Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20393
(583 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q6GLJ2 Cluster: LOC443718 protein; n=4; Tetrapoda|Rep: ... 73 4e-12
UniRef50_Q3B726 Cluster: DNA-directed RNA polymerase I subunit R... 70 3e-11
UniRef50_UPI00005A2C8E Cluster: PREDICTED: similar to TWIST neig... 70 5e-11
UniRef50_Q4RKE7 Cluster: Chromosome 21 SCAF15029, whole genome s... 65 1e-09
UniRef50_Q6PHG8 Cluster: DNA-directed RNA polymerase I subunit R... 64 2e-09
UniRef50_A7RZD8 Cluster: Predicted protein; n=1; Nematostella ve... 59 7e-08
UniRef50_Q16XZ3 Cluster: Putative uncharacterized protein; n=1; ... 53 6e-06
UniRef50_Q9VM72 Cluster: CG13773-PA; n=2; Sophophora|Rep: CG1377... 51 2e-05
UniRef50_Q7PV20 Cluster: ENSANGP00000016750; n=1; Anopheles gamb... 49 7e-05
UniRef50_O43036 Cluster: DNA-directed RNA polymerase I subunit R... 40 0.057
UniRef50_Q6C2P5 Cluster: Yarrowia lipolytica chromosome F of str... 39 0.075
UniRef50_Q6BX66 Cluster: Similar to CA2272|CaRPA43 Candida albic... 36 0.53
UniRef50_A3LV72 Cluster: DNA-dependent RNA polymerase I subunit ... 36 0.92
UniRef50_A6SBP0 Cluster: Putative uncharacterized protein; n=2; ... 35 1.2
UniRef50_Q00W27 Cluster: DNA polymerase epsilon, subunit C; n=3;... 34 2.1
UniRef50_Q55FA4 Cluster: RNA polymerase I subunit; n=1; Dictyost... 34 2.1
UniRef50_Q4PEV0 Cluster: Putative uncharacterized protein; n=1; ... 33 3.7
UniRef50_UPI000023E987 Cluster: hypothetical protein FG04470.1; ... 33 6.5
>UniRef50_Q6GLJ2 Cluster: LOC443718 protein; n=4; Tetrapoda|Rep:
LOC443718 protein - Xenopus laevis (African clawed frog)
Length = 477
Score = 73.3 bits (172), Expect = 4e-12
Identities = 34/82 (41%), Positives = 48/82 (58%)
Frame = +2
Query: 260 VSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRV 439
V+Y N ++ +G I +D IH V+AD+ IF+P G L G+VNK TH+ LVH
Sbjct: 92 VAYDNIKLVGELGDIYDDLGHIHINVEADFVIFKPDCGQTLVGIVNKVAPTHIGCLVHGC 151
Query: 440 FNVVIPQPTEEPGDKWIGSSVK 505
FN IP+P + P + W+ VK
Sbjct: 152 FNASIPKPPKMPIENWLRIGVK 173
Score = 32.3 bits (70), Expect = 8.6
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = +3
Query: 96 ELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKVGKFDKELNG 254
E +L + + SCLVV+ ++LAL P L + I L+ ++ K+ + LNG
Sbjct: 37 EACELVSCRYSCLVVETHRRHLALSPKFLRKKRSGIQEQLNTELLKYSERLNG 89
>UniRef50_Q3B726 Cluster: DNA-directed RNA polymerase I subunit
RPA43; n=16; Amniota|Rep: DNA-directed RNA polymerase I
subunit RPA43 - Homo sapiens (Human)
Length = 338
Score = 70.1 bits (164), Expect = 3e-11
Identities = 30/76 (39%), Positives = 46/76 (60%)
Frame = +2
Query: 260 VSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRV 439
++Y N ++ +G I +D IH ++AD+ IF P G KL G+VNK S+H+ LVH
Sbjct: 91 IAYDNIKVVGELGDIYDDQGHIHLNIEADFVIFCPEPGQKLMGIVNKVSSSHIGCLVHGC 150
Query: 440 FNVVIPQPTEEPGDKW 487
FN IP+P + ++W
Sbjct: 151 FNASIPKPEQLSAEQW 166
>UniRef50_UPI00005A2C8E Cluster: PREDICTED: similar to TWIST
neighbor; n=1; Canis lupus familiaris|Rep: PREDICTED:
similar to TWIST neighbor - Canis familiaris
Length = 423
Score = 69.7 bits (163), Expect = 5e-11
Identities = 30/76 (39%), Positives = 46/76 (60%)
Frame = +2
