BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20367 (802 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 154 7e-38 SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 126 3e-29 SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 125 5e-29 SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 122 3e-28 SB_38725| Best HMM Match : No HMM Matches (HMM E-Value=.) 120 2e-27 SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 51 1e-06 SB_40385| Best HMM Match : Kelch_1 (HMM E-Value=0) 34 0.12 SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.27 SB_34832| Best HMM Match : SAP (HMM E-Value=1.4e-07) 30 2.5 SB_35649| Best HMM Match : M (HMM E-Value=6e-09) 29 3.3 SB_57638| Best HMM Match : F5_F8_type_C (HMM E-Value=1.7e-11) 29 3.3 >SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) Length = 1327 Score = 154 bits (374), Expect = 7e-38 Identities = 70/82 (85%), Positives = 78/82 (95%) Frame = +3 Query: 9 EKSQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVTFEIDA 188 +KSQ+FSTA+DNQ+TVTIQV+EGERPMTKDNH LGKFDL GIPPAPRG+PQIEVTFEID Sbjct: 1118 KKSQVFSTAADNQNTVTIQVFEGERPMTKDNHPLGKFDLNGIPPAPRGVPQIEVTFEIDV 1177 Query: 189 NGILQVSAEDKGTGNREKIVIT 254 NGIL+VSAEDKGTGN+EKI IT Sbjct: 1178 NGILRVSAEDKGTGNKEKITIT 1199 Score = 106 bits (254), Expect = 2e-23 Identities = 54/113 (47%), Positives = 73/113 (64%) Frame = +2 Query: 221 GNRKQRKNCNHYDQNRLTPEDIERMIXXXXXXXXXXXXXXXRVESRNELESYAYSIKNQL 400 GN+++ N DQNRLTPEDIERM+ +VE+RNELESYAYS+KNQ+ Sbjct: 1191 GNKEKITITN--DQNRLTPEDIERMVNDAEKFADEDKKTKEKVEARNELESYAYSLKNQV 1248 Query: 401 QDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAELKNTXXXXXXLKM*CSP 559 DKEKLG K+++DDK + EA++ AI W++ NQDA +++ KM SP Sbjct: 1249 GDKEKLGGKLSEDDKKTITEAVEKAISWMDKNQDASVEDFKKEKRKWKMLYSP 1301 >SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) Length = 640 Score = 126 bits (303), Expect = 3e-29 Identities = 58/80 (72%), Positives = 68/80 (85%) Frame = +3 Query: 15 SQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVTFEIDANG 194 +Q F+T SDNQ VTI+V+EGERP+TK N+LLGKFDL+GIPPAPRG+PQIEVTF+IDANG Sbjct: 426 TQTFTTYSDNQPAVTIRVFEGERPLTKHNNLLGKFDLSGIPPAPRGVPQIEVTFDIDANG 485 Query: 195 ILQVSAEDKGTGNREKIVIT 254 IL VSA+DK TG I IT Sbjct: 486 ILNVSAKDKSTGKTGSITIT 505 Score = 58.0 bits (134), Expect = 8e-09 Identities = 30/101 (29%), Positives = 51/101 (50%) Frame = +2 Query: 257 DQNRLTPEDIERMIXXXXXXXXXXXXXXXRVESRNELESYAYSIKNQLQDKEKLGAKVTD 436 D+ RL+ E+I+RMI ++ +RN LESYA+ +K+ + + L K++ Sbjct: 507 DKGRLSKEEIDRMINDAEKYKSEDEAQREKIAARNRLESYAFGVKSAISE-PSLEGKLSQ 565 Query: 437 DDKAKMEEALDAAIKWLEDNQDAELKNTXXXXXXLKM*CSP 559 DK ++ ++ + WLE N AE + L+ CSP Sbjct: 566 SDKDTVKNKVEEVLNWLEKNSLAEKEEFEEQEKELQRVCSP 606 >SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 746 Score = 125 bits (301), Expect = 5e-29 Identities = 56/82 (68%), Positives = 70/82 (85%) Frame = +3 Query: 9 EKSQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVTFEIDA 188 +++Q F+T SDNQ +V IQVYEGER +TKDN+LLGKF+L+GIPPAPRG+PQI+VTF++D+ Sbjct: 517 KQTQTFTTYSDNQTSVLIQVYEGERSLTKDNNLLGKFELSGIPPAPRGVPQIDVTFDVDS 576 Query: 189 NGILQVSAEDKGTGNREKIVIT 254 NGIL VSA DK TG KI IT Sbjct: 577 NGILNVSAVDKSTGKENKITIT 598 Score = 72.1 bits (169), Expect = 5e-13 Identities = 38/110 (34%), Positives = 61/110 (55%) Frame = +2 Query: 230 KQRKNCNHYDQNRLTPEDIERMIXXXXXXXXXXXXXXXRVESRNELESYAYSIKNQLQDK 409 K+ K D+ RL+ EDIERM+ R++S+N LESYA+S+K+ ++D Sbjct: 591 KENKITITNDKGRLSKEDIERMVKEAEKFKAADEAVRERIQSKNSLESYAFSMKSTMED- 649 Query: 410 EKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAELKNTXXXXXXLKM*CSP 559 EK+ K+++D++ K+ A + WLE NQ AE + L+ C+P Sbjct: 650 EKVKDKLSEDEREKVISRCKATLDWLEHNQSAEKEEIDAHQKELEGVCNP 699 >SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) Length = 1097 Score = 122 bits (295), Expect = 3e-28 Identities = 58/82 (70%), Positives = 67/82 (81%) Frame = +3 Query: 9 EKSQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVTFEIDA 188 +++Q F+T SDNQ V IQV+EGER MT N+LLGKF+LTGIPPAPRG+PQIEVTF+IDA Sbjct: 425 KQTQTFTTYSDNQPGVLIQVFEGERSMTAHNNLLGKFELTGIPPAPRGVPQIEVTFDIDA 484 Query: 189 NGILQVSAEDKGTGNREKIVIT 254 NGIL VSA DK TG KI IT Sbjct: 485 NGILNVSAVDKSTGKENKITIT 506 Score = 69.7 bits (163), Expect = 3e-12 Identities = 36/110 (32%), Positives = 60/110 (54%) Frame = +2 Query: 230 KQRKNCNHYDQNRLTPEDIERMIXXXXXXXXXXXXXXXRVESRNELESYAYSIKNQLQDK 409 K+ K D+ RL+ EDIERM+ R++++N LESYAYS+K+ ++D Sbjct: 499 KENKITITNDKGRLSKEDIERMVNEASKYKEEDEKQRDRIQTKNSLESYAYSMKSTVED- 557 Query: 410 EKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAELKNTXXXXXXLKM*CSP 559 +K+ K++++DK + + +KWL+ NQ AE L+ C+P Sbjct: 558 DKVKDKISEEDKKAILDKCTEVLKWLDTNQTAEKDEFEYHQKELEKVCNP 607 >SB_38725| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 766 Score = 120 bits (288), Expect = 2e-27 Identities = 54/81 (66%), Positives = 67/81 (82%) Frame = +3 Query: 9 EKSQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVTFEIDA 188 +KSQ+FSTA+D Q V I+V++GER M DN LLG+F L GIPPAPRG+PQ+EVTF+IDA Sbjct: 229 KKSQVFSTAADGQTQVEIKVFQGEREMAIDNKLLGQFQLVGIPPAPRGVPQVEVTFDIDA 288 Query: 189 NGILQVSAEDKGTGNREKIVI 251 NGI+ VSA DKGTG ++IVI Sbjct: 289 NGIVNVSARDKGTGREQQIVI 309 >SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) Length = 212 Score = 50.8 bits (116), Expect = 1e-06 Identities = 23/39 (58%), Positives = 30/39 (76%) Frame = +3 Query: 9 EKSQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDL 125 +KSQ+FSTA+D Q V I+V++GER M DN LLG+F L Sbjct: 174 KKSQVFSTAADGQTQVEIKVFQGEREMAIDNKLLGQFQL 212 >SB_40385| Best HMM Match : Kelch_1 (HMM E-Value=0) Length = 823 Score = 34.3 bits (75), Expect = 0.12 Identities = 16/53 (30%), Positives = 27/53 (50%) Frame = +2 Query: 344 RVESRNELESYAYSIKNQLQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQD 502 + E++ EL++ S K + D +K + T DD+ D +WLE+N D Sbjct: 732 KAEAQFELKALITSTKETIADPDKHMGRFTKDDRVSGNRYCDEKSEWLENNAD 784 >SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1019 Score = 33.1 bits (72), Expect = 0.27 Identities = 15/49 (30%), Positives = 33/49 (67%), Gaps = 1/49 (2%) Frame = +2 Query: 353 SRNELESYAYSIKNQLQDKEKLGAKV-TDDDKAKMEEALDAAIKWLEDN 496 ++N LES+ + +++++ + LG K+ T+ ++ + EAL AA WL+++ Sbjct: 719 AKNALESHIFGVRDEMNSE--LGEKLSTEAERETISEALTAASDWLDED 765 >SB_34832| Best HMM Match : SAP (HMM E-Value=1.4e-07) Length = 1054 Score = 29.9 bits (64), Expect = 2.5 Identities = 15/51 (29%), Positives = 31/51 (60%) Frame = +2 Query: 356 RNELESYAYSIKNQLQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAE 508 +N +E + + +NQ + ++K K TD+DKAK EE +++ ++++ E Sbjct: 928 QNIMELFEHDSENQKRSEQK--NKHTDEDKAKAEEMQKQSLETFKESRKRE 976 >SB_35649| Best HMM Match : M (HMM E-Value=6e-09) Length = 1279 Score = 29.5 bits (63), Expect = 3.3 Identities = 33/132 (25%), Positives = 62/132 (46%), Gaps = 6/132 (4%) Frame = +2 Query: 344 RVESRNELESYAYSIKNQLQDKEKLGAKVTDDDKA-----KMEEALDAAIKWLEDNQDAE 508 + E + + A NQLQ EKLGA + D DKA +++E L A ++ D A Sbjct: 365 KAEEEKKAKRDANDHVNQLQ--EKLGAAMRDLDKANDHVNQLKEKLGAVMR---DLDKAN 419 Query: 509 LKNTXXXXXXLKM*CSPSSPNFTKARVEYRPRG-RRKMTTSRTSCNQTESQPNVISAQMA 685 +N ++ + + +E + +G R++ R++ N+ E++ V+S ++ Sbjct: 420 ERNKELNESVIQTQSEKDTIKHSLHMMEEQYKGLRQQQEDHRSTVNELETKLEVLSKKL- 478 Query: 686 AIVLRGECPTHD 721 +V E TH+ Sbjct: 479 -LVANEEKSTHE 489 >SB_57638| Best HMM Match : F5_F8_type_C (HMM E-Value=1.7e-11) Length = 502 Score = 29.5 bits (63), Expect = 3.3 Identities = 14/40 (35%), Positives = 18/40 (45%) Frame = +3 Query: 102 HLLGKFDLTGIPPAPRGIPQIEVTFEIDANGILQVSAEDK 221 HLL G P +PR + Q VTF D + +DK Sbjct: 222 HLLSAVATQGSPHSPRWVTQYYVTFSYDKENWFEYKVKDK 261 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 22,082,785 Number of Sequences: 59808 Number of extensions: 429358 Number of successful extensions: 1228 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 1109 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1222 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2215746665 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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