SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20358
         (758 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g46696.1 68414.m05216 hypothetical protein slight similarity ...    32   0.48 
At4g39320.1 68417.m05567 microtubule-associated protein-related ...    31   0.63 
At3g04310.1 68416.m00456 expressed protein                             31   1.1  
At1g35220.1 68414.m04368 expressed protein                             30   1.5  
At1g43770.1 68414.m05040 PHD finger family protein contains Pfam...    30   1.9  
At5g07570.1 68418.m00867 glycine/proline-rich protein contains s...    29   2.5  
At5g28600.1 68418.m03490 hypothetical protein                          29   3.4  
At3g20860.1 68416.m02637 protein kinase family protein contains ...    29   3.4  
At2g36010.3 68415.m04422 E2F transcription factor-3 (E2F3) ident...    29   3.4  
At2g36010.2 68415.m04421 E2F transcription factor-3 (E2F3) ident...    29   3.4  
At2g36010.1 68415.m04420 E2F transcription factor-3 (E2F3) ident...    29   3.4  
At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /...    29   3.4  
At5g16520.1 68418.m01932 expressed protein                             29   4.4  
At5g01140.1 68418.m00018 hypothetical protein contains Pfam prof...    29   4.4  
At5g15510.1 68418.m01816 expressed protein                             28   5.9  
At2g35050.1 68415.m04300 protein kinase family protein contains ...    28   5.9  
At1g66060.1 68414.m07498 hypothetical protein contains Pfam PF04...    28   5.9  
At1g66000.1 68414.m07490 expressed protein contains Pfam PF04510...    28   5.9  

>At1g46696.1 68414.m05216 hypothetical protein slight similarity to
           maebl (GI:20087019)[Plasmodium falciparum].
          Length = 616

 Score = 31.9 bits (69), Expect = 0.48
 Identities = 16/53 (30%), Positives = 32/53 (60%), Gaps = 3/53 (5%)
 Frame = +3

Query: 600 YTADYGQEVKETTNIPETYETTAADTK---DITTELAKGEIPDITSIQDDMKS 749
           +++ YG   KE+  + ET +  AA+ K   ++  E+ KGEIPD++   + +++
Sbjct: 488 FSSKYGNFFKESEEV-ETLKVRAAEAKANRELLEEIEKGEIPDLSKELESVRA 539


>At4g39320.1 68417.m05567 microtubule-associated protein-related
           contains weak similarity to microtubule-associated
           protein 1B (MAP 1B) (Swiss-Prot:P46821) [Homo sapiens]
          Length = 166

 Score = 31.5 bits (68), Expect = 0.63
 Identities = 20/55 (36%), Positives = 30/55 (54%), Gaps = 2/55 (3%)
 Frame = +2

Query: 26  DSTSETVVQDQEITTA-IPVSTKL-TEYPETTQKTFEAETPATDTTESDEDVKAP 184
           D   +   +D++IT   IPV+  L T   E    T EA++PAT+T  S  + +AP
Sbjct: 45  DKADKITKEDEKITPEKIPVTDDLKTALEEENTPTKEAKSPATETKYSVTEKRAP 99


>At3g04310.1 68416.m00456 expressed protein
          Length = 269

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 15/53 (28%), Positives = 31/53 (58%)
 Frame = -1

Query: 518 AIYYSLFSSISFVIPIEGISVVTIGDFSASVTVLCSSDTEVVLKIALSVVWSK 360
           ++ + +F ++S+++   GIS++   D +A V  L     + VL + +S VWS+
Sbjct: 94  SMVFEVFRTLSWMLAPIGISLLLGTDSNAGVMALAVPLVQSVLSLVVSKVWSR 146


>At1g35220.1 68414.m04368 expressed protein
          Length = 1028

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 17/64 (26%), Positives = 34/64 (53%), Gaps = 3/64 (4%)
 Frame = +2

Query: 20  TGDSTSETVVQDQEITTA---IPVSTKLTEYPETTQKTFEAETPATDTTESDEDVKAPVK 190
           +GDS +  +++   ++ A   +P+ST+    PE+T+    + TP+ DTT   E   + + 
Sbjct: 504 SGDSLTSQIIEASMVSDAPQEVPLSTE--HVPESTKHEAASSTPSVDTTALTETFSSNLN 561

Query: 191 TEFE 202
            + E
Sbjct: 562 LQNE 565


>At1g43770.1 68414.m05040 PHD finger family protein contains Pfam
           domain, PF00628: PHD-finger
          Length = 371

