BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20292 (700 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 139 3e-34 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 132 6e-32 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 118 1e-27 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 113 2e-26 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 93 3e-20 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 51 1e-07 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 42 6e-05 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 33 0.039 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.85 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 26 4.5 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 6.0 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 7.9 SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 25 7.9 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 139 bits (337), Expect = 3e-34 Identities = 63/86 (73%), Positives = 74/86 (86%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSS 435 NP+NTIFDAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SS Sbjct: 60 NPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISS 119 Query: 436 MVLTKMKETAEAYLGKTVQNAVITVP 513 MVL KM+ETAEAYLG V +AV+TVP Sbjct: 120 MVLMKMRETAEAYLGGKVTDAVVTVP 145 Score = 108 bits (260), Expect = 7e-25 Identities = 51/58 (87%), Positives = 53/58 (91%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGT 683 PAYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ GE NVLIFDLGGGT Sbjct: 145 PAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGT 202 Score = 107 bits (257), Expect = 2e-24 Identities = 49/58 (84%), Positives = 53/58 (91%) Frame = +2 Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 132 bits (318), Expect = 6e-32 Identities = 59/86 (68%), Positives = 72/86 (83%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSS 435 NP+NTIFDAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SS Sbjct: 60 NPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISS 119 Query: 436 MVLTKMKETAEAYLGKTVQNAVITVP 513 MVL KM+E+AEA+LG V +AV+TVP Sbjct: 120 MVLLKMRESAEAFLGGKVTDAVVTVP 145 Score = 107 bits (257), Expect = 2e-24 Identities = 49/58 (84%), Positives = 53/58 (91%) Frame = +2 Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61 Score = 105 bits (252), Expect = 6e-24 Identities = 50/58 (86%), Positives = 52/58 (89%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGT 683 PAYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ E NVLIFDLGGGT Sbjct: 145 PAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGT 202 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 118 bits (283), Expect = 1e-27 Identities = 53/86 (61%), Positives = 66/86 (76%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSS 435 NP NT+FDAKRLIGR++ED Q D+KHWPF+V+ + G P I+V Y GE K F +E+S+ Sbjct: 64 NPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISA 123 Query: 436 MVLTKMKETAEAYLGKTVQNAVITVP 513 MVLTKMKE +EA L K V+ AVITVP Sbjct: 124 MVLTKMKEISEAKLNKRVEKAVITVP 149 Score = 101 bits (241), Expect = 1e-22 Identities = 48/58 (82%), Positives = 51/58 (87%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGT 683 PAYF+DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K +NVLIFDLGGGT Sbjct: 149 PAYFSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGGT 206 Score = 95.5 bits (227), Expect = 7e-21 Identities = 43/58 (74%), Positives = 49/58 (84%) Frame = +2 Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ A P Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 113 bits (272), Expect = 2e-26 Identities = 50/86 (58%), Positives = 68/86 (79%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSS 435 NP NTIFD KRLIGRKF++ T+ D+K +PF +V+D +P ++V G+ K F PEE+S+ Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISA 152 Query: 436 MVLTKMKETAEAYLGKTVQNAVITVP 513 M+L+KMK+TAEAYLGK V +AV+TVP Sbjct: 153 MILSKMKQTAEAYLGKPVTHAVVTVP 178 Score = 100 bits (240), Expect = 2e-22 Identities = 45/58 (77%), Positives = 55/58 (94%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGT 683 PAYFND+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK T E++++++DLGGGT Sbjct: 178 PAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT-EKHIVVYDLGGGT 234 Score = 95.9 bits (228), Expect = 5e-21 Identities = 42/57 (73%), Positives = 49/57 (85%) Frame = +2 Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ P Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNP 94 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 93.1 bits (221), Expect = 3e-20 Identities = 44/58 (75%), Positives = 50/58 (86%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGT 683 PAYFNDSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK + V +FDLGGGT Sbjct: 191 PAYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK--NDAIVAVFDLGGGT 246 Score = 82.2 bits (194), Expect = 6e-17 Identities = 38/86 (44%), Positives = 54/86 (62%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSS 