Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20452
(484 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 140 1e-34
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 140 1e-34
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 140 1e-34
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 79 3e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 73 3e-14
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 47 2e-06
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.004
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.004
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 32 0.052
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 29 0.37
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 26 2.6
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 26 2.6
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy... 25 4.5
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 25 4.5
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 4.5
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 6.0
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 6.0
SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 25 6.0
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe... 25 7.9
SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 7.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 140 bits (338), Expect = 1e-34
Identities = 63/68 (92%), Positives = 66/68 (97%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 83.0 bits (196), Expect = 2e-17
Identities = 37/40 (92%), Positives = 38/40 (95%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 374
RGITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQ
Sbjct: 69 RGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQ 108
Score = 68.5 bits (160), Expect = 5e-13
Identities = 30/37 (81%), Positives = 34/37 (91%)
Frame = +2
Query: 374 ADCAVLIVAAGTGEFEAGISKNGQTREHALLDFTLGV 484
ADCA+LI+ GTGEFEAGISK+GQTREHALL +TLGV
Sbjct: 109 ADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGV 145
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 140 bits (338), Expect = 1e-34
Identities = 63/68 (92%), Positives = 66/68 (97%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 83.0 bits (196), Expect = 2e-17
Identities = 37/40 (92%), Positives = 38/40 (95%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 374
RGITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQ
Sbjct: 69 RGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQ 108
Score = 68.9 bits (161), Expect = 4e-13
Identities = 31/37 (83%), Positives = 34/37 (91%)
Frame = +2
Query: 374 ADCAVLIVAAGTGEFEAGISKNGQTREHALLDFTLGV 484
ADCAVLI+ GTGEFEAGISK+GQTREHALL +TLGV
Sbjct: 109 ADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGV 145
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 140 bits (338), Expect = 1e-34
Identities = 63/68 (92%), Positives = 66/68 (97%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 83.0 bits (196), Expect = 2e-17
Identities = 37/40 (92%), Positives = 38/40 (95%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 374
RGITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQ
Sbjct: 69 RGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQ 108
Score = 68.5 bits (160), Expect = 5e-13
Identities = 30/37 (81%), Positives = 34/37 (91%)
Frame = +2
Query: 374 ADCAVLIVAAGTGEFEAGISKNGQTREHALLDFTLGV 484
ADCA+LI+ GTGEFEAGISK+GQTREHALL +TLGV
Sbjct: 109 ADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGV 145
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 79.4 bits (187), Expect = 3e-16
Identities = 34/64 (53%), Positives = 47/64 (73%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 243
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 244 AERD 255
ER+
Sbjct: 296 EERE 299
Score = 48.0 bits (109), Expect = 7e-07
Identities = 21/37 (56%), Positives = 27/37 (72%)
Frame = +2
Query: 374 ADCAVLIVAAGTGEFEAGISKNGQTREHALLDFTLGV 484
AD VL+++A GEFEAG + GQTREHA+L T G+
Sbjct: 340 ADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGI 376
Score = 41.5 bits (93), Expect = 6e-05
Identities = 16/40 (40%), Positives = 26/40 (65%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 374
+G T+++ FET +++DAPGH+ ++ NMI G SQ
Sbjct: 300 KGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQ 339
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 72.5 bits (170), Expect = 3e-14
Identities = 31/63 (49%), Positives = 44/63 (69%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 243
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 244 AER 252
ER
Sbjct: 235 EER 237
Score = 48.8 bits (111), Expect = 4e-07
Identities = 22/39 (56%), Positives = 26/39 (66%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS 371
RG+T+D+A FE+ K I DAPGHRDFI MI G S
Sbjct: 239 RGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGAS 277