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = +1

Query: 244 C*FTSEHEVHEKTTDVPTTEFVTSYSKLPENAET-SDISDLDQT 372
           C F S H      T +P TE++T   +  +N+E+ SD +++DQT
Sbjct: 28  CMFESIHRYCLGITPIPFTEYITWICEDCDNSESDSDCNEVDQT 71


>At5g07570.1 68418.m00867 glycine/proline-rich protein contains
           similarity to flagelliform silk protein [Nephila
           clavipes] gi|7106224|gb|AAF36090
          Length = 1504

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 10/28 (35%), Positives = 16/28 (57%)
 Frame = -2

Query: 160 FCSVSCRCFSLKCFLCCFWIFCQLCRYW 77
           FC   C  F  +C+ CC++ F   CR++
Sbjct: 28  FCCGLCGRFWFRCWFCCWFQFWFRCRFY 55


>At5g28600.1 68418.m03490 hypothetical protein
          Length = 711

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 7/65 (10%)
 Frame = +1

Query: 259 EHEVHEKTTDVPTTEFVTSYSKLPENAETSD-----ISDLDQTTDSAILSTT--SVSDEH 417
           E +V+E+   +P TE VTS     +N ET+       +DL+ +    I+S T    S +H
Sbjct: 461 EGDVNERPDIIPVTEVVTSPKHSGDNEETTTDMDPLANDLEGSKRPEIISATEAGTSPKH 520

Query: 418 KTVTE 432
             V E
Sbjct: 521 SIVNE 525


>At3g20860.1 68416.m02637 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069; contains
           serine/threonine protein kinase domain,
           INTERPRO:IPR002290
          Length = 427

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
 Frame = +2

Query: 59  EITTAIPVSTKLTEYP-ETTQKTFEAETPATDTTESDEDVKAPVKTEFEKATSSPVLTST 235
           E  T +P  TK  + P +  Q++ E E    DT+ S +D + P K++ E +  S +    
Sbjct: 295 ENRTGLPPKTKSAKTPIKHNQESEETEKKNKDTSSSSKDKERPAKSQ-EMSVISTLTLLR 353

Query: 236 EF 241
           EF
Sbjct: 354 EF 355


>At2g36010.3 68415.m04422 E2F transcription factor-3 (E2F3)
           identical to E2F transcription factor-3 E2F3
           [Arabidopsis thaliana] gi|10443853|gb|AAG17610
          Length = 485

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 14/50 (28%), Positives = 23/50 (46%)
 Frame = +1

Query: 253 TSEHEVHEKTTDVPTTEFVTSYSKLPENAETSDISDLDQTTDSAILSTTS 402
           T  H++   T D P T  V+     P+  +TSD++ L +     +  T S
Sbjct: 378 TGHHDIEALTVDNPETAIVSHDHPHPQPGDTSDLNYLQEQVGGMLKITPS 427


>At2g36010.2 68415.m04421 E2F transcription factor-3 (E2F3)
           identical to E2F transcription factor-3 E2F3
           [Arabidopsis thaliana] gi|10443853|gb|AAG17610
          Length = 514

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 14/50 (28%), Positives = 23/50 (46%)
 Frame = +1

Query: 253 TSEHEVHEKTTDVPTTEFVTSYSKLPENAETSDISDLDQTTDSAILSTTS 402
           T  H++   T D P T  V+     P+  +TSD++ L +     +  T S
Sbjct: 407 TGHHDIEALTVDNPETAIVSHDHPHPQPGDTSDLNYLQEQVGGMLKITPS 456


>At2g36010.1 68415.m04420 E2F transcription factor-3 (E2F3)
           identical to E2F transcription factor-3 E2F3
           [Arabidopsis thaliana] gi|10443853|gb|AAG17610
          Length = 483

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 14/50 (28%), Positives = 23/50 (46%)
 Frame = +1

Query: 253 TSEHEVHEKTTDVPTTEFVTSYSKLPENAETSDISDLDQTTDSAILSTTS 402
           T  H++   T D P T  V+     P+  +TSD++ L +     +  T S
Sbjct: 376 TGHHDIEALTVDNPETAIVSHDHPHPQPGDTSDLNYLQEQVGGMLKITPS 425


>At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /
           phosphoglyceride transfer family protein contains Pfam
           PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
           CRAL/TRIO, N-terminus; similar to SEC14-like protein 2
           (Alpha-tocopherol associated protein) (TAP) (bTAP)
           (Fragment) (SP:P58875)  {Bos taurus}
          Length = 683

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 27/77 (35%), Positives = 37/77 (48%), Gaps = 2/77 (2%)
 Frame = +2

