Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20360
(688 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P46459 Cluster: Vesicle-fusing ATPase; n=64; Eumetazoa|... 82 1e-14
UniRef50_Q94392 Cluster: Vesicle-fusing ATPase; n=3; Caenorhabdi... 77 3e-13
UniRef50_Q4U0S6 Cluster: N-ethylmaleimide-sensitive factor b; n=... 75 1e-12
UniRef50_Q5C7K1 Cluster: SJCHGC05550 protein; n=1; Schistosoma j... 72 1e-11
UniRef50_Q9P7Q4 Cluster: Vesicular-fusion protein SEC18 homolog;... 66 8e-10
UniRef50_Q5KEU7 Cluster: Vesicular-fusion protein sec18, putativ... 56 8e-07
UniRef50_Q9P4C9 Cluster: Sec18; n=1; Pichia pastoris|Rep: Sec18 ... 51 2e-05
UniRef50_P18759 Cluster: Vesicular-fusion protein SEC18; n=5; Sa... 49 1e-04
UniRef50_Q677Q6 Cluster: Cell division protein 48; n=1; Lymphocy... 47 4e-04
UniRef50_P34732 Cluster: Vesicular-fusion protein SEC18; n=6; Sa... 47 4e-04
UniRef50_Q9M0Y8 Cluster: Vesicle-fusing ATPase; n=9; Viridiplant... 46 0.001
UniRef50_A6XPW2 Cluster: Putative uncharacterized protein; n=2; ... 44 0.003
UniRef50_Q5B8N2 Cluster: Putative uncharacterized protein; n=1; ... 42 0.014
UniRef50_Q6CBG2 Cluster: Yarrowia lipolytica chromosome C of str... 36 0.93
UniRef50_Q5XH72 Cluster: LOC495051 protein; n=5; Tetrapoda|Rep: ... 36 1.2
UniRef50_UPI0000ECB9E1 Cluster: Apoptosis inhibitor 5 (API-5).; ... 33 4.9
UniRef50_A4UHE0 Cluster: Putative uncharacterized protein; n=1; ... 33 4.9
>UniRef50_P46459 Cluster: Vesicle-fusing ATPase; n=64;
Eumetazoa|Rep: Vesicle-fusing ATPase - Homo sapiens
(Human)
Length = 744
Score = 81.8 bits (193), Expect = 1e-14
Identities = 36/84 (42%), Positives = 59/84 (70%), Gaps = 1/84 (1%)
Frame = +3
Query: 258 FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSA-ECLCSVTLEADFMMK 434
+ F+++ + V G++ FS PQRKWA LSIGQ I+V + A +C+ ++T+E DF+ K
Sbjct: 45 YTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQK 104
Query: 435 KTTSTDPYDSEQMARDFLIQFANQ 506
K+ ++PYD+++MA +F+ QF NQ
Sbjct: 105 KSIDSNPYDTDKMAAEFIQQFNNQ 128
Score = 44.8 bits (101), Expect = 0.002
Identities = 17/34 (50%), Positives = 27/34 (79%)
Frame = +1
Query: 154 CPSDELAITNCALIHQDDFPSDIKHIEVSTGPSH 255
CP+DEL++TNCA++++ DF S +H+ V T P+H
Sbjct: 11 CPTDELSLTNCAVVNEKDFQSG-QHVIVRTSPNH 43
>UniRef50_Q94392 Cluster: Vesicle-fusing ATPase; n=3;
Caenorhabditis|Rep: Vesicle-fusing ATPase -
Caenorhabditis elegans
Length = 824
Score = 77.4 bits (182), Expect = 3e-13
Identities = 35/84 (41%), Positives = 51/84 (60%)
Frame = +3
Query: 255 HFVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSAECLCSVTLEADFMMK 434
H++FSIR + G + F P RKWA LS+ Q + V PF +E + S+ L ADF K
