Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20274
(784 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g43920.1 68416.m04701 ribonuclease III family protein similar... 32 0.49
At2g30780.1 68415.m03753 pentatricopeptide (PPR) repeat-containi... 31 0.65
At5g65910.1 68418.m08296 BSD domain-containing protein contains ... 31 0.86
At5g60800.1 68418.m07628 heavy-metal-associated domain-containin... 31 0.86
At3g61390.2 68416.m06872 U-box domain-containing protein several... 31 0.86
At4g02710.1 68417.m00366 kinase interacting family protein simil... 30 1.5
At5g29624.1 68418.m03640 DC1 domain-containing protein contains ... 29 2.6
At1g51070.1 68414.m05741 basic helix-loop-helix (bHLH) family pr... 29 2.6
At1g33060.2 68414.m04076 no apical meristem (NAM) family protein... 29 2.6
At1g33060.1 68414.m04075 no apical meristem (NAM) family protein... 29 2.6
At1g27850.1 68414.m03413 expressed protein similar to En/Spm-lik... 29 2.6
At5g54050.1 68418.m06722 DC1 domain-containing protein 29 3.5
At1g12380.1 68414.m01431 expressed protein 29 3.5
At2g30410.1 68415.m03704 tubulin folding cofactor A (KIESEL) ide... 29 4.6
At5g35604.1 68418.m04242 hypothetical protein 28 6.1
At5g01220.1 68418.m00027 UDP-sulfoquinovose:DAG sulfoquinovosylt... 28 8.0
At3g49690.1 68416.m05433 myb family transcription factor contain... 28 8.0
At2g40435.1 68415.m04988 expressed protein 28 8.0
At1g06230.2 68414.m00659 DNA-binding bromodomain-containing prot... 28 8.0
At1g06230.1 68414.m00658 DNA-binding bromodomain-containing prot... 28 8.0
>At3g43920.1 68416.m04701 ribonuclease III family protein similar to
RNA helicase/RNAseIII CAF protein [Arabidopsis thaliana]
GI:6102610; contains Pfam profiles PF02170: PAZ domain,
PF00636: RNase3 domain
Length = 1531
Score = 31.9 bits (69), Expect = 0.49
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +1
Query: 451 SRKCQTVLENGTLVPSKLTTPGQLATFP 534
SRK Q + NG P + T PGQ + FP
Sbjct: 1022 SRKLQGYIRNGAFEPRRWTAPGQFSLFP 1049
>At2g30780.1 68415.m03753 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 452
Score = 31.5 bits (68), Expect = 0.65
Identities = 17/46 (36%), Positives = 26/46 (56%)
Frame = +3
Query: 357 TQNPEHHEDAEKIVSSVKNDINTAEIALRQGFQEVSDGIGKWYART 494
T P HH D + VS +K+++ T + ++ FQ V D G+W RT
Sbjct: 20 TSLPAHHTDLVQRVSILKDELLTIGNS-KEKFQNVLDQKGQWLFRT 64
>At5g65910.1 68418.m08296 BSD domain-containing protein contains
Pfam profile PF03909: BSD domain
Length = 432
Score = 31.1 bits (67), Expect = 0.86
Identities = 13/52 (25%), Positives = 23/52 (44%)
Frame = +3
Query: 318 LRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAEIALRQGFQEVSDGI 473
L P + Q P+H E+ E +++ +KND R G ++S +
Sbjct: 64 LSQPSSSPDLQERNQTPDHPEEDEDLIAGIKNDFAEIGGRFRTGISKLSGNL 115
>At5g60800.1 68418.m07628 heavy-metal-associated domain-containing
protein similar to farnesylated protein ATFP3
[GI:4097547]; contains Pfam profile PF00403:
Heavy-metal-associated domain
Length = 283
Score = 31.1 bits (67), Expect = 0.86
Identities = 19/58 (32%), Positives = 32/58 (55%)
Frame = +3
Query: 249 VESDLENVEVPDENEEIKRPLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDIN 422
V L+ V++ ++ EE + VDL +P P + +E E +N ++ ED +K K D N
Sbjct: 67 VTGALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKN-KNDEDKKKSEEKKKPDNN 123
>At3g61390.2 68416.m06872 U-box domain-containing protein several
hypothetical proteins - Arabidopsis thaliana
Length = 435
Score = 31.1 bits (67), Expect = 0.86
Identities = 18/73 (24%), Positives = 32/73 (43%), Gaps = 1/73 (1%)
Frame = +3