Query: 260 VSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRV 439
++Y N ++ +G I +D IH ++AD+ IF P G KL G+VNK S+H+ LVH
Sbjct: 169 IAYDNIKVVGELGDIYDDQGHIHLNIEADFVIFCPEPGKKLMGIVNKVSSSHIGCLVHGC 228
Query: 440 FNVVIPQPTEEPGDKW 487
FN IP+P + ++W
Sbjct: 229 FNASIPKPEQMSTEQW 244
>UniRef50_Q4RKE7 Cluster: Chromosome 21 SCAF15029, whole genome
shotgun sequence; n=3; Tetraodontidae|Rep: Chromosome 21
SCAF15029, whole genome shotgun sequence - Tetraodon
nigroviridis (Green puffer)
Length = 352
Score = 65.3 bits (152), Expect = 1e-09
Identities = 32/76 (42%), Positives = 42/76 (55%)
Frame = +2
Query: 260 VSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRV 439
++Y N IL G I +D +H V AD+ +FQP G KL G VNK +H+ LVH
Sbjct: 107 LAYDNIRILGQYGDIVDDGGYVHMDVIADFIVFQPQKGQKLLGKVNKLGVSHVGCLVHGC 166
Query: 440 FNVVIPQPTEEPGDKW 487
FN IP+P P + W
Sbjct: 167 FNASIPKPNLVPMETW 182
>UniRef50_Q6PHG8 Cluster: DNA-directed RNA polymerase I subunit
RPA43; n=3; Danio rerio|Rep: DNA-directed RNA polymerase
I subunit RPA43 - Danio rerio (Zebrafish) (Brachydanio
rerio)
Length = 391
Score = 64.1 bits (149), Expect = 2e-09
Identities = 28/81 (34%), Positives = 47/81 (58%)
Frame = +2
Query: 260 VSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRV 439
V+Y N ++ G I +D IHF ++A + IF+P G++L G++NK ++H+ LVH
Sbjct: 99 VAYDNIKVVGQHGNIYDDQGFIHFNIEASFVIFRPKNGSRLMGVINKMGASHVGCLVHGC 158
Query: 440 FNVVIPQPTEEPGDKWIGSSV 502
FN + +P D+W S +
Sbjct: 159 FNASVMKPNALTSDQWRDSGL 179
>UniRef50_A7RZD8 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 159
Score = 59.3 bits (137), Expect = 7e-08
Identities = 29/79 (36%), Positives = 42/79 (53%)
Frame = +2
Query: 242 RVERFLVSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLS 421
++ +V+Y +LQ G I +++ +HF Q DY F+P IG KL G+VNK H+
Sbjct: 44 KLRAVIVAYDKVTLLQRRGNIIDESPFLHFDAQVDYITFKPEIGNKLIGVVNKFGYDHIG 103
Query: 422 VLVHRVFNVVIPQPTEEPG 478
LVH FN I + G
Sbjct: 104 CLVHGCFNASIAKSRTSNG 122
>UniRef50_Q16XZ3 Cluster: Putative uncharacterized protein; n=1;
Aedes aegypti|Rep: Putative uncharacterized protein -
Aedes aegypti (Yellowfever mosquito)
Length = 571
Score = 52.8 bits (121), Expect = 6e-06
Identities = 22/70 (31%), Positives = 43/70 (61%)
Frame = +2
Query: 245 VERFLVSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSV 424
V+ ++++KN IL + IR ++ IH +V +++++F+P GA + G + +LS
Sbjct: 66 VDGIILAFKNTKILSPLSAIRPNSVRIHVKVSSNFYVFRPISGATIEGTIKYVSKNYLSA 125
Query: 425 LVHRVFNVVI 454
+++ VFNV I
Sbjct: 126 VIYGVFNVTI 135
Score = 42.7 bits (96), Expect = 0.006
Identities = 20/62 (32%), Positives = 36/62 (58%)
Frame = +3
Query: 69 SKIIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKVGKFDKEL 248
+K K+ + EL+ D NSC+ + T +++ P LG+I E + L K+GK+D+ +
Sbjct: 7 TKYAKYTVEELESYVADPNSCVQKLEWTNLVSVLPKYLGDICEGVKACLTQKIGKYDRTV 66
Query: 249 NG 254
+G
Sbjct: 67 DG 68
>UniRef50_Q9VM72 Cluster: CG13773-PA; n=2; Sophophora|Rep:
CG13773-PA - Drosophila melanogaster (Fruit fly)
Length = 253
Score = 51.2 bits (117), Expect = 2e-05
Identities = 22/70 (31%), Positives = 41/70 (58%)
Frame = +2
Query: 245 VERFLVSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSV 424
++ ++ KN +L +R D+ +H + AD+++F+P GA L+G+V H+S
