Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20372
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC962.03c |cut15||karyopherin Cut15|Schizosaccharomyces pombe|... 74 2e-14
SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr... 73 4e-14
SPBC1773.01 |||striatin homolog|Schizosaccharomyces pombe|chr 2|... 30 0.37
SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 27 2.0
SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom... 27 2.0
SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces po... 27 3.4
SPAC1F7.02c |||ATP-dependent RNA helicase Has1 |Schizosaccharomy... 27 3.4
SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr ... 27 3.4
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 26 4.5
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 26 6.0
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 26 6.0
SPAP27G11.15 |slx1||structure-specific endonuclease catalytic su... 26 6.0
SPCC1827.04 |||ankyrin repeat protein, unknown biological role|S... 25 7.9
SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyce... 25 7.9
>SPCC962.03c |cut15||karyopherin Cut15|Schizosaccharomyces pombe|chr
3|||Manual
Length = 542
Score = 73.7 bits (173), Expect = 2e-14
Identities = 37/69 (53%), Positives = 50/69 (72%)
Frame = +1
Query: 493 ATMQKMLSCDKNPPIDELIAAGILPILVQCLSRADNPALQFETAWALTNIASGTSAQTNK 672
A +K LS + NPPI ++I AG++P V+ LS +N L+FE +WALTN+ASG+S QT+
Sbjct: 100 AKFRKALSKETNPPIQKVIDAGVVPRFVEFLSHENN-LLKFEASWALTNVASGSSNQTHV 158
Query: 673 VVHAGAVPV 699
VV A AVPV
Sbjct: 159 VVEANAVPV 167
Score = 46.8 bits (106), Expect = 3e-06
Identities = 34/89 (38%), Positives = 48/89 (53%), Gaps = 7/89 (7%)
Frame = +2
Query: 263 FKNAGK-DVDEMRRRRNEVTVELRKNKREETLQKRRNVPISYSTDEEEID-KNLATTDLD 436
+K G DE+RRRR +E+RK KREE L KRRN+ EE I + DL+
Sbjct: 16 YKGKGTFQADELRRRRETQQIEIRKQKREENLNKRRNLVDVQEPAEETIPLEQDKENDLE 75
Query: 437 -EL----VMNAANAENPEAQLAAVQQCRK 508
EL ++ A +++ EAQ+ A + RK
Sbjct: 76 LELQLPDLLKALYSDDIEAQIQATAKFRK 104
>SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr
2|||Manual
Length = 539
Score = 72.9 bits (171), Expect = 4e-14
Identities = 37/72 (51%), Positives = 50/72 (69%)
Frame = +1
Query: 481 VGCCATMQKMLSCDKNPPIDELIAAGILPILVQCLSRADNPALQFETAWALTNIASGTSA 660
+G +K LS + +PPID++IA G++ VQ L +++ LQFE AWALTNIASGT+
Sbjct: 95 LGAVTKFRKYLSKETHPPIDQVIACGVVDRFVQFLE-SEHHLLQFEAAWALTNIASGTTD 153
Query: 661 QTNKVVHAGAVP 696
QT VV +GAVP
Sbjct: 154 QTRIVVDSGAVP 165
Score = 50.8 bits (116), Expect = 2e-07
Identities = 36/91 (39%), Positives = 51/91 (56%), Gaps = 9/91 (9%)
Frame = +2
Query: 263 FKNAGK-DVDEMRRRRNEVTVELRKNKREETLQKRRNVPISYSTD---EEEIDKNLATTD 430
FK+ G DE+RR+R E +E+RK KREE+L KRRN+ D EEE D++ +
Sbjct: 13 FKSKGVFKADELRRQREEQQIEIRKQKREESLNKRRNLNAVLQNDIDVEEEADQSQVQME 72
Query: 431 ---LDELVMNAAN--AENPEAQLAAVQQCRK 508
DE A+ +++ E QL AV + RK
Sbjct: 73 QQMKDEFPKLTADVMSDDIELQLGAVTKFRK 103
Score = 32.7 bits (71), Expect = 0.052
Identities = 20/61 (32%), Positives = 32/61 (52%)
Frame = +1
Query: 511 LSCDKNPPIDELIAAGILPILVQCLSRADNPALQFETAWALTNIASGTSAQTNKVVHAGA 690
LS N I ++ G P LV+ LS + + +Q ++ NI +GT AQT ++ GA
Sbjct: 275 LSDGPNEKIGAILDVGCAPRLVELLS-SPSVNIQTPALRSVGNIVTGTDAQTQIIIDCGA 333
Query: 691 V 693
+
Sbjct: 334 L 334
Score = 28.3 bits (60), Expect = 1.1
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +1
Query: 544 LIAAGILPILVQCLSRADNPALQFETAWALTNIASGTSAQTNKVVHAGAV 693
++ +G +P +Q LS + + + WAL NIA +SA + V+ G +
Sbjct: 158 VVDSGAVPRFIQLLSSPEKDVRE-QVVWALGNIAGDSSACRDYVLGNGVL 206
Score = 26.2 bits (55), Expect = 4.5
Identities = 15/55 (27%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +1
Query: 538 DELIAAGILPILVQCL-SRADNPALQFETAWALTNIASGTSAQTNKVVHAGAVPV 699
D ++ G+L L+ L S A + ++ W L+N+ G + N + AVP+
Sbjct: 198 DYVLGNGVLQPLLNILQSSASDVSMLRNATWTLSNLCRGKNPPPNWSTISVAVPI 252
>SPBC1773.01 |||striatin homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 612
Score = 29.9 bits (64), Expect = 0.37
Identities = 20/68 (29%), Positives = 35/68 (51%), Gaps = 1/68 (1%)
Frame = +2
