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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20360
         (688 letters)

Database: human 
           237,096 sequences; 76,859,062 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U03985-1|AAA17411.1|  751|Homo sapiens protein ( Human N-ethylma...    82   2e-15
AF135168-1|AAF70545.1|  744|Homo sapiens N-ethylmaleimide-sensit...    82   2e-15
AF102846-1|AAF04745.2|  744|Homo sapiens N-ethylmaleimide-sensit...    82   2e-15
BC030613-1|AAH30613.1|  744|Homo sapiens N-ethylmaleimide-sensit...    82   2e-15
Y13145-1|CAA73606.1|  501|Homo sapiens protein X protein.              31   5.1  
U82328-1|AAC39661.1|  501|Homo sapiens pyruvate dehydrogenase co...    31   5.1  
U79296-1|AAB50223.1|  375|Homo sapiens dihyrolipoamide acetyl tr...    31   5.1  
AJ298105-1|CAC18649.1|  501|Homo sapiens lipoyl-containing compo...    31   5.1  
AF001437-1|AAB66315.1|  501|Homo sapiens dihydrolipoamide dehydr...    31   5.1  
BC010389-1|AAH10389.1|  501|Homo sapiens pyruvate dehydrogenase ...    30   8.9  

>U03985-1|AAA17411.1|  751|Homo sapiens protein ( Human
           N-ethylmaleimide-sensitive factor mRNA, partial cds. ).
          Length = 751

 Score = 82.2 bits (194), Expect = 2e-15
 Identities = 36/84 (42%), Positives = 59/84 (70%), Gaps = 1/84 (1%)
 Frame = +3

Query: 258 FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSA-ECLCSVTLEADFMMK 434
           + F+++ +  V  G++ FS PQRKWA LSIGQ I+V  +    A +C+ ++T+E DF+ K
Sbjct: 52  YTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQK 111

Query: 435 KTTSTDPYDSEQMARDFLIQFANQ 506
           K+  ++PYD+++MA +F+ QF NQ
Sbjct: 112 KSNDSNPYDTDKMAAEFIQQFNNQ 135



 Score = 43.6 bits (98), Expect = 7e-04
 Identities = 16/34 (47%), Positives = 27/34 (79%)
 Frame = +1

Query: 154 CPSDELAITNCALIHQDDFPSDIKHIEVSTGPSH 255
           CP+DEL++TNC+++++ DF S  +H+ V T P+H
Sbjct: 18  CPTDELSLTNCSVVNEKDFQSG-QHVIVRTSPNH 50


>AF135168-1|AAF70545.1|  744|Homo sapiens N-ethylmaleimide-sensitive
           factor protein.
          Length = 744

 Score = 82.2 bits (194), Expect = 2e-15
 Identities = 36/84 (42%), Positives = 59/84 (70%), Gaps = 1/84 (1%)
 Frame = +3

Query: 258 FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSA-ECLCSVTLEADFMMK 434
           + F+++ +  V  G++ FS PQRKWA LSIGQ I+V  +    A +C+ ++T+E DF+ K
Sbjct: 45  YTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQK 104

Query: 435 KTTSTDPYDSEQMARDFLIQFANQ 506
           K+  ++PYD+++MA +F+ QF NQ
Sbjct: 105 KSNDSNPYDTDKMAAEFIQQFNNQ 128



 Score = 43.6 bits (98), Expect = 7e-04
 Identities = 16/34 (47%), Positives = 27/34 (79%)
 Frame = +1

Query: 154 CPSDELAITNCALIHQDDFPSDIKHIEVSTGPSH 255
           CP+DEL++TNC+++++ DF S  +H+ V T P+H
Sbjct: 11  CPTDELSLTNCSVVNEKDFQSG-QHVIVRTSPNH 43


>AF102846-1|AAF04745.2|  744|Homo sapiens N-ethylmaleimide-sensitive
           factor protein.
          Length = 744

 Score = 82.2 bits (194), Expect = 2e-15
 Identities = 36/84 (42%), Positives = 59/84 (70%), Gaps = 1/84 (1%)
 Frame = +3

Query: 258 FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSA-ECLCSVTLEADFMMK 434
           + F+++ +  V  G++ FS PQRKWA LSIGQ I+V  +    A +C+ ++T+E DF+ K
Sbjct: 45  YTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQK 104

Query: 435 KTTSTDPYDSEQMARDFLIQFANQ 506
           K+  ++PYD+++MA +F+ QF NQ
Sbjct: 105 KSNDSNPYDTDKMAAEFIQQFNNQ 128



 Score = 44.8 bits (101), Expect = 3e-04
 Identities = 17/34 (50%), Positives = 27/34 (79%)
 Frame = +1

Query: 154 CPSDELAITNCALIHQDDFPSDIKHIEVSTGPSH 255
           CP+DEL++TNCA++++ DF S  +H+ V T P+H
Sbjct: 11  CPTDELSLTNCAVVNEKDFQSG-QHVIVRTSPNH 43


