BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20358 (758 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g46696.1 68414.m05216 hypothetical protein slight similarity ... 32 0.48 At4g39320.1 68417.m05567 microtubule-associated protein-related ... 31 0.63 At3g04310.1 68416.m00456 expressed protein 31 1.1 At1g35220.1 68414.m04368 expressed protein 30 1.5 At1g43770.1 68414.m05040 PHD finger family protein contains Pfam... 30 1.9 At5g07570.1 68418.m00867 glycine/proline-rich protein contains s... 29 2.5 At5g28600.1 68418.m03490 hypothetical protein 29 3.4 At3g20860.1 68416.m02637 protein kinase family protein contains ... 29 3.4 At2g36010.3 68415.m04422 E2F transcription factor-3 (E2F3) ident... 29 3.4 At2g36010.2 68415.m04421 E2F transcription factor-3 (E2F3) ident... 29 3.4 At2g36010.1 68415.m04420 E2F transcription factor-3 (E2F3) ident... 29 3.4 At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /... 29 3.4 At5g16520.1 68418.m01932 expressed protein 29 4.4 At5g01140.1 68418.m00018 hypothetical protein contains Pfam prof... 29 4.4 At5g15510.1 68418.m01816 expressed protein 28 5.9 At2g35050.1 68415.m04300 protein kinase family protein contains ... 28 5.9 At1g66060.1 68414.m07498 hypothetical protein contains Pfam PF04... 28 5.9 At1g66000.1 68414.m07490 expressed protein contains Pfam PF04510... 28 5.9 >At1g46696.1 68414.m05216 hypothetical protein slight similarity to maebl (GI:20087019)[Plasmodium falciparum]. Length = 616 Score = 31.9 bits (69), Expect = 0.48 Identities = 16/53 (30%), Positives = 32/53 (60%), Gaps = 3/53 (5%) Frame = +3 Query: 600 YTADYGQEVKETTNIPETYETTAADTK---DITTELAKGEIPDITSIQDDMKS 749 +++ YG KE+ + ET + AA+ K ++ E+ KGEIPD++ + +++ Sbjct: 488 FSSKYGNFFKESEEV-ETLKVRAAEAKANRELLEEIEKGEIPDLSKELESVRA 539 >At4g39320.1 68417.m05567 microtubule-associated protein-related contains weak similarity to microtubule-associated protein 1B (MAP 1B) (Swiss-Prot:P46821) [Homo sapiens] Length = 166 Score = 31.5 bits (68), Expect = 0.63 Identities = 20/55 (36%), Positives = 30/55 (54%), Gaps = 2/55 (3%) Frame = +2 Query: 26 DSTSETVVQDQEITTA-IPVSTKL-TEYPETTQKTFEAETPATDTTESDEDVKAP 184 D + +D++IT IPV+ L T E T EA++PAT+T S + +AP Sbjct: 45 DKADKITKEDEKITPEKIPVTDDLKTALEEENTPTKEAKSPATETKYSVTEKRAP 99 >At3g04310.1 68416.m00456 expressed protein Length = 269 Score = 30.7 bits (66), Expect = 1.1 Identities = 15/53 (28%), Positives = 31/53 (58%) Frame = -1 Query: 518 AIYYSLFSSISFVIPIEGISVVTIGDFSASVTVLCSSDTEVVLKIALSVVWSK 360 ++ + +F ++S+++ GIS++ D +A V L + VL + +S VWS+ Sbjct: 94 SMVFEVFRTLSWMLAPIGISLLLGTDSNAGVMALAVPLVQSVLSLVVSKVWSR 146 >At1g35220.1 68414.m04368 expressed protein Length = 1028 Score = 30.3 bits (65), Expect = 1.5 Identities = 17/64 (26%), Positives = 34/64 (53%), Gaps = 3/64 (4%) Frame = +2 Query: 20 TGDSTSETVVQDQEITTA---IPVSTKLTEYPETTQKTFEAETPATDTTESDEDVKAPVK 190 +GDS + +++ ++ A +P+ST+ PE+T+ + TP+ DTT E + + Sbjct: 504 SGDSLTSQIIEASMVSDAPQEVPLSTE--HVPESTKHEAASSTPSVDTTALTETFSSNLN 561 Query: 191 TEFE 202 + E Sbjct: 562 LQNE 565 >At1g43770.1 68414.m05040 PHD finger family protein contains Pfam domain, PF00628: PHD-finger Length = 371 Score = 29.9 bits (64), Expect = 1.9 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 1/44 (2%) Frame = +1 Query: 244 C*FTSEHEVHEKTTDVPTTEFVTSYSKLPENAET-SDISDLDQT 372 C F S H T +P TE++T + +N+E+ SD +++DQT Sbjct: 28 CMFESIHRYCLGITPIPFTEYITWICEDCDNSESDSDCNEVDQT 71 >At5g07570.1 68418.m00867 glycine/proline-rich protein contains similarity to flagelliform silk protein [Nephila clavipes] gi|7106224|gb|AAF36090 Length = 1504 Score = 29.5 bits (63), Expect = 2.5 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = -2 Query: 160 FCSVSCRCFSLKCFLCCFWIFCQLCRYW 77 FC C F +C+ CC++ F CR++ Sbjct: 28 FCCGLCGRFWFRCWFCCWFQFWFRCRFY 55 >At5g28600.1 68418.m03490 hypothetical protein Length = 711 Score = 29.1 bits (62), Expect = 3.4 Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 7/65 (10%) Frame = +1 Query: 259 EHEVHEKTTDVPTTEFVTSYSKLPENAETSD-----ISDLDQTTDSAILSTT--SVSDEH 417 E +V+E+ +P TE VTS +N ET+ +DL+ + I+S T S +H Sbjct: 461 EGDVNERPDIIPVTEVVTSPKHSGDNEETTTDMDPLANDLEGSKRPEIISATEAGTSPKH 520 Query: 418 KTVTE 432 V E Sbjct: 521 SIVNE 525 >At3g20860.1 68416.m02637 protein kinase family protein contains protein kinase domain, Pfam:PF00069; contains serine/threonine protein kinase domain, INTERPRO:IPR002290 Length = 427 Score = 29.1 bits (62), Expect = 3.4 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 1/62 (1%) Frame = +2 Query: 59 EITTAIPVSTKLTEYP-ETTQKTFEAETPATDTTESDEDVKAPVKTEFEKATSSPVLTST 235 E T +P TK + P + Q++ E E DT+ S +D + P K++ E + S + Sbjct: 295 ENRTGLPPKTKSAKTPIKHNQESEETEKKNKDTSSSSKDKERPAKSQ-EMSVISTLTLLR 353 Query: 236 EF 241 EF Sbjct: 354 EF 355 >At2g36010.3 68415.m04422 E2F transcription factor-3 (E2F3) identical to E2F transcription factor-3 E2F3 [Arabidopsis thaliana] gi|10443853|gb|AAG17610 Length = 485 Score = 29.1 bits (62), Expect = 3.4 Identities = 14/50 (28%), Positives = 23/50 (46%) Frame = +1 Query: 253 TSEHEVHEKTTDVPTTEFVTSYSKLPENAETSDISDLDQTTDSAILSTTS 402 T H++ T D P T V+ P+ +TSD++ L + + T S Sbjct: 378 TGHHDIEALTVDNPETAIVSHDHPHPQPGDTSDLNYLQEQVGGMLKITPS 427 >At2g36010.2 68415.m04421 E2F transcription factor-3 (E2F3) identical to E2F transcription factor-3 E2F3 [Arabidopsis thaliana] gi|10443853|gb|AAG17610 Length = 514 Score = 29.1 bits (62), Expect = 3.4 Identities = 14/50 (28%), Positives = 23/50 (46%) Frame = +1 Query: 253 TSEHEVHEKTTDVPTTEFVTSYSKLPENAETSDISDLDQTTDSAILSTTS 402 T H++ T D P T V+ P+ +TSD++ L + + T S Sbjct: 407 TGHHDIEALTVDNPETAIVSHDHPHPQPGDTSDLNYLQEQVGGMLKITPS 456 >At2g36010.1 68415.m04420 E2F transcription factor-3 (E2F3) identical to E2F transcription factor-3 E2F3 [Arabidopsis thaliana] gi|10443853|gb|AAG17610 Length = 483 Score = 29.1 bits (62), Expect = 3.4 Identities = 14/50 (28%), Positives = 23/50 (46%) Frame = +1 Query: 253 TSEHEVHEKTTDVPTTEFVTSYSKLPENAETSDISDLDQTTDSAILSTTS 402 T H++ T D P T V+ P+ +TSD++ L + + T S Sbjct: 376 TGHHDIEALTVDNPETAIVSHDHPHPQPGDTSDLNYLQEQVGGMLKITPS 425 >At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus; similar to SEC14-like protein 2 (Alpha-tocopherol associated protein) (TAP) (bTAP) (Fragment) (SP:P58875) {Bos taurus} Length = 683 Score = 29.1 bits (62), Expect = 3.4 Identities = 27/77 (35%), Positives = 37/77 (48%), Gaps = 2/77 (2%) Frame = +2 Query: 56 QEITTAIPVSTKLTEYPETTQKTFEAETPATDTTESDEDVK-APVKTEF-EKATSSPVLT 229 +EI A PV+T+ T+ E E A TTE+ E+ K APV