Sbjct: 66 LDGIVLGIKNIKVLGQTAGLRADDPTMHLVINADFYVFRPKAGAILSGVVRHISRHHVSA 125
Query: 425 LVHRVFNVVI 454
+++RVFN I
Sbjct: 126 VIYRVFNTSI 135
Score = 43.2 bits (97), Expect = 0.005
Identities = 23/65 (35%), Positives = 37/65 (56%), Gaps = 1/65 (1%)
Frame = +3
Query: 63 IMSKIIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLL-DYKVGKFD 239
I+ K IKF ++EL+ A+ SC+ +LA+ P+ + N K ++ LL KVG +D
Sbjct: 4 ILQKYIKFSVKELENYASSPESCVRCITTDMHLAMGPYGMANFKHALHELLIRTKVGFYD 63
Query: 240 KELNG 254
L+G
Sbjct: 64 SGLDG 68
>UniRef50_Q7PV20 Cluster: ENSANGP00000016750; n=1; Anopheles gambiae
str. PEST|Rep: ENSANGP00000016750 - Anopheles gambiae
str. PEST
Length = 246
Score = 49.2 bits (112), Expect = 7e-05
Identities = 20/54 (37%), Positives = 33/54 (61%)
Frame = +2
Query: 293 VGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 454
+ +R D+ +H + DY++FQP IG+ L G VN +S +++RVFNV +
Sbjct: 82 LSALRLDSPYLHVRTTIDYYVFQPRIGSTLRGTVNYVSKNFVSAVIYRVFNVTV 135
Score = 37.1 bits (82), Expect = 0.30
Identities = 17/63 (26%), Positives = 35/63 (55%)
Frame = +3
Query: 66 MSKIIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKVGKFDKE 245
++K KF EL++ D SC+ ++ + L+L+P +I + + + K+G++ +
Sbjct: 6 IAKYTKFSAEELQKSTADPLSCVAAVQLNEILSLRPRDCEHIVKGVKRCVTDKIGRYHAK 65
Query: 246 LNG 254
LNG
Sbjct: 66 LNG 68
>UniRef50_O43036 Cluster: DNA-directed RNA polymerase I subunit
RPA43; n=1; Schizosaccharomyces pombe|Rep: DNA-directed
RNA polymerase I subunit RPA43 - Schizosaccharomyces
pombe (Fission yeast)
Length = 173
Score = 39.5 bits (88), Expect = 0.057
Identities = 23/83 (27%), Positives = 40/83 (48%)
Frame = +2
Query: 242 RVERFLVSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLS 421
R+ +++Y N L+ + D+ V+ D +F P G L G +N +H+
Sbjct: 43 RINGIVLAYDNIRFLEKSAKVMYDSPFSFIWVRVDVLVFSPKKGDCLEGKINLVSPSHIG 102
Query: 422 VLVHRVFNVVIPQPTEEPGDKWI 490
+L+ +FN IP+ + P D WI
Sbjct: 103 LLILGIFNASIPRKS-IPKD-WI 123
>UniRef50_Q6C2P5 Cluster: Yarrowia lipolytica chromosome F of strain
CLIB122 of Yarrowia lipolytica; n=1; Yarrowia
lipolytica|Rep: Yarrowia lipolytica chromosome F of
strain CLIB122 of Yarrowia lipolytica - Yarrowia
lipolytica (Candida lipolytica)
Length = 288
Score = 39.1 bits (87), Expect = 0.075
Identities = 16/54 (29%), Positives = 29/54 (53%)
Frame = +2
Query: 293 VGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 454
+G N++ + D +++P +G +L G VN + +H+ +LVH FN I
Sbjct: 98 MGKFVNESPFTFMWISTDLLVWKPQVGDRLEGYVNMQTPSHIGLLVHDTFNCSI 151
>UniRef50_Q6BX66 Cluster: Similar to CA2272|CaRPA43 Candida albicans
CaRPA43 DNA-directed RNA polymerase I; n=3;
Saccharomycetales|Rep: Similar to CA2272|CaRPA43 Candida
albicans CaRPA43 DNA-directed RNA polymerase I -
Debaryomyces hansenii (Yeast) (Torulaspora hansenii)
Length = 311
Score = 36.3 bits (80), Expect = 0.53
Identities = 14/40 (35%), Positives = 24/40 (60%)
Frame = +2
Query: 335 VQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 454
V D+ I++P +G L G + + ++H+ +LVH FN I
Sbjct: 122 VSVDFLIWRPQVGDTLEGYIYMQTASHIGLLVHDTFNASI 161
>UniRef50_A3LV72 Cluster: DNA-dependent RNA polymerase I subunit
A43; n=2; Pichia|Rep: DNA-dependent RNA polymerase I
