Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20459
(720 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 154 1e-38
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 151 1e-37
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 95 9e-21
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 66 4e-12
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.2
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.2
SPAPB1A11.01 ||SPAPB24D3.11|membrane transporter|Schizosaccharom... 25 8.2
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.2
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 154 bits (373), Expect = 1e-38
Identities = 65/93 (69%), Positives = 79/93 (84%)
Frame = +2
Query: 230 FQHFLQRDRAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 409
F F GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 410 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSF 508
T+GKE+VD V D+IR++AD C+GLQGFL+FHSF
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSF 141
Score = 101 bits (242), Expect = 1e-22
Identities = 43/60 (71%), Positives = 55/60 (91%)
Frame = +1
Query: 511 GGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAF 690
GGTGSGF +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC F
Sbjct: 143 GGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTF 202
Score = 90.6 bits (215), Expect = 2e-19
Identities = 39/56 (69%), Positives = 43/56 (76%)
Frame = +3
Query: 87 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 254
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSET
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSET 56
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 151 bits (365), Expect = 1e-37
Identities = 62/93 (66%), Positives = 79/93 (84%)
Frame = +2
Query: 230 FQHFLQRDRAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 409
F F GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY
Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112
Query: 410 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSF 508
T+GKE++D VL+RIR++AD C+GLQGFL+FHSF
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSF 145
Score = 103 bits (246), Expect = 3e-23
Identities = 43/60 (71%), Positives = 55/60 (91%)
Frame = +1
Query: 511 GGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAF 690
GGTGSG +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC F
Sbjct: 147 GGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTF 206
Score = 84.6 bits (200), Expect = 1e-17
Identities = 43/61 (70%), Positives = 45/61 (73%), Gaps = 5/61 (8%)
Frame = +3
Query: 87 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 251
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 252 T 254
T
Sbjct: 60 T 60
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 95.1 bits (226), Expect = 9e-21
Identities = 42/82 (51%), Positives = 57/82 (69%)
Frame = +2
Query: 260 GKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLV 439
GK+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D V
Sbjct: 57 GKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAV 116
Query: 440 LDRIRKLADQCTGLQGFLIFHS 505
LD +R+ A+ C LQGF + HS
Sbjct: 117 LDVVRREAEACDALQGFQLTHS 138
Score = 60.1 bits (139), Expect = 3e-10
Identities = 27/62 (43%), Positives = 40/62 (64%)
Frame = +1
Query: 505 LRGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDC 684
L GGTGSG +LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD
Sbjct: 139 LGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDE 198
Query: 685 AF 690
F
Sbjct: 199 TF 200
Score = 34.7 bits (76), Expect = 0.013
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +3
Query: 87 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDG 188
MRE + + GQ G Q+G A W EHG+ G
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAG 34
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 66.5 bits (155), Expect = 4e-12
Identities = 34/91 (37%), Positives = 55/91 (60%), Gaps = 2/91 (2%)
Frame = +2
Query: 239 FLQRDRAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 412
F Q D +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+
Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 413 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHS 505
+ I + ++D I + AD L+GF + HS
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHS 140
Score = 50.0 bits (114), Expect = 3e-07
Identities = 25/50 (50%), Positives = 34/50 (68%), Gaps = 1/50 (2%)
Frame = +1
Query: 505 LRGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSIL 651
+ GGTGSG S L+ERL+ Y KK ++++P Q VS VV+PYNS+L
Sbjct: 141 IAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLL 190
Score = 49.6 bits (113), Expect = 4e-07
Identities = 24/56 (42%), Positives = 34/56 (60%)
Frame = +3
Query: 90 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETE 257
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF +++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSD 56
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 185 WPDAHRQDHRGWRRFFQHFLQR 250
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.2
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = +1
Query: 166 STASSLMARCPQTRPSGVETILSTLSSARPS 258
ST SSL + ++PS T ST SSA P+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPT 203
>SPAPB1A11.01 ||SPAPB24D3.11|membrane
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 495
Score = 25.4 bits (53), Expect = 8.2
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +3
Query: 498 STPSWRYRLWVHFLIDGASL 557
S+ SWR+ W+ ++ G SL
Sbjct: 190 SSISWRWEFWILLMLSGVSL 209
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.2
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +1
Query: 76 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 198
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,969,268
Number of Sequences: 5004
Number of extensions: 61059
Number of successful extensions: 189
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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