Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20477
(404 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Su... 28 0.48
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 27 0.84
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 27 1.1
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 27 1.5
SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces... 27 1.5
SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual 25 3.4
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc... 25 4.5
SPBC16C6.03c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 5.9
SPBC409.09c |mis13|cnl1|kinetochore protein Mis13|Schizosaccharo... 25 5.9
SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 25 5.9
SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual 24 7.8
SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding... 24 7.8
SPAC1786.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 24 7.8
>SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase
Sum3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 636
Score = 28.3 bits (60), Expect = 0.48
Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = +1
Query: 127 NKKTRIGGGAGRK--FDANKRTGRGGGAGFRSNRSGGVQR 240
N+ GGG GR+ + + G G G+R+NR G R
Sbjct: 78 NRGGHYGGGEGRQNNYRGGREGGYSNGGGYRNNRGFGQWR 117
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 27.5 bits (58), Expect = 0.84
Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 5/61 (8%)
Frame = +1
Query: 88 DQIEMALDDIIKANK---KTRIGGGAGRKFDANKRTGRGGGAGFRSNRSG--GVQRGRNR 252
D++ ++ D +I ++ K + G K N GRGG GFR R G G G +R
Sbjct: 100 DKVYLSGDKLIPLDRFLPKPKTVGPKKPKGARNGPAGRGGRGGFRGGRGGSRGGFGGNSR 159
Query: 253 G 255
G
Sbjct: 160 G 160
Score = 26.2 bits (55), Expect = 1.9
Identities = 17/39 (43%), Positives = 20/39 (51%)
Frame = +1
Query: 148 GGAGRKFDANKRTGRGGGAGFRSNRSGGVQRGRNRGA*R 264
GG+ F N R G GGG+ R GG RG +RG R
Sbjct: 148 GGSRGGFGGNSRGGFGGGS--RGGFGGG-SRGGSRGGFR 183
Score = 25.8 bits (54), Expect = 2.6
Identities = 16/37 (43%), Positives = 16/37 (43%), Gaps = 1/37 (2%)
Frame = +1
Query: 148 GGAGRKFDANKRTGRGGG-AGFRSNRSGGVQRGRNRG 255
G AGR R GRGG GF N GG G G
Sbjct: 133 GPAGRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGG 169
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 27.1 bits (57), Expect = 1.1
Identities = 16/35 (45%), Positives = 17/35 (48%)
Frame = +1
Query: 151 GAGRKFDANKRTGRGGGAGFRSNRSGGVQRGRNRG 255
G GR R GRGG G R SGG RG +G
Sbjct: 37 GGGRGGARGGRGGRGGARGGRGGSSGG--RGGAKG 69
Score = 26.2 bits (55), Expect = 1.9
Identities = 13/33 (39%), Positives = 15/33 (45%)
Frame = +1
Query: 139 RIGGGAGRKFDANKRTGRGGGAGFRSNRSGGVQ 237
R G GR R GRGG +G R GG +
Sbjct: 40 RGGARGGRGGRGGARGGRGGSSGGRGGAKGGAK 72
Score = 24.2 bits (50), Expect = 7.8
Identities = 15/33 (45%), Positives = 15/33 (45%)
Frame = +1
Query: 148 GGAGRKFDANKRTGRGGGAGFRSNRSGGVQRGR 246
GGA R GRGG G R R GG GR
Sbjct: 33 GGARGGGRGGARGGRGGRGGARGGR-GGSSGGR 64
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 26.6 bits (56), Expect = 1.5
Identities = 21/60 (35%), Positives = 25/60 (41%)
Frame = +1
Query: 139 RIGGGAGRKFDANKRTGRGGGAGFRSNRSGGVQRGRNRGA*RNLPTIRG*FQ*LDDSKIT 318
R GGG+ R G GG GF G RGR RG R+ RG +K+T
Sbjct: 444 RTGGGS-----RGGRGGFGGRGGFGGRGGFGGGRGRGRGGARSGNPNRGSVAPFSGNKVT 498
>SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 609
Score = 26.6 bits (56), Expect = 1.5
Identities = 20/72 (27%), Positives = 33/72 (45%), Gaps = 3/72 (4%)
Frame = -2
Query: 313 F*NH-LIIGITLE*LVDFVMPHDFYLVVHHQSGCYEIQHRLHVLFF--CLHQIFFQHHLQ 143
F NH I+G+ + ++ M H + V C EI + H + + + Q+F + +Q
Sbjct: 290 FSNHGHILGVAINQSINEDMTHYAEVAVSTYESCIEIIEKFHAIAYEGSILQLFIKQPVQ 349
Query: 142 SLSFCWLL*YHP 107
FC L HP
Sbjct: 350 --GFCSNLLNHP 359
>SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 684
Score = 25.4 bits (53), Expect = 3.4
Identities = 13/46 (28%), Positives = 20/46 (43%)
Frame = +1
Query: 88 DQIEMALDDIIKANKKTRIGGGAGRKFDANKRTGRGGGAGFRSNRS 225
D+ + A DD+ K N+ G + A TG + R+N S
Sbjct: 603 DETDAAKDDLFKVNESIETNGHENFQKQALTSTGEQSSSNSRANPS 648
>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 503
Score = 25.0 bits (52), Expect = 4.5
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2
Query: 242 VEIVGHNEIYQLFEGNS 292
+E+ GH I Q+FEG S
Sbjct: 64 LEVAGHKAIVQVFEGTS 80
>SPBC16C6.03c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 158
Score = 24.6 bits (51), Expect = 5.9
Identities = 15/48 (31%), Positives = 22/48 (45%), Gaps = 3/48 (6%)
Frame = +1
Query: 124 ANKKTRIGGGAGRKFDAN---KRTGRGGGAGFRSNRSGGVQRGRNRGA 258
+++ +G R+ D + K T RG G G + GG G RGA
Sbjct: 36 SSETENVGRHGKRRMDQDLSFKPTKRGSGRGRGRSFRGGSHAGNTRGA 83
>SPBC409.09c |mis13|cnl1|kinetochore protein
Mis13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 329
Score = 24.6 bits (51), Expect = 5.9
Identities = 13/43 (30%), Positives = 20/43 (46%), Gaps = 2/43 (4%)
Frame = +1
Query: 94 IEMALDD--IIKANKKTRIGGGAGRKFDANKRTGRGGGAGFRS 216
+E+ + D I+K NK+ R G G D + G GF +
Sbjct: 43 VELPVSDTPIVKRNKELRKGKGRRSSLDQRGKRASSIGTGFEA 85
>SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2493
Score = 24.6 bits (51), Expect = 5.9
Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +2
Query: 164 NLMQTKEQDVEAVLDFVATALVVYNEV-EIVGHN 262
NL++T ++D+ + F + AL V NE+ EI N
Sbjct: 761 NLLKTNQEDLSNLPTFRSQALRVLNELPEIASRN 794
>SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 24.2 bits (50), Expect = 7.8
Identities = 12/23 (52%), Positives = 12/23 (52%)
Frame = +1
Query: 187 GRGGGAGFRSNRSGGVQRGRNRG 255
GRG G G R G RGR RG
Sbjct: 140 GRGRGRGRGRGRGRGGYRGRARG 162
>SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding
protein Ssb2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 279
Score = 24.2 bits (50), Expect = 7.8
Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = +1
Query: 151 GAGRKFDANKRTGRGGGAGFRS-NRSGGVQRGRNRGA 258
G G F++ G GGGAGF ++S R +GA
Sbjct: 10 GYGPDFNSAFSPGMGGGAGFNEYDQSSQPSVDRQQGA 46
>SPAC1786.04 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 111
Score = 24.2 bits (50), Expect = 7.8
Identities = 7/9 (77%), Positives = 8/9 (88%)
Frame = +2
Query: 80 PWLIKLKWH 106
PWLI+ KWH
Sbjct: 76 PWLIRQKWH 84
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,674,163
Number of Sequences: 5004
Number of extensions: 31924
Number of successful extensions: 83
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 74
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 80
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 138190552
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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