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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20283
         (801 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g39170.1 68417.m05547 SEC14 cytosolic factor, putative / phos...    33   0.29 
At3g60860.1 68416.m06808 guanine nucleotide exchange family prot...    31   0.89 
At2g21520.1 68415.m02561 SEC14 cytosolic factor, putative / phos...    31   1.2  
At5g02370.1 68418.m00160 kinesin motor protein-related kinesin, ...    30   1.6  
At2g21540.1 68415.m02563 SEC14 cytosolic factor, putative / phos...    29   3.6  
At1g01960.1 68414.m00114 guanine nucleotide exchange family prot...    29   4.7  
At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative / phos...    28   6.3  

>At4g39170.1 68417.m05547 SEC14 cytosolic factor, putative /
           phosphoglyceride transfer protein, putative similar to
           phosphatidylinositol transfer-like protein IV
           (GI:14486707) [Lotus japonicus] and
           phosphatidylinositol-phosphatidylcholine transfer
           protein SEC14, Yarrowia lipolytica, PIR2:S43745;contains
           Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
           CRAL/TRIO, N-terminus
          Length = 614

 Score = 32.7 bits (71), Expect = 0.29
 Identities = 17/45 (37%), Positives = 24/45 (53%)
 Frame = +2

Query: 548 IMDVKDISLKFITKFNVSVAKKLSKYQEDAMPIRLKQIHIVNAPP 682
           I+DV+ + LK  TK    +  +L K   D  P  L Q+ I+NA P
Sbjct: 227 ILDVQGVGLKNFTKSARELITRLQKIDGDNYPETLHQMFIINAGP 271


>At3g60860.1 68416.m06808 guanine nucleotide exchange family protein
            similar to guanine nucleotide exchange factor, Homo
            sapiens, GI:5456754; contains Pfam profile PF01369: Sec7
            domain
          Length = 1793

 Score = 31.1 bits (67), Expect = 0.89
 Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 2/35 (5%)
 Frame = +3

Query: 339  RDPSSPPMKKALQILNLAHYEIPGRRFVW--IWQL 437
            R PS+P +    +I+ +AHY +   R VW  IWQ+
Sbjct: 1102 RSPSNPRVFSLTKIVEIAHYNMNRIRLVWSSIWQV 1136


>At2g21520.1 68415.m02561 SEC14 cytosolic factor, putative /
           phosphoglyceride transfer protein, putative contains
           Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
           CRAL/TRIO, N-terminus; similar to phosphatidylinositol
           transfer-like protein IV (GI:14486707) [Lotus japonicus]
          Length = 631

 Score = 30.7 bits (66), Expect = 1.2
 Identities = 16/45 (35%), Positives = 23/45 (51%)
 Frame = +2

Query: 548 IMDVKDISLKFITKFNVSVAKKLSKYQEDAMPIRLKQIHIVNAPP 682
           I+DV+ + LK   K    +  +L K   D  P  L Q+ I+NA P
Sbjct: 233 ILDVQGVGLKNFNKSARDLITRLQKIDGDNYPETLHQMFIINAGP 277


>At5g02370.1 68418.m00160 kinesin motor protein-related kinesin,
           Xenopus laevis, EMBL:XLA249840
          Length = 628

 Score = 30.3 bits (65), Expect = 1.6
 Identities = 14/47 (29%), Positives = 23/47 (48%)
 Frame = +1

Query: 607 KETIEISGGRNADTSEADSHCERPSVCRQIVRVVEAFMKQEITNMIH 747
           +  + + GG   DTSE   + + P  CR     ++AF  +E  N+ H
Sbjct: 43  RSCVSVIGGDGGDTSEVAVYLKDPDSCRNESYQLDAFYGREDDNVKH 89


>At2g21540.1 68415.m02563 SEC14 cytosolic factor, putative /
           phosphoglyceride transfer protein, putative similar to
           phosphatidylinositol transfer-like protein IV
           (GI:14486707) [Lotus japonicus];  contains Pfam PF00650
           : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
           N-terminus
          Length = 548

 Score = 29.1 bits (62), Expect = 3.6
 Identities = 13/43 (30%), Positives = 24/43 (55%)
 Frame = +2

Query: 548 IMDVKDISLKFITKFNVSVAKKLSKYQEDAMPIRLKQIHIVNA 676
           I+DV+ + LK  +K    + +++ K   D  P  L ++ I+NA
Sbjct: 214 ILDVQGVGLKSFSKAARDLLQRIQKIDSDNYPETLNRMFIINA 256


>At1g01960.1 68414.m00114 guanine nucleotide exchange family protein
            similar to guanine nucleotide exchange factor [Homo
            sapiens] GI:5456754; contains Pfam profile PF01369: Sec7
            domain
          Length = 1750

 Score = 28.7 bits (61), Expect = 4.7
 Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
 Frame = +3

Query: 339  RDPSSPPMKKALQILNLAHYEIPGRRFVW--IWQL 437
            R PS P +    +I+ +AHY +   R VW  IW +
Sbjct: 1093 RSPSDPRVFSLTKIVEIAHYNMNRIRLVWSSIWHV 1127


>At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative /
           phosphoglyceride transfer protein, putative similar to
           SP:P24859 from [Kluyveromyces lactissimilar to
           phosphatidylinositol transfer-like protein IV
           (GI:14486707) [Lotus japonicus]; contains Pfam PF00650 :
           CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
           N-terminus
          Length = 608

 Score = 28.3 bits (60), Expect = 6.3
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = +2

Query: 548 IMDVKDISLKFITKFNVSVAKKLSKYQEDAMPIRLKQIHIVNA 676
           I+DV+ + LK  TK    +  +L K   D  P  L ++ I+NA
Sbjct: 223 ILDVQGLGLKNFTKTARDLIIQLQKIDSDNYPETLHRMFIINA 265


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,393,053
Number of Sequences: 28952
Number of extensions: 326834
Number of successful extensions: 802
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 782
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 802
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1814318400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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