Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20327
(759 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 192 3e-51
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 192 3e-51
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 182 3e-48
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 177 9e-47
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.1
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.1
AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 9.5
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 9.5
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 192 bits (468), Expect = 3e-51
Identities = 104/171 (60%), Positives = 116/171 (67%), Gaps = 4/171 (2%)
Frame = +2
Query: 257 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAV 436
KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAV
Sbjct: 72 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAV 131
Query: 437 AFVPISGWHGDNMLEPSTKMPWFKDGRWSVKKAKLTENASLKLSMPSCHLP--APLTSPC 610
AFVPISGWHGDNMLE S+KMPWFK W+V++ + + LP P
Sbjct: 132 AFVPISGWHGDNMLEVSSKMPWFKG--WTVERKEGKVEGKCLIEALDAILPPTRPTDKAL 189
Query: 611 VFPCKTYTK--SVVLVPCPSAELKLVC*NQVPLLSLPRNITTEVKSVEMHH 757
P + K + VP E ++ V + P +TTEVKSVEMHH
Sbjct: 190 RLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFA-PAGLTTEVKSVEMHH 239
Score = 150 bits (363), Expect = 2e-38
Identities = 70/70 (100%), Positives = 70/70 (100%)
Frame = +3
Query: 45 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 224
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 225 AGTGEFEAGI 254
AGTGEFEAGI
Sbjct: 61 AGTGEFEAGI 70
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 192 bits (468), Expect = 3e-51
Identities = 104/171 (60%), Positives = 116/171 (67%), Gaps = 4/171 (2%)
Frame = +2
Query: 257 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAV 436
KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAV
Sbjct: 129 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAV 188
Query: 437 AFVPISGWHGDNMLEPSTKMPWFKDGRWSVKKAKLTENASLKLSMPSCHLP--APLTSPC 610
AFVPISGWHGDNMLE S+KMPWFK W+V++ + + LP P
Sbjct: 189 AFVPISGWHGDNMLEVSSKMPWFKG--WTVERKEGKVEGKCLIEALDAILPPTRPTDKAL 246
Query: 611 VFPCKTYTK--SVVLVPCPSAELKLVC*NQVPLLSLPRNITTEVKSVEMHH 757
P + K + VP E ++ V + P +TTEVKSVEMHH
Sbjct: 247 RLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFA-PAGLTTEVKSVEMHH 296
Score = 175 bits (425), Expect = 5e-46
Identities = 82/82 (100%), Positives = 82/82 (100%)
Frame = +3
Query: 9 AQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITG 188
AQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITG
Sbjct: 46 AQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITG 105
Query: 189 TSQADCAVLIVAAGTGEFEAGI 254
TSQADCAVLIVAAGTGEFEAGI
Sbjct: 106 TSQADCAVLIVAAGTGEFEAGI 127
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 182 bits (443), Expect = 3e-48
Identities = 98/171 (57%), Positives = 112/171 (65%), Gaps = 4/171 (2%)
Frame = +2
Query: 257 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAV 436
KNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKKIGYN A+V
Sbjct: 129 KNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASV 188
Query: 437 AFVPISGWHGDNMLEPSTKMPWFKDGRWSVKKAKLTENASLKLSMPSCHLP--APLTSPC 610
AFVPISGWHGDNMLEPS K PW+K W V++ + + LP P
Sbjct: 189 AFVPISGWHGDNMLEPSPKTPWYKG--WKVERKDGNADGKTLIEALDAILPPSRPTDKAL 246
Query: 611 VFPCKTYTK--SVVLVPCPSAELKLVC*NQVPLLSLPRNITTEVKSVEMHH 757
P + K + VP E ++ + + P +TTEVKSVEMHH
Sbjct: 247 RLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFA-PAALTTEVKSVEMHH 296
Score = 171 bits (416), Expect = 6e-45
Identities = 80/82 (97%), Positives = 81/82 (98%)
Frame = +3
Query: 9 AQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITG 188
AQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITG
Sbjct: 46 AQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITG 105
Query: 189 TSQADCAVLIVAAGTGEFEAGI 254
TSQADCAVLIVAAG GEFEAGI
Sbjct: 106 TSQADCAVLIVAAGIGEFEAGI 127
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 177 bits (431), Expect = 9e-47
Identities = 83/92 (90%), Positives = 87/92 (94%)
Frame = +2
Query: 257 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAV 436
KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAV
Sbjct: 56 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAV 115
Query: 437 AFVPISGWHGDNMLEPSTKMPWFKDGRWSVKK 532
AFVPISGWHGDNMLE S+KMPWFK W+V++
Sbjct: 116 AFVPISGWHGDNMLEVSSKMPWFKG--WTVER 145
Score = 117 bits (281), Expect = 1e-28
Identities = 54/54 (100%), Positives = 54/54 (100%)
Frame = +3
Query: 93 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI 254
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI 54
Score = 66.5 bits (155), Expect = 3e-13
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +1
Query: 505 QGWQVERKEGKADGKCLIEALDAILPPARPTDK 603
+GW VERKEGK +GKCLIEALDAILPP RPTDK
Sbjct: 139 KGWTVERKEGKVEGKCLIEALDAILPPTRPTDK 171
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +3
Query: 132 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 236
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.1
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 117 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 218
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.1
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 117 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 218
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic
acetylcholine Apisa7-2 subunit protein.
Length = 461
Score = 21.4 bits (43), Expect = 9.5
Identities = 8/24 (33%), Positives = 13/24 (54%)
Frame = -3
Query: 493 FG*RLQHVVSVPSRNGHESDSSWV 422
FG L H++ V +N + + WV
Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase
protein.
Length = 580
Score = 21.4 bits (43), Expect = 9.5
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +3
Query: 336 WIPLNHHTVSPDLRKSRRKY 395
W+P+N + S +L +R+Y
Sbjct: 443 WLPVNENYKSLNLAAQKREY 462
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 239,991
Number of Sequences: 438
Number of extensions: 5084
Number of successful extensions: 28
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 23875740
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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