BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20259 (633 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 132 3e-32 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 132 4e-32 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 114 1e-26 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 98 1e-21 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 95 7e-21 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 53 3e-08 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 46 5e-06 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 31 0.10 SPAC1556.07 |pmm1||phosphomannomutase Pmm1|Schizosaccharomyces p... 30 0.24 SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces po... 27 1.7 SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 26 3.9 SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ... 26 3.9 SPBC16C6.05 |||translation initiation factor |Schizosaccharomyce... 25 6.9 SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1... 25 6.9 SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch... 25 9.1 SPBC18E5.01 ||SPBC29A3.19|cycloisomerase 2 family|Schizosaccharo... 25 9.1 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 132 bits (320), Expect = 3e-32 Identities = 66/85 (77%), Positives = 68/85 (80%) Frame = +3 Query: 255 LLLDVTPLSLGIETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQVFEGERAMTK 434 LLLDV PLS+GIETAGGVMT LIKRN YSDNQPGVLIQVFEGERA TK Sbjct: 390 LLLDVAPLSMGIETAGGVMTPLIKRNTTIPTKKSEIFSTYSDNQPGVLIQVFEGERARTK 449 Query: 435 DNNLLGKFELTGIPPAPRGVPQIEV 509 D NLLGKFEL+GIPPAPRGVPQIEV Sbjct: 450 DCNLLGKFELSGIPPAPRGVPQIEV 474 Score = 109 bits (262), Expect = 3e-25 Identities = 53/85 (62%), Positives = 63/85 (74%) Frame = +1 Query: 10 FRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEXX 189 FR TMEPVE+ LRD+K+DKA +++IVLVGGSTRIP+VQKL+ DFFNGKE KSINPDE Sbjct: 308 FRKTMEPVERVLRDSKVDKASVNEIVLVGGSTRIPRVQKLVSDFFNGKEPCKSINPDEAV 367 Query: 190 XXXXXXXXXILHGDKSEEVQDLCCL 264 +L GD SE+ QDL L Sbjct: 368 AYGAAVQAAVLTGDTSEKTQDLLLL 392 Score = 70.5 bits (165), Expect = 2e-13 Identities = 33/41 (80%), Positives = 36/41 (87%) Frame = +2 Query: 509 TFDIFANGILNVSAIEKSTNKENKITITNDKGRLSKEEIER 631 TFD+ ANGILNVSA+EK T K KITITNDKGRLSKEEI+R Sbjct: 475 TFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEIDR 515 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 132 bits (319), Expect = 4e-32 Identities = 66/85 (77%), Positives = 68/85 (80%) Frame = +3 Query: 255 LLLDVTPLSLGIETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQVFEGERAMTK 434 LLLDV PLSLGIETAGGVMT LIKRN Y+DNQPGVLIQVFEGERA TK Sbjct: 390 LLLDVAPLSLGIETAGGVMTPLIKRNTTIPTKKSEVFSTYADNQPGVLIQVFEGERARTK 449 Query: 435 DNNLLGKFELTGIPPAPRGVPQIEV 509 D NLLGKFEL+GIPPAPRGVPQIEV Sbjct: 450 DCNLLGKFELSGIPPAPRGVPQIEV 474 Score = 109 bits (262), Expect = 3e-25 Identities = 53/85 (62%), Positives = 64/85 (75%) Frame = +1 Query: 10 FRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEXX 189 FR+TMEPVEK LRD+K+DK+ +++IVLVGGSTRIP++QKL+ DFFNGKE KSINPDE Sbjct: 308 FRNTMEPVEKVLRDSKIDKSSVNEIVLVGGSTRIPRIQKLVSDFFNGKEPCKSINPDEAV 367 Query: 190 XXXXXXXXXILHGDKSEEVQDLCCL 264 IL GD SE+ QDL L Sbjct: 368 AYGAAVQAAILVGDTSEKTQDLLLL 392 Score = 70.5 bits (165), Expect = 2e-13 Identities = 33/41 (80%), Positives = 36/41 (87%) Frame = +2 Query: 509 TFDIFANGILNVSAIEKSTNKENKITITNDKGRLSKEEIER 631 TFD+ ANGILNVSA+EK T K KITITNDKGRLSKEEI+R Sbjct: 475 TFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEIDR 515 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 114 bits (274), Expect = 1e-26 Identities = 58/90 (64%), Positives = 63/90 (70%) Frame = +3 Query: 240 GGAGSLLLDVTPLSLGIETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQVFEGE 419 G +LLDV PL+LGIET GGVMT LI RN DNQ VLIQV+EGE Sbjct: 414 GSDNIVLLDVIPLTLGIETTGGVMTKLIGRNTPIPTRKSQIFSTAVDNQNTVLIQVYEGE 473 Query: 420 RAMTKDNNLLGKFELTGIPPAPRGVPQIEV 509 R +TKDNNLLGKF+L GIPPAPRGVPQIEV Sbjct: 474 RTLTKDNNLLGKFDLRGIPPAPRGVPQIEV 503 Score = 81.4 bits (192), Expect = 1e-16 Identities = 38/78 (48%), Positives = 55/78 (70%) Frame = +1 Query: 10 FRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEXX 189 F+ T++PVE+ L+D+ + K++I DIVLVGGSTRIPKVQ+LL+ FF GK+ +K INPDE Sbjct: 340 FKKTLKPVEQVLKDSNLKKSEIDDIVLVGGSTRIPKVQELLESFF-GKKASKGINPDEAV 398 Query: 190 XXXXXXXXXILHGDKSEE 243 +L G++ + Sbjct: 399 AYGAAVQAGVLSGEEGSD 416 Score = 53.2 bits (122), Expect = 3e-08 Identities = 25/42 (59%), Positives = 33/42 (78%), Gaps = 1/42 (2%) Frame = +2 Query: 509 TFDIFANGILNVSAIEKS-TNKENKITITNDKGRLSKEEIER 631 TF++ ANG+L VSA++KS K K+ I NDKGRLS+E+IER Sbjct: 504 TFEVDANGVLTVSAVDKSGKGKPEKLVIKNDKGRLSEEDIER 545 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 97.9 bits (233), Expect = 1e-21 Identities = 48/85 (56%), Positives = 57/85 (67%) Frame = +3 Query: 255 LLLDVTPLSLGIETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQVFEGERAMTK 434 +LLDVTPLSLGIET GGV T LI RN +D Q V I+VF+GER + + Sbjct: 433 VLLDVTPLSLGIETLGGVFTRLINRNTTIPTRKSQVFSTAADGQTAVEIRVFQGERELVR 492 Query: 435 DNNLLGKFELTGIPPAPRGVPQIEV 509 DN L+G F+LTGI PAP+G PQIEV Sbjct: 493 DNKLIGNFQLTGIAPAPKGQPQIEV 517 Score = 57.2 bits (132), Expect = 2e-09 Identities = 25/72 (34%), Positives = 46/72 (63%) Frame = +1 Query: 13 RSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEXXX 192 R T++P +++L+DA + ++I++++LVGG TR+P+V + ++ F +E KS+NPDE Sbjct: 357 RRTIDPCKRALKDANLQTSEINEVILVGGMTRMPRVVETVKSIFK-REPAKSVNPDEAVA 415 Query: 193 XXXXXXXXILHG 228 +L G Sbjct: 416 IGAAIQGGVLSG 427 Score = 43.2 bits (97), Expect = 3e-05 Identities = 20/40 (50%), Positives = 30/40 (75%) Frame = +2 Query: 509 TFDIFANGILNVSAIEKSTNKENKITITNDKGRLSKEEIE 628 +FD+ A+GI+NVSA +K+TNK++ IT+ G L+ EIE Sbjct: 518 SFDVDADGIINVSARDKATNKDSSITVAGSSG-LTDSEIE 556 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 95.1 bits (226), Expect = 7e-21 Identities = 46/86 (53%), Positives = 62/86 (72%), Gaps = 1/86 (1%) Frame = +1 Query: 10 FRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEXX 189 F++T++PV K L+D+K+ KA +HDIVLVGGSTRIPKVQ+L+ DFF+G+ LNKSINPDE Sbjct: 312 FKATIDPVAKVLKDSKVPKADVHDIVLVGGSTRIPKVQRLVSDFFDGRALNKSINPDEAV 371 Query: 190 XXXXXXXXXIL-HGDKSEEVQDLCCL 264 +L + S++ QDL L Sbjct: 372 AYGAAVQAAVLTNKADSDKTQDLLLL 397 Score = 72.1 bits (169), Expect = 6e-14 Identities = 36/84 (42%), Positives = 46/84 (54%) Frame = +3 Query: 255 LLLDVTPLSLGIETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQVFEGERAMTK 434 LLLDV PLSLG+ G V + RN +DNQ V V++GER Sbjct: 395 LLLDVVPLSLGVAMEGNVFGVVCPRNTPIPTIKKRTFTTVADNQTTVTFPVYQGERVNCA 454 Query: 435 DNNLLGKFELTGIPPAPRGVPQIE 506 +N LG+F+LTGIPP PRG ++E Sbjct: 455 ENEPLGEFQLTGIPPMPRGQAELE 478 Score = 44.0 bits (99), Expect = 2e-05 Identities = 20/40 (50%), Positives = 28/40 (70%) Frame = +2 Query: 509 TFDIFANGILNVSAIEKSTNKENKITITNDKGRLSKEEIE 628 TF++ ANGIL V+A+EK+T + I ITN G LS +I+ Sbjct: 480 TFELDANGILKVTAVEKTTGRSAHIEITNSVGHLSSTKIQ 519 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 53.2 bits (122), Expect = 3e-08 Identities = 20/56 (35%), Positives = 40/56 (71%) Frame = +1 Query: 16 STMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDE 183 + +EP+ K+L+ A + ++I+ I+L GG++RIP +Q L D+ + +++K++N DE Sbjct: 346 AAVEPINKALKKANLTFSEINSIILFGGASRIPFIQSTLADYVSSDKISKNVNADE 401 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 46.0 bits (104), Expect = 5e-06 Identities = 21/52 (40%), Positives = 37/52 (71%) Frame = +1 Query: 28 PVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDE 183 P+EK+L A + K ++ I +VGG TR+P V++++ ++F GK L+ ++N DE Sbjct: 322 PIEKALELAGIKKEDLYSIEMVGGCTRVPIVKEVIANYF-GKGLSFTLNQDE 372 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 31.5 bits (68), Expect = 0.10 Identities = 12/46 (26%), Positives = 27/46 (58%) Frame = +1 Query: 31 VEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKS 168 V +++ A M+ I +++L GG++ PK+ L++ F + + +S Sbjct: 341 VTEAVEKANMEPFDISEVILAGGASNTPKLTSLMESIFPEQTIIRS 386 >SPAC1556.07 |pmm1||phosphomannomutase Pmm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 257 Score = 30.3 bits (65), Expect = 0.24 Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 1/35 (2%) Frame = +2 Query: 506 GTFDIFANGILNVSAIEKSTNKENKITITN-DKGR 607 GTF F NG++N+S + ++ N E + DKGR Sbjct: 127 GTFIEFRNGMINISPVGRNANTEERNEFERFDKGR 161 >SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 27.5 bits (58), Expect = 1.7 Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 1/36 (2%) Frame = +1 Query: 529 RYPQRFRYREVHQQGEQDHHYQRQRSS-LQGRDRAY 633 R P R +YRE H + + H R QGR +Y Sbjct: 320 RDPDRTKYREYHSERRKQHRTDRYSDDYYQGRSYSY 355 >SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 932 Score = 26.2 bits (55), Expect = 3.9 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = -3 Query: 79 RGFEPYPSWHHGETSPLAP 23 + +P P+ HG+TSPL+P Sbjct: 420 KAIKPMPTSIHGQTSPLSP 438 >SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 2|||Manual Length = 827 Score = 26.2 bits (55), Expect = 3.9 Identities = 13/28 (46%), Positives = 14/28 (50%) Frame = +1 Query: 331 TLPSPLNRLRHSPPTLITNPEYSSKYLR 414 T P RLRH+P I E KYLR Sbjct: 43 TYGQPFKRLRHAPEKYIEFLELRLKYLR 70 >SPBC16C6.05 |||translation initiation factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 190 Score = 25.4 bits (53), Expect = 6.9 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = +2 Query: 536 LNVSAIEKSTNKENKITITNDKGRLSKEEIER 631 LNVS + S +E +T ++ R+ +EE +R Sbjct: 63 LNVSGTKDSNAEEQPAKLTKEEKRVEREEAKR 94 >SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1023 Score = 25.4 bits (53), Expect = 6.9 Identities = 23/88 (26%), Positives = 38/88 (43%), Gaps = 1/88 (1%) Frame = +1 Query: 217 ILHGDKSEEVQDLCCLM*HPF-PSVLRLLEVS*PHSSSVTLPSPLNRLRHSPPTLITNPE 393 IL D ++ V+ + +M H + S + L E ++ PSP+ L +++ Sbjct: 754 ILVPDPAKRVR-MSAIMNHAYLRSHVSLFETYNDDEATSLRPSPIRLLSQKSLASVSSVS 812 Query: 394 YSSKYLRVSVL*PKITTCSVNSS*PGSH 477 S Y L P+IT S+N P H Sbjct: 813 SISTYSSSIELTPQITKTSLNREKPALH 840 >SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 509 Score = 25.0 bits (52), Expect = 9.1 Identities = 11/32 (34%), Positives = 22/32 (68%) Frame = +2 Query: 527 NGILNVSAIEKSTNKENKITITNDKGRLSKEE 622 +GIL+ + IEK +K K+ + N+ G++S ++ Sbjct: 154 DGILSFTVIEKVDDKNLKVRV-NNNGKISSKK 184 >SPBC18E5.01 ||SPBC29A3.19|cycloisomerase 2 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 342 Score = 25.0 bits (52), Expect = 9.1 Identities = 12/41 (29%), Positives = 18/41 (43%) Frame = -2 Query: 476 WDPGQLEFTEQVVIFGHSTLTLKYLDEYSGLVIRVGGECLS 354 WD G+ ++ I GHS +L L+ S L +S Sbjct: 61 WDNGKFSLVDRFTIDGHSPTSLCVLENGSVLTANYNSASIS 101 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,667,881 Number of Sequences: 5004 Number of extensions: 54853 Number of successful extensions: 240 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 212 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 237 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 281707720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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