TE E+ ++P T Sbjct: 212 EEILPAAPVTTE-TKVEEKVVPV-ETTPAAPVTTETKEEEKAAPVTTETKEEEKAAPGET 269 Query: 230 STEFNANLLVNMKYMKK 280 E A +K K Sbjct: 270 KKEEKATASTQVKRASK 286 Score = 29.1 bits (62), Expect = 3.4 Identities = 23/84 (27%), Positives = 40/84 (47%), Gaps = 1/84 (1%) Frame = +2 Query: 32 TSETVVQDQEITTAIPVSTKLTEYPETTQKTFEAETPATDTTESDEDVKAPVKTEFEKAT 211 T+ET V+++ +PV T T K E P T T+ +E + EKAT Sbjct: 221 TTETKVEEK----VVPVETTPAAPVTTETKEEEKAAPVTTETKEEEKAAPGETKKEEKAT 276 Query: 212 -SSPVLTSTEFNANLLVNMKYMKK 280 S+ V +++F ++ V++ +K Sbjct: 277 ASTQVKRASKFIKDIFVSVTTSEK 300 >At5g16520.1 68418.m01932 expressed protein Length = 608 Score = 28.7 bits (61), Expect = 4.4 Identities = 13/33 (39%), Positives = 17/33 (51%) Frame = -1 Query: 347 EVSAFSGNLEYDVTNSVVGTSVVFSCTSCSLVN 249 +V+A S Y N+ G +V F CT C L N Sbjct: 216 KVAALSSLGSYTCKNTTSGPTVNFKCTKCRLTN 248 >At5g01140.1 68418.m00018 hypothetical protein contains Pfam profile PF05056: Protein of unknown function (DUF674) Length = 473 Score = 28.7 bits (61), Expect = 4.4 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = -2 Query: 133 SLKCFLCCFWIFCQLCRYWNS 71 SLKCF C F+ C++C +N+ Sbjct: 96 SLKCFGCRFFSICRMCSNFNT 116 >At5g15510.1 68418.m01816 expressed protein Length = 497 Score = 28.3 bits (60), Expect = 5.9 Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 6/67 (8%) Frame = +2 Query: 17 KTGDSTSETVVQD--QEITTAIPVSTKLTEYPETTQKT--FEAETPAT--DTTESDEDVK 178 KTG ST + +E ++PVS K+ E +K F A + +++ED K Sbjct: 172 KTGVSTMKRSRAKVVEEARQSVPVSGKVMNLVEAFEKLTCFSNSKTANKIEENQTEEDTK 231 Query: 179 APVKTEF 199 PVK EF Sbjct: 232 KPVKLEF 238 >At2g35050.1 68415.m04300 protein kinase family protein contains Pfam profile: PF00069 Eukaryotic protein kinase domain Length = 1257 Score = 28.3 bits (60), Expect = 5.9 Identities = 17/58 (29%), Positives = 29/58 (50%), Gaps = 9/58 (15%) Frame = +2 Query: 11 ESKTGDSTSETVVQDQEITTAIPVSTKLTE---------YPETTQKTFEAETPATDTT 157 E +S+++V DQ + P+S K++ YPE + +T + +T A DTT Sbjct: 581 EYSVSSVSSDSMVPDQALKEEAPISMKISNSTPDPKSLVYPEKSLRTSQEKTGAFDTT 638 >At1g66060.1 68414.m07498 hypothetical protein contains Pfam PF04510 : Family of unknown function (DUF577); common family comprised of At4g09440, At1g66000, At1g66060 Length = 315 Score = 28.3 bits (60), Expect = 5.9 Identities = 11/21 (52%), Positives = 14/21 (66%) Frame = +1 Query: 292 PTTEFVTSYSKLPENAETSDI 354 PT EF+ + +K PEN E DI Sbjct: 30 PTVEFIAALTKPPENPEDKDI 50 >At1g66000.1 68414.m07490 expressed protein contains Pfam PF04510 : Family of unknown function (DUF577); common family comprised of At4g09440, At1g66000, At1g66060 Length = 315 Score = 28.3 bits (60), Expect = 5.9 Identities = 11/21 (52%), Positives = 14/21 (66%) Frame = +1 Query: 292 PTTEFVTSYSKLPENAETSDI 354 PT EF+ + +K PEN E DI Sbjct: 30 PTVEFIAALTKPPENPEDKDI 50 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,665,097 Number of Sequences: 28952 Number of extensions: 296444 Number of successful extensions: 1000 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 963 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1000 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1692519896 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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