BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20288
(635 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |S... 44 2e-05
SPBC887.09c |||leucine-rich repeat protein Sog2 |Schizosaccharom... 42 6e-05
SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 31 0.14
SPBC115.02c |||AFG1 family mitochondrial ATPase|Schizosaccharomy... 30 0.24
SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomy... 28 1.3
SPAC926.06c |||leucine-rich repeat protein, unknown|Schizosaccha... 28 1.3
SPAC1071.11 |||NADH-dependent flavin oxidoreductase |Schizosacch... 27 2.3
SPBC15D4.15 |pho2||4-nitrophenylphosphatase |Schizosaccharomyces... 27 2.3
SPBC3B9.14c |mrpl3||mitochondrial ribosomal protein subunit L3|S... 27 3.0
SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizo... 26 4.0
SPCC1442.04c |||conserved fungal protein|Schizosaccharomyces pom... 25 9.1
SPAC23D3.01 |||PWWP domain protein|Schizosaccharomyces pombe|chr... 25 9.1
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 25 9.1
>SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 690
Score = 44.0 bits (99), Expect = 2e-05
Identities = 22/39 (56%), Positives = 25/39 (64%)
Frame = +3
Query: 513 LPPEIGNLKNLQILSMRENDLIEVPRELGQLARLRELHL 629
LPPEIG LKNL IL N + +P ELG L LRE+ L
Sbjct: 196 LPPEIGKLKNLVILDASGNSIKTIPPELGLLTELREVLL 234
Score = 33.5 bits (73), Expect = 0.026
Identities = 15/41 (36%), Positives = 27/41 (65%)
Frame = +3
Query: 513 LPPEIGNLKNLQILSMRENDLIEVPRELGQLARLRELHLQG 635
+PPE+G L L+ + + +N + +P ELG L +L+ L ++G
Sbjct: 219 IPPELGLLTELREVLLFDNMISVIPAELGTLFQLKILGIEG 259
>SPBC887.09c |||leucine-rich repeat protein Sog2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 886
Score = 42.3 bits (95), Expect = 6e-05
Identities = 26/70 (37%), Positives = 37/70 (52%)
Frame = +1
Query: 304 PVSLSSLPKLRILNVSLNKLYNLPRGFGSFPVLEILDLTYNNLNEKVLPGNFFIMDSLRA 483
P SL L L IL++S NK+ LP FG+ L++L ++ N L E LP M +L
Sbjct: 91 PESLCRLESLEILDISRNKIKQLPESFGALMNLKVLSISKNRLFE--LPTYIAHMPNLEI 148
Query: 484 LYLGDNDFDF 513
L + +N F
Sbjct: 149 LKIENNHIVF 158
Score = 34.3 bits (75), Expect = 0.015
Identities = 20/58 (34%), Positives = 30/58 (51%)
Frame = +1
Query: 328 KLRILNVSLNKLYNLPRGFGSFPVLEILDLTYNNLNEKVLPGNFFIMDSLRALYLGDN 501
+LR LN+ N L P LEILD++ N + K LP +F + +L+ L + N
Sbjct: 76 RLRYLNIRSNVLREFPESLCRLESLEILDISRNKI--KQLPESFGALMNLKVLSISKN 131
>SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1692
Score = 31.1 bits (67), Expect = 0.14
Identities = 18/44 (40%), Positives = 25/44 (56%), Gaps = 2/44 (4%)
Frame = +1
Query: 304 PVSLSSLPK--LRILNVSLNKLYNLPRGFGSFPVLEILDLTYNN 429
P+ S L + L+ LN++ NKL+ LP L LDL+YNN
Sbjct: 493 PLIFSGLSRNSLKELNIANNKLFFLPHSTRYLVNLTYLDLSYNN 536
Score = 30.3 bits (65), Expect = 0.24
Identities = 17/39 (43%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +1
Query: 313 LSSLPKLRILNVSLNKLYN-LPRGFGSFPVLEILDLTYN 426
+ SL KL+ L + N L N LP+ G LE +DL+YN
Sbjct: 567 IGSLVKLKHLYLQFNDLSNRLPQEIGLLKNLETIDLSYN 605
Score = 25.4 bits (53), Expect = 6.9
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +3
Query: 513 LPPEIGNLKNLQILSMRENDLIEVPRELGQLARLRELHL 629
LP EIG LKNL+ + + N + + L + +L +++
Sbjct: 587 LPQEIGLLKNLETIDLSYNAITNI-ASLSECPKLNSINV 624
>SPBC115.02c |||AFG1 family mitochondrial ATPase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 454
Score = 30.3 bits (65), Expect = 0.24
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = -3
Query: 261 VAQLASAAGTTEILLWLRXRRVMFSRENKPGISSRLDIPLSTKSI 127
+ Q + A IL W+ + MFSR+ P ++S L +P K I
Sbjct: 77 ITQDSMPAEKGSILSWISPLKKMFSRKKSPTLTSSLPVPGMPKGI 121
>SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 385
Score = 27.9 bits (59), Expect = 1.3
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = -3
Query: 180 NKPGISSRLDIPLSTKSISGLFISLASSITFLAFDILPVF 61
NKP D+ S +G+F SL T+ A D LP +
Sbjct: 201 NKPAYFGSKDLKQSAPLFTGIFSSLDDIPTYSASDYLPAY 240
>SPAC926.06c |||leucine-rich repeat protein,
unknown|Schizosaccharomyces pombe|chr 1|||Manual
Length = 621
Score = 27.9 bits (59), Expect = 1.3
Identities = 13/33 (39%), Positives = 22/33 (66%)
Frame = +3
Query: 531 NLKNLQILSMRENDLIEVPRELGQLARLRELHL 629
+L++L L + N+L E+P LG+L +L L+L
Sbjct: 354 SLQSLVSLDLSGNELTEIPYALGELPQLCSLNL 386
>SPAC1071.11 |||NADH-dependent flavin oxidoreductase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 244
Score = 27.1 bits (57), Expect = 2.3
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -2
Query: 484 KHGDCP**RSSQVILFHSNYCMLDPIS 404
+HG P SS ++ ++ NYC P+S
Sbjct: 210 EHGSSPLESSSGLVYYNRNYCSTSPLS 236
>SPBC15D4.15 |pho2||4-nitrophenylphosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 298
Score = 27.1 bits (57), Expect = 2.3
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = -1
Query: 407 ISSTGKEPKPLGKLYSLFNETLRILNLGSDDK 312
I STG++PK LGK Y E + I N+ D K
Sbjct: 208 IFSTGRQPKILGKPYDEMMEAI-IANVNFDRK 238
>SPBC3B9.14c |mrpl3||mitochondrial ribosomal protein subunit
L3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 326
Score = 26.6 bits (56), Expect = 3.0
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +1
Query: 349 SLNKLYNLPRGFGSFPVLEILDLTYNNLNEKVLPG 453
S KL+++ + FG F VLE + Y L E+ + G
Sbjct: 81 SNEKLWSIGKVFGDFYVLEYIKCKYPRLPEEAVHG 115
>SPCC1739.11c |cdc11||SIN component scaffold protein
Cdc11|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1045
Score = 26.2 bits (55), Expect = 4.0
Identities = 12/33 (36%), Positives = 22/33 (66%)
Frame = +2
Query: 146 GISSLEEIPGLFSLENITRLXLSHNKISVVPAA 244
G + +EEI + SL+N+ L L +NK++ + A+
Sbjct: 743 GNNEIEEIEEISSLQNLMVLQLDNNKLTNLKAS 775
>SPCC1442.04c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 409
Score = 25.0 bits (52), Expect = 9.1
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = +3
Query: 564 ENDLIEVPRELGQLA 608
ENDL EVP EL +LA
Sbjct: 145 ENDLDEVPTELNELA 159
>SPAC23D3.01 |||PWWP domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 407
Score = 25.0 bits (52), Expect = 9.1
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = -1
Query: 431 KLLYVRSNISSTGKEPKPLGKLYSLFNETLRILNLGSDDKLTGSS 297
++L + N S+ E KLYSLF +++ ++ D+K SS
Sbjct: 318 QILEMIENFSAINSELLESTKLYSLFTLLVKLSDIPLDEKYDFSS 362
>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1284
Score = 25.0 bits (52), Expect = 9.1
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 4/57 (7%)
Frame = -1
Query: 488 YKARRLSIIKKFP-GNTFS---FKLLYVRSNISSTGKEPKPLGKLYSLFNETLRILN 330
YK +R SI++KFP N S LL + + T K+ + L ++ N +L +L+
Sbjct: 178 YKEKRPSIVEKFPLHNLLSRWIHSLLIKSISYAQTEKQEAKVTPLLAIINMSLVLLS 234
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,518,577
Number of Sequences: 5004
Number of extensions: 49265
Number of successful extensions: 148
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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