BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20256 (795 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC336.10c |tif512||translation initiation factor|Schizosacchar... 109 4e-25 SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizos... 109 4e-25 SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 4.1 SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|ch... 27 4.1 SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 5.4 SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizo... 26 5.4 SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac... 26 7.1 SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 26 7.1 SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosa... 25 9.4 >SPBC336.10c |tif512||translation initiation factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 109 bits (262), Expect = 4e-25 Identities = 48/65 (73%), Positives = 56/65 (86%) Frame = +2 Query: 59 MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHL 238 M + E FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+MSTSKTGKHGHAKVH+ Sbjct: 1 MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHI 60 Query: 239 VGIDI 253 V +DI Sbjct: 61 VALDI 65 Score = 107 bits (258), Expect = 1e-24 Identities = 45/87 (51%), Positives = 67/87 (77%) Frame = +1 Query: 256 NGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQL 435 NG+KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM +G ++D+++P+G+LG ++ Sbjct: 67 NGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGELGNEI 125 Query: 436 RTDFDSGKELLCTVLKSCGEECVIASK 516 F+ GK+L+ TV+ + GEE +A + Sbjct: 126 EEGFEEGKDLIITVVSAMGEEIALACR 152 >SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 109 bits (262), Expect = 4e-25 Identities = 48/65 (73%), Positives = 56/65 (86%) Frame = +2 Query: 59 MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHL 238 M + E FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+MSTSKTGKHGHAKVH+ Sbjct: 1 MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHI 60 Query: 239 VGIDI 253 V +DI Sbjct: 61 VALDI 65 Score = 108 bits (260), Expect = 8e-25 Identities = 46/92 (50%), Positives = 68/92 (73%) Frame = +1 Query: 256 NGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQL 435 NG+KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM +G ++D+++P+G+LG ++ Sbjct: 67 NGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGELGNEI 125 Query: 436 RTDFDSGKELLCTVLKSCGEECVIASKQTQLS 531 FD G++L+ TV+ + GEE +A + S Sbjct: 126 EEGFDEGRDLIITVVSAMGEETALACRDAPSS 157 >SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1729 Score = 26.6 bits (56), Expect = 4.1 Identities = 9/23 (39%), Positives = 15/23 (65%) Frame = +1 Query: 295 NMDVPHVKREDYQLTDISDDGYL 363 NM +P+++ E Q+ D + D YL Sbjct: 968 NMSIPYIRGEYVQMIDANQDNYL 990 >SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 204 Score = 26.6 bits (56), Expect = 4.1 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +1 Query: 151 FRYAEGSSMQDC*NVHIQNRKARPR*SSLGWD 246 + + E +Q C N+ +N +A R SS GWD Sbjct: 41 YHHLEKGLLQQCFNLVKKNMEALYRQSSFGWD 72 >SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 26.2 bits (55), Expect = 5.4 Identities = 12/39 (30%), Positives = 17/39 (43%) Frame = +3 Query: 9 AVGHISFLTVVKFKTQQWVTSKTHTSRPETPGPQPPSPC 125 AVG + L + W+ K +T+ ET PQ C Sbjct: 154 AVGFVDALFCTRIPASLWIKYKEYTTSAETTVPQECGLC 192 >SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1513 Score = 26.2 bits (55), Expect = 5.4 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = +1 Query: 649 NNTEVVLGDRGAPPLADVTRNTSP 720 N+ + L DR PP+ +R TSP Sbjct: 1059 NSASLSLNDRPGPPMEPASRETSP 1082 >SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 340 Score = 25.8 bits (54), Expect = 7.1 Identities = 15/30 (50%), Positives = 19/30 (63%) Frame = +1 Query: 472 TVLKSCGEECVIASKQTQLSTNKPTQHL*G 561 TV K+ EECV+AS +T L T PT + G Sbjct: 306 TVAKT--EECVLASIETFLDTGIPTNPVNG 333 >SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||Manual Length = 3971 Score = 25.8 bits (54), Expect = 7.1 Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 2/40 (5%) Frame = -2 Query: 506 MTHSSPQDFSTVHNNSLPLSKS--VRNCVPRSPSGILRSS 393 + S+P STV N+S P++ S + +P + S +L SS Sbjct: 1254 LNSSTPITSSTVVNSSTPITSSTALNTSIPITSSSVLNSS 1293 Score = 25.4 bits (53), Expect = 9.4 Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 2/48 (4%) Frame = -2 Query: 506 MTHSSPQDFSTVHNNSLPLSKS--VRNCVPRSPSGILRSSRRSPLSAI 369 + S+P STV N+S P++ S + P + S +L SS S+I Sbjct: 3138 LNSSTPITSSTVVNSSTPITSSTTLNTSTPITSSSVLNSSTAITSSSI 3185 >SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosaccharomyces pombe|chr 1|||Manual Length = 875 Score = 25.4 bits (53), Expect = 9.4 Identities = 10/17 (58%), Positives = 14/17 (82%) Frame = +1 Query: 313 VKREDYQLTDISDDGYL 363 +KREDY+LT ++ GYL Sbjct: 91 IKREDYELTLCANCGYL 107 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,227,971 Number of Sequences: 5004 Number of extensions: 67070 Number of successful extensions: 238 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 207 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 236 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 387388442 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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