Query: 276 VPDENEEIKRPLVDLRN-PGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAEIALRQGF 452
+ +E EE+K L ++ G + +E T N H E + + + +DI GF
Sbjct: 319 IREEQEELKIKLREVSKLKGKREEEEASTSN--HREPPQYFICPITHDIMEDPHVAADGF 376
Query: 453 QEVSDGIGKWYAR 491
+ I +W+ R
Sbjct: 377 TYEGEAISRWFER 389
>At4g02710.1 68417.m00366 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 1111
Score = 30.3 bits (65), Expect = 1.5
Identities = 24/88 (27%), Positives = 38/88 (43%), Gaps = 1/88 (1%)
Frame = +2
Query: 494 RAN*RLQASLQHFQENFGAQIQKLNE-TLHFIKPADTIAAPSVEETQNKASFETIESGLK 670
R+N L + L E G Q QKL E +K + A + + + +F+T L+
Sbjct: 447 RSNQNLHSELDSLLEKLGNQSQKLTEKQTELVKLWSCVQAEHLHFQEAETAFQT----LQ 502
Query: 671 SLETNFNSGLNQLSEGIQIVATFKADGE 754
L + LN L+ +Q V+ D E
Sbjct: 503 QLHSQSQEELNNLAVELQTVSQIMKDME 530
>At5g29624.1 68418.m03640 DC1 domain-containing protein contains
Pfam PF03107: DC1 domain
Length = 488
Score = 29.5 bits (63), Expect = 2.6
Identities = 16/33 (48%), Positives = 18/33 (54%)
Frame = +3
Query: 258 DLENVEVPDENEEIKRPLVDLRNPGPPQHQEHE 356
DLE +VPDE EEIK P + G H HE
Sbjct: 261 DLE--DVPDEEEEIKDPFKVINENGDIVHISHE 291
>At1g51070.1 68414.m05741 basic helix-loop-helix (bHLH) family
protein similar to bHLH transcription factor GI:3757520
from [Arabidopsis thaliana]
Length = 226
Score = 29.5 bits (63), Expect = 2.6
Identities = 23/96 (23%), Positives = 48/96 (50%)
Frame = +1
Query: 70 PDKVPEAEDKPLNVVDNLSSEQELIDQANTIKDIDNSLRANKKEVIDIPVKVIVEEIKPS 249
P + P+ DK + D + + D+A +KD+++SL+ KE+ D K + + K
Sbjct: 95 PGRTPKT-DKVAIINDAIRMVNQARDEAQKLKDLNSSLQEKIKELKD--EKNELRDEKQK 151
Query: 250 LKVI*KTLKCRMKMRKSRGL*SI*EIPGPRSIKSTK 357
LKV + + ++K K++ +P P+++ +
Sbjct: 152 LKVEKERIDQQLKAIKTQPQPQPCFLPNPQTLSQAQ 187
>At1g33060.2 68414.m04076 no apical meristem (NAM) family protein
similar to NAC1 GB:AAF68626 GI:7716952 from (Medicago
truncatula)
Length = 652
Score = 29.5 bits (63), Expect = 2.6
Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -3
Query: 569 HSTFVSERRNF-LGNVASWPGVVNLLGTSVPF 477
H+ ++R++F L N+ WPG LL T PF
Sbjct: 344 HNESSNDRKDFVLPNMMHWPGNTRLLSTEYPF 375
>At1g33060.1 68414.m04075 no apical meristem (NAM) family protein
similar to NAC1 GB:AAF68626 GI:7716952 from (Medicago
truncatula)
Length = 648
Score = 29.5 bits (63), Expect = 2.6
Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -3
Query: 569 HSTFVSERRNF-LGNVASWPGVVNLLGTSVPF 477
H+ ++R++F L N+ WPG LL T PF
Sbjct: 344 HNESSNDRKDFVLPNMMHWPGNTRLLSTEYPF 375
>At1g27850.1 68414.m03413 expressed protein similar to En/Spm-like
transposon protein GB:AAB95292 GI:2088658 from
[Arabidopsis thaliana]
Length = 1148
Score = 29.5 bits (63), Expect = 2.6
Identities = 16/50 (32%), Positives = 30/50 (60%), Gaps = 1/50 (2%)
Frame = +3
Query: 246 VVESDLENVEVPDENEE-IKRPLVDLRNPGPPQHQEHETQNPEHHEDAEK 392
V +S++EN+ PD++EE I +V+L N +H+ HE+ + D ++
Sbjct: 448 VFQSEVENLAYPDKHEESIAFGMVNLSNES-SRHESHESSFSDQLGDMDQ 496
>At5g54050.1 68418.m06722 DC1 domain-containing protein
Length = 580
Score = 29.1 bits (62), Expect = 3.5
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = +3
Query: 261 LENVEVPDENEEIKRPLVDLRNPGPPQHQEHE 356
LE +VP+E EEIK P + G H HE
Sbjct: 272 LELEDVPEEEEEIKDPFKVINEKGDIVHFSHE 303
>At1g12380.1 68414.m01431 expressed protein
Length = 793
Score = 29.1 bits (62), Expect = 3.5
Identities = 17/71 (23%), Positives = 36/71 (50%)
Frame = +3
Query: 306 PLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAEIALRQGFQEVSDGIGKWY 485
PL +R+ + +PE +D +K+++ + + + A IAL + + ++G+ Y
Sbjct: 609 PLYLIRDSSGKYLPPFKCLSPEQEKDVDKLITRLVSR-DEAHIALMELMKWRTEGLDPMY 667
