Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20360
         (688 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1834.11c |sec18||secretory pathway protein Sec18 |Schizosacc...    66   4e-12
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces...    27   1.9  
SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone...    27   3.4  
SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe...    27   3.4  
SPBC32H8.09 |||WD repeat protein, human WDR8 family|Schizosaccha...    26   5.9  
SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit...    25   7.8  

>SPAC1834.11c |sec18||secretory pathway protein Sec18
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 792

 Score = 66.1 bits (154), Expect = 4e-12
 Identities = 33/86 (38%), Positives = 50/86 (58%), Gaps = 3/86 (3%)
 Frame = +3

Query: 261 VFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTI---DVKPFKPSSAECLCSVTLEADFMM 431
           VFS +   G  +G +G S P R+WA+ S+ Q +   D  P+ P  A  L S+TLE DF  
Sbjct: 96  VFSTKPVPGFPQGCLGASQPHREWASWSLNQQVHVADYDPYGPHGAPYLHSMTLEVDFQN 155

Query: 432 KKTTSTDPYDSEQMARDFLIQFANQV 509
           +  T+ +P+D E+MA+ F   + +QV
Sbjct: 156 RNRTTNEPFDGEEMAKLFCSSYQSQV 181


>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1727

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 19/36 (52%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
 Frame = -3

Query: 572  LRSTTRLSSLGTR-KPAVVRQ*NLIGELNKEISGHL 468
            L S+T  SS  +R K  +V + NLI ELN+EI GHL
Sbjct: 1309 LNSSTSTSSDASRLKNELVSKENLIEELNQEI-GHL 1343


>SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 809

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 12/24 (50%), Positives = 16/24 (66%)
 Frame = -3

Query: 578 NFLRSTTRLSSLGTRKPAVVRQ*N 507
           N LRS  + S +G RKPA +R+ N
Sbjct: 688 NTLRSARKASPIGDRKPAFMRRAN 711


>SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 317

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 16/57 (28%), Positives = 28/57 (49%)
 Frame = -2

Query: 684 ALSNCTEASGSSRPIRTRRGTALAPAAKACTSTASKFFTINDKTFFSWNKKASCCPT 514
           A S CT ++ S     T   T++AP+     +++S F T +  T  S +  +S  P+
Sbjct: 110 ACSTCTISTSSLSYSGTISSTSIAPSMIGTRTSSSYFITSSSSTPSSSSSSSSSSPS 166


>SPBC32H8.09 |||WD repeat protein, human WDR8
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 483

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 12/29 (41%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
 Frame = +1

Query: 442 HQRIHMIRNKWPEISLFS-SPIKFYCRTT 525
           +Q++H +R  +PE S  S S I+F C  T
Sbjct: 303 YQKVHKLRTDFPEPSFCSASKIRFNCDET 331


>SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit
           Skp1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 161

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 13/37 (35%), Positives = 23/37 (62%)
 Frame = +3

Query: 414 EADFMMKKTTSTDPYDSEQMARDFLIQFANQVLLSDN 524
           E+D  +KK+T  D +D + MA D  + F  +++L+ N
Sbjct: 72  ESDIRLKKSTDIDEWDRKFMAVDQEMLF--EIVLASN 106


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,617,161
Number of Sequences: 5004
Number of extensions: 51687
Number of successful extensions: 113
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)