BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20467 (832 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 31 0.15 SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo... 31 0.15 SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo... 30 0.46 SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 28 1.9 SPAC17A5.11 |rec12|spo11|endonuclease Rec12|Schizosaccharomyces ... 28 1.9 SPAC20G4.08 ||SPAC4F10.01|sequence orphan|Schizosaccharomyces po... 27 4.3 SPBC1778.04 |spo6||Spo4-Spo6 kinase complex regulatory subunit S... 26 5.7 SPAC6C3.04 |cit1||citrate synthase|Schizosaccharomyces pombe|chr... 26 7.5 >SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 242 Score = 31.5 bits (68), Expect = 0.15 Identities = 18/56 (32%), Positives = 26/56 (46%) Frame = +3 Query: 498 SEVRAGPNLTVADLSLIASVSSLEASDIDFKKYANVKRWYETVKSTAPGYQEANEK 665 +E AG T AD + + + + + K Y N+KRW V S P + A EK Sbjct: 158 NEYFAGEQFTAADPQMCFPIFAAQRDYLSQKPYKNIKRWMRVV-SDRPACRIAAEK 212 >SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharomyces pombe|chr 3|||Manual Length = 229 Score = 31.5 bits (68), Expect = 0.15 Identities = 13/21 (61%), Positives = 15/21 (71%) Frame = +2 Query: 179 EQLKPEYLKLNPQHTVPTLVD 241 EQ PE+L LNP VPTL+D Sbjct: 40 EQKSPEHLALNPNGRVPTLID 60 Score = 28.3 bits (60), Expect = 1.4 Identities = 24/89 (26%), Positives = 41/89 (46%), Gaps = 5/89 (5%) Frame = +1 Query: 256 WESRAIITYLVNKYAKGSSL-YPEDPKARVLVDQRLYFDI---GTLYQRFSDY-FYPQVF 420 WES AI+ YL +KY + P D V Q L+F G ++ + + Y Q Sbjct: 69 WESDAILIYLADKYDTERKISLPRDHPEYYKVIQYLFFQASGQGIIWGQAGWFSVYHQEL 128 Query: 421 AGAPADKAKNEKVQEALQLLDKFLEGQKY 507 + + +NE ++ L +L+ L+ + Y Sbjct: 129 VISAITRYRNE-IKRVLGVLEDILKDRDY 156 >SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharomyces pombe|chr 3|||Manual Length = 230 Score = 29.9 bits (64), Expect = 0.46 Identities = 14/22 (63%), Positives = 15/22 (68%) Frame = +2 Query: 176 GEQLKPEYLKLNPQHTVPTLVD 241 GEQ E+L LNP VPTLVD Sbjct: 39 GEQKCKEHLALNPNGRVPTLVD 60 Score = 29.5 bits (63), Expect = 0.61 Identities = 26/91 (28%), Positives = 49/91 (53%), Gaps = 7/91 (7%) Frame = +1 Query: 256 WESRAIITYLVNKY--AKGSSLYPEDPKARVLVDQRLYFDI---GTLYQR--FSDYFYPQ 414 WES AI+ YL +KY + SL +DP+ L+ Q L+F G ++ + + ++F+ + Sbjct: 69 WESDAILIYLADKYDTDRKISLSFDDPEYYKLI-QYLFFQASGQGVIWGQAGWFNFFHHE 127 Query: 415 VFAGAPADKAKNEKVQEALQLLDKFLEGQKY 507 A + +NE ++ L +L+ L+ + Y Sbjct: 128 PVVSA-VTRYRNE-IKRVLGVLEDILKDRDY 156 >SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1469 Score = 27.9 bits (59), Expect = 1.9 Identities = 12/35 (34%), Positives = 22/35 (62%) Frame = -3 Query: 713 SLFKHAIDKSLECLQSFLVGLLVTRSCTLNRLVPS 609 S +K++++ L CL +L+ +TRS T+ + V S Sbjct: 51 SWYKYSLESGLVCLYLYLIYRWITRSFTVKQTVGS 85 >SPAC17A5.11 |rec12|spo11|endonuclease Rec12|Schizosaccharomyces pombe|chr 1|||Manual Length = 345 Score = 27.9 bits (59), Expect = 1.9 Identities = 23/72 (31%), Positives = 33/72 (45%), Gaps = 3/72 (4%) Frame = +1 Query: 190 TRIFEVEPSTHGPDPSRRWSFNWESRAIITYLVNKYAKGSSLYPEDPKARVLVDQ---RL 360 TR F V+ + PD F+W+ + Y KY GS+ Y +P +++ Q L Sbjct: 208 TRKFLVKLAKALPDAKFFGIFDWDPHGLCIYSCFKY--GSNAYSHEPHSQLRNLQLLGPL 265 Query: 361 YFDIGTLYQRFS 396 Y DI Q FS Sbjct: 266 YEDIFNKNQEFS 277 >SPAC20G4.08 ||SPAC4F10.01|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1076 Score = 26.6 bits (56), Expect = 4.3 Identities = 13/33 (39%), Positives = 21/33 (63%) Frame = +3 Query: 600 NVKRWYETVKSTAPGYQEANEKGLEAFKGLVNS 698 +V + YE+++S Y+ ANEK EA +V+S Sbjct: 739 SVHKNYESLRSQLLEYKAANEKHTEAILSVVSS 771 >SPBC1778.04 |spo6||Spo4-Spo6 kinase complex regulatory subunit Spo6|Schizosaccharomyces pombe|chr 2|||Manual Length = 474 Score = 26.2 bits (55), Expect = 5.7 Identities = 19/62 (30%), Positives = 29/62 (46%) Frame = -3 Query: 770 QVYSSDVILNKFSFRVDKCSLFKHAIDKSLECLQSFLVGLLVTRSCTLNRLVPSLNVGVF 591 +++ D +LN+ F + ++ S CLQS L G V + + VPS NV F Sbjct: 194 KIWLLDKLLNRILFTLLNSDSL---VNTSASCLQSLLDGEKVYGTSDKDFYVPSKNVEYF 250 Query: 590 LE 585 E Sbjct: 251 RE 252 >SPAC6C3.04 |cit1||citrate synthase|Schizosaccharomyces pombe|chr 1|||Manual Length = 473 Score = 25.8 bits (54), Expect = 7.5 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = -2 Query: 327 VFGVQTAALSVLVHQVGDDGAGLPVERPSS 238 +FGV + L V + D GLP+ERP S Sbjct: 432 LFGV-SRTLGVASQLIWDRALGLPIERPKS 460 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,028,007 Number of Sequences: 5004 Number of extensions: 58939 Number of successful extensions: 178 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 172 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 178 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 408446760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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