>BC030613-1|AAH30613.1|  744|Homo sapiens N-ethylmaleimide-sensitive
           factor protein.
          Length = 744

 Score = 81.8 bits (193), Expect = 2e-15
 Identities = 36/84 (42%), Positives = 59/84 (70%), Gaps = 1/84 (1%)
 Frame = +3

Query: 258 FVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSA-ECLCSVTLEADFMMK 434
           + F+++ +  V  G++ FS PQRKWA LSIGQ I+V  +    A +C+ ++T+E DF+ K
Sbjct: 45  YTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQK 104

Query: 435 KTTSTDPYDSEQMARDFLIQFANQ 506
           K+  ++PYD+++MA +F+ QF NQ
Sbjct: 105 KSIDSNPYDTDKMAAEFIQQFNNQ 128



 Score = 44.8 bits (101), Expect = 3e-04
 Identities = 17/34 (50%), Positives = 27/34 (79%)
 Frame = +1

Query: 154 CPSDELAITNCALIHQDDFPSDIKHIEVSTGPSH 255
           CP+DEL++TNCA++++ DF S  +H+ V T P+H
Sbjct: 11  CPTDELSLTNCAVVNEKDFQSG-QHVIVRTSPNH 43


>Y13145-1|CAA73606.1|  501|Homo sapiens protein X protein.
          Length = 501

 Score = 30.7 bits (66), Expect = 5.1
 Identities = 13/39 (33%), Positives = 25/39 (64%)
 Frame = +2

Query: 545 EKKVLSLIVKNLEAVDVQALAAGANAVPRRVRMGRLLPD 661
           +K +L+ I+K+  A  +Q +A    A+ ++ R G+LLP+
Sbjct: 370 DKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPE 408


>U82328-1|AAC39661.1|  501|Homo sapiens pyruvate dehydrogenase
           complex protein X subunit precursor protein.
          Length = 501

 Score = 30.7 bits (66), Expect = 5.1
 Identities = 13/39 (33%), Positives = 25/39 (64%)
 Frame = +2

Query: 545 EKKVLSLIVKNLEAVDVQALAAGANAVPRRVRMGRLLPD 661
           +K +L+ I+K+  A  +Q +A    A+ ++ R G+LLP+
Sbjct: 370 DKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPE 408


>U79296-1|AAB50223.1|  375|Homo sapiens dihyrolipoamide acetyl
           transferase protein.
          Length = 375

 Score = 30.7 bits (66), Expect = 5.1
 Identities = 13/39 (33%), Positives = 25/39 (64%)
 Frame = +2

Query: 545 EKKVLSLIVKNLEAVDVQALAAGANAVPRRVRMGRLLPD 661
           +K +L+ I+K+  A  +Q +A    A+ ++ R G+LLP+
Sbjct: 244 DKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPE 282


>AJ298105-1|CAC18649.1|  501|Homo sapiens lipoyl-containing
           component X protein.
          Length = 501

 Score = 30.7 bits (66), Expect = 5.1
 Identities = 13/39 (33%), Positives = 25/39 (64%)
 Frame = +2

Query: 545 EKKVLSLIVKNLEAVDVQALAAGANAVPRRVRMGRLLPD 661
           +K +L+ I+K+  A  +Q +A    A+ ++ R G+LLP+
Sbjct: 370 DKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPE 408


>AF001437-1|AAB66315.1|  501|Homo sapiens dihydrolipoamide
           dehydrogenase-binding protein protein.
          Length = 501

 Score = 30.7 bits (66), Expect = 5.1
 Identities = 13/39 (33%), Positives = 25/39 (64%)
 Frame = +2

Query: 545 EKKVLSLIVKNLEAVDVQALAAGANAVPRRVRMGRLLPD 661
           +K +L+ I+K+  A  +Q +A    A+ ++ R G+LLP+
Sbjct: 370 DKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPE 408


>BC010389-1|AAH10389.1|  501|Homo sapiens pyruvate dehydrogenase
           complex, component X protein.
          Length = 501

 Score = 29.9 bits (64), Expect = 8.9
 Identities = 13/38 (34%), Positives = 24/38 (63%)
 Frame = +2

Query: 548 KKVLSLIVKNLEAVDVQALAAGANAVPRRVRMGRLLPD 661
           K +L+ I+K+  A  +Q +A    A+ ++ R G+LLP+
Sbjct: 371 KGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPE 408


  Database: human
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 76,859,062
  Number of sequences in database:  237,096
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 90,107,611
Number of Sequences: 237096
Number of extensions: 1797636
Number of successful extensions: 4207
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3992
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4203
length of database: 76,859,062
effective HSP length: 88
effective length of database: 55,994,614
effective search space used: 7839245960
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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