435 NP NT F KRLIGR+F++ VQ D+K P+++V + KT+ P ++ Sbjct: 109 NPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGG 165 Query: 436 MVLTKMKETAEAYLGKTVQNAVITVP 513 +L+KM+ETA YLGK V+NAV+TVP Sbjct: 166 FILSKMRETASTYLGKDVKNAVVTVP 191 Score = 69.3 bits (162), Expect = 5e-13 Identities = 33/66 (50%), Positives = 43/66 (65%), Gaps = 1/66 (1%) Frame = +2 Query: 80 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAR 256 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 257 TPTTQY 274 P + Sbjct: 109 NPENTF 114 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 51.2 bits (117), Expect = 1e-07 Identities = 27/59 (45%), Positives = 36/59 (61%), Gaps = 4/59 (6%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE----RNVLIFDLG 674 PA+F D QR+A +A I+GLN LR++N+ AAA+ YG+ K E R V I D G Sbjct: 149 PAWFTDIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFG 207 Score = 49.2 bits (112), Expect = 6e-07 Identities = 20/59 (33%), Positives = 38/59 (64%) Frame = +2 Query: 77 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61 Score = 41.1 bits (92), Expect = 1e-04 Identities = 26/87 (29%), Positives = 43/87 (49%), Gaps = 1/87 (1%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQ-ADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVS 432 N NT+ KRL GR ++D ++ + ++ G KV Y E+ F ++ Sbjct: 63 NFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLI 122 Query: 433 SMVLTKMKETAEAYLGKTVQNAVITVP 513 + TK+K AEA L +V + VI++P Sbjct: 123 AAYFTKIKAIAEAELIGSVSDVVISIP 149 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 42.3 bits (95), Expect = 6e-05 Identities = 20/64 (31%), Positives = 36/64 (56%) Frame = +3 Query: 495 CSYHGPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLG 674 C P YF D+QR+A + A +GL VL++I++P A +A ++ ++ V++ + G Sbjct: 166 CVMSVPVYFTDAQRKALESAANEAGLPVLQLIHDPAAVILALMYSEEVLIDKTVVVANFG 225 Query: 675 GGTS 686 S Sbjct: 226 ATRS 229 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/55 (30%), Positives = 31/55 (56%) Frame = +2 Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAR 256 +GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ R Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVR 81 Score = 31.5 bits (68), Expect = 0.12 Identities = 22/92 (23%), Positives = 45/92 (48%), Gaps = 6/92 (6%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGED------KTFF 417 N +N++ + + L+G+ ++ T+ H+ V+ G+ K+ + ++ K Sbjct: 82 NADNSVTNFRDLLGKSHDELTLHHC--HYSANPVNVEGQIGFKITVQEDEESDPKEKILT 139 Query: 418 PEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 513 E S L ++ E+AE +LG V V++VP Sbjct: 140 AHEASVRHLRRLTESAEDFLGTKVNGCVMSVP 171 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 33.1 bits (72), Expect = 0.039 Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 1/67 (1%) Frame = +2 Query: 92 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTPTT 268 + ID GT ++ + + G +EI+ R S VAF ER+ G A N R P Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83 Query: 269 QYSMPNV 289 +S+ NV Sbjct: 84 -HSIRNV 89 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.85 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +3 Query: 84 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 179 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 26.2 bits (55), Expect = 4.5 Identities = 28/126 (22%), Positives = 52/126 (41%) Frame = -3 Query: 680 TAAEVKDKYISFSSTLFVKTVSNRSSSGFIDDSENVQA*DGTCIFCGLSLRVIEVRGTVI 501 +++ + + S SS L T S S+S I S + F S + I Sbjct: 565 SSSSIPSTFSSVSSILSSSTSSPSSTSLSISSSST------SSTFSSASTSSPSSISSSI 618 Query: 500 TAFCTVLPR*ASAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSAC 321 ++ T+L + S + +S+ + S +LSS + + + SL+T S+ Sbjct: 619 SSSSTILSSPTPSTSSLMISSSSIISGSSSILSSSIST---IPISSSLSTYSSSVIPSSS 675 Query: 320 TVASSN 303 T+ SS+ Sbjct: 676 TLVSSS 681 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.8 bits (54), Expect = 6.0 Identities = 16/53 (30%), Positives = 26/53 (49%) Frame = -3 Query: 170 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 12 G W SP S + H ++++ +F +RVL C+ L K +K+A L Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/34 (35%), Positives = 17/34 (50%) Frame = +2 Query: 200 AFTDTERLIGDAAKNQVARTPTTQYSMPNVSSDV 301 AF D + G AK A P+T+ + N+S V Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188 >SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 25.4 bits (53), Expect = 7.9 Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 1/51 (1%) Frame = -1 Query: 262 WGSRHLVLGGISDETLGVCERNIRRSGPVALVVGD-DLHLPVLEDTNARVR 113 WGS +G + TLG+ + + + L+VGD LHL V ++ +A +R Sbjct: 418 WGSIGWSVGAMFGATLGIKDSDAPHRRSI-LIVGDGSLHLTV-QEISATIR 466 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,101,320 Number of Sequences: 5004 Number of extensions: 68762 Number of successful extensions: 253 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 228 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 250 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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