Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20371
(544 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 159 2e-40
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 155 3e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 89 5e-19
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 64 2e-11
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.016
SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 25 5.5
SPBC1271.09 |||glycerophosphodiester transporter|Schizosaccharom... 25 7.2
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 25 9.5
SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyc... 25 9.5
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 159 bits (387), Expect = 2e-40
Identities = 73/85 (85%), Positives = 78/85 (91%)
Frame = +3
Query: 255 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 434
VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITA
Sbjct: 185 VVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITA 244
Query: 435 SLRFDGALNVDLTEFQTNLVPYPRI 509
SLRF G+LNVDL EFQTNLVPYPRI
Sbjct: 245 SLRFAGSLNVDLNEFQTNLVPYPRI 269
Score = 116 bits (279), Expect = 2e-27
Identities = 48/82 (58%), Positives = 66/82 (80%)
Frame = +1
Query: 1 RHEAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLME 180
+ +A+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH LL+E
Sbjct: 100 KEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLE 159
Query: 181 RLSVDYGKKSKLEFAIYPAPQV 246
RL+++YGKKS L+F++YPAPQV
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQV 181
Score = 29.5 bits (63), Expect = 0.34
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +2
Query: 500 PPYHFPLVTYAPVIS 544
P HFPLVTY+P++S
Sbjct: 267 PRIHFPLVTYSPIVS 281
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 155 bits (377), Expect = 3e-39
Identities = 70/85 (82%), Positives = 78/85 (91%)
Frame = +3
Query: 255 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 434
VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITA
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240
Query: 435 SLRFDGALNVDLTEFQTNLVPYPRI 509
SLRF+G+LNVDL EFQTNLVPYPRI
Sbjct: 241 SLRFEGSLNVDLAEFQTNLVPYPRI 265
Score = 113 bits (273), Expect = 1e-26
Identities = 48/82 (58%), Positives = 65/82 (79%)
Frame = +1
Query: 1 RHEAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLME 180
+ +A+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH LL+E
Sbjct: 96 KEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLE 155
Query: 181 RLSVDYGKKSKLEFAIYPAPQV 246
RL+++Y KKSKL+F++YPAPQV
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQV 177
Score = 30.7 bits (66), Expect = 0.15
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = +2
Query: 500 PPYHFPLVTYAPVIS 544
P HFPLVTYAP++S
Sbjct: 263 PRIHFPLVTYAPIVS 277
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 88.6 bits (210), Expect = 5e-19
Identities = 39/93 (41%), Positives = 57/93 (61%)
Frame = +3
Query: 231 PRASGFHCVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIG 410
P VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVS 230
Query: 411 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRI 509
+++ +T S RF G LN DL + N+VP+PR+
Sbjct: 231 AVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRL 263
Score = 61.7 bits (143), Expect = 7e-11
Identities = 28/78 (35%), Positives = 43/78 (55%)
Frame = +1
Query: 10 AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 189
A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H LL+ ++
Sbjct: 97 AGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIR 156
Query: 190 VDYGKKSKLEFAIYPAPQ 243
+Y + F++ PAP+
Sbjct: 157 EEYPDRMMATFSVAPAPK 174
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 63.7 bits (148), Expect = 2e-11
Identities = 29/84 (34%), Positives = 48/84 (57%)
Frame = +3
Query: 255 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 434
VV+PYNS+L ++D ++DN A+ I L + PT+ N+L+ ++S+ T
Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTT 241
Query: 435 SLRFDGALNVDLTEFQTNLVPYPR 506
+LR+ G +N DL +L+P PR
Sbjct: 242 TLRYPGYMNNDLVSIIASLIPSPR 265
Score = 43.2 bits (97), Expect = 3e-05
Identities = 24/78 (30%), Positives = 39/78 (50%)
Frame = +1
Query: 10 AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 189
A NN+A G Y+ + I + ++D I + AD L+GF + H L+ERL+
Sbjct: 100 AGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLN 158
Query: 190 VDYGKKSKLEFAIYPAPQ 243
Y KK ++++P Q
Sbjct: 159 DRYPKKIIQTYSVFPNSQ 176
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 33.9 bits (74), Expect = 0.016
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = -3
Query: 497 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 336
R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPAC6G9.04 |mug79||meiotically upregulated gene
Mug79|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 25.4 bits (53), Expect = 5.5
Identities = 15/58 (25%), Positives = 27/58 (46%)
Frame = -3
Query: 491 QVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDGFIVDHE 318
Q ++ VH ++A E R N + QVG +N ++ D+ + F+ D+E
Sbjct: 601 QSQIKSQSVHHENAAEEIRKMRNLRNSRINSQQVGEKVFVNPDVKTMDIQETFLQDYE 658
>SPBC1271.09 |||glycerophosphodiester
transporter|Schizosaccharomyces pombe|chr 2|||Manual
Length = 543
Score = 25.0 bits (52), Expect = 7.2
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = -2
Query: 93 SELADSVQNQIYDFLSNGVVTTGIVVGRLVP 1
+EL +++ + +++G + G VVG LVP
Sbjct: 206 NELKSGLRHAAFIVVTDGAIDFGFVVGALVP 236
>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 649
Score = 24.6 bits (51), Expect = 9.5
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +1
Query: 1 RHEAANNYARGHYTIGKEIVDLV 69
R ANN+ RGH I K+ D +
Sbjct: 599 RERKANNFFRGHIEIDKKTKDFL 621
>SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 313
Score = 24.6 bits (51), Expect = 9.5
Identities = 14/41 (34%), Positives = 20/41 (48%), Gaps = 3/41 (7%)
Frame = -2
Query: 138 EGVEDQESLQTGTLVSELA---DSVQNQIYDFLSNGVVTTG 25
E +ED+E S L D + + YD ++NGV TG
Sbjct: 147 EVIEDEEKRVVDLYPSSLRTKIDELNDYFYDTVNNGVYKTG 187
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,114,408
Number of Sequences: 5004
Number of extensions: 39358
Number of successful extensions: 152
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 223909422
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -