BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20287 (693 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 315 3e-88 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 311 4e-87 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 196 1e-52 EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 168 5e-44 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003 AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2 DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 2.8 AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.8 D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.6 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 22 4.8 AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cycl... 22 6.4 AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 8.4 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 315 bits (773), Expect = 3e-88 Identities = 150/154 (97%), Positives = 151/154 (98%) Frame = +1 Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 232 DKLKAERDVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 411 DKLKAER+ GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120 Query: 412 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVTK 513 GEFEAGISKNGQTREHALLAFTLGVKQLIVGV K Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154 Score = 113 bits (271), Expect = 2e-27 Identities = 51/55 (92%), Positives = 52/55 (94%) Frame = +3 Query: 507 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPK 671 NKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE+ K Sbjct: 153 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSK 207 Score = 28.7 bits (61), Expect = 0.055 Identities = 9/10 (90%), Positives = 10/10 (100%) Frame = +2 Query: 662 STKMPWFKGW 691 S+KMPWFKGW Sbjct: 205 SSKMPWFKGW 214 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 311 bits (764), Expect = 4e-87 Identities = 148/154 (96%), Positives = 150/154 (97%) Frame = +1 Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 232 DKLKAERDVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 411 DKLKAER+ GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120 Query: 412 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVTK 513 GEFEAGISKNGQTREHALLAFTLGVKQLIVGV K Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154 Score = 107 bits (256), Expect = 1e-25 Identities = 48/55 (87%), Positives = 50/55 (90%) Frame = +3 Query: 507 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPK 671 NKMD T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWHGDNMLE PK Sbjct: 153 NKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPK 207 Score = 24.2 bits (50), Expect = 1.2 Identities = 7/10 (70%), Positives = 8/10 (80%) Frame = +2 Query: 662 STKMPWFKGW 691 S K PW+KGW Sbjct: 205 SPKTPWYKGW 214 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 196 bits (479), Expect = 1e-52 Identities = 94/97 (96%), Positives = 95/97 (97%) Frame = +1 Query: 223 WVLDKLKAERDVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 402 WVLDKLKAER+ GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60 Query: 403 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVTK 513 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGV K Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 97 Score = 113 bits (271), Expect = 2e-27 Identities = 51/55 (92%), Positives = 52/55 (94%) Frame = +3 Query: 507 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPK 671 NKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE+ K Sbjct: 96 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSK 150 Score = 28.7 bits (61), Expect = 0.055 Identities = 9/10 (90%), Positives = 10/10 (100%) Frame = +2 Query: 662 STKMPWFKGW 691 S+KMPWFKGW Sbjct: 148 SSKMPWFKGW 157 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 168 bits (408), Expect = 5e-44 Identities = 80/81 (98%), Positives = 80/81 (98%) Frame = +1 Query: 271 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 450 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60 Query: 451 REHALLAFTLGVKQLIVGVTK 513 REHALLAFTLGVKQLIVGV K Sbjct: 61 REHALLAFTLGVKQLIVGVNK 81 Score = 113 bits (271), Expect = 2e-27 Identities = 51/55 (92%), Positives = 52/55 (94%) Frame = +3 Query: 507 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPK 671 NKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE+ K Sbjct: 80 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSK 134 Score = 28.7 bits (61), Expect = 0.055 Identities = 9/10 (90%), Positives = 10/10 (100%) Frame = +2 Query: 662 STKMPWFKGW 691 S+KMPWFKGW Sbjct: 132 SSKMPWFKGW 141 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 33.1 bits (72), Expect = 0.003 Identities = 16/35 (45%), Positives = 18/35 (51%) Frame = +1 Query: 310 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 414 VT +D PGH FI G D VL+VAA G Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229 Score = 25.4 bits (53), Expect = 0.52 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +1 Query: 64 KTHINIVVIGHVDSGKST 117 K H + ++GHVD GK+T Sbjct: 143 KRHPIVTIMGHVDHGKTT 160 >AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methyltransferase protein. Length = 683 Score = 24.2 bits (50), Expect = 1.2 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +1 Query: 73 INIVVIGHVDSGKST 117 INI IGHV GKST Sbjct: 43 INIGTIGHVAHGKST 57 >DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monooxygenase protein. Length = 517 Score = 23.0 bits (47), Expect = 2.8 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = -2 Query: 65 FSLPIFG*SRITNCV*Y 15 FSLPIFG I +C+ Y Sbjct: 57 FSLPIFGTRWIFSCIGY 73 >AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 23.0 bits (47), Expect = 2.8 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +1 Query: 295 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 396 T KYY D P + FIKN+ ++ +D LI Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327 >D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 22.6 bits (46), Expect = 3.6 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +1 Query: 295 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 396 T KYY D P + FIKN+ ++ +D L+ Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 22.2 bits (45), Expect = 4.8 Identities = 9/29 (31%), Positives = 14/29 (48%) Frame = -2 Query: 686 P*TKAFWLKLQHVVSVPSRNGHESDSSWV 600 P F L L H++ V +N + + WV Sbjct: 30 PLAVVFGLSLHHIIDVDEKNQILTTNCWV 58 >AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cyclase beta-3 protein. Length = 832 Score = 21.8 bits (44), Expect = 6.4 Identities = 6/14 (42%), Positives = 11/14 (78%) Frame = +2 Query: 629 FWMARRQHVGASTK 670 +W+ +R+H +STK Sbjct: 593 YWLEKREHRSSSTK 606 >AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein. Length = 580 Score = 21.4 bits (43), Expect = 8.4 Identities = 7/20 (35%), Positives = 13/20 (65%) Frame = +1 Query: 514 WIPLNHHTVSPDLRKSRRKY 573 W+P+N + S +L +R+Y Sbjct: 443 WLPVNENYKSLNLAAQKREY 462 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 200,037 Number of Sequences: 438 Number of extensions: 4102 Number of successful extensions: 29 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 29 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 21195810 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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