BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20283 (801 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g39170.1 68417.m05547 SEC14 cytosolic factor, putative / phos... 33 0.29 At3g60860.1 68416.m06808 guanine nucleotide exchange family prot... 31 0.89 At2g21520.1 68415.m02561 SEC14 cytosolic factor, putative / phos... 31 1.2 At5g02370.1 68418.m00160 kinesin motor protein-related kinesin, ... 30 1.6 At2g21540.1 68415.m02563 SEC14 cytosolic factor, putative / phos... 29 3.6 At1g01960.1 68414.m00114 guanine nucleotide exchange family prot... 29 4.7 At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative / phos... 28 6.3 >At4g39170.1 68417.m05547 SEC14 cytosolic factor, putative / phosphoglyceride transfer protein, putative similar to phosphatidylinositol transfer-like protein IV (GI:14486707) [Lotus japonicus] and phosphatidylinositol-phosphatidylcholine transfer protein SEC14, Yarrowia lipolytica, PIR2:S43745;contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus Length = 614 Score = 32.7 bits (71), Expect = 0.29 Identities = 17/45 (37%), Positives = 24/45 (53%) Frame = +2 Query: 548 IMDVKDISLKFITKFNVSVAKKLSKYQEDAMPIRLKQIHIVNAPP 682 I+DV+ + LK TK + +L K D P L Q+ I+NA P Sbjct: 227 ILDVQGVGLKNFTKSARELITRLQKIDGDNYPETLHQMFIINAGP 271 >At3g60860.1 68416.m06808 guanine nucleotide exchange family protein similar to guanine nucleotide exchange factor, Homo sapiens, GI:5456754; contains Pfam profile PF01369: Sec7 domain Length = 1793 Score = 31.1 bits (67), Expect = 0.89 Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 2/35 (5%) Frame = +3 Query: 339 RDPSSPPMKKALQILNLAHYEIPGRRFVW--IWQL 437 R PS+P + +I+ +AHY + R VW IWQ+ Sbjct: 1102 RSPSNPRVFSLTKIVEIAHYNMNRIRLVWSSIWQV 1136 >At2g21520.1 68415.m02561 SEC14 cytosolic factor, putative / phosphoglyceride transfer protein, putative contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus; similar to phosphatidylinositol transfer-like protein IV (GI:14486707) [Lotus japonicus] Length = 631 Score = 30.7 bits (66), Expect = 1.2 Identities = 16/45 (35%), Positives = 23/45 (51%) Frame = +2 Query: 548 IMDVKDISLKFITKFNVSVAKKLSKYQEDAMPIRLKQIHIVNAPP 682 I+DV+ + LK K + +L K D P L Q+ I+NA P Sbjct: 233 ILDVQGVGLKNFNKSARDLITRLQKIDGDNYPETLHQMFIINAGP 277 >At5g02370.1 68418.m00160 kinesin motor protein-related kinesin, Xenopus laevis, EMBL:XLA249840 Length = 628 Score = 30.3 bits (65), Expect = 1.6 Identities = 14/47 (29%), Positives = 23/47 (48%) Frame = +1 Query: 607 KETIEISGGRNADTSEADSHCERPSVCRQIVRVVEAFMKQEITNMIH 747 + + + GG DTSE + + P CR ++AF +E N+ H Sbjct: 43 RSCVSVIGGDGGDTSEVAVYLKDPDSCRNESYQLDAFYGREDDNVKH 89 >At2g21540.1 68415.m02563 SEC14 cytosolic factor, putative / phosphoglyceride transfer protein, putative similar to phosphatidylinositol transfer-like protein IV (GI:14486707) [Lotus japonicus]; contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus Length = 548 Score = 29.1 bits (62), Expect = 3.6 Identities = 13/43 (30%), Positives = 24/43 (55%) Frame = +2 Query: 548 IMDVKDISLKFITKFNVSVAKKLSKYQEDAMPIRLKQIHIVNA 676 I+DV+ + LK +K + +++ K D P L ++ I+NA Sbjct: 214 ILDVQGVGLKSFSKAARDLLQRIQKIDSDNYPETLNRMFIINA 256 >At1g01960.1 68414.m00114 guanine nucleotide exchange family protein similar to guanine nucleotide exchange factor [Homo sapiens] GI:5456754; contains Pfam profile PF01369: Sec7 domain Length = 1750 Score = 28.7 bits (61), Expect = 4.7 Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 2/35 (5%) Frame = +3 Query: 339 RDPSSPPMKKALQILNLAHYEIPGRRFVW--IWQL 437 R PS P + +I+ +AHY + R VW IW + Sbjct: 1093 RSPSDPRVFSLTKIVEIAHYNMNRIRLVWSSIWHV 1127 >At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative / phosphoglyceride transfer protein, putative similar to SP:P24859 from [Kluyveromyces lactissimilar to phosphatidylinositol transfer-like protein IV (GI:14486707) [Lotus japonicus]; contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus Length = 608 Score = 28.3 bits (60), Expect = 6.3 Identities = 15/43 (34%), Positives = 23/43 (53%) Frame = +2 Query: 548 IMDVKDISLKFITKFNVSVAKKLSKYQEDAMPIRLKQIHIVNA 676 I+DV+ + LK TK + +L K D P L ++ I+NA Sbjct: 223 ILDVQGLGLKNFTKTARDLIIQLQKIDSDNYPETLHRMFIINA 265 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,393,053 Number of Sequences: 28952 Number of extensions: 326834 Number of successful extensions: 802 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 782 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 802 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1814318400 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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