Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20256
(795 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 104 8e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 101 4e-22
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 101 6e-22
At3g06450.1 68416.m00746 anion exchange family protein similar t... 31 1.2
At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 29 2.7
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 3.6
At5g41460.1 68418.m05035 fringe-related protein strong similarit... 29 4.7
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 4.7
At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 8.2
At2g27680.1 68415.m03354 aldo/keto reductase family protein cont... 28 8.2
At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 8.2
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 104 bits (249), Expect = 8e-23
Identities = 47/86 (54%), Positives = 65/86 (75%), Gaps = 1/86 (1%)
Frame = +1
Query: 262 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLR 438
KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Q++
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIK 127
Query: 439 TDFDSGKELLCTVLKSCGEECVIASK 516
+ FD GK+L+ +V+ + GEE + A K
Sbjct: 128 SGFDDGKDLVVSVMSAMGEEQINALK 153
Score = 101 bits (241), Expect = 7e-22
Identities = 42/72 (58%), Positives = 55/72 (76%)
Frame = +2
Query: 71 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 250
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 251 ISMVKSMKISVP 286
I K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 101 bits (243), Expect = 4e-22
Identities = 46/87 (52%), Positives = 65/87 (74%), Gaps = 1/87 (1%)
Frame = +1
Query: 262 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 438
KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L TQL+
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLK 127
Query: 439 TDFDSGKELLCTVLKSCGEECVIASKQ 519
F+ GK+++ +V+ + GEE + A K+
Sbjct: 128 NGFEEGKDIVVSVMSAMGEEQMCALKE 154
Score = 97.5 bits (232), Expect = 9e-21
Identities = 41/72 (56%), Positives = 54/72 (75%)
Frame = +2
Query: 71 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 250
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 251 ISMVKSMKISVP 286
I K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 101 bits (242), Expect = 6e-22
Identities = 45/87 (51%), Positives = 64/87 (73%), Gaps = 1/87 (1%)
Frame = +1
Query: 262 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 438
KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Q+R
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMR 127
Query: 439 TDFDSGKELLCTVLKSCGEECVIASKQ 519
FD GK+++ +V+ S GEE + A K+
Sbjct: 128 LGFDEGKDIVVSVMSSMGEEQICAVKE 154
Score = 93.1 bits (221), Expect = 2e-19
Identities = 39/72 (54%), Positives = 51/72 (70%)
Frame = +2
Query: 71 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 250
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 251 ISMVKSMKISVP 286
I K ++ VP
Sbjct: 64 IFTAKKLEDIVP 75
>At3g06450.1 68416.m00746 anion exchange family protein similar to
putative Anion exchanger family members: GB:AAD39673,
GB:AAD55295 [Arabidopsis thaliana]
Length = 732
Score = 30.7 bits (66), Expect = 1.2
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -3
Query: 673 LRAPPLYYYSFFKLNLYTILC 611
LR PP Y+Y F L TILC
Sbjct: 328 LRKPPAYHYDLFLLGFLTILC 348
>At1g56345.1 68414.m06477 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 322
Score = 29.5 bits (63), Expect = 2.7
Identities = 20/69 (28%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
Frame = -1
Query: 480 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQVTIV-SHQGQVAIIRDISQLVVFALHVGY 304
+HGA + AA++V L +RD E +V V S + + + D++ ++V G
Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232
Query: 303 VHVVCGGTD 277
+ CGG D
Sbjct: 233 RELSCGGDD 241
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 29.1 bits (62), Expect = 3.6
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = +1
Query: 400 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 501
L IPDGD T L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At5g41460.1 68418.m05035 fringe-related protein strong similarity
to unknown protein (pir||T13026) similarity to predicted
proteins + similar to hypothetical protein GB:AAC23643
[Arabidopsis thaliana] + weak similarity to Fringe
[Schistocerca gregaria](GI:6573138);Fringe encodes an
extracellular protein that regulates Notch signalling.
Length = 524
Score = 28.7 bits (61), Expect = 4.7
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +3
Query: 36 VVKFKTQQWVTSKTHTSRPETPGPQPPSP 122
++ F T Q K++ S P +P P PP P
Sbjct: 80 LILFHTNQTAVIKSYASPPPSPPPPPPPP 108
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 4.7
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -1
Query: 171 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 64
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At3g53330.1 68416.m05884 plastocyanin-like domain-containing
protein similar to mavicyanin SP:P80728 from [Cucurbita
pepo]
Length = 310
Score = 27.9 bits (59), Expect = 8.2
Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%)
Frame = +3
Query: 69 SKTHT-SRPETPGPQPPS 119
SKTH SRP TP P PPS
Sbjct: 127 SKTHERSRPITPSPPPPS 144
>At2g27680.1 68415.m03354 aldo/keto reductase family protein
contains Pfam profile PF00248: oxidoreductase, aldo/keto
reductase family
Length = 384
Score = 27.9 bits (59), Expect = 8.2
Identities = 26/95 (27%), Positives = 44/95 (46%)
Frame = +1
Query: 271 EDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFD 450
++I S MDV + + D ++DGYL + DL+E +G + T T+FD
Sbjct: 147 QNIDISRKRMDVAALDMLQFHWWDYANDGYLDALKHLTDLKE-----EGKIKTVALTNFD 201
Query: 451 SGKELLCTVLKSCGEECVIASKQTQLSTNKPTQHL 555
+ E L +L++ G V Q + +P Q +
Sbjct: 202 T--ERLQKILEN-GIPVVSNQVQHSIVDMRPQQRM 233
>At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein
contains INTERPRO domain, IPR001878: Zn-finger CCHC type
Length = 353
Score = 27.9 bits (59), Expect = 8.2
Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%)
Frame = +3
Query: 72 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 197
KT T P PP PCN PC S + + R CP
Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,058,418
Number of Sequences: 28952
Number of extensions: 371470
Number of successful extensions: 1156
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1054
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1141
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1794809600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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