Sbjct: 108 HYIFSIRNDGSIKPGEIAFGVPHRKWAALSLDQEVRVTPFTFQQSEYVGSMILTADFNAK 167
Query: 435 KTTSTDPYDSEQMARDFLIQFANQ 506
K +++P +++ MAR+F IQF Q
Sbjct: 168 KNVTSEPLNADLMAREFSIQFGGQ 191
Score = 44.8 bits (101), Expect = 0.002
Identities = 26/75 (34%), Positives = 43/75 (57%), Gaps = 14/75 (18%)
Frame = +2
Query: 506 SFTVGQQLAFLFQEKK-----VLSLIVKNLEAVDVQALAAGANAV---------PRRVRM 643
+F+ G Q+AF F++K+ LSL+VK++E D+ AA A+ P+++
Sbjct: 192 AFSKGMQMAFRFEDKEKNKTHTLSLVVKSIEGFDIGKAAAAASGASNTDSSATKPKQIEA 251
Query: 644 GRLLPDASVQFDKAE 688
G LLP++ + FDK E
Sbjct: 252 GELLPNSVIVFDKEE 266
Score = 34.3 bits (75), Expect = 2.8
Identities = 14/34 (41%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Frame = +1
Query: 157 PSDELAITNCALIHQDDFPS-DIKHIEVSTGPSH 255
PS+E + N A +++ DF IKH+ V+ GP+H
Sbjct: 74 PSEEHTLANYAYVNRSDFDDKQIKHVRVNPGPAH 107
>UniRef50_Q4U0S6 Cluster: N-ethylmaleimide-sensitive factor b; n=8;
Clupeocephala|Rep: N-ethylmaleimide-sensitive factor b -
Danio rerio (Zebrafish) (Brachydanio rerio)
Length = 747
Score = 75.4 bits (177), Expect = 1e-12
Identities = 35/84 (41%), Positives = 56/84 (66%), Gaps = 1/84 (1%)
Frame = +3
Query: 258 FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFK-PSSAECLCSVTLEADFMMK 434
FVF++R + V GT+ FS PQRKWA LS+ Q ++V + S + + ++T+E DF+ K
Sbjct: 45 FVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQK 104
Query: 435 KTTSTDPYDSEQMARDFLIQFANQ 506
K+ ++PYDS++MA +F+ F Q
Sbjct: 105 KSIDSNPYDSDKMAIEFIQCFTAQ 128
Score = 37.1 bits (82), Expect = 0.40
Identities = 14/33 (42%), Positives = 23/33 (69%)
Frame = +1
Query: 154 CPSDELAITNCALIHQDDFPSDIKHIEVSTGPS 252
CP+DEL++TNC + + DF S +H+ + T P+
Sbjct: 11 CPTDELSLTNCVVASEKDFKSG-QHLTIKTTPT 42
>UniRef50_Q5C7K1 Cluster: SJCHGC05550 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC05550 protein - Schistosoma
japonicum (Blood fluke)
Length = 207
Score = 72.1 bits (169), Expect = 1e-11
Identities = 35/94 (37%), Positives = 55/94 (58%), Gaps = 1/94 (1%)
Frame = +3
Query: 234 SIYRTISHFVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSA-ECLCSVT 410
+I+ T ++F + V +G V F QRKW +S+ + +DV+P+ + A ECL S
Sbjct: 13 AIHTTADKYIFRGGSHENVPQGKVAFGLAQRKWGNISVDEPLDVQPYTFNLARECLASAV 72
Query: 411 LEADFMMKKTTSTDPYDSEQMARDFLIQFANQVL 512
L DF KK + +P DS++MA +F +QFA+ L
Sbjct: 73 LAVDFNNKKMSINEPVDSDKMALEFSMQFADTPL 106
>UniRef50_Q9P7Q4 Cluster: Vesicular-fusion protein SEC18 homolog;
n=19; Ascomycota|Rep: Vesicular-fusion protein SEC18
homolog - Schizosaccharomyces pombe (Fission yeast)
Length = 792
Score = 66.1 bits (154), Expect = 8e-10
Identities = 33/86 (38%), Positives = 50/86 (58%), Gaps = 3/86 (3%)
Frame = +3
