Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20507
(645 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC328.10c |rps502|rps5-2|40S ribosomal protein S5|Schizosaccha... 136 3e-33
SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces p... 136 3e-33
SPAC16E8.10c |||mitochondrial ribosomal protein subunit S7|Schiz... 33 0.047
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.57
SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr ... 27 1.8
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe... 27 2.3
SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces... 27 3.1
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 27 3.1
SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual 25 7.1
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 25 9.3
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 25 9.3
>SPAC328.10c |rps502|rps5-2|40S ribosomal protein
S5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 203
Score = 136 bits (328), Expect = 3e-33
Identities = 67/89 (75%), Positives = 76/89 (85%)
Frame = +1
Query: 244 QKTEAVRIVKHAFEIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVDVSPL 423
+K A RIVKHAFEII LLT +NPLQVLV A+ GPREDSTRIG AGTVRRQAVDVSPL
Sbjct: 84 KKLLATRIVKHAFEIIALLTDQNPLQVLVDAVAACGPREDSTRIGSAGTVRRQAVDVSPL 143
Query: 424 RRVNQAIWLLCTGAREAAFRNIKTIAECV 510
RRVNQA+ L+ GAREAAFRN+K+I+EC+
Sbjct: 144 RRVNQALALITIGAREAAFRNVKSISECL 172
Score = 88.2 bits (209), Expect = 9e-19
Identities = 44/71 (61%), Positives = 55/71 (77%)
Frame = +2
Query: 41 IKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYAHKRFRKAQCPIVERLTNSL 220
IKLF ++ V+V D+SL DYI++ + LPH+AGR+ KRFRKA+C IVERLTNSL
Sbjct: 18 IKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQTKRFRKARCFIVERLTNSL 75
Query: 221 MMHGRNNGKKL 253
MM+GRNNGKKL
Sbjct: 76 MMNGRNNGKKL 86
Score = 61.7 bits (143), Expect = 9e-11
Identities = 29/31 (93%), Positives = 31/31 (100%)
Frame = +3
Query: 510 ADELINAAKGSSNSYAIKKKDELERVAKSNR 602
A+E+INAAKGSSNSYAIKKKDELERVAKSNR
Sbjct: 173 AEEIINAAKGSSNSYAIKKKDELERVAKSNR 203
>SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 203
Score = 136 bits (328), Expect = 3e-33
Identities = 67/89 (75%), Positives = 76/89 (85%)
Frame = +1
Query: 244 QKTEAVRIVKHAFEIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVDVSPL 423
+K A RIVKHAFEII LLT +NPLQVLV A+ GPREDSTRIG AGTVRRQAVDVSPL
Sbjct: 84 KKLLATRIVKHAFEIIALLTDQNPLQVLVDAVAACGPREDSTRIGSAGTVRRQAVDVSPL 143
Query: 424 RRVNQAIWLLCTGAREAAFRNIKTIAECV 510
RRVNQA+ L+ GAREAAFRN+K+I+EC+
Sbjct: 144 RRVNQALALITIGAREAAFRNVKSISECL 172
Score = 88.6 bits (210), Expect = 7e-19
Identities = 48/86 (55%), Positives = 60/86 (69%), Gaps = 3/86 (3%)
Frame = +2
Query: 5 TMSLPQAADIPE---IKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYAHKRF 175
T SL + E IKLF ++ V+V D+SL DYI++ + LPH+AGR+ KRF
Sbjct: 3 TSSLTPGVSLDENGSIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQTKRF 60
Query: 176 RKAQCPIVERLTNSLMMHGRNNGKKL 253
RKA+C IVERLTNSLMM+GRNNGKKL
Sbjct: 61 RKARCFIVERLTNSLMMNGRNNGKKL 86
Score = 61.7 bits (143), Expect = 9e-11
Identities = 29/31 (93%), Positives = 31/31 (100%)
Frame = +3
Query: 510 ADELINAAKGSSNSYAIKKKDELERVAKSNR 602
A+E+INAAKGSSNSYAIKKKDELERVAKSNR
Sbjct: 173 AEEIINAAKGSSNSYAIKKKDELERVAKSNR 203
>SPAC16E8.10c |||mitochondrial ribosomal protein subunit
S7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 259
Score = 32.7 bits (71), Expect = 0.047
Identities = 17/37 (45%), Positives = 21/37 (56%)
Frame = +1
Query: 244 QKTEAVRIVKHAFEIIHLLTGENPLQVLVTAIINSGP 354
+K +A +IV A II TGENP+ VL AI P
Sbjct: 138 KKAKAEKIVATALSIIQKETGENPIDVLKQAIAEISP 174
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 29.1 bits (62), Expect = 0.57
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = +3
Query: 255 GRTYCQTCV*NYSLVNWRKPSASTRDCHYQLWT 353
G TYC C L+NW K S S C +L+T
Sbjct: 101 GHTYCYEC-----LLNWLKESKSCPTCRQKLYT 128
>SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 335
Score = 27.5 bits (58), Expect = 1.8
Identities = 17/51 (33%), Positives = 28/51 (54%)
Frame = +1
Query: 277 AFEIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVDVSPLRR 429
+ +I HL+TG N + T ++N R D R+ R+ + Q+ D SP +R
Sbjct: 159 SLDIHHLVTGHNADDIAETILMNL-LRGDVARLPRSTEITTQS-DSSPTKR 207
>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 27.1 bits (57), Expect = 2.3
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +2
Query: 20 QAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYA 163
+ ADI + FGR ++++++ + I+V+EKYAK P R A
Sbjct: 7 KVADI-SLAAFGR---KELEIAENEMPGLIAVREKYAKSQPLKGARIA 50
>SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 567
Score = 26.6 bits (56), Expect = 3.1
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +1
Query: 529 QLRVHLTPTPSKRRTSWSVLLNPTV 603
Q ++H P P +RR S + L NP++
Sbjct: 124 QQKIHRNPQPRRRRRSTTALPNPSL 148
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 26.6 bits (56), Expect = 3.1
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +2
Query: 347 LDPVKIRLGSVVRVQFVVKPLMFHPCAESTKQSGFCAQV 463
++PV R SVV V +P+ +H +S ++G Q+
Sbjct: 273 IEPVSSRQSSVVNNNSVQQPVAYHAFVQSPTENGTLPQL 311
>SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual
Length = 613
Score = 25.4 bits (53), Expect = 7.1
Identities = 16/54 (29%), Positives = 28/54 (51%)
Frame = -2
Query: 182 LYGNACVHTCQLNEVNILRTSL*RKCSPAETYQRLAHRSNSICRKA*FQECRRL 21
L N C+ LNE ++ + SL R P++ ++ R +S+ +K+ E R L
Sbjct: 147 LLNNCCIGIANLNEDSVHKISLTRSGKPSQLV-TVSCRHSSVIQKSYGSEKRYL 199
>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 25.0 bits (52), Expect = 9.3
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +3
Query: 9 CLYHKPPTFLKSSFS 53
C H P TFL SSFS
Sbjct: 117 CFIHPPITFLSSSFS 131
>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 750
Score = 25.0 bits (52), Expect = 9.3
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +3
Query: 516 ELINAAKGSSNSYAIKKKDELER 584
E +N+ + NS ++KKDELE+
Sbjct: 133 EKLNSEISNQNSLILQKKDELEK 155
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,764,984
Number of Sequences: 5004
Number of extensions: 55696
Number of successful extensions: 149
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 289756512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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