Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20367
(802 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 132 4e-32
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 125 6e-30
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 124 2e-29
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 102 5e-23
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 95 1e-20
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 28 1.3
SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 27 2.3
SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces... 26 5.4
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 7.2
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 132 bits (320), Expect = 4e-32
Identities = 64/81 (79%), Positives = 71/81 (87%), Gaps = 1/81 (1%)
Frame = +3
Query: 12 KSQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVTFEIDAN 191
KSQIFSTA DNQ+TV IQVYEGER +TKDN+LLGKFDL GIPPAPRG+PQIEVTFE+DAN
Sbjct: 451 KSQIFSTAVDNQNTVLIQVYEGERTLTKDNNLLGKFDLRGIPPAPRGVPQIEVTFEVDAN 510
Query: 192 GILQVSAEDK-GTGNREKIVI 251
G+L VSA DK G G EK+VI
Sbjct: 511 GVLTVSAVDKSGKGKPEKLVI 531
Score = 72.1 bits (169), Expect = 8e-14
Identities = 36/90 (40%), Positives = 50/90 (55%)
Frame = +2
Query: 221 GNRKQRKNCNHYDQNRLTPEDIERMIXXXXXXXXXXXXXXXRVESRNELESYAYSIKNQL 400
G K K D+ RL+ EDIERM+ R+E+RN LE+YAYS+K Q
Sbjct: 522 GKGKPEKLVIKNDKGRLSEEDIERMVKEAEEFAEEDKILKERIEARNTLENYAYSLKGQF 581
Query: 401 QDKEKLGAKVTDDDKAKMEEALDAAIKWLE 490
D E+LG KV +DK + +A++ +WLE
Sbjct: 582 DDDEQLGGKVDPEDKQAVLDAVEDVAEWLE 611
Score = 27.1 bits (57), Expect = 3.1
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +1
Query: 511 EEYKKQKKTLEDVVQPIIAKLY 576
EE++ Q++ L+ VV PI KLY
Sbjct: 620 EEFEDQRQKLDAVVHPITQKLY 641
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 125 bits (302), Expect = 6e-30
Identities = 59/82 (71%), Positives = 70/82 (85%)
Frame = +3
Query: 9 EKSQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVTFEIDA 188
+KS+IFST SDNQ V IQV+EGER TKD +LLGKF+L+GIPPAPRG+PQIEVTF++DA
Sbjct: 421 KKSEIFSTYSDNQPGVLIQVFEGERARTKDCNLLGKFELSGIPPAPRGVPQIEVTFDVDA 480
Query: 189 NGILQVSAEDKGTGNREKIVIT 254
NGIL VSA +KGTG +KI IT
Sbjct: 481 NGILNVSALEKGTGKTQKITIT 502
Score = 62.1 bits (144), Expect = 9e-11
Identities = 33/95 (34%), Positives = 51/95 (53%)
Frame = +2
Query: 221 GNRKQRKNCNHYDQNRLTPEDIERMIXXXXXXXXXXXXXXXRVESRNELESYAYSIKNQL 400
G K +K D+ RL+ E+I+RM+ R++++N LESYAYS++N L
Sbjct: 492 GTGKTQKITITNDKGRLSKEEIDRMVAEAEKYKAEDEAESGRIQAKNHLESYAYSLRNSL 551
Query: 401 QDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDA 505
D L KV DK +++A+ I+WL+ N A
Sbjct: 552 DD-PNLKDKVDASDKETVDKAVKETIEWLDSNTTA 585
Score = 27.9 bits (59), Expect = 1.8
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = +1
Query: 511 EEYKKQKKTLEDVVQPIIAKLY 576
+E++ ++K LE V PI+AK+Y
Sbjct: 588 DEFEAKQKELESVANPIMAKIY 609
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 124 bits (298), Expect = 2e-29
Identities = 57/82 (69%), Positives = 70/82 (85%)
Frame = +3
Query: 9 EKSQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVTFEIDA 188
+KS++FST +DNQ V IQV+EGER TKD +LLGKF+L+GIPPAPRG+PQIEVTF++DA
Sbjct: 421 KKSEVFSTYADNQPGVLIQVFEGERARTKDCNLLGKFELSGIPPAPRGVPQIEVTFDVDA 480