Query: 261 VFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTI---DVKPFKPSSAECLCSVTLEADFMM 431
VFS + G +G +G S P R+WA+ S+ Q + D P+ P A L S+TLE DF
Sbjct: 96 VFSTKPVPGFPQGCLGASQPHREWASWSLNQQVHVADYDPYGPHGAPYLHSMTLEVDFQN 155
Query: 432 KKTTSTDPYDSEQMARDFLIQFANQV 509
+ T+ +P+D E+MA+ F + +QV
Sbjct: 156 RNRTTNEPFDGEEMAKLFCSSYQSQV 181
>UniRef50_Q5KEU7 Cluster: Vesicular-fusion protein sec18, putative;
n=5; Dikarya|Rep: Vesicular-fusion protein sec18,
putative - Cryptococcus neoformans (Filobasidiella
neoformans)
Length = 844
Score = 56.0 bits (129), Expect = 8e-07
Identities = 32/83 (38%), Positives = 49/83 (59%), Gaps = 3/83 (3%)
Frame = +3
Query: 258 FVFSI-RFYNG-VDRGTVGFSAPQRKWATLS-IGQTIDVKPFKPSSAECLCSVTLEADFM 428
FVFSI R G + G VG S R+W LS +G+T+D++P+ P + E + +E F
Sbjct: 158 FVFSIIRDNTGTLPPGHVGPSKIIRQWVGLSAVGETVDIEPYHPGNGEWASTAEIEVGFR 217
Query: 429 MKKTTSTDPYDSEQMARDFLIQF 497
+K+ + D +DSE+MA F+ F
Sbjct: 218 LKRKETQDIFDSEEMAAAFINAF 240
Score = 38.3 bits (85), Expect = 0.17
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +1
Query: 154 CPSDELAITNCALIHQDDFPSDI 222
CPSD LA+TN ++H DFPSD+
Sbjct: 127 CPSDALALTNRLVVHPSDFPSDV 149
>UniRef50_Q9P4C9 Cluster: Sec18; n=1; Pichia pastoris|Rep: Sec18 -
Pichia pastoris (Yeast)
Length = 762
Score = 51.2 bits (117), Expect = 2e-05
Identities = 29/90 (32%), Positives = 45/90 (50%), Gaps = 4/90 (4%)
Frame = +3
Query: 252 SHFVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPF----KPSSAECLCSVTLEA 419
+ +V + + N ++ GTVG QR WA+ SIGQ I V F L S+ LE
Sbjct: 50 NRYVLTTKTSNKLEPGTVGAYINQRLWASWSIGQEIPVSSFDMFKSTGQQSYLGSMNLEI 109
Query: 420 DFMMKKTTSTDPYDSEQMARDFLIQFANQV 509
F K + +D E++A F+ +F +Q+
Sbjct: 110 SFRSKGKATQREFDQEELAEQFIARFESQI 139
>UniRef50_P18759 Cluster: Vesicular-fusion protein SEC18; n=5;
Saccharomycetales|Rep: Vesicular-fusion protein SEC18 -
Saccharomyces cerevisiae (Baker's yeast)
Length = 758
Score = 48.8 bits (111), Expect = 1e-04
Identities = 28/97 (28%), Positives = 51/97 (52%), Gaps = 5/97 (5%)
Frame = +3
Query: 234 SIYRTISH-FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKP---FKPSSAEC-L 398
+IY I + FVF+ R N + GT+GF+ QR W S+ Q + K FK S + L
Sbjct: 55 NIYIIIDNLFVFTTRHSNDIPPGTIGFNGNQRTWGGWSLNQDVQAKAFDLFKYSGKQSYL 114
Query: 399 CSVTLEADFMMKKTTSTDPYDSEQMARDFLIQFANQV 509
S+ ++ F + + +D +++A+ F+ + +Q+
Sbjct: 115 GSIDIDISFRARGKAVSTVFDQDELAKQFVRCYESQI 151
>UniRef50_Q677Q6 Cluster: Cell division protein 48; n=1;
Lymphocystis disease virus - isolate China|Rep: Cell
division protein 48 - Lymphocystis disease virus -
isolate China
Length = 690
Score = 47.2 bits (107), Expect = 4e-04
Identities = 25/84 (29%), Positives = 47/84 (55%)