Score = 43.2 bits (97), Expect = 2e-05
Identities = 21/38 (55%), Positives = 25/38 (65%)
Frame = +2
Query: 371 SADCAVLIVAAGTGEFEAGISKNGQTREHALLDFTLGV 484
SAD AVL+V + FE G +NGQTREHA L LG+
Sbjct: 278 SADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGI 315
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 46.8 bits (106), Expect = 2e-06
Identities = 20/41 (48%), Positives = 28/41 (68%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQL 377
RGITI A ++ET+ + +D PGH D+IKNMITG + +
Sbjct: 100 RGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATM 140
Score = 35.1 bits (77), Expect = 0.006
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +1
Query: 58 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 144
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.004
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 147
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.004
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 147
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 31.9 bits (69), Expect = 0.052
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDF 344
RGITI+ A F + +ID PGH DF
Sbjct: 77 RGITINSAAISFTWRNQRINLIDTPGHADF 106
Score = 28.7 bits (61), Expect = 0.49
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCG 147
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.1 bits (62), Expect = 0.37
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
Score = 26.2 bits (55), Expect = 2.6
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +3
Query: 288 FETSKYYVTIIDAPGHRDF 344
FE S Y + IID PGH DF
Sbjct: 141 FEKS-YNINIIDTPGHIDF 158
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 26.2 bits (55), Expect = 2.6
Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Frame = +3
Query: 261 ITIDIALWKFETSKYYV--TIIDAPGHRDFIKN 353
+ I+I + E +++ T+ID PG DFI N
Sbjct: 71 VEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 26.2 bits (55), Expect = 2.6
Identities = 12/34 (35%), Positives = 18/34 (52%), Gaps = 4/34 (11%)
Frame = +3
Query: 255 RGITIDIA----LWKFETSKYYVTIIDAPGHRDF 344
RGIT+ ++ + Y + +ID PGH DF
Sbjct: 104 RGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDF 137
Score = 24.6 bits (51), Expect = 7.9
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGIDK 159
N VI H+D GKST + ++ G I++
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINE 87
>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 384
Score = 25.4 bits (53), Expect = 4.5
Identities = 18/60 (30%), Positives = 27/60 (45%)
Frame = -3
Query: 326 SINDGNIVLASFELPESNIDCDTTSRSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSIP 147
S++ + L S+ P S T A ++YL+ PL S S S + +R SIP
Sbjct: 305 SVDSASTALNSYSTPNSVSRKLTNLTPAARRLVARSYLRSPLHGSSPSASRHTALRTSIP 364
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 25.4 bits (53), Expect = 4.5
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -1
Query: 238 VCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWWWT 110
V +PK I I++ F P S S C+Y S D W T
Sbjct: 31 VLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRT 73
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 25.4 bits (53), Expect = 4.5
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +3
Query: 303 YYVTIIDAPGHRDFIKNMITGTSQL 377
Y + +ID+PGH DF + ++ S+L
Sbjct: 94 YLINLIDSPGHVDF-SSEVSSASRL 117
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 6.0
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +1
Query: 46 PKMGKEKTHINIVVIGHVDSGKST 117
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 6.0
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -1
Query: 193 PGPPSRTSRWYVCQYHHIC 137
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
>SPAC9G1.10c |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1191
Score = 25.0 bits (52), Expect = 6.0
Identities = 16/38 (42%), Positives = 21/38 (55%)
Frame = +3
Query: 366 TSQLIALCSS*LPVPVNSKLVSLRTVKPVSMPCSISPS 479
TS+ ++ S L P ++ SL T KPVSMP S S
Sbjct: 378 TSENLSSKPSPLFPPPPPRVKSLATNKPVSMPVSTEQS 415
>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 734
Score = 24.6 bits (51), Expect = 7.9
Identities = 9/25 (36%), Positives = 14/25 (56%)
Frame = -2
Query: 477 RVKSSKACSRV*PFLEIPASNSPVP 403
R S +C+R+ P + +P S P P
Sbjct: 601 RTLQSLSCARIRPLVMVPKSKKPSP 625
>SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 96
Score = 24.6 bits (51), Expect = 7.9
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = -3
Query: 362 CDHVLDEISVSRSINDGNIVLASFEL 285
CDH+L IS SR G + FEL
Sbjct: 23 CDHLLQYISNSRDSKAGLKIFLVFEL 48
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,926,098
Number of Sequences: 5004
Number of extensions: 36926
Number of successful extensions: 151
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 186042952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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