Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20318
(598 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 175 5e-45
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 98 7e-22
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 94 1e-20
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 92 5e-20
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 63 3e-11
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 60 2e-10
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 50 3e-07
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 35 0.010
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.073
SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 29 0.39
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.7
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.6
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.6
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 4.8
SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|... 26 4.8
SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 25 6.3
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 175 bits (425), Expect = 5e-45
Identities = 78/84 (92%), Positives = 80/84 (95%)
Frame = +1
Query: 256 GDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKT 435
GDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+
Sbjct: 55 GDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKS 114
Query: 436 NREKMTQIMFETFNTPAMYVAIQA 507
NREKMTQI+FETFN PA YVAIQA
Sbjct: 115 NREKMTQIIFETFNAPAFYVAIQA 138
Score = 109 bits (261), Expect = 4e-25
Identities = 47/52 (90%), Positives = 51/52 (98%)
Frame = +2
Query: 98 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSY 253
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSY
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSY 53
Score = 62.5 bits (145), Expect = 5e-11
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +3
Query: 510 LSLYASGRTTGIVLDSGDGVSHTVPIYEG 596
LSLYASGRTTGIVLDSGDGV+HTVPIYEG
Sbjct: 140 LSLYASGRTTGIVLDSGDGVTHTVPIYEG 168
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 98.3 bits (234), Expect = 7e-22
Identities = 40/96 (41%), Positives = 67/96 (69%)
Frame = +1
Query: 220 RDGRYGTEGLL*GDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 399
+ G + ++ GDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P
Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102
Query: 400 VLLTEAPLNPKTNREKMTQIMFETFNTPAMYVAIQA 507
+LLTE P+NP NREKM + MFE + +YVAIQA
Sbjct: 103 ILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQA 138
Score = 48.0 bits (109), Expect = 1e-06
Identities = 21/35 (60%), Positives = 26/35 (74%)
Frame = +2
Query: 104 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 208
E A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
Score = 48.0 bits (109), Expect = 1e-06
Identities = 19/28 (67%), Positives = 25/28 (89%)
Frame = +3
Query: 510 LSLYASGRTTGIVLDSGDGVSHTVPIYE 593
LSLYA G ++G+V+DSGDGV+H VP+YE
Sbjct: 140 LSLYAQGLSSGVVVDSGDGVTHIVPVYE 167
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 94.3 bits (224), Expect = 1e-20
Identities = 41/85 (48%), Positives = 54/85 (63%), Gaps = 1/85 (1%)
Frame = +1
Query: 256 GDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPK 432
G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP
Sbjct: 77 GNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPP 136
Query: 433 TNREKMTQIMFETFNTPAMYVAIQA 507
NRE +IMFE+FN +Y+A+QA
Sbjct: 137 ENRENTAEIMFESFNCAGLYIAVQA 161
Score = 41.9 bits (94), Expect = 7e-05
Identities = 20/48 (41%), Positives = 30/48 (62%), Gaps = 2/48 (4%)
Frame = +2
Query: 116 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSY 253
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSY 54
Score = 35.1 bits (77), Expect = 0.008
Identities = 13/20 (65%), Positives = 17/20 (85%)
Frame = +3
Query: 537 TGIVLDSGDGVSHTVPIYEG 596
TG V+DSGDGV+H +P+ EG
Sbjct: 180 TGTVVDSGDGVTHIIPVAEG 199
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 92.3 bits (219), Expect = 5e-20
Identities = 40/84 (47%), Positives = 58/84 (69%), Gaps = 1/84 (1%)
Frame = +1
Query: 256 GDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPK 432
G EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEHP+LLTE PL
Sbjct: 59 GSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANI 118
Query: 433 TNREKMTQIMFETFNTPAMYVAIQ 504
N+EK+ + +ET N PA+ ++Q
Sbjct: 119 RNKEKIAEYFYETLNVPALSFSLQ 142
Score = 59.7 bits (138), Expect = 3e-10
Identities = 25/46 (54%), Positives = 33/46 (71%)
Frame = +2
Query: 116 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSY 253
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMF 57
Score = 51.2 bits (117), Expect = 1e-07
Identities = 21/29 (72%), Positives = 27/29 (93%)
Frame = +3
Query: 510 LSLYASGRTTGIVLDSGDGVSHTVPIYEG 596