Frame = +3
Query: 261 VFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSAECLCSVTLEADFMMKKT 440
+F+++ Y + +GF++ QRKW L IG+T++V + +TLE DF+ K
Sbjct: 41 IFAVKHYP-FEEDVIGFNSFQRKWLNLPIGETVEV--IEKCMEYKFDELTLEIDFLNKIK 97
Query: 441 TSTDPYDSEQMARDFLIQFANQVL 512
++ Y+ E ++ +F +F +Q L
Sbjct: 98 ADSNLYNVEILSNEFKCKFKDQPL 121
>UniRef50_P34732 Cluster: Vesicular-fusion protein SEC18; n=6;
Saccharomycetales|Rep: Vesicular-fusion protein SEC18 -
Candida albicans (Yeast)
Length = 794
Score = 47.2 bits (107), Expect = 4e-04
Identities = 25/89 (28%), Positives = 46/89 (51%), Gaps = 4/89 (4%)
Frame = +3
Query: 258 FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPF----KPSSAECLCSVTLEADF 425
FV+SI + V GT+G + R W S+GQ ++V+ + + L ++ L DF
Sbjct: 86 FVYSIAKDDRVRPGTIGLAGNMRTWGKWSLGQPVNVENYNIFHNGQQQQYLGAIDLSIDF 145
Query: 426 MMKKTTSTDPYDSEQMARDFLIQFANQVL 512
K +++P + +++ FL + NQ+L
Sbjct: 146 RAKARANSNPINHDELVALFLKNYENQIL 174
>UniRef50_Q9M0Y8 Cluster: Vesicle-fusing ATPase; n=9;
Viridiplantae|Rep: Vesicle-fusing ATPase - Arabidopsis
thaliana (Mouse-ear cress)
Length = 742
Score = 45.6 bits (103), Expect = 0.001
Identities = 25/85 (29%), Positives = 44/85 (51%)
Frame = +3
Query: 258 FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSAECLCSVTLEADFMMKK 437
F+ S+ + + G + +A QR+ A +S G + V F P L +TLE +F +KK
Sbjct: 54 FILSLCGHGSIRDGNIALNAIQRRHARVSTGDMVSVSRFVPPENFDLAMLTLELEF-VKK 112
Query: 438 TTSTDPYDSEQMARDFLIQFANQVL 512
T ++ D+ ++ ++ NQVL
Sbjct: 113 GTKSEQVDAALLSTQLKRKYTNQVL 137
>UniRef50_A6XPW2 Cluster: Putative uncharacterized protein; n=2;
Botryotinia fuckeliana B05.10|Rep: Putative
uncharacterized protein - Botryotinia fuckeliana B05.10
Length = 405
Score = 44.4 bits (100), Expect = 0.003
Identities = 26/87 (29%), Positives = 41/87 (47%), Gaps = 3/87 (3%)
Frame = +3
Query: 258 FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVK---PFKPSSAECLCSVTLEADFM 428
FV + R +G G + S PQR W + + I+ + PF L ++ +E F
Sbjct: 123 FVVTARPLDGFPPGCISLSDPQRTWCNVGMLDRINAETYDPFYQGPQAYLGALDVEVGFA 182
Query: 429 MKKTTSTDPYDSEQMARDFLIQFANQV 509
K + PYD +++A F+ F NQV
Sbjct: 183 SAKKFTDVPYDQDELASVFIKWFENQV 209
>UniRef50_Q5B8N2 Cluster: Putative uncharacterized protein; n=1;
Emericella nidulans|Rep: Putative uncharacterized
protein - Emericella nidulans (Aspergillus nidulans)
Length = 775
Score = 41.9 bits (94), Expect = 0.014
Identities = 25/87 (28%), Positives = 40/87 (45%), Gaps = 3/87 (3%)
Frame = +3
Query: 258 FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVK---PFKPSSAECLCSVTLEADFM 428
+VFS R + G +G S+ QR WA ++DV+ PF+ L S +E F
Sbjct: 113 YVFSARILDEFPPGYIGLSSIQRPWARAGFRDSLDVRIYDPFRQGGEAYLGSADMEVKFA 172
