BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20505 (680 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g34355.1 68415.m04205 nodulin-related contains 14 transmembra... 31 0.71 At4g25570.1 68417.m03685 cytochrome B561 family protein contains... 29 2.8 At5g64270.1 68418.m08074 splicing factor, putative similar to sp... 29 3.8 At1g60140.1 68414.m06775 glycosyl transferase family 20 protein ... 28 5.0 At4g10970.3 68417.m01785 expressed protein 27 8.7 At4g10970.2 68417.m01784 expressed protein 27 8.7 At4g10970.1 68417.m01783 expressed protein 27 8.7 At3g21970.1 68416.m02772 receptor-like protein kinase-related co... 27 8.7 At1g76290.1 68414.m08860 AMP-dependent synthetase and ligase fam... 27 8.7 >At2g34355.1 68415.m04205 nodulin-related contains 14 transmembrane domains; supported by tandem duplication of nodulin -related protein (TIGR_Ath1:At2g34350) [Arabidopsis thaliana] Length = 523 Score = 31.1 bits (67), Expect = 0.71 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = -3 Query: 129 LLSLDVGGLWSLSPSRTSRLGGVRSFFVSLLTVSLVG 19 L+ + G WSL P+ TS + G+R T+S+ G Sbjct: 415 LIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAG 451 >At4g25570.1 68417.m03685 cytochrome B561 family protein contains Pfam domain, PF03188: Cytochrome b561 Length = 239 Score = 29.1 bits (62), Expect = 2.8 Identities = 14/66 (21%), Positives = 32/66 (48%) Frame = +1 Query: 295 LLYQIGETQGQYGVGFIIKQHLKQYVEEFIGISERIAIMNLNLPGYKKPWSIVQIYSPTE 474 +L+ I G +G+ K H + ++ + I I ++L G++ +S + + P Sbjct: 85 ILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIGVISLYGFQWVYSFIVFFFPGG 144 Query: 475 QSNLET 492 +NL++ Sbjct: 145 STNLKS 150 >At5g64270.1 68418.m08074 splicing factor, putative similar to splicing factor 3B subunit 1 (Spliceosome associated protein 155) (SAP 155) (SF3b155) (Pre-mRNA splicing factor SF3b 155 kDa subunit) (146 kDa nuclear protein) SP:O57683 from [Xenopus laevis] Length = 1269 Score = 28.7 bits (61), Expect = 3.8 Identities = 18/71 (25%), Positives = 32/71 (45%) Frame = +1 Query: 175 LGKRRKSYGTDICAKIYQMGHNWFERRRRMGESITCHPDYLLYQIGETQGQYGVGFIIKQ 354 LG+R K Y IC I +N + R+ + ++ Q GE Q +G ++ + Sbjct: 886 LGQRVKPYLPQICGTIKWRLNNKSAKVRQQAADLISRIAVVMKQCGEEQLMGHLGVVLYE 945 Query: 355 HLKQYVEEFIG 387 +L + E +G Sbjct: 946 YLGEEYPEVLG 956 >At1g60140.1 68414.m06775 glycosyl transferase family 20 protein / trehalose-phosphatase family protein contains Pfam profile: PF02358 trehalose-phosphatase Length = 861 Score = 28.3 bits (60), Expect = 5.0 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +2 Query: 218 KSIKWDIIGLSEGEEWAKVLLVIQTIY 298 KS W+ GLS+ EW KV+ I +Y Sbjct: 666 KSSDWETSGLSDDLEWKKVVEPIMRLY 692 >At4g10970.3 68417.m01785 expressed protein Length = 217 Score = 27.5 bits (58), Expect = 8.7 Identities = 20/78 (25%), Positives = 38/78 (48%), Gaps = 3/78 (3%) Frame = +2 Query: 428 DIKNRGLLYKYIHLQSNQIWKR*ITSTYFKRSHSRAYAQKPYSHGRLQWPNWSK---TPR 598 D++ K + Q NQ +T+T +++ S +PY+ GR+ N S+ P Sbjct: 71 DVRQGAFAKKRSNFQGNQFP---VTTTVARKAASATPRGRPYNGGRMTNTNQSRFIAPPA 127 Query: 599 RKRNTRTIFI**QDQKQK 652 + R ++ F+ Q Q+Q+ Sbjct: 128 QNRASQRGFVGKQQQQQR 145 >At4g10970.2 68417.m01784 expressed protein Length = 217 Score = 27.5 bits (58), Expect = 8.7 Identities = 20/78 (25%), Positives = 38/78 (48%), Gaps = 3/78 (3%) Frame = +2 Query: 428 DIKNRGLLYKYIHLQSNQIWKR*ITSTYFKRSHSRAYAQKPYSHGRLQWPNWSK---TPR 598 D++ K + Q NQ +T+T +++ S +PY+ GR+ N S+ P Sbjct: 71 DVRQGAFAKKRSNFQGNQFP---VTTTVARKAASATPRGRPYNGGRMTNTNQSRFIAPPA 127 Query: 599 RKRNTRTIFI**QDQKQK 652 + R ++ F+ Q Q+Q+ Sbjct: 128 QNRASQRGFVGKQQQQQR 145 >At4g10970.1 68417.m01783 expressed protein Length = 217 Score = 27.5 bits (58), Expect = 8.7 Identities = 20/78 (25%), Positives = 38/78 (48%), Gaps = 3/78 (3%) Frame = +2 Query: 428 DIKNRGLLYKYIHLQSNQIWKR*ITSTYFKRSHSRAYAQKPYSHGRLQWPNWSK---TPR 598 D++ K + Q NQ +T+T +++ S +PY+ GR+ N S+ P Sbjct: 71 DVRQGAFAKKRSNFQGNQFP---VTTTVARKAASATPRGRPYNGGRMTNTNQSRFIAPPA 127 Query: 599 RKRNTRTIFI**QDQKQK 652 + R ++ F+ Q Q+Q+ Sbjct: 128 QNRASQRGFVGKQQQQQR 145 >At3g21970.1 68416.m02772 receptor-like protein kinase-related contains Pfam profile: PF01657 Domain of unknown function that is usually associated with protein kinase domain Pfam:PF00069; similar to receptor-like protein kinase 4 (GI:13506745) and receptor-like protein kinase 5 (GI:13506747) [Arabidopsis thaliana] Length = 264 Score = 27.5 bits (58), Expect = 8.7 Identities = 15/50 (30%), Positives = 24/50 (48%), Gaps = 1/50 (2%) Frame = +1 Query: 289 DYLLYQIGETQGQYGVGFIIKQHLKQYVEEFIGISERIAIMNL-NLPGYK 435 DYL +Q TQG+Y G +++L Q + + R + N+ YK Sbjct: 33 DYLNHQCNNTQGRYTHGSTFEKNLNQVLHNISNLDLRYGYAYVSNVVAYK 82 >At1g76290.1 68414.m08860 AMP-dependent synthetase and ligase family protein similar to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501 Length = 546 Score = 27.5 bits (58), Expect = 8.7 Identities = 18/55 (32%), Positives = 26/55 (47%) Frame = +1 Query: 376 EFIGISERIAIMNLNLPGYKKPWSIVQIYSPTEQSNLETINNFYLF*TKPFKSIR 540 EF+ ++E + N+ +KP I +PTEQS E +F FK IR Sbjct: 120 EFLSVAEESLSLLSNIE--EKPLIITITENPTEQSKYEQYEDFLSTGNPNFKPIR 172 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,294,362 Number of Sequences: 28952 Number of extensions: 333528 Number of successful extensions: 816 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 794 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 816 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1438152744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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