Query: 189 NGILQVSAEDKGTGNREKIVIT 254
NGIL VSA +KGTG +KI IT
Sbjct: 481 NGILNVSALEKGTGKTQKITIT 502
Score = 61.7 bits (143), Expect = 1e-10
Identities = 33/95 (34%), Positives = 51/95 (53%)
Frame = +2
Query: 221 GNRKQRKNCNHYDQNRLTPEDIERMIXXXXXXXXXXXXXXXRVESRNELESYAYSIKNQL 400
G K +K D+ RL+ E+I+RM+ R++++N LESYAYS++N L
Sbjct: 492 GTGKTQKITITNDKGRLSKEEIDRMVSEAEKYKAEDEAETSRIQAKNHLESYAYSLRNSL 551
Query: 401 QDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDA 505
D L KV DK +++A+ I+WL+ N A
Sbjct: 552 DD-PNLKDKVDASDKEAIDKAVKETIEWLDHNTTA 585
Score = 29.1 bits (62), Expect = 0.77
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +1
Query: 511 EEYKKQKKTLEDVVQPIIAKLY 576
+EY+ ++K LE V PI+AK+Y
Sbjct: 588 DEYEDKQKELEGVANPIMAKIY 609
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 102 bits (245), Expect = 5e-23
Identities = 45/80 (56%), Positives = 60/80 (75%)
Frame = +3
Query: 12 KSQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVTFEIDAN 191
KSQ+FSTA+D Q V I+V++GER + +DN L+G F LTGI PAP+G PQIEV+F++DA+
Sbjct: 465 KSQVFSTAADGQTAVEIRVFQGERELVRDNKLIGNFQLTGIAPAPKGQPQIEVSFDVDAD 524
Query: 192 GILQVSAEDKGTGNREKIVI 251
GI+ VSA DK T I +
Sbjct: 525 GIINVSARDKATNKDSSITV 544
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 95.1 bits (226), Expect = 1e-20
Identities = 45/81 (55%), Positives = 57/81 (70%)
Frame = +3
Query: 12 KSQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVTFEIDAN 191
K + F+T +DNQ TVT VY+GER +N LG+F LTGIPP PRG ++E TFE+DAN
Sbjct: 427 KKRTFTTVADNQTTVTFPVYQGERVNCAENEPLGEFQLTGIPPMPRGQAELEATFELDAN 486
Query: 192 GILQVSAEDKGTGNREKIVIT 254
GIL+V+A +K TG I IT
Sbjct: 487 GILKVTAVEKTTGRSAHIEIT 507
>SPAPJ696.01c |vps17||retromer complex subunit
Vps17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 549
Score = 28.3 bits (60), Expect = 1.3
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +2
Query: 347 VESRNELESYAYSIKNQLQDKEKLGAKVTDDDKAKMEEA 463
+ S+N+ SY S N+LQD K+ TDD +E A
Sbjct: 355 ISSKNQTNSYL-SAANRLQDSPKISKARTDDALQALEVA 392
>SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1147
Score = 27.5 bits (58), Expect = 2.3
Identities = 13/30 (43%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Frame = +2
Query: 386 IKNQLQD--KEKLGAKVTDDDKAKMEEALD 469
+KNQ+ D KEKL +DD+K ++E D
Sbjct: 1094 LKNQIDDLAKEKLPLSSSDDEKVNIKEKTD 1123
>SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 796
Score = 26.2 bits (55), Expect = 5.4
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -3
Query: 236 SVSCSLIFSRYLQNTVGINFECDFNLWNT 150
S SC IF +L VG +F C ++ W+T
Sbjct: 427 SYSCDKIFGGFLLGVVGSDFICFYD-WDT 454
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.8 bits (54), Expect = 7.2
Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 4/38 (10%)
Frame = -1
Query: 229 PVPLSSADTCKIPLAS----ISNVTSICGIPRGAGGIP 128
PVPL SAD IP+ S + TS +P+ + G P
Sbjct: 1023 PVPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAP 1060
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,864,184
Number of Sequences: 5004
Number of extensions: 51840
Number of successful extensions: 169
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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