Query: 429 MKKTTSTDPYDSEQMARDFLIQFANQV 509
K T YD +++ + F +Q+
Sbjct: 173 GKLRPDT-LYDQDELLNSVIKNFESQI 198
>UniRef50_Q6CBG2 Cluster: Yarrowia lipolytica chromosome C of strain
CLIB122 of Yarrowia lipolytica; n=2;
Saccharomycetales|Rep: Yarrowia lipolytica chromosome C
of strain CLIB122 of Yarrowia lipolytica - Yarrowia
lipolytica (Candida lipolytica)
Length = 1606
Score = 35.9 bits (79), Expect = 0.93
Identities = 19/54 (35%), Positives = 31/54 (57%), Gaps = 1/54 (1%)
Frame = +2
Query: 470 NGQRFPYSVRQSSFTVGQQLAFLFQEKKVL-SLIVKNLEAVDVQALAAGANAVP 628
N Q S+R + F + Q+L +F+ + S+ +KN+EAV+ A AA A +P
Sbjct: 1485 NAQFIVISLRNNMFELAQRLVGIFKVNNMTKSVTIKNVEAVEAAAAAAAAAKIP 1538
>UniRef50_Q5XH72 Cluster: LOC495051 protein; n=5; Tetrapoda|Rep:
LOC495051 protein - Xenopus laevis (African clawed frog)
Length = 1034
Score = 35.5 bits (78), Expect = 1.2
Identities = 18/52 (34%), Positives = 28/52 (53%), Gaps = 3/52 (5%)
Frame = +3
Query: 252 SHFVFSIRFYNGVDRG---TVGFSAPQRKWATLSIGQTIDVKPFKPSSAECL 398
SHF FS+ FYN D+G VG Q +++G + + P+KP + C+
Sbjct: 31 SHFGFSMDFYNTADQGMSIVVGAPRMQTSQRNVTMGGGVFLCPWKPKGSSCV 82
>UniRef50_UPI0000ECB9E1 Cluster: Apoptosis inhibitor 5 (API-5).;
n=3; Amniota|Rep: Apoptosis inhibitor 5 (API-5). -
Gallus gallus
Length = 458
Score = 33.5 bits (73), Expect = 4.9
Identities = 13/39 (33%), Positives = 28/39 (71%)
Frame = +2
Query: 545 EKKVLSLIVKNLEAVDVQALAAGANAVPRRVRMGRLLPD 661
++ +++ I+K++ A +Q +AA A A+ ++ R G+LLP+
Sbjct: 325 DRGLITPIIKDVPAKGIQEIAASAKALAKKARDGKLLPE 363
>UniRef50_A4UHE0 Cluster: Putative uncharacterized protein; n=1;
Alexandrium fundyense|Rep: Putative uncharacterized
protein - Alexandrium fundyense (Dinoflagellate)
Length = 303
Score = 33.5 bits (73), Expect = 4.9
Identities = 21/93 (22%), Positives = 43/93 (46%), Gaps = 1/93 (1%)
Frame = +3
Query: 237 IYRTISHFVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSAECLCSVTLE 416
IY I +FS+ + V+ GT+ + QR + +++ + + V+ P L S LE
Sbjct: 144 IYTEICGHIFSVEAHPWVEVGTLALNKVQRDYLQVAMQEDVFVRHIVPPEDADLISCVLE 203
Query: 417 ADFMMKKTTSTDPYD-SEQMARDFLIQFANQVL 512
D +++ + +EQ + + Q Q++
Sbjct: 204 VDAFVQQGLMVERRSLTEQAVKQTVHQLFGQII 236
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 624,367,235
Number of Sequences: 1657284
Number of extensions: 11834531
Number of successful extensions: 30768
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 29693
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30707
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 53719013270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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