Query: 56  QEITTAIPVSTKLTEYPETTQKTFEAETPATDTTESDEDVK-APVKTEF-EKATSSPVLT 229
           +EI  A PV+T+ T+  E      E    A  TTE+ E+ K APV TE  E+  ++P  T
Sbjct: 212 EEILPAAPVTTE-TKVEEKVVPV-ETTPAAPVTTETKEEEKAAPVTTETKEEEKAAPGET 269

Query: 230 STEFNANLLVNMKYMKK 280
             E  A     +K   K
Sbjct: 270 KKEEKATASTQVKRASK 286



 Score = 29.1 bits (62), Expect = 3.4
 Identities = 23/84 (27%), Positives = 40/84 (47%), Gaps = 1/84 (1%)
 Frame = +2

Query: 32  TSETVVQDQEITTAIPVSTKLTEYPETTQKTFEAETPATDTTESDEDVKAPVKTEFEKAT 211
           T+ET V+++     +PV T       T  K  E   P T  T+ +E        + EKAT
Sbjct: 221 TTETKVEEK----VVPVETTPAAPVTTETKEEEKAAPVTTETKEEEKAAPGETKKEEKAT 276

Query: 212 -SSPVLTSTEFNANLLVNMKYMKK 280
            S+ V  +++F  ++ V++   +K
Sbjct: 277 ASTQVKRASKFIKDIFVSVTTSEK 300


>At5g16520.1 68418.m01932 expressed protein 
          Length = 608

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 13/33 (39%), Positives = 17/33 (51%)
 Frame = -1

Query: 347 EVSAFSGNLEYDVTNSVVGTSVVFSCTSCSLVN 249
           +V+A S    Y   N+  G +V F CT C L N
Sbjct: 216 KVAALSSLGSYTCKNTTSGPTVNFKCTKCRLTN 248


>At5g01140.1 68418.m00018 hypothetical protein contains Pfam profile
           PF05056: Protein of unknown function (DUF674)
          Length = 473

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 10/21 (47%), Positives = 15/21 (71%)
 Frame = -2

Query: 133 SLKCFLCCFWIFCQLCRYWNS 71
           SLKCF C F+  C++C  +N+
Sbjct: 96  SLKCFGCRFFSICRMCSNFNT 116


>At5g15510.1 68418.m01816 expressed protein
          Length = 497

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 6/67 (8%)
 Frame = +2

Query: 17  KTGDSTSETVVQD--QEITTAIPVSTKLTEYPETTQKT--FEAETPAT--DTTESDEDVK 178
           KTG ST +       +E   ++PVS K+    E  +K   F     A   +  +++ED K
Sbjct: 172 KTGVSTMKRSRAKVVEEARQSVPVSGKVMNLVEAFEKLTCFSNSKTANKIEENQTEEDTK 231

Query: 179 APVKTEF 199
            PVK EF
Sbjct: 232 KPVKLEF 238


>At2g35050.1 68415.m04300 protein kinase family protein contains
           Pfam profile: PF00069 Eukaryotic protein kinase domain
          Length = 1257

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 17/58 (29%), Positives = 29/58 (50%), Gaps = 9/58 (15%)
 Frame = +2

Query: 11  ESKTGDSTSETVVQDQEITTAIPVSTKLTE---------YPETTQKTFEAETPATDTT 157
           E      +S+++V DQ +    P+S K++          YPE + +T + +T A DTT
Sbjct: 581 EYSVSSVSSDSMVPDQALKEEAPISMKISNSTPDPKSLVYPEKSLRTSQEKTGAFDTT 638


>At1g66060.1 68414.m07498 hypothetical protein contains Pfam PF04510
           : Family of unknown function (DUF577); common family
           comprised of At4g09440, At1g66000, At1g66060
          Length = 315

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 11/21 (52%), Positives = 14/21 (66%)
 Frame = +1

Query: 292 PTTEFVTSYSKLPENAETSDI 354
           PT EF+ + +K PEN E  DI
Sbjct: 30  PTVEFIAALTKPPENPEDKDI 50


>At1g66000.1 68414.m07490 expressed protein contains Pfam PF04510 :
           Family of unknown function (DUF577); common family
           comprised of At4g09440, At1g66000, At1g66060
          Length = 315

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 11/21 (52%), Positives = 14/21 (66%)
 Frame = +1

Query: 292 PTTEFVTSYSKLPENAETSDI 354
           PT EF+ + +K PEN E  DI
Sbjct: 30  PTVEFIAALTKPPENPEDKDI 50


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,665,097
Number of Sequences: 28952
Number of extensions: 296444
Number of successful extensions: 1000
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 963
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1000
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -