Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20508
         (698 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    71   1e-13
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    49   6e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    42   1e-04
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    37   0.003
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    36   0.004
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    33   0.030
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    27   2.0  
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos...    27   3.4  
SPAC6F6.11c |||pyridoxine-pyridoxal-pyridoxamine kinase |Schizos...    26   6.0  
SPCC1494.06c |||ATP-dependent RNA helicase Dbp9 |Schizosaccharom...    25   7.9  
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c...    25   7.9  
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual      25   7.9  
SPAC23H4.14 |vam6|vps39|guanyl-nucleotide exchange factor Vma6|S...    25   7.9  

>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 71.3 bits (167), Expect = 1e-13
 Identities = 29/63 (46%), Positives = 46/63 (73%)
 Frame = +2

Query: 278 VALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKYMRAQVGPSS 457
           ++L +VDCTE G   C ++S+ GYPTL +F+ G+  S+Y+GPR+ + +VKYMR Q+ P+ 
Sbjct: 73  ISLVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTV 131

Query: 458 KEL 466
           K +
Sbjct: 132 KPI 134



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 21/51 (41%), Positives = 29/51 (56%)
 Frame = +3

Query: 99  YLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAA 251
           + C +AE  V  +     + +++     +V FYAPWCGHCK L PEY  AA
Sbjct: 17  FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAA 65



 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 20/43 (46%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
 Frame = +3

Query: 114 AEEDVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 239
           ++ED++ L   +F   V+ +    LV FYAPWCGHCK L P Y
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTY 395



 Score = 32.7 bits (71), Expect = 0.052
 Identities = 22/71 (30%), Positives = 34/71 (47%), Gaps = 4/71 (5%)
 Frame = +2

Query: 263 TDDPPVALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSS--EYNGPRESNGIVKYM- 433
           +DD  V +AK+D TE   S     S+SG+PT+  F+  +  +   Y G R    +  ++ 
Sbjct: 403 SDDSNVVVAKIDATENDISV----SISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFID 458

Query: 434 -RAQVGPSSKE 463
             A   P  KE
Sbjct: 459 KHASFEPIKKE 469


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 49.2 bits (112), Expect = 6e-07
 Identities = 22/68 (32%), Positives = 36/68 (52%)
 Frame = +3

Query: 36  KAPAKFEMFGSLKFVLLLGIIYLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGH 215
           + P  F +F +  F L+ G+         + ++L   +F   +     +LV+FYAPWCG+
Sbjct: 4   RIPTLFTLFLAC-FSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGY 62

Query: 216 CKRLKPEY 239
           CK+L P Y
Sbjct: 63  CKKLVPTY 70



 Score = 39.5 bits (88), Expect = 5e-04
 Identities = 22/66 (33%), Positives = 37/66 (56%), Gaps = 6/66 (9%)
 Frame = +2

Query: 293 VDC-TEGGKSTCEQFSVSGYPTLKIF---RKGE--LSSEYNGPRESNGIVKYMRAQVGPS 454
           VDC  +  ++ C Q+ V G+PT+K+     KG    S++YNG R    + K++   + PS
Sbjct: 86  VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSI-PS 144

Query: 455 SKELLT 472
             ++LT
Sbjct: 145 KVKILT 150


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 18/40 (45%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
 Frame = +3

Query: 123 DVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 239
           +V++L   +F   V+      LV FYA WCG+CKRL P Y
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180



 Score = 39.1 bits (87), Expect = 6e-04
 Identities = 14/20 (70%), Positives = 15/20 (75%)
 Frame = +3

Query: 180 ALVMFYAPWCGHCKRLKPEY 239
           AL+ FYA WCGHCK L P Y
Sbjct: 42  ALIEFYATWCGHCKSLAPVY 61


>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 36.7 bits (81), Expect = 0.003
 Identities = 12/32 (37%), Positives = 21/32 (65%)
 Frame = +3

Query: 144 SDFSAVLSQHDTALVMFYAPWCGHCKRLKPEY 239
           S+F +++ Q    +V F+A WCG CK + P++
Sbjct: 9   SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF 40


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 36.3 bits (80), Expect = 0.004
 Identities = 14/29 (48%), Positives = 18/29 (62%)
 Frame = +3

Query: 147 DFSAVLSQHDTALVMFYAPWCGHCKRLKP 233
           D++  +S     +V FYA WCG CK LKP
Sbjct: 27  DYNTRISADKVTVVDFYADWCGPCKYLKP 55


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 33.5 bits (73), Expect = 0.030
 Identities = 15/33 (45%), Positives = 21/33 (63%)
 Frame = +3

Query: 135 LTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKP 233
           LTD+D  + +S+  T  + +Y P CG CKRL P
Sbjct: 31  LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGP 62



 Score = 28.3 bits (60), Expect = 1.1
 Identities = 15/49 (30%), Positives = 25/49 (51%)
 Frame = +2

Query: 284 LAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKY 430
           +A ++C    K  C+Q+S+  +PT  +F K E   EY G      +V +
Sbjct: 333 VAHINCAVS-KRACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSF 379


>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 9/19 (47%), Positives = 12/19 (63%)
 Frame = +3

Query: 195 YAPWCGHCKRLKPEYAVAA 251
           YA WCG CK + P ++  A
Sbjct: 27  YADWCGPCKAISPLFSQLA 45


>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 640

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = +1

Query: 127 FSILQIPTFRLFYLNMIQPWSCF 195
           F  LQ+  FR  + N+++PW CF
Sbjct: 208 FYCLQLQMFRKMH-NIVRPWDCF 229


>SPAC6F6.11c |||pyridoxine-pyridoxal-pyridoxamine kinase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 309

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 11/21 (52%), Positives = 16/21 (76%)
 Frame = +3

Query: 570 DKLREEVTFAHSSANEVLEKT 632
           DKL++ V  A SS +EV++KT
Sbjct: 248 DKLKKSVEMALSSVHEVIQKT 268


>SPCC1494.06c |||ATP-dependent RNA helicase Dbp9
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 595

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 19/59 (32%), Positives = 28/59 (47%)
 Frame = -3

Query: 420 MPLDSLGPLYSEESSPFLKIFSVGYPDTENCSQVLLPPSVQSTLASATGGSSVFNSRRL 244
           +PLD L  L  +E+   L   S GY +        LP   QS L SAT   ++ + ++L
Sbjct: 160 LPLDKLKFLVIDEADLML---SFGYNEDMKTLSRSLPRGTQSFLMSATLSKNIASLQKL 215


>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 8/15 (53%), Positives = 11/15 (73%)
 Frame = +3

Query: 183 LVMFYAPWCGHCKRL 227
           L+ FYAPW   CK++
Sbjct: 24  LLNFYAPWAAPCKQM 38


>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1489

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 10/31 (32%), Positives = 18/31 (58%)
 Frame = -3

Query: 447  PTWARMYLTMPLDSLGPLYSEESSPFLKIFS 355
            P   ++Y+T+  D +G    E +SP +K+ S
Sbjct: 1414 PQKEKLYITISADEVGKFILEATSPTVKVSS 1444


>SPAC23H4.14 |vam6|vps39|guanyl-nucleotide exchange factor
           Vma6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 905

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/51 (25%), Positives = 22/51 (43%)
 Frame = +3

Query: 543 PEREFLKTADKLREEVTFAHSSANEVLEKTGYKNNVVLYRPKRLQNKFEDS 695
           P+   +   D+  E  T +     + LE   YK +++      L NKF D+
Sbjct: 606 PQNSIVIFIDENSEASTISKGVVLKYLETISYKVSIIYLEKLLLDNKFNDT 656


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,845,965
Number of Sequences: 5004
Number of extensions: 57119
Number of successful extensions: 181
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)