BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20380 (671 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC688.06c |slx4||structure-specific endonuclease subunit |Schi... 30 0.26 SPAC16E8.02 |||DUF962 family protein|Schizosaccharomyces pombe|c... 29 0.61 SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe... 29 0.61 SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 27 3.3 SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 4.3 SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces po... 26 4.3 SPAC10F6.14c |||ABC1 kinase family protein|Schizosaccharomyces p... 26 5.7 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 26 5.7 SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |S... 26 5.7 SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 26 5.7 SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4... 26 5.7 SPAC56F8.07 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 7.5 SPCC61.05 |||S. pombe specific multicopy membrane protein family... 25 7.5 SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase |Schizosacchar... 25 7.5 SPCC1919.12c |||aminopeptidase |Schizosaccharomyces pombe|chr 3|... 25 9.9 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 25 9.9 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.9 >SPAC688.06c |slx4||structure-specific endonuclease subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 30.3 bits (65), Expect = 0.26 Identities = 15/53 (28%), Positives = 26/53 (49%), Gaps = 1/53 (1%) Frame = +2 Query: 500 STQQELVHEDIYSEAHGKLKTIIRDLKKRQIWVFTEKKIFRYKIVR-EERNLW 655 ST+ L +DI ++ H + +++ K +W+ KI Y + EE LW Sbjct: 342 STKTVLEFDDIVTQTHRAISQVVKQAKDNSVWI----KILTYSAIDVEEFQLW 390 >SPAC16E8.02 |||DUF962 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 222 Score = 29.1 bits (62), Expect = 0.61 Identities = 21/56 (37%), Positives = 28/56 (50%), Gaps = 8/56 (14%) Frame = +1 Query: 133 IYQFIGHAIFSENKPSL-----KSVFY-PYLTIPETG--FQEIPSTLKYLSYNITL 276 I QFIGH +F + KP+L +S+F P ETG PS + + NI L Sbjct: 115 ILQFIGHGVFEKRKPALLDNLIQSLFIAPLFAFLETGPFVGYYPSVVSKIRANIKL 170 >SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe|chr 1|||Manual Length = 727 Score = 29.1 bits (62), Expect = 0.61 Identities = 18/42 (42%), Positives = 23/42 (54%) Frame = +3 Query: 315 EQGIFYGQLDPTSQQNSNSVVTQGSLVTYPSGPDGKEIPPLS 440 E G+ G L SQQN+N+ TQ S+ + P E PPLS Sbjct: 534 EGGLLSGALRAPSQQNNNNSNTQHSINLHTIVPSPYE-PPLS 574 >SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 2609 Score = 26.6 bits (56), Expect = 3.3 Identities = 14/56 (25%), Positives = 28/56 (50%), Gaps = 2/56 (3%) Frame = +1 Query: 181 LKSVFYPYLTIPETGFQEIPSTLKYLSYN--ITLIRMKCLKRLPGLPNRVSSMVSW 342 + S F+P L I + GF I + L++ ++L +C ++ G+ + + SW Sbjct: 42 IPSNFFPQLFITDNGFAAIKKAVTKLNFQSLLSLDIFECCLQILGIATSYTILRSW 97 >SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 26.2 bits (55), Expect = 4.3 Identities = 23/84 (27%), Positives = 36/84 (42%) Frame = +1 Query: 382 KARS*PTLRDRTAKKYPHYHLFSRNSTLC*CIQIELK*YLYSTRTCPRGYLLGSSWETQN 561 K++S P LR + + H H S++ + K + C R GSS + Sbjct: 195 KSKSVPRLRGQFMEPVEHNHPLSKSLEEQSSFLEQSKDASSNLTACNRS---GSSLSSNF 251 Query: 562 NNTRLEEKTDLGVYGKKDISLQDR 633 ++RL +KT L K SLQ + Sbjct: 252 YSSRLSKKTSLASLNKSRASLQHK 275 >SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces pombe|chr 1|||Manual Length = 639 Score = 26.2 bits (55), Expect = 4.3 Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 2/56 (3%) Frame = +1 Query: 217 ETGFQEIPSTLKYLSYNITLIRMKCLKRL--PGLPNRVSSMVSWTRHLNRTQILLS 378 E ++I ST + +SY+ +KCL ++ P + SS V T + I++S Sbjct: 196 EYAMKKINSTFQQMSYSKIFREIKCLAKMDHPNVIRYFSSWVESTTEATQMPIVMS 251 >SPAC10F6.14c |||ABC1 kinase family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 535 Score = 25.8 bits (54), Expect = 5.7 Identities = 17/69 (24%), Positives = 32/69 (46%) Frame = +3 Query: 96 