m01537 hypothetical protein
Length = 376
Score = 27.5 bits (58), Expect = 8.9
Identities = 17/61 (27%), Positives = 29/61 (47%), Gaps = 6/61 (9%)
Frame = -1
Query: 368 KIVGKQIQGSTRSTFLRCY----SFWTCAQDGNSFRKDSSTPRREPRPTTDDA--SPPPD 207
+++G Q++ + F R F T Q GN + TP+ +PT + + SPPP
Sbjct: 143 EVIGDQVEARIDAKFERRVVIREMFLTKEQQGNPKPQTQQTPKGPRKPTNNQSVTSPPPS 202
Query: 206 E 204
+
Sbjct: 203 K 203
>At2g13790.1 68415.m01522 leucine-rich repeat family protein /
protein kinase family protein
Length = 620
Score = 27.5 bits (58), Expect = 8.9
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -1
Query: 281 SFRKDSSTPRREPRPTTDDASPPPDEWG 198
SF +S T EP PT+ +PPP G
Sbjct: 199 SFANNSLTDLPEPPPTSTSPTPPPPSGG 226
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,812,825
Number of Sequences: 28952
Number of extensions: 363509
Number of successful extensions: 1483
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1482
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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