Query: 290 EMRRRRNEVTVELRKNKREETLQKRRNVPISYSTDEEEIDKNLATTDLDEL-VMNAANAE 466
E++ R ++ E K ++ + Q+RR + S E DKN++ DLDE +++ A
Sbjct: 51 ELKIRVAQLERERAKLEQSLSFQQRRAEMLEKSLRELRKDKNISVKDLDEFHLLDKPAAN 110
Query: 467 NPEAQLAA 490
N +A A
Sbjct: 111 NTKADAEA 118
>SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 376
Score = 27.5 bits (58), Expect = 2.0
Identities = 17/60 (28%), Positives = 31/60 (51%)
Frame = +2
Query: 188 NYHVLCRDSEHR*NGD*SSEKPLHVFKNAGKDVDEMRRRRNEVTVELRKNKREETLQKRR 367
N+ RDS+ E L ++ K++DE RR++ E+ +E ++ E+ L+K R
Sbjct: 275 NHQFHSRDSQPITQRHTDIESRLQKMQDNAKELDESRRKKIEL-LEKKERDEEQFLEKER 333
>SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 649
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = +2
Query: 305 RNEVTVELRKNKREETLQKRRNVPISYSTDEEEIDKNLATT 427
R + ++ ++N+ T+QK V S+ EEE K+L T
Sbjct: 302 RKKKSIPKKQNESSSTIQKENTVQQEASSSEEEAVKSLPET 342
>SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 942
Score = 26.6 bits (56), Expect = 3.4
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = -3
Query: 608 NAGLSALERH*TRMGSIPAAMSSSIGGFLSHESI 507
N+ A R+ G P A+ + GFL H+SI
Sbjct: 599 NSATRAAVRYCLNRGHTPLAIDNGFSGFLRHDSI 632
>SPAC1F7.02c |||ATP-dependent RNA helicase Has1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 578
Score = 26.6 bits (56), Expect = 3.4
Identities = 11/50 (22%), Positives = 27/50 (54%)
Frame = +2
Query: 290 EMRRRRNEVTVELRKNKREETLQKRRNVPISYSTDEEEIDKNLATTDLDE 439
E++R++++ E K KR++ L+ + + DE++ ++ D D+
Sbjct: 5 ELKRKKHQSGNEEVKEKRQKPLKNDKKIAEELPQDEDDYEQEEENEDADQ 54
>SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 513
Score = 26.6 bits (56), Expect = 3.4
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -1
Query: 466 FCVGSVHHQLIKVGGSEVLVDLLLI 392
+C G H ++ +GGS V +DL+ I
Sbjct: 263 YCTGDGHKMVMSIGGSTVDLDLVQI 287
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/34 (38%), Positives = 25/34 (73%)
Frame = -2
Query: 492 TAANCASGFSALAAFITSSSRSVVARFLSISSSS 391
++++ S FS++++ ++SS+ S + LSISSSS
Sbjct: 565 SSSSIPSTFSSVSSILSSSTSSPSSTSLSISSSS 598
>SPBC3E7.01 |fab1|ste12,
SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1932
Score = 25.8 bits (54), Expect = 6.0
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = -3
Query: 251 VFRLISRHFICARCLDTVRGNYTKKQRINIKLLSSK 144
+FRL +C C + NY++ Q N L SK
Sbjct: 101 IFRLQGSIKVCRPCASILVNNYSRSQLFNHSLNESK 136
>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2176
Score = 25.8 bits (54), Expect = 6.0
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = -1
Query: 700 IQVLHRHGLLCLFVQTFRKLCLLKPKRSRTVTLDCQPSKGTE 575
+Q L+RHGL+ + + + + LK K + + + Q G E
Sbjct: 1667 VQYLYRHGLIKVLIASRDVIYSLKAKSNAVIVMGTQYYDGKE 1708
>SPAP27G11.15 |slx1||structure-specific endonuclease catalytic
subunit |Schizosaccharomyces pombe|chr 1|||Manual
Length = 271
Score = 25.8 bits (54), Expect = 6.0
Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Frame = +2
Query: 392 DEEEIDKNLATTD---LDELVMNAANAENPEAQLAAVQ-QCRKCSRVIKTRLLMSS 547
+ +E+ N TD ++ L A++ E Q+ ++ C KC RV++ R +S+
Sbjct: 188 ESDELRANCPFTDCNSINHLTCLASSFLTEECQVLPIEGMCTKCKRVLRWREFLST 243
>SPCC1827.04 |||ankyrin repeat protein, unknown biological
role|Schizosaccharomyces pombe|chr 3|||Manual
Length = 600
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/39 (28%), Positives = 25/39 (64%)
Frame = +2
Query: 257 HVFKNAGKDVDEMRRRRNEVTVELRKNKREETLQKRRNV 373
HV + K +E R+R+ E+ E+R+++ +E +K++ +
Sbjct: 352 HVDSISIKAQEEERKRQAEIEKEIRQSRLQEEERKKKKL 390
>SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 351
Score = 25.4 bits (53), Expect = 7.9
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +2
Query: 317 TVELRKNKREETLQKRRNVPISYSTDEEEIDKNL 418
T+E KN +T + ++ +SY D + +DK +
Sbjct: 137 TLETEKNLNSKTSESLKSPTLSYPFDLDSLDKRI 170
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,922,399
Number of Sequences: 5004
Number of extensions: 61922
Number of successful extensions: 239
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 237
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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