KKVLYICDNTNKNLPVHRACHFF*EQTISEVSLLSVFNHSRNRFSGNTFYVKISELQYYF 275 K ++Y+ D ++P++ + E+ SEV + N+S + G + E Y Sbjct: 225 KYMMYVYDKWIFHIPLYFTVDYVSERLRSEVDFTTEANNSEHAREG------VEETDYLR 278 Query: 276 DKNEMPKTF 302 DK +PK + Sbjct: 279 DKIYIPKVY 287 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 25.8 bits (54), Expect = 5.7 Identities = 13/41 (31%), Positives = 17/41 (41%) Frame = +1 Query: 169 NKPSLKSVFYPYLTIPETGFQEIPSTLKYLSYNITLIRMKC 291 NK + FY L +P F P Y S T I ++C Sbjct: 120 NKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLEC 160 >SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |Schizosaccharomyces pombe|chr 3|||Manual Length = 690 Score = 25.8 bits (54), Expect = 5.7 Identities = 16/49 (32%), Positives = 28/49 (57%) Frame = +3 Query: 405 SGPDGKEIPPLSFVLTEFHTLLMYSDRVKVVSLLNKNLSTRIFTRKLMG 551 SG K IPP +LTE +L++ + ++S++ L T +F K++G Sbjct: 212 SGNSIKTIPPELGLLTELREVLLFDN---MISVIPAELGT-LFQLKILG 256 >SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharomyces pombe|chr 2|||Manual Length = 733 Score = 25.8 bits (54), Expect = 5.7 Identities = 16/51 (31%), Positives = 22/51 (43%), Gaps = 2/51 (3%) Frame = +1 Query: 313 PNRVSSMVSWTRHLNRTQILLSPKARS*PTLRDRTAK--KYPHYHLFSRNS 459 P R S SW+ L R + SPK +S R K+PH + N+ Sbjct: 242 PKRSPSKGSWSSILRRPSLTYSPKRQSTGLHRRSLTNYFKFPHRNAHQHNA 292 >SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces pombe|chr 1|||Manual Length = 684 Score = 25.8 bits (54), Expect = 5.7 Identities = 13/41 (31%), Positives = 21/41 (51%) Frame = -3 Query: 225 TCFWNG*IRIKD*LQRWFVLRKNGMPDELVNSCWCCHKYTV 103 +C W + +KD VLRK + ++N+ C KYT+ Sbjct: 472 SCVWTTEVPLKDAFDLKSVLRKTDNVESVLNA--CVEKYTL 510 >SPAC56F8.07 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 183 Score = 25.4 bits (53), Expect = 7.5 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = +1 Query: 157 IFSENKPSLKSVFYPYLTIP 216 +F+E K + S ++PYL IP Sbjct: 133 LFAEKKWMIMSFYFPYLAIP 152 >SPCC61.05 |||S. pombe specific multicopy membrane protein family 1|Schizosaccharomyces pombe|chr 3|||Manual Length = 469 Score = 25.4 bits (53), Expect = 7.5 Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 4/60 (6%) Frame = +1 Query: 175 PSLKSVFYPYLTIPETGFQEIPSTLKYLSYNITLIR----MKCLKRLPGLPNRVSSMVSW 342 P L FY PE+ F+ I STL +S + I ++C+K G+ S+ W Sbjct: 179 PVLTVEFYGDAPPPESYFRTIKSTLLAISMFLGFITLTWLLRCIKSQSGVQPAQISLAFW 238 >SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 507 Score = 25.4 bits (53), Expect = 7.5 Identities = 14/42 (33%), Positives = 21/42 (50%) Frame = -2 Query: 610 FFRKHPNLSFLQVAYYCFEFPMSFRVNILVDKFLLSRDTTLT 485 FF K LSF ++ F F +F ++ D + S TT+T Sbjct: 62 FFIKMRYLSFFFEFFFLFSFAFAFDFDVTSDDSINSALTTVT 103 >SPCC1919.12c |||aminopeptidase |Schizosaccharomyces pombe|chr 3|||Manual Length = 843 Score = 25.0 bits (52), Expect = 9.9 Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 2/44 (4%) Frame = -2 Query: 574 VAYYCFE--FPMSFRVNILVDKFLLSRDTTLTRSEYISRVWNSV 449 V Y+ F FP+ +ILV+ L + TLT S + S+++ V Sbjct: 570 VIYFIFNVPFPVFLCYDILVETILPAGSQTLTDSVFSSKLYKLV 613 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 25.0 bits (52), Expect = 9.9 Identities = 7/28 (25%), Positives = 20/28 (71%) Frame = +1 Query: 253 YLSYNITLIRMKCLKRLPGLPNRVSSMV 336 + + ++ ++RM+ + ++P LP+R+ M+ Sbjct: 1208 HAAMDVYVLRMETITKIPNLPSRIKFML 1235 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.0 bits (52), Expect = 9.9 Identities = 8/16 (50%), Positives = 14/16 (87%) Frame = +2 Query: 623 YKIVREERNLWQIYSD 670 Y+IV ++R++W+ YSD Sbjct: 43 YEIVHQKRSVWRRYSD 58 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,098,649 Number of Sequences: 5004 Number of extensions: 69892 Number of successful extensions: 214 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 204 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 214 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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