subunit A43 - Pichia stipitis (Yeast)
Length = 307
Score = 35.5 bits (78), Expect = 0.92
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +2
Query: 335 VQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 454
+ D+ I++P +G L G + + ++HL +L+H FN I
Sbjct: 122 ITVDFLIWRPQVGDVLEGYIYMQTASHLGLLIHDTFNASI 161
>UniRef50_A6SBP0 Cluster: Putative uncharacterized protein; n=2;
Sclerotiniaceae|Rep: Putative uncharacterized protein -
Botryotinia fuckeliana B05.10
Length = 323
Score = 35.1 bits (77), Expect = 1.2
Identities = 17/58 (29%), Positives = 30/58 (51%)
Frame = +2
Query: 335 VQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVIPQPTEEPGDKWIGSSVKE 508
V A++ +F+P GA L G +N + HL ++ +FN I + +W+G +E
Sbjct: 150 VTAEFLLFKPEKGAWLEGYINLQNEGHLGLVCWNLFNASIERARLPKEWRWVGVEDQE 207
>UniRef50_Q00W27 Cluster: DNA polymerase epsilon, subunit C; n=3;
Ostreococcus|Rep: DNA polymerase epsilon, subunit C -
Ostreococcus tauri
Length = 569
Score = 34.3 bits (75), Expect = 2.1
Identities = 18/53 (33%), Positives = 27/53 (50%)
Frame = +2
Query: 296 GTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 454
G + + + + V+AD +F P G++L G VNK + LV FNV I
Sbjct: 101 GRVLHASGHVDVCVEADCDVFTPVRGSRLVGTVNKIAHDFIGALVLDKFNVAI 153
>UniRef50_Q55FA4 Cluster: RNA polymerase I subunit; n=1;
Dictyostelium discoideum AX4|Rep: RNA polymerase I
subunit - Dictyostelium discoideum AX4
Length = 356
Score = 34.3 bits (75), Expect = 2.1
Identities = 16/68 (23%), Positives = 33/68 (48%)
Frame = +2
Query: 257 LVSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHR 436
++SY + I D+ + + + +F+P+ L G+V + +TH+S+LV
Sbjct: 61 IISYYDVIHTDKEAKIFYDSPYLGMNIHVKFLVFKPFKDQILNGVVKRVSTTHISLLVFG 120
Query: 437 VFNVVIPQ 460
+ IP+
Sbjct: 121 TISASIPK 128
>UniRef50_Q4PEV0 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 364
Score = 33.5 bits (73), Expect = 3.7
Identities = 18/67 (26%), Positives = 33/67 (49%)
Frame = +2
Query: 257 LVSYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHR 436
L+++ LQ+ D+A V + ++ P IG L G + +H+S+L++
Sbjct: 117 LITHSPTRFLQDTAVFSADSAFATASVGFECVVWTPRIGQMLEGNICLSSPSHVSLLLYG 176
Query: 437 VFNVVIP 457
+FN IP
Sbjct: 177 LFNASIP 183
>UniRef50_UPI000023E987 Cluster: hypothetical protein FG04470.1;
n=1; Gibberella zeae PH-1|Rep: hypothetical protein
FG04470.1 - Gibberella zeae PH-1
Length = 451
Score = 32.7 bits (71), Expect = 6.5
Identities = 18/60 (30%), Positives = 28/60 (46%)
Frame = +2
Query: 335 VQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVIPQPTEEPGDKWIGSSVKEAK 514
+ A+ +F P GA + G VN + H+ V+ FN I P KW+ + EA+
Sbjct: 264 ITAEVDLFVPSRGAWMEGSVNLQTEGHIGVVCFGKFNASIEARRLPPDWKWVPNESPEAQ 323
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 519,603,427
Number of Sequences: 1657284
Number of extensions: 9324200
Number of successful extensions: 18634
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 18270
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18631
length of database: 575,637,011
effective HSP length: 96
effective length of database: 416,537,747
effective search space used: 40404161459
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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