L+LYAS RTTGIVL+ GDG++H+VPIY+G
Sbjct: 145 LALYASARTTGIVLECGDGLTHSVPIYDG 173
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 63.3 bits (147), Expect = 3e-11
Identities = 29/73 (39%), Positives = 41/73 (56%)
Frame = +1
Query: 286 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKTNREKMTQIMF 465
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 466 ETFNTPAMYVAIQ 504
E+ PA Y+A Q
Sbjct: 123 ESLRCPATYLAKQ 135
Score = 40.3 bits (90), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +2
Query: 101 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 202
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 60.1 bits (139), Expect = 2e-10
Identities = 30/95 (31%), Positives = 53/95 (55%), Gaps = 1/95 (1%)
Frame = +1
Query: 220 RDGRYGTEGLL*GDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 396
RD + L G++ + G ++ + P E +++NWD ME++ +TF +L + EH
Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116
Query: 397 PVLLTEAPLNPKTNREKMTQIMFETFNTPAMYVAI 501
P+ +TE NP R MT+++FE +N P++ I
Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYNAPSVAYGI 151
Score = 35.9 bits (79), Expect = 0.004
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +2
Query: 116 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 211
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 26.6 bits (56), Expect = 2.7
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +3
Query: 534 TTGIVLDSGDGVSHTVPIYEG 596
++GIVL+ G+ SH +P+ G
Sbjct: 165 SSGIVLNLGNAASHVIPVLNG 185
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 50.0 bits (114), Expect = 3e-07
Identities = 20/66 (30%), Positives = 38/66 (57%)
Frame = +1
Query: 310 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKTNREKMTQIMFETFNTPAM 489
+GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA
Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133
Query: 490 YVAIQA 507
Y+ QA
Sbjct: 134 YLTKQA 139
Score = 36.7 bits (81), Expect = 0.003
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +2
Query: 101 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 202
EE+ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 34.7 bits (76), Expect = 0.010
Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 4/72 (5%)
Frame = +1
Query: 301 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKTNREKMTQIMFE 468
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 469 TFNTPAMYVAIQ 504
P +A +
Sbjct: 172 ECQVPGFTIAYE 183
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.9 bits (69), Expect = 0.073
Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +1
Query: 361 FYNELRVAPEEHPVLLTE--APLNPKTNREKMTQIMFETFNTPAM---YVAIQ 504
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 277
Score = 29.5 bits (63), Expect = 0.39
Identities = 19/66 (28%), Positives = 29/66 (43%)
Frame = -2
Query: 333 IPVSDDSVFDGVFEGQDTSFALCLISS*ESFCPIPTITP*WRGLPTIEGNTARGASSPAK 154
+P + +V +TSFAL S S PT + P +E + +S+PA
Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122
Query: 153 PALHIP 136
P L+ P
Sbjct: 123 PQLNSP 128
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 26.6 bits (56), Expect = 2.7
Identities = 15/45 (33%), Positives = 18/45 (40%)
Frame = -2
Query: 231 PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 97
PT+ P P N S P PL+TTN TS+S
Sbjct: 411 PTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 3.6
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +1
Query: 403 LLTEAPLNPKTNREKMTQIMFETFN 477
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 3.6
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = -2
Query: 237 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 145
P+PT P LPT NT + P+ PAL
Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 25.8 bits (54), Expect = 4.8
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -3
Query: 143 TYRSHCLLPTRQLLRRTS*ISEI 75
TYR H +PT LL T +SEI
Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190
>SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 194
Score = 25.8 bits (54), Expect = 4.8
Identities = 20/46 (43%), Positives = 24/46 (52%), Gaps = 4/46 (8%)
Frame = +2
Query: 470 HST-RPPCTSPS---KPALAVRVRSYHRYRAGLRRRCLPHRAHLRR 595
HST R TSP+ + R RS HR+ G RR P+R HL R
Sbjct: 121 HSTSRSRSTSPANRHRRKEKERTRSNHRH--GSHRRHEPYRTHLSR 164
>SPAC20G8.05c |cdc15||cell division control protein
Cdc15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 927
Score = 25.4 bits (53), Expect = 6.3
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = -3
Query: 251 KSPSVPYRPSRPDGGAFPRSRGTR 180
K+PS PY SRP A S TR
Sbjct: 312 KAPSYPYSSSRPSASASLASSPTR 335
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,637,618
Number of Sequences: 5004
Number of extensions: 56416
Number of successful extensions: 184
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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