Query: 486 ARTEQINDSRP 518
AR Q+ + P
Sbjct: 668 ARAVQMKERDP 678
>At2g30410.1 68415.m03704 tubulin folding cofactor A (KIESEL)
identical to cDNA tubulin folding cofactor A,
GI:20514256, SP|O04350 Tubulin-specific chaperone A
(Tubulin-folding cofactor A) (CFA) (TCP1-chaperonin
cofactor A homolog) {Arabidopsis thaliana}
Length = 113
Score = 28.7 bits (61), Expect = 4.6
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = +3
Query: 276 VPDENEEIKRPLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAE 431
+PD ++ ++ L DL++ + E + PE EDA+K V+ V+ T +
Sbjct: 62 IPDCHKRLESALADLKSTLAELEETDEKEGPE-IEDAKKTVADVEKQFPTED 112
>At5g35604.1 68418.m04242 hypothetical protein
Length = 298
Score = 28.3 bits (60), Expect = 6.1
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +2
Query: 44 CSPHAQPPSRTRSPRPKI 97
CSP PPSRT P P +
Sbjct: 92 CSPRLSPPSRTMGPPPPV 109
>At5g01220.1 68418.m00027 UDP-sulfoquinovose:DAG
sulfoquinovosyltransferase / sulfolipid synthase (SQD2)
identical to GI:20302857
Length = 510
Score = 27.9 bits (59), Expect = 8.0
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +1
Query: 43 VLAARAASIPDKVPEAEDKPLNVVDNLSSEQELIDQANTI 162
V+AARA IPD +PE ++ + N ++ + + T+
Sbjct: 403 VVAARAGGIPDIIPEDQEGKTGFLFNPGDVEDCVTKLRTL 442
>At3g49690.1 68416.m05433 myb family transcription factor contains
PFAM profile: myb DNA binding domain PF00249
Length = 310
Score = 27.9 bits (59), Expect = 8.0
Identities = 19/83 (22%), Positives = 38/83 (45%)
Frame = +3
Query: 285 ENEEIKRPLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAEIALRQGFQEVS 464
+ E++K L+ +N + QE E NP HH+D+ +++ + ++ +++ L +
Sbjct: 185 DQEDVKPVLI--KNMVKIEDQELEKTNPHHHQDS---MTNAFDHLSFSQLLLDPNHNHLG 239
Query: 465 DGIGKWYARTEQINDSRPACNIS 533
G G N + P N S
Sbjct: 240 SGEGFSMNSILSANTNSPLLNTS 262
>At2g40435.1 68415.m04988 expressed protein
Length = 158
Score = 27.9 bits (59), Expect = 8.0
Identities = 19/66 (28%), Positives = 27/66 (40%)
Frame = +2
Query: 554 IQKLNETLHFIKPADTIAAPSVEETQNKASFETIESGLKSLETNFNSGLNQLSEGIQIVA 733
IQKL + + T S E T K T+E+ K N SG NQ + ++
Sbjct: 40 IQKLKQKVERFNQDPTAEQSSSEPTDPKTPMVTVETLDKGFMINVFSGKNQPGMLVSVLE 99
Query: 734 TFKADG 751
F+ G
Sbjct: 100 AFEDIG 105
>At1g06230.2 68414.m00659 DNA-binding bromodomain-containing protein
contains bromodomain, INTERPRO:IPR001487
Length = 766
Score = 27.9 bits (59), Expect = 8.0
Identities = 14/66 (21%), Positives = 32/66 (48%)
Frame = +3
Query: 237 NQTVVESDLENVEVPDENEEIKRPLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKND 416
++ + E+ EN+ D ++ I +PLV+ + PQ + + E+ + ++D
Sbjct: 93 DKNLTEAPSENLPGDDSDKVIDKPLVEAFSQAQPQ-DDASLAAMDKSEEVPSQIPKAQDD 151
Query: 417 INTAEI 434
+NT +
Sbjct: 152 VNTVVV 157
>At1g06230.1 68414.m00658 DNA-binding bromodomain-containing protein
contains bromodomain, INTERPRO:IPR001487
Length = 766
Score = 27.9 bits (59), Expect = 8.0
Identities = 14/66 (21%), Positives = 32/66 (48%)
Frame = +3
Query: 237 NQTVVESDLENVEVPDENEEIKRPLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKND 416
++ + E+ EN+ D ++ I +PLV+ + PQ + + E+ + ++D
Sbjct: 93 DKNLTEAPSENLPGDDSDKVIDKPLVEAFSQAQPQ-DDASLAAMDKSEEVPSQIPKAQDD 151
Query: 417 INTAEI 434
+NT +
Sbjct: 152 VNTVVV 157
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,039,302
Number of Sequences: 28952
Number of extensions: 295337
Number of successful extensions: 